LOCUS       In-Vivo\Anneal         11134 bp    DNA     circular     15-Sep-2020
FEATURES             Location/Qualifiers
     primer_bind     complement(10857..10879)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     10979..10998
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     promoter        1659..2325
                     /label=GAP promoter
                     /note=promoter for glyceraldehyde-3-phosphate dehydrogenase
     primer_bind     2195..2218
                     /label=GPDpro-F
                     /note=S. cerevisiae GPD promoter, forward primer
     regulatory      2354..2363
                     /label=vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)
                     /note=vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)
     CDS             2360..6463
                     /label=Cas9
                     /note=generates RNA-guided double strand breaks in DNA
     CDS             6473..6493
                     /label=SV40 NLS
                     /note=nuclear localization signal of SV40 (simian virus40) large T antigen
     terminator      6521..6708
                     /label=ADH1 terminator
                     /note=transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene
     primer_bind     complement(6576..6600)
                     /label=pHybLex-R
                     /note=ADH terminator, reverse primer
     terminator      6826..6845
                     /label=SUP4 terminator
                     /note=transcription terminator for the S. cerevisiae SUP4 tRNA gene
     promoter        complement(6927..7195)
                     /label=SNR52 promoter
                     /note=promoter for the S. cerevisiae small nucleolar RNA gene SNR52
     primer_bind     complement(7226..7246)
                     /label=T3
                     /note=T3 promoter, forward primer
     promoter        complement(7226..7244)
                     /label=T3 promoter
                     /note=promoter for bacteriophage T3 RNA polymerase
     primer_bind     complement(7265..7281)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(7278..7300)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    7289..7305
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(7313..7343)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     primer_bind     complement(7496..7513)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(7667..8255)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(7747..7766)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     CDS             complement(8426..9286)
                     /label=AmpR
                     /note=confers resistance to ampicillin, carbenicillin, andrelated antibiotics
     primer_bind     9049..9068
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     promoter        complement(9287..9391)
                     /label=AmpR promoter
                     /note=promoter
     rep_origin      9418..10760
                     /label=2u ori
                     /note=yeast 2u plasmid origin of replication
     protein_bind    complement(10381..10428)
                     /label=FRT
                     /note=FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011).
     primer_bind     10801..10819
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     CDS             501..1310
                     /label=KanMX4
                     /note=Detected CDS 269aa
     misc_feature    69..102
                     /label=LoxP
                     /note=misc_feature
     misc_feature    1576..1609
                     /label=LoxP
                     /note=misc_feature
     misc_feature    69..102
                     /label=LoxP
                     /note=misc_feature
