LOCUS       pWS3806_-_CHORDS_spacer 1922 bp ds-DNA     circular     15-SEP-2020
DEFINITION  .
KEYWORDS    "E. coli Marker:AmpR" "Sequence Verified" "Type:678"               
FEATURES             Location/Qualifiers
     misc_feature    12..61
                     /label="Spacer 2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(73..169)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(170..1027)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
     promoter        complement(1028..1158)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(1159..1922)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 cgtctctTGC CCCATAAACG TGCTATTAGT TATTAGGTCC GAAGATTGAA AAAGGTGAGG
       61 GCTAAagaga cggattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      301 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1141 cccgaaaagt gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gt
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