LOCUS       MultiLig5452            5452 bp    DNA     circular     30-Apr-2022
FEATURES             Location/Qualifiers
     CDS             4465..5268
                     /label=KlURA3
                     /note=Detected CDS 267aa
     CDS             708..1436
                     /label=yEGFP
                     /note=Detected CDS 242aa
     rep_origin      2059..2678
                     /label=rep
                     /note=rep_origin
     promoter        4299..4464
                     /label=URA3p
                     /note=promoter
     CDS             717..1427
                     /label=yEGFP
                     /note=CDS
     CDS             complement(2833..3693)
                     /label=ampicillin resistance protein
                     /note=ampicillin resistance protein
     misc_feature    2057..2151
                     /label=ori
                     /note=/SECDrawAs=Region
     misc_feature    2013..2048
                     /label=Gibson Assembly arm
                     /note=misc_feature
     misc_feature    complement(3922..3944)
                     /label=pGEX_3_primer
                     /note=misc_feature
     misc_feature    complement(4143..4286)
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    4257..4279
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        4272..4288
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(1668..1686)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(1685..1707)
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(3735..3763)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     misc_feature    809..834
                     /label=1B1 primer
                     /note=misc_feature
     misc_feature    467..480
                     /label=PZ4
                     /note=misc_feature
     misc_feature    483..496
                     /label=PZ4
                     /note=misc_feature
     misc_feature    499..512
                     /label=PZ4
                     /note=misc_feature
     misc_feature    1443..1643
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(2059..2673)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    2833..3693
                     /label=bla
                     /note=misc_feature
     misc_feature    1..707
                     /label=SkGAL2 3*PZ4
                     /note=misc_feature
BASE COUNT     1560 a      1170 c      1287 g      1435 t 
ORIGIN
        1 TAAACCAATT TTATTTGAAC TTGCCCCGCT AATCGTACAG ACGTACCAGA TCCAGGTCAT 
       61 AACGGTGCCA TTAGAAGGCC GTAGAATAGA CCCACTAAAG CTATCACAGG CAACCTTGCA 
      121 TCCAAGTCTA ATTAAACGGA GCAGAATCTG TTCCAGTAAT GTATAAAGAA TTAACTACCG 
      181 GAAAAGCGTC TTCCGGGGCG GCTCAACCGG ATTACATTCT TTTTTCTTTT TTGCGGCCAG 
      241 CATTCGAGTC ACGTGCTCAA ATACTGAGGA AGGGGTTCTT CCGGTAGGCA CCGGCGCTCA 
      301 CTCTTCCGTG CGGCACACTC CCCGCAGTTC AGGGGCCATG TGCCTGTGAG AGTATTTCCG 
      361 CAGGCCATAG TTCCGGGGTG GACCACTCCG AGCTAATGCT AAAGTAAGCC CTTCCCAACT 
      421 TGAAATAGGA ACAAAAGTCA ATTATGCTCC TCCTGTAAAG CTAAACTAGA GTGAGACGTT 
      481 TTTAGAGTGA GACGTTGTTA GAGTGAGACG TTGTTCAAGG GAGGGATGAG TTGAGGAATA 
      541 AAATGCGGAA AAAGTGTGGG TTAGACAGAC ATATATAAAC TCTAGCCATT CGGCTGAAAT 
      601 ATATCTGAGT TCATTTGTGA TAACTTTTTT GATTACTTGT TCATATATCC ACAAGAACAA 
      661 CGACTATTAA ACAATAGAAA ATAGTATAAT AAAAAAAGTT TTTTACAATG GGATCCTCTA 
      721 AAGGTGAAGA ATTATTCACT GGTGTTGTCC CAATTTTGGT TGAATTAGAT GGTGATGTTA 
      781 ATGGTCACAA ATTTTCTGTC TCCGGTGAAG GTGAAGGTGA TGCTACTTAC GGTAAATTGA 
      841 CCTTAAAATT TATTTGTACT ACTGGTAAAT TGCCAGTTCC ATGGCCAACC TTAGTCACTA 
      901 CTTTCGGTTA TGGTGTTCAA TGTTTTGCGA GATACCCAGA TCATATGAAA CAACATGACT 
      961 TTTTCAAGTC TGCCATGCCA GAAGGTTATG TTCAAGAAAG AACTATTTTT TTCAAAGATG 
     1021 ACGGTAACTA CAAGACCAGA GCTGAAGTCA AGTTTGAAGG TGATACCTTA GTTAATAGAA 
     1081 TCGAATTAAA AGGTATTGAT TTTAAAGAAG ATGGTAACAT TTTAGGTCAC AAATTGGAAT 
     1141 ACAACTATAA CTCTCACAAT GTTTACATCA TGGCTGACAA ACAAAAGAAT GGTATCAAAG 
     1201 TTAACTTCAA AATTAGACAC AACATTGAAG ATGGTTCTGT TCAATTAGCT GACCATTATC 
     1261 AACAAAATAC TCCAATTGGT GATGGTCCAG TCTTGTTACC AGACAACCAT TACTTATCCA 
     1321 CTCAATCTGC CTTATCCAAA GATCCAAACG AAAAGAGAGA CCACATGGTC TTGTTAGAAT 
     1381 TTGTTACTGC TGCTGGTATT ACCCATGGTA TGGATGAATT GTACAAAAGA TCTTAAACTA 
     1441 GTAGGACTAT TTGCAAAGGG AAGGGATGCT AAGGTAGAGG GTGAACGTTA CAGAAAAGCA 
     1501 GGCTGGGAAG CATATTTGAG AAGATGCGGC CAGCAAAACT AAAAAACTGT ATTATAAGTA 