     misc_feature    1576..1609
                     /label=LoxP
                     /note=misc_feature
     promoter        112..500
                     /label=AgTEF promoter
                     /note=promoter
     terminator      1311..1540
                     /label=AgTEF terminator
                     /note=terminator
     promoter        171..500
                     /label=AgTEF promoter
                     /note=promoter
     terminator      1311..1533
                     /label=AgTEF1 terminator
                     /note=terminator
     rep_origin      7652..8271
                     /label=rep
                     /note=rep_origin
     misc_feature    7650..7744
                     /label=ori
                     /note=/SECDrawAs=Region
     misc_feature    7606..7641
                     /label=Gibson Assembly arm
                     /note=misc_feature
     promoter        complement(7261..7279)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(9328..9356)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
BASE COUNT     3141 a      2574 c      2531 g      2888 t 
ORIGIN
        1 GACTTAGATT GGTATATATA CGCATATGCA GCTGAAGCTT CGTACGCTGC AGGTCGACAA 
       61 CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCTA GAGATCTGTT 
      121 TAGCTTGCCT CGTCCCCGCC GGGTCACCCG GCCAGCGACA TGGAGGCCCA GAATACCCTC 
      181 CTTGACAGTC TTGACGTGCG CAGCTCAGGG GCATGATGTG ACTGTCGCCC GTACATTTAG 
      241 CCCATACATC CCCATGTATA ATCATTTGCA TCCATACATT TTGATGGCCG CACGGCGCGA 
      301 AGCAAAAATT ACGGCTCCTC GCTGCAGACC TGCGAGCAGG GAAACGCTCC CCTCACAGAC 
      361 GCGTTGAATT GTCCCCACGC CGCGCCCCTG TAGAGAAATA TAAAAGGTTA GGATTTGCCA 
      421 CTGAGGTTCT TCTTTCATAT ACTTCCTTTT AAAATCTTGC TAGGATACAG TTCTCACATC 
      481 ACATCCGAAC ATAAACAACC ATGGGTAAGG AAAAGACTCA CGTTTCGAGG CCGCGATTAA 
      541 ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT 
      601 CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC 
      661 ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA 
      721 CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT 
      781 TACTCACCAC TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT 
      841 CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG 
      901 TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA 
      961 TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG 
     1021 AACAAGTCTG GAAAGAAATG CATAAGCTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC 
     1081 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG 
     1141 ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC 
     1201 TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC 
     1261 CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGTACTGA 
     1321 CAATAAAAAG ATTCTTGTTT TCAAGAACTT GTCATTTGTA TAGTTTTTTT ATATTGTAGT 
     1381 TGTTCTATTT TAATCAAATG TTAGCGTGAT TTATATTTTT TTTCGCCTCG ACATCATCTG 
     1441 CCCAGATGCG AAGTTAAGTG CGCAGAAAGT AATATCATGC GTCAATCGTA TGTGAATGCT 
     1501 GGTCGCTATA CTGCTGTCGA TTCGATACTA ACGCCGCCAT CCAGTGTCGA AAACGAGCTC 
     1561 TCGAGAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTGATATC 
     1621 AGATCCACTA GTGGCCTATA CTAGTCAGTT CGAGTTTATC ATTATCAATA CTGCCATTTC 
     1681 AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC TTTATTTAGT CAAAAAATTA 
     1741 GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG GGGGCGGGTT ACACAGAATA 
     1801 TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG CATCCACTAA ATATAATGGA 
     1861 GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC CAGCACCAAA ATATTGTTTT 
     1921 CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC AACTACAGAG AACAGGGGCA 
     1981 CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG CAACCTGCCT GGAGTAAATG 
