LOCUS       Exported               10236 bp ds-DNA     circular SYN 03-FEB-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 28, 2022 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4611..4898
                     /organism="Homo sapiens"
                     /chromosome="17"
                     /map="17p13.1"
                     /mol_type="mRNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:9606"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        571..774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        572..770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     LTR             788..968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1015..1140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1633..1866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     primer_bind     1654..1671
                     /label=RRE-F
                     /note="color: black; sequence: CTTGGGAGCAGCAGGAAG; added: 
                     2022-01-14"
     misc_feature    2393..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2564..3774
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3611..3630
                     /label=UBC-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
                     /note="color: black; sequence: TGAAGCTCCGGTTTTGAACT"
     regulatory      3822..3831
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             3828..4544
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3828 .. 3830 / #05fd14
                        2: 3831 .. 3833 / #05fd14 / 1a
                        3: 3834 .. 4544 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3873..3894)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(4134..4153)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     4481..4502
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     4524..4538
                     /label=mRuby-F
                     /note="color: black; sequence: ggtggcatggacgagctg; added: 
                     2022-01-14"
     primer_bind     4537..4554
                     /label=BsrGI-2A-F
                     /note="color: black; sequence: ATtgtacaagggaAGCGGAG; added:
                     2022-01-14"
     CDS             4554..4610
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 4607"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4611..4901
                     /codon_start=1
                     /label=VAMP2(1-96)
                     /note="color: #99ccff"
                     /translation="MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDI
                     MRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMM"
     misc_feature    4838^4839
                     /label=BoNT/B cleavage site
                     /note="color: #ff0000"
     primer_bind     complement(4886..4909)
                     /label=EcoRI-VAMP2-R
                     /note="color: black; sequence: tcgaattcttacatcatcttgagg; 
                     added: 2022-01-14"
     misc_feature    4926..5514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(4979..4999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(5397..5408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 5396"
                     /translation="IEGR"
     primer_bind     complement(5517..5533)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5518..5534)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCGAGGTCGACGGTATC"
     LTR             6039..6219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(6245..6262)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    6251..6475
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6521..6949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(6608..6627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     6818..6839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(6958..6978)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        6963..7292
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      7143..7278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     7205..7224
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     promoter        7340..7387
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7406..7780
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7910..8031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7947..7966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     8001..8020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(8080..8096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(8080..8096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(8093..8115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    8104..8120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8128..8158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8128 .. 8134 / #ffffff / -10
                        2: 8135 .. 8152 / #ffffff
                        3: 8153 .. 8158 / #ffffff / -35"
     protein_bind    8173..8194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(8311..8328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(8482..9070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(8562..8581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(9241..10101)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9241 .. 10032 / #ccffcc
                        2: 10033 .. 10101 / #ccffcc / signal sequence
                     Cleavage site after base 10032"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9604..9614)
                     /label=BsrGI-2A-F
                     /note="color: black; sequence: ATtgtacaagggaAGCGGAG; added:
                     2022-01-14"
     primer_bind     9864..9883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(10102..10206)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
       61 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      121 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      181 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
      781 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      841 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      901 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      961 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     1021 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1081 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1141 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1201 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1261 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1321 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1381 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1441 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1501 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1561 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1621 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1681 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1741 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1801 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1861 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1921 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1981 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2041 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2101 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2161 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2221 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2281 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2341 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2401 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2461 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2521 acagggacag cagagatcca gtttggttaa ttaagggtgc agcggcctcc gcgccgggtt
     2581 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     2641 cgcaggagcg ttcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     2701 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     2761 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     2821 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     2881 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     2941 gatcgctgtg atcgtcactt ggtgagttgc gggctgctgg gctggccggg gctttcgtgg
     3001 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     3061 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcacaaaa tggcggctgt
     3121 tcccgagtct tgaatggaag acgcttgtaa ggcgggctgt gaggtcgttg aaacaaggtg
     3181 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     3241 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     3301 gactggagaa ctcgggtttg tcgtctggtt gcgggggcgg cagttatgcg gtgccgttgg
     3361 gcagtgcacc cgtacctttg ggagcgcgcg cctcgtcgtg tcgtgacgtc acccgttctg
     3421 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     3481 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     3541 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     3601 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     3661 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     3721 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct
     3781 tgggctgcag gtcgactcta gaggatcccc gggtaccggt cgccaccatg gtgagcaagg
     3841 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3901 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3961 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4021 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4081 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4141 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4201 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4261 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4321 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4381 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4441 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4501 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggaagc ggagccacga
     4561 acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct atgtctgcta
     4621 ccgctgccac ggccccccct gctgccccgg ctggggaggg tggtccccct gcaccccctc
     4681 caaacctcac cagtaacagg agactgcagc agacccaggc ccaggtggat gaggtggtgg
     4741 acatcatgag ggtgaacgtg gacaaggtcc tggagcgaga ccagaagctg tcggagctgg
     4801 acgaccgtgc agatgcactc caggcggggg cctcccagtt tgaaacaagc gcagccaagc
     4861 tcaagcgcaa atactggtgg aaaaacctca agatgatgta agaattcgat atcaagctta
     4921 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4981 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5041 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5101 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5161 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5221 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5281 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5341 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5401 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5461 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5521 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5581 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5641 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5701 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5761 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5821 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5881 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5941 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     6001 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     6061 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6121 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6181 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     6241 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6301 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6361 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6421 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6481 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6601 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6961 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7021 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7081 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7141 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7201 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7261 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7321 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7381 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7441 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7501 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7561 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7621 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7681 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7741 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7801 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7861 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8041 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8101 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8161 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8221 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10201 tccgcgcaca tttccccgaa aagtgccacc tgacac
//
