LOCUS       pILGFP9E3               5830 bp    DNA     circular     21-Jan-2020
FEATURES             Location/Qualifiers
     CDS             4402..5205
                     /label=KlURA3
                     /note=Detected CDS 267aa
     rep_origin      1996..2615
                     /label=rep
                     /note=rep_origin
     promoter        4236..4401
                     /label=URA3p
                     /note=promoter
     terminator      1074..1373
                     /label=PGK1 terminator
                     /note=terminator
     CDS             351..1073
                     /label=yEGFP
                     /note=CDS
     CDS             354..1064
                     /label=yEGFP
                     /note=CDS
     CDS             complement(2770..3630)
                     /label=ampicillin resistance protein
                     /note=ampicillin resistance protein
     misc_feature    1994..2088
                     /label=ori
                     /note=/SECDrawAs=Region
     misc_feature    1950..1985
                     /label=Gibson Assembly arm
                     /note=misc_feature
     misc_feature    complement(3859..3881)
                     /label=pGEX_3_primer
                     /note=misc_feature
     misc_feature    complement(4080..4223)
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    4194..4216
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        4209..4225
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(1605..1623)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(1622..1644)
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(3672..3700)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     misc_feature    27..132
                     /label=TetO elements
                     /note=misc_feature
     misc_feature    5399..5417
                     /label=TEF1 promoter start
                     /note=misc_feature
     misc_feature    446..471
                     /label=1B1 primer
                     /note=misc_feature
     promoter        5399..5830
                     /label=TEF1 promoter UAS
                     /note=promoter
     promoter        151..341
                     /label=TEF1 core promoter
                     /note=promoter
     promoter        5399..5830
                     /label=TEF1 promoter UAS
                     /note=promoter
     promoter        151..341
                     /label=TEF1 core promoter
                     /note=promoter
     promoter        5399..5830
                     /label=TEF1 promoter part 1
                     /note=promoter
     promoter        151..341
                     /label=TEF1 core promoter
                     /note=promoter
     misc_feature    27..45
                     /label=TetO
                     /note=misc_feature
     misc_feature    54..72
                     /label=TetO
                     /note=misc_feature
     misc_feature    88..106
                     /label=TetO
                     /note=misc_feature
     misc_feature    114..132
                     /label=TetO
                     /note=misc_feature
BASE COUNT     1664 a      1236 c      1283 g      1647 t 
ORIGIN
        1 CTACCTGAAT CTAAGATTGG GCCCTAACTC TATCATTGAT AGAGTAATTG GTATCCCTAT 
       61 CAGTGATAGA GATAAGCGGG GGCGGTATCC CTATCAGTGA TAGAGAGGGC TTAACTCTAT 
      121 CATTGATAGA GTGCCGGCAA TCCTCGAGCA GATTCTTTTT CTTGAAAATT TTTTTTTTTG 
      181 ATTTTTTTCT CTTTCGATGA CCTCCCATTG ATATTTAAGT TAATAAACGG TCTTCAATTT 
      241 CTCAAGTTTC AGTTTCATTT TTCTTGTTCT ATTACAACTT TTTTTACTTC TTGCTCATTA 
      301 GAAAGAAAGC ATAGCAATCT AATCTAAGTT TTAATTACAA AGGATCCAAA ATGTCTAAAG 
      361 GTGAAGAATT ATTCACTGGT GTTGTCCCAA TTTTGGTTGA ATTAGATGGT GATGTTAATG 
      421 GTCACAAATT TTCTGTCTCC GGTGAAGGTG AAGGTGATGC TACTTACGGT AAATTGACCT 
      481 TAAAATTTAT TTGTACTACT GGTAAATTGC CAGTTCCATG GCCAACCTTA GTCACTACTT 
      541 TCGGTTATGG TGTTCAATGT TTTGCGAGAT ACCCAGATCA TATGAAACAA CATGACTTTT 
      601 TCAAGTCTGC CATGCCAGAA GGTTATGTTC AAGAAAGAAC TATTTTTTTC AAAGATGACG 
      661 GTAACTACAA GACCAGAGCT GAAGTCAAGT TTGAAGGTGA TACCTTAGTT AATAGAATCG 
      721 AATTAAAAGG TATTGATTTT AAAGAAGATG GTAACATTTT AGGTCACAAA TTGGAATACA 
      781 ACTATAACTC TCACAATGTT TACATCATGG CTGACAAACA AAAGAATGGT ATCAAAGTTA 
      841 ACTTCAAAAT TAGACACAAC ATTGAAGATG GTTCTGTTCA ATTAGCTGAC CATTATCAAC 
      901 AAAATACTCC AATTGGTGAT GGTCCAGTCT TGTTACCAGA CAACCATTAC TTATCCACTC 
      961 AATCTGCCTT ATCCAAAGAT CCAAACGAAA AGAGAGACCA CATGGTCTTG TTAGAATTTG 
     1021 TTACTGCTGC TGGTATTACC CATGGTATGG ATGAATTGTA CAAAAGATCT TAAATTGAAT 
     1081 TGAATTGAAA TCGATAGATC AATTTTTTTC TTTTCTCTTT CCCCATCCTT TACGCTAAAA 
     1141 TAATAGTTTA TTTTATTTTT TGAATATTTT TTATTTATAT ACGTATATAT AGACTATTAT 
     1201 TTATCTTTTA ATGATTATTA AGATTTTTAT TAAAAAAAAA TTCGCTCCTC TTTTAATGCC 
     1261 TTTATGCAGT TTTTTTTTCC CATTCGATAT TTCTATGTTC GGGTTCAGCG TATTTTAAGT 
     1321 TTAATAACTC GAAAATTCTG CGTTCGTTAA AGCTTTCGAG AAGGATATTA TTTACTAGTA 
     1381 GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG AAAAGCAGGC 
     1441 TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAACTGTATT ATAAGTAAAT 