     2041 ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT TTTCTTACAC CTTCTATTAC 
     2101 CTTCTGCTCT CTCTGATTTG GAAAAAGCTG AAAAAAAAGG TTGAAACCAG TTCCCTGAAA 
     2161 TTATTCCCCT ACTTGACTAA TAAGTATATA AAGACGGTAG GTATTGATTG TAATTCTGTA 
     2221 AATCTATTTC TTAAACTTCT TAAATTCTAC TTTTATAGTT AGTCTTTTTT TTAGTTTTAA 
     2281 AACACCAAGA ACTTAGTTTC GAATAAACAC ACATAAACAA ACAAAAGATC TGATATGGAT 
     2341 CGAATTAGAT CTCGCCACCA TGGACAAGAA GTATTCTATC GGACTGGACA TCGGGACTAA 
     2401 TAGCGTCGGG TGGGCCGTCA TCACTGACGA GTACAAGGTG CCCTCTAAGA AGTTCAAGGT 
     2461 GCTCGGGAAC ACCGACCGGC ATTCCATCAA GAAAAATCTG ATCGGAGCTC TCCTCTTTGA 
     2521 TTCAGGGGAG ACCGCTGAAG CAACCCGCCT CAAGCGGACT GCTAGACGGC GGTACACCAG 
     2581 GAGGAAGAAC CGGATTTGTT ACCTTCAAGA GATATTCTCC AACGAAATGG CAAAGGTCGA 
     2641 CGACAGCTTC TTCCATAGGC TGGAAGAATC ATTCCTCGTG GAAGAGGATA AGAAGCATGA 
     2701 ACGGCATCCC ATCTTCGGTA ATATCGTCGA CGAGGTGGCC TATCACGAGA AATACCCAAC 
     2761 CATCTACCAT CTTCGCAAAA AGCTGGTGGA CTCAACCGAC AAGGCAGACC TCCGGCTTAT 
     2821 CTACCTGGCC CTGGCCCACA TGATTAAGTT CAGAGGCCAC TTCCTGATCG AGGGCGACCT 
     2881 CAATCCTGAC AATAGCGATG TGGATAAACT GTTCATCCAG CTGGTGCAGA CTTACAACCA 
     2941 GCTCTTTGAA GAGAACCCCA TCAATGCAAG CGGAGTCGAT GCCAAGGCCA TTCTGTCAGC 
     3001 CCGGCTGTCA AAGAGCCGCA GACTTGAGAA TCTTATCGCT CAGCTGCCGG GTGAAAAGAA 
     3061 AAATGGACTG TTCGGGAACC TGATTGCTCT TTCACTTGGG CTGACTCCCA ATTTCAAGTC 
     3121 TAATTTCGAC CTGGCAGAGG ATGCCAAGCT GCAACTGTCC AAGGACACCT ATGATGACGA 
     3181 TCTCGACAAC CTCCTGGCCC AGATCGGTGA CCAATACGCC GACCTTTTCC TTGCTGCTAA 
     3241 GAATCTTTCT GACGCCATCC TGCTGTCTGA CATTCTCCGC GTGAACACTG AAATCACCAA 
     3301 GGCCCCTCTT TCAGCTTCAA TGATTAAGCG GTATGATGAG CACCACCAGG ACCTGACCCT 
     3361 GCTTAAGGCA CTCGTCCGGC AGCAGCTTCC GGAGAAGTAC AAGGAAATCT TCTTTGACCA 
     3421 GTCAAAGAAT GGATACGCCG GCTACATCGA CGGAGGTGCC TCCCAAGAGG AATTTTATAA 
     3481 GTTTATCAAA CCTATCCTTG AGAAGATGGA CGGCACCGAA GAGCTCCTCG TGAAACTGAA 
     3541 TCGGGAGGAT CTGCTGCGGA AGCAGCGCAC TTTCGACAAT GGGAGCATTC CCCACCAGAT 
     3601 CCATCTTGGG GAGCTTCACG CCATCCTTCG GCGCCAAGAG GACTTCTACC CCTTTCTTAA 
     3661 GGACAACAGG GAGAAGATTG AGAAAATTCT CACTTTCCGC ATCCCCTACT ACGTGGGACC 
     3721 CCTCGCCAGA GGAAATAGCC GGTTTGCTTG GATGACCAGA AAGTCAGAAG AAACTATCAC 
     3781 TCCCTGGAAC TTCGAAGAGG TGGTGGACAA GGGAGCCAGC GCTCAGTCAT TCATCGAACG 
     3841 GATGACTAAC TTCGATAAGA ACCTCCCCAA TGAGAAGGTC CTGCCGAAAC ATTCCCTGCT 
     3901 CTACGAGTAC TTTACCGTGT ACAACGAGCT GACCAAGGTG AAATATGTCA CCGAAGGGAT 
     3961 GAGGAAGCCC GCATTCCTGT CAGGCGAACA AAAGAAGGCA ATTGTGGACC TTCTGTTCAA 
     4021 GACCAATAGA AAGGTGACCG TGAAGCAGCT GAAGGAGGAC TATTTCAAGA AAATTGAATG 
     4081 CTTCGACTCT GTGGAGATTA GCGGGGTCGA AGATCGGTTC AACGCAAGCC TGGGTACCTA 
     4141 CCATGATCTG CTTAAGATCA TCAAGGACAA GGATTTTCTG GACAATGAGG AGAACGAGGA 
     4201 CATCCTTGAG GACATTGTCC TGACTCTCAC TCTGTTCGAG GACCGGGAAA TGATCGAGGA 
     4261 GAGGCTTAAG ACCTACGCCC ATCTGTTCGA CGATAAAGTG ATGAAGCAAC TTAAACGGAG 
     4321 AAGATATACC GGATGGGGAC GCCTTAGCCG CAAACTCATC AACGGAATCC GGGACAAACA 
     4381 GAGCGGAAAG ACCATTCTTG ATTTCCTTAA GAGCGACGGA TTCGCTAATC GCAACTTCAT 
     4441 GCAACTTATC CATGATGATT CCCTGACCTT TAAGGAGGAC ATCCAGAAGG CCCAAGTGTC 
     4501 TGGACAAGGT GACTCACTGC ACGAGCATAT CGCAAATCTG GCTGGTTCAC CCGCTATTAA 
     4561 GAAGGGTATT CTCCAGACCG TGAAAGTCGT GGACGAGCTG GTCAAGGTGA TGGGTCGCCA 
     4621 TAAACCAGAG AACATTGTCA TCGAGATGGC CAGGGAAAAC CAGACTACCC AGAAGGGACA 
     4681 GAAGAACAGC AGGGAGCGGA TGAAAAGAAT TGAGGAAGGG ATTAAGGAGC TCGGGTCACA 
     4741 GATCCTTAAA GAGCACCCGG TGGAAAACAC CCAGCTTCAG AATGAGAAGC TCTATCTGTA 
     4801 CTACCTTCAA AATGGACGCG ATATGTATGT GGACCAAGAG CTTGATATCA ACAGGCTCTC 
     4861 AGACTACGAC GTGGACCACA TCGTCCCTCA GAGCTTCCTC AAAGACGACT CAATTGACAA 
     4921 TAAGGTGCTG ACTCGCTCAG ACAAGAACCG GGGAAAGTCA GATAACGTGC CCTCAGAGGA 