     1561 AATGCATGTA TACTAAACTC ACAAATTAGA GCTTCAATTT AATTATATCA GTTATTACCC 
     1621 GGGAATCTCG GTCGTAATGA TTTAAATGCA TGCAAGCTTG GCGTAATCAT GGTCATAGCT 
     1681 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 
     1741 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 
     1801 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 
     1861 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 
     1921 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     1981 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     2041 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     2101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     2161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     2221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     2281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     2341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     2401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     2461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 
     2521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     2581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     2641 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     2701 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     2761 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 
     2821 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 
     2881 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 
     2941 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 
     3001 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 
     3061 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 
     3121 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 
     3181 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 
     3241 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 
     3301 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 
     3361 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 
     3421 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 
     3481 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 
     3541 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 
     3601 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 
     3661 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 
     3721 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 
     3781 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 
     3841 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG 
     3901 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 
     3961 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 
     4021 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 
     4081 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC 
     4141 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA 
     4201 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 
     4261 GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCATTTAA ATTTTTTTGA TTCGGTAATC 
     4321 TCCGAGCAGA AGGAAGAACG AAGGAAGGAG CACAGACTTA GATTGGTATA TATACGCATA 
     4381 TGTGGTGTTG AAGAAACATG AAATTGCCCA GTATTCTTAA CCCAACTGCA CAGAACAAAA 
     4441 ACCTGCAGGA AACGAAGATA AAACATGTCC ACAAAATCAT ATACCAGTAG AGCTGAGACT 
     4501 CATGCAAGTC CGGTTGCATC GAAACTTTTA CGTTTAATGG ATGAAAAGAA GACCAATTTG 
     4561 TGTGCTTCTC TTGACGTTCG TTCGACTGAT GAGCTATTGA AACTTGTTGA AACGTTGGGT 
     4621 CCATACATTT GCCTTTTGAA AACACACGTT GATATCTTGG ATGATTTCAG TTATGAGGGT 
     4681 ACTGTCGTTC CATTGAAAGC ATTGGCAGAG AAATACAAGT TCTTGATATT TGAGGACAGA 
     4741 AAATTCGCCG ATATCGGTAA CACAGTCAAA TTACAATATA CATCGGGCGT TTACCGTATC 
     4801 GCAGAATGGT CTGATATCAC CAACGCCCAC GGGGTTACTG GTGCTGGTAT TGTTGCTGGC 
     4861 TTGAAACAAG GTGCGCAAGA GGTCACCAAA GAACCAAGGG GATTATTGAT GCTTGCTGAA 
     4921 TTGTCTTCCA AGGGTTCTCT AGCACACGGT GAATATACTA AGGGTACCGT TGATATTGCA 
     4981 AAGAGTGATA AAGATTTCGT TATTGGGTTC ATTGCTCAGA ACGATATGGG AGGAAGAGAA 
     5041 GAAGGGTTTG ATTGGCTAAT CATGACCCCA GGTGTAGGTT TAGACGACAA AGGCGATGCA 
     5101 TTGGGTCAGC AGTACAGAAC CGTCGACGAA GTTGTAAGTG GTGGATCAGA TATCATCATT 
     5161 GTTGGCAGAG GACTTTTCGC CAAGGGTAGA GATCCTAAGG TTGAAGGTGA AAGATACAGA 
     5221 AATGCTGGAT GGGAAGCGTA CCAAAAGAGA ATCAGCGCTC CCCATTAATT ATACAGGAAA 
     5281 CTTAATAGAA CAAATCACAT ATTTAATCTA ATAGCCACCT GCATTGGCAC GGTGCAACAC 
     5341 TCACTTCAAC TTCATCTTAC AAAAGATCAC GTGATCTGTT GTATTGGGAT CTCTAGACCT 
     5401 AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTGCTCG AG 
//