LOCUS       Exported                4976 bp DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2022 from SnapGene Viewer 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(57..645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(816..1676)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1677..1781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1808..3150
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             3686..4360
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     misc_feature    4560..4724
                     /label=ALD6 (125,40)
     misc_feature    4727..4972
                     /label=ALD6 (1054,1749)
ORIGIN
        1 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
       61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1801 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     1861 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     1921 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     1981 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     2041 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     2101 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     2161 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     2221 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     2281 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     2341 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     2401 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     2461 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     2521 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     2581 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     2641 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     2701 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     2761 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa
     2821 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
     2881 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
     2941 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag
     3001 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc
     3061 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt
     3121 caatgctatc atttcctttg atattggatc atattaagaa accattatta tcatgacatt
     3181 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     3361 taactatgcg gcatcagagc agattgtact gagagtgcac cataaacgac attactatat
     3421 atataatata ggaagcattt aatagacagc atcgtaatat atgtgtactt tgcagttatg
     3481 acgccagatg gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta
     3541 caatcttgat ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag
     3601 aaaaggagag ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat
     3661 taagcacaca aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc
     3721 attggtgaaa gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga
     3781 tgctgacttg ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat
     3841 tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata
     3901 cttggttggc gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta
     3961 cggcattgat atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct
     4021 cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc
     4081 agcttcacag aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg
     4141 tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag
     4201 cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt
     4261 aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc
     4321 taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt
     4381 atttaagtat tgtttgtgca cttgcctatg cggtgtgaaa taccgcacag atgcgtaagg
     4441 agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     4501 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttaattta
     4561 aatgtaataa gaagtttggt aatattcaat tcgaagtgtt cagtctttta cttctcttgt
     4621 tttatagaag aaaaaacatc aagaaacatc tttaacatac acaaacacat actatcagaa
     4681 tacaatgact aagctacact ttgacactgc tgaaccagtc aagaggtgta ccaacctgca
     4741 tttctttccg tcatatacac aaaatacttt catataaact tacttggtct tacgtcataa
     4801 ataaatatgt atacatataa attaaaaaat ttggttttat atttttacaa aaagaatcgt
     4861 ttacttcatt tctccctttt aagcgataca atccatgaaa aaagagaaaa agagagaaca
     4921 ggcttgtgcc ttctttaaaa catcccacac aaaatcatat tgaattgaat ttaaat
//