     4981 AGTCGTGAAA AAGATGAAGA ACTATTGGCG CCAGCTTCTG AACGCAAAGC TAATCACTCA 
     5041 GCGGAAGTTC GACAATCTCA CTAAGGCTGA GAGGGGCGGA CTGAGCGAAC TGGACAAAGC 
     5101 AGGATTCATT AAACGGCAAC TTGTGGAGAC TCGGCAGATT ACTAAACATG TCGCCCAAAT 
     5161 CCTTGACTCA CGCATGAATA CCAAGTACGA CGAAAACGAC AAACTTATCC GCGAGGTGAA 
     5221 GGTGATTACC CTGAAGTCCA AGCTGGTCAG CGATTTCAGA AAGGACTTTC AATTCTACAA 
     5281 AGTGCGGGAG ATCAATAACT ATCATCATGC TCATGACGCA TATCTGAATG CCGTGGTGGG 
     5341 AACCGCCCTA ATCAAGAAGT ACCCAAAGCT GGAAAGCGAG TTCGTGTACG GAGACTACAA 
     5401 GGTCTACGAC GTGCGCAAGA TGATTGCCAA ATCTGAGCAG GAGATCGGAA AGGCCACCGC 
     5461 AAAGTACTTC TTCTACAGCA ACATCATGAA TTTCTTCAAG ACCGAAATCA CCCTTGCAAA 
     5521 CGGTGAGATC CGGAAGAGGC CGCTCATCGA GACTAATGGG GAGACTGGCG AAATCGTGTG 
     5581 GGACAAGGGC AGAGATTTCG CTACCGTGCG CAAAGTGCTT TCTATGCCTC AAGTGAACAT 
     5641 CGTGAAGAAA ACCGAGGTGC AAACCGGAGG CTTTTCTAAG GAATCAATCC TCCCCAAGCG 
     5701 CAACTCCGAC AAGCTCATTG CAAGGAAGAA GGATTGGGAC CCTAAGAAGT ACGGCGGATT 
     5761 CGATTCACCA ACTGTGGCTT ATTCTGTCCT GGTCGTGGCT AAGGTGGAAA AAGGAAAGTC 
     5821 TAAGAAGCTC AAGAGCGTGA AGGAACTGCT GGGTATCACC ATTATGGAGC GCAGCTCCTT 
     5881 CGAGAAGAAC CCAATTGACT TTCTCGAAGC CAAAGGTTAC AAGGAAGTCA AGAAGGACCT 
     5941 TATCATCAAG CTCCCAAAGT ATAGCCTGTT CGAACTGGAG AATGGGCGGA AGCGGATGCT 
     6001 CGCCTCCGCT GGCGAACTTC AGAAGGGTAA TGAGCTGGCT CTCCCCTCCA AGTACGTGAA 
     6061 TTTCCTCTAC CTTGCAAGCC ATTACGAGAA GCTGAAGGGG AGCCCCGAGG ACAACGAGCA 
     6121 AAAGCAACTG TTTGTGGAGC AGCATAAGCA TTATCTGGAC GAGATCATTG AGCAGATTTC 
     6181 CGAGTTTTCT AAACGCGTCA TTCTCGCTGA TGCCAACCTC GATAAAGTCC TTAGCGCATA 
     6241 CAATAAGCAC AGAGACAAAC CAATTCGGGA GCAGGCTGAG AATATCATCC ACCTGTTCAC 
     6301 CCTCACCAAT CTTGGTGCCC CTGCCGCATT CAAGTACTTC GACACCACCA TCGACCGGAA 
     6361 ACGCTATACC TCCACCAAAG AAGTGCTGGA CGCCACCCTC ATCCACCAGA GCATCACCGG 
     6421 ACTTTACGAA ACTCGGATTG ACCTCTCACA GCTCGGAGGG GATGAGGGAG CTCCCAAGAA 
     6481 AAAGCGCAAG GTAGGTAGTT CCGGATCCTA GACTCTCGAG GCGAATTTCT TATGATTTAT 
     6541 GATTTTTATT ATTAAATAAG TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC 
     6601 TTAGGTTTTA AAACGAAAAT TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT 
     6661 TTCTCAGGTA TAGCATGAGG TCGCTCTTAT TGACCACACC TCTACCGGCA TGCCGAGCAA 
     6721 ATGCCTGCAA ATCGCTCCCC ATTTCTCTAG AGCGGCCGTG GTATCGTTTA GATTGGCAAT 
     6781 TACAGTGTCT TAGCTCACAT GCTTATAACT AATTACATGA CTCGAAGACA TAAAAAACAA 
     6841 AAAAAGCACC ACCGACTCGG TGCCACTTTT TCAAGTTGAT AACGGACTAG CCTTATTTTA 
     6901 ACTTGCTATT TAAATGCATC GATTCTGATC ATTTATCTTT CACTGCGGAG AAGTTTCGAA 
     6961 CGCCGAAACA TGCGCACCAA CTTTCACTTC TACAGCGTTT GACCAAAATC TTTTGAACAG 
     7021 AACATTGTAG GGTGTGAAAA AATGCGCACC TTTACCGCTA GCCCAAGAGG GCACTACAAA 
     7081 ATCTAGAGTT GTACTTCAAA CGTACATGTA ATCACCTTGT ATATACTCGA AAGAAAACAT 
     7141 CAAGTTTCTG TATAAATATG AGTGAAAGCA TAATCATACA TTATCTTTTC AAAGATTGTC 
     7201 CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT 
     7261 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAG 
     7321 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGGTAA CTCACATTAA 
     7381 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     7441 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     7501 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7561 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7621 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7681 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7741 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7801 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7861 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7921 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7981 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     8041 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     8101 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     8161 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 