     1501 GCATGTATAC TAAACTCACA AATTAGAGCT TCAATTTAAT TATATCAGTT ATTACCCGGG 
     1561 AATCTCGGTC GTAATGATTT AAATGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT 
     1621 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 
     1681 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 
     1741 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 
     1801 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 
     1861 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     1921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     1981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     2041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     2101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     2161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     2221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     2281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     2341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     2401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 
     2461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     2521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     2581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     2641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     2701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 
     2761 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 
     2821 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 
     2881 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 
     2941 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 
     3001 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 
     3061 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 
     3121 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 
     3181 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 
     3241 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 
     3301 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 
     3361 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 
     3421 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 
     3481 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 
     3541 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 
     3601 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 
     3661 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 
     3721 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT 
     3781 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT 
     3841 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT 
     3901 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG 
     3961 TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG 
     4021 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT 
     4081 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 
     4141 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC 
     4201 ACGACGTTGT AAAACGACGG CCAGTGAATT CATTTAAATT TTTTTGATTC GGTAATCTCC 
     4261 GAGCAGAAGG AAGAACGAAG GAAGGAGCAC AGACTTAGAT TGGTATATAT ACGCATATGT 
     4321 GGTGTTGAAG AAACATGAAA TTGCCCAGTA TTCTTAACCC AACTGCACAG AACAAAAACC 
     4381 TGCAGGAAAC GAAGATAAAA CATGTCCACA AAATCATATA CCAGTAGAGC TGAGACTCAT 
     4441 GCAAGTCCGG TTGCATCGAA ACTTTTACGT TTAATGGATG AAAAGAAGAC CAATTTGTGT 
     4501 GCTTCTCTTG ACGTTCGTTC GACTGATGAG CTATTGAAAC TTGTTGAAAC GTTGGGTCCA 
     4561 TACATTTGCC TTTTGAAAAC ACACGTTGAT ATCTTGGATG ATTTCAGTTA TGAGGGTACT 
     4621 GTCGTTCCAT TGAAAGCATT GGCAGAGAAA TACAAGTTCT TGATATTTGA GGACAGAAAA 
     4681 TTCGCCGATA TCGGTAACAC AGTCAAATTA CAATATACAT CGGGCGTTTA CCGTATCGCA 
     4741 GAATGGTCTG ATATCACCAA CGCCCACGGG GTTACTGGTG CTGGTATTGT TGCTGGCTTG 
     4801 AAACAAGGTG CGCAAGAGGT CACCAAAGAA CCAAGGGGAT TATTGATGCT TGCTGAATTG 
     4861 TCTTCCAAGG GTTCTCTAGC ACACGGTGAA TATACTAAGG GTACCGTTGA TATTGCAAAG 
     4921 AGTGATAAAG ATTTCGTTAT TGGGTTCATT GCTCAGAACG ATATGGGAGG AAGAGAAGAA 
     4981 GGGTTTGATT GGCTAATCAT GACCCCAGGT GTAGGTTTAG ACGACAAAGG CGATGCATTG 
     5041 GGTCAGCAGT ACAGAACCGT CGACGAAGTT GTAAGTGGTG GATCAGATAT CATCATTGTT 
     5101 GGCAGAGGAC TTTTCGCCAA GGGTAGAGAT CCTAAGGTTG AAGGTGAAAG ATACAGAAAT 
     5161 GCTGGATGGG AAGCGTACCA AAAGAGAATC AGCGCTCCCC ATTAATTATA CAGGAAACTT 
     5221 AATAGAACAA ATCACATATT TAATCTAATA GCCACCTGCA TTGGCACGGT GCAACACTCA 
     5281 CTTCAACTTC ATCTTACAAA AGATCACGTG ATCTGTTGTA TTGGGATCTC TAGACCTAAT 
     5341 AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG TTGCTCGAGA AAGAGCTCCA 
     5401 GAAAGCGACC ACCCAACTTT GGCTGATAAT AGCGTATAAA CAATGCATAC TTTGTACGTT 
     5461 CAAAATACAA TGCAGTAGAT ATATTTATGC ATATTACATA TAATACATAT CACATAGGAA 
     5521 GCAACAGGCG CGTTGGACTT TTAATTTTCG AGGACCGCGA ATCCTTACAT CACACCCAAT 
     5581 CCCCCACAAG TGATCCCCCA CACACCATAG CTTCAAAATG TTTCTACTCC TTTTTTACTC 
     5641 TTCCAGATTT TCTCGGACTC CGCGCATCGC CGTACCACTT CAAAACACCC AAGCACAGCA 
     5701 TACTAAATTT CCCCTCTTTC TTCCTCTAGG GTGTCGTTAA TTACCCGTAC TAAAGGTTTG 
     5761 GAAAAGAAAA AAGAGACCGC CTCGTTTCTT TTTCTTCGTC GAAAAAGGCA ATAAAAATTT 
     5821 TTATCACGTT  
//