LOCUS       pTPH412                 3821 bp    DNA     circular     27-APR-2022
DEFINITION  [pSC101] psp sd8 TadA8e-15aalinker-gp41-8N.
ACCESSION   urn.local...26-eqxw658
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(1..951)
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     source          1..>3821
     rep_origin      complement(955..1700)
     primer_bind     1591..1614
                     /standard_name="AB0399"
     modified_base   1701..1715
                     /note="USER junction"
     regulatory      1716..1760
                     /note="Geneious type: terminator"
                     /note="rrnB1 transcriptional terminator"
     primer_bind     complement(1743..1782)
                     /standard_name="TW388"
     misc_feature    1761..1767
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     modified_base   1768..1782
                     /note="USER junction"
     source          <1768..>1963
     primer_bind     1768..1786
                     /standard_name="TPH1889"
     misc_feature    1775..1781
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used to
                     exchange f1 origin or selection components"
     regulatory      1782..1949
                     /note="Geneious type: promoter"
                     /standard_name="Phage Shock Promoter"
     misc_feature    1785..1794
                     /note
     misc_feature    1801..1817
                     /note
     misc_feature    1805..1814
                     /note
     primer_bind     1860..1900
                     /note
     misc_feature    1881..1888
                     /note
     primer_bind     complement(1881..1920)
                     /note
     misc_feature    1893..1897
                     /note
     misc_feature    1908
                     /note
     primer_bind     complement(1945..1963)
                     /standard_name="TPH1890"
     modified_base   1949..1963
                     /note="USER junction"
     misc_feature    1950..1957
                     /note="SbfI"
     misc_feature    1955..1963
                     /standard_name="USER Junction"
     primer_bind     1955..1998
                     /standard_name="TPH1620"
     misc_feature    1957..1962
                     /note="BsgI"
     misc_feature    1964..1977
                     /note="Geneious type: RBS"
                     /standard_name="sd8"
     source          <1964..>1977
     source          <1978..>2478
     CDS             1981..2478
                     /standard_name="TadAmax10 (ABE8)"
     source          <1981..>2478
     primer_bind     complement(2461..2510)
                     /standard_name="TPH1932"
     misc_feature    2479..2532
                     /note="Geneious type: motif"
                     /standard_name="Linker"
     misc_feature    2496..2510
                     /standard_name="USER Junction"
     primer_bind     2496..2555
                     /standard_name="TPH1933"
     source          <2533..>2814
     misc_feature    2533..2547
                     /standard_name="scar"
     misc_feature    2548..2814
                     /note="Geneious type: motif"
                     /standard_name="gp41-8-N"
     primer_bind     complement(2801..2833)
                     /standard_name="TPH1866"
     source          <2815..>2816
     source          complement(<2815..>2816)
     source          <2815..>2816
                     /organism="pTSara-C"
                     /mol_type="other DNA"
     modified_base   2817..2833
                     /note="USER junction"
     primer_bind     2817..2837
                     /standard_name="TPH1865"
     misc_feature    2819..2826
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used to
                     exchange antibiotic resistance marker or selection
                     components"
     misc_feature    2834..2840
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     regulatory      complement(2841..2882)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
     primer_bind     complement(2874..2895)
                     /standard_name="TW1128"
     modified_base   2883..2895
     source          <2883..3821
     misc_feature    2883..>3821
                     /standard_name="From pTPH295"
     primer_bind     2883..2907
                     /standard_name="AW035"
     CDS             complement(2896..3711)
                     /standard_name="Kan"
     primer_bind     complement(3022..3041)
                     /standard_name=747
     regulatory      complement(3712..3805)
                     /note="Geneious type: promoter"
                     /note="KanR Promoter"
     primer_bind     complement(3804..3821)
                     /standard_name="TPH1731"
     modified_base   3806..3821
                     /note="USER Junction"
     primer_bind     3806..22
                     /standard_name="TPH1867"
ORIGIN      
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctgatgaaat tcgccacttg
     1801 ttagtgtaat tcgctaactc atcctggcat gttgctgttg attcttcaat cagatcttta
     1861 taaatcaaaa agataaaaaa ttggcacgca aattgtatta acagttcagc aggacaatcc
     1921 tgaacgcaga aatcaagagg acaacattac ctgcaggtgc agtaaaggaa aaaaaaaatg
     1981 tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg
     2041 gcacgggatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc
     2101 ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg
     2161 gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac
     2221 gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc
     2281 gtggtgtttg gcgtgagaaa ctcaaaaaga ggcgccgcag gctccctgat gaacgtgctg
     2341 aactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt
     2401 gccgccctgc tgtgcgattt ctatcggatg cctagacagg tgttcaatgc tcagaagaag
     2461 gcccagagct ccatcaacaa ggaatctggc tctgtatctt ctgaacagtt ggcgcaattc
     2521 cgctctctgg acagccagct gaaccgctgc ctgagcctgg ataccatggt ggtgaccaac
     2581 ggcaaagcga ttgaaattcg cgatgtgaaa gtgggcgatt ggctggaaag cgaatgcggc
     2641 ccggtgcagg tgaccgaagt gctgccgatt attaaacagc cggtgtttga aattgtgctg
     2701 aaaagcggca aaaaaattcg cgtgagcgcg aaccataaat ttccgaccaa agatggcctg
     2761 aaaaccatta acagcggcct gaaagtgggc gattttctgc gcagccgcgc gaaataactt
     2821 aattaacggc actcctcagc aaatataatg accctcttga taacccaaga gggcattttt
     2881 taatgcccat ggcgtttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     2941 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     3001 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     3061 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     3121 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
     3181 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     3241 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     3301 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     3361 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     3421 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     3481 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     3541 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     3601 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     3661 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct
     3721 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     3781 tgcaatgtaa catcagagat tttgaaggcc aaataggccg t
//