     8221 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     8281 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     8341 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     8401 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8461 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8521 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8581 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8641 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8701 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8761 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8821 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8881 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8941 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     9001 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     9061 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     9121 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     9181 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     9241 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     9301 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     9361 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAC 
     9421 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA 
     9481 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC 
     9541 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGAGA GCGCTAATTT 
     9601 TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AGAGCGCTAT 
     9661 TTTACCAACA AAGAATCTAT ACTTCTTTTT TGTTCTACAA AAATGCATCC CGAGAGCGCT 
     9721 ATTTTTCTAA CAAAGCATCT TAGATTACTT TTTTTCTCCT TTGTGCGCTC TATAATGCAG 
     9781 TCTCTTGATA ACTTTTTGCA CTGTAGGTCC GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT 
     9841 CTATTTTCTC TTCCATAAAA AAAGCCTGAC TCCACTTCCC GCGTTTACTG ATTACTAGCG 
     9901 AAGCTGCGGG TGCATTTTTT CAAGATAAAG GCATCCCCGA TTATATTCTA TACCGATGTG 
     9961 GATTGCGCAT ACTTTGTGAA CAGAAAGTGA TAGCGTTGAT GATTCTTCAT TGGTCAGAAA 
    10021 ATTATGAACG GTTTCTTCTA TTTTGTCTCT ATATACTACG TATAGGAAAT GTTTACATTT 
    10081 TCGTATTGTT TTCGATTCAC TCTATGAATA GTTCTTACTA CAATTTTTTT GTCTAAAGAG 
    10141 TAATACTAGA GATAAACATA AAAAATGTAG AGGTCGAGTT TAGATGCAAG TTCAAGGAGC 
    10201 GAAAGGTGGA TGGGTAGGTT ATATAGGGAT ATAGCACAGA GATATATAGC AAAGAGATAC 
    10261 TTTTGAGCAA TGTTTGTGGA AGCGGTATTC GCAATATTTT AGTAGCTCGT TACAGTCCGG 
    10321 TGCGTTTTTG GTTTTTTGAA AGTGCGTCTT CAGAGCGCTT TTGGTTTTCA AAAGCGCTCT 
    10381 GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAA AGCGTTTCCG 
    10441 AAAACGAGCG CTTCCGAAAA TGCAACGCGA GCTGCGCACA TACAGCTCAC TGTTCACGTC 
    10501 GCACCTATAT CTGCGTGTTG CCTGTATATA TATATACATG AGAAGAACGG CATAGTGCGT 
    10561 GTTTATGCTT AAATGCGTAC TTATATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT 
    10621 ACCTCCTGTG ATATTATCCC ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC 
    10681 TTTAGCTGTT CTATATGCTG CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC 
    10741 ATTTCCTTTG ATATTGGATC ATACTAAGAA ACCATTATTA TCATGACATT AACCTATAAA 
    10801 AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC 
    10861 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 
    10921 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG 
    10981 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATACCACAG CTTTTCAATT CAATTCATCA 
    11041 TTTTTTTTTT ATTCTTTTTT TTGATTTCGG TTTCTTTGAA ATTTTTTTGA TTCGGTAATC 
    11101 TCCGAACAGA AGGAAGAACG AAGGAAGGAG CACA 
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