LOCUS       pILGFP3AE4              7709 bp    DNA     circular     24-Jan-2019
FEATURES             Location/Qualifiers
     CDS             5979..6707
                     /label=yEGFP
                     /note=Detected CDS 242aa
     misc_feature    6714..6914
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(407..1021)
                     /label=pUC
                     /note=pUC Origin
     CDS             complement(1181..2041)
                     /label=bla
                     /note=Ampicilin Resistance
     misc_feature    5393..5978
                     /label=TEF1P
                     /note=misc_feature
     promoter        2648..3222
                     /label=RPL25 promoter
                     /note=promoter
     CDS             3234..4322
                     /label=KlLEU2
                     /note=/SECDrawAs=Gene
     misc_feature    405..499
                     /label=ori
                     /note=/SECDrawAs=Region
     terminator      4476..5071
                     /label=RPL25 terminator
                     /note=terminator
     rep_origin      5072..5291
                     /label=ARS306
                     /note=rep_origin
     CDS             3234..4319
                     /label=KlLEU2
                     /note=CDS
     CDS             7326..7706
                     /label=RPL25 Arm
                     /note=CDS
     promoter        6921..7325
                     /label=ERG1 promoter
                     /note=promoter
BASE COUNT     2146 a      1617 c      1656 g      2290 t 
ORIGIN
        1 CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
       61 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
      121 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
      181 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
      241 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
      301 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
      361 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
      421 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
      481 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
      541 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
      601 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
      661 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
      721 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
      781 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
      841 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
      901 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG 
      961 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     1021 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     1081 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     1141 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     1201 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     1261 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     1321 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     1381 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     1441 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     1501 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     1561 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     1621 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     1681 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     1741 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     1801 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     1861 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     1921 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     1981 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     2041 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     2101 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     2161 AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC 
     2221 GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 
     2281 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 
     2341 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 
     2401 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 
     2461 GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT 
     2521 CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT 
     2581 TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT 
     2641 TCAGTTTAAA CATGTACTAA TCAGTCTAAC ACCCCGGAGG AGGCTTATCA TTACATATTC 
     2701 TTGTCTCGGC GTTCTTTAAG TTTCTTATCT TGTATGCCCG ATATAGCAAC CTTGTTGGTA 
     2761 CCAATCTAAC GGTTTCCGTA CTTTGCAATG AAGAGATGAG GAGGCATGGG TCACTTATTT 
     2821 AATATGTACG GGTGTTTACA TGGAGTTGCT TTCTTTTTTT GTCTCAGCAG TCATTGTGCG 
     2881 CCAAAAAAAG AGAAAACCGT GAGCCGAAGT CCACGCTCTG GAGTTAGGCT CTCCCATTAC 
     2941 GGAGAGAAGC ATTTCCTCAG CCTGGGAGCC CCGTTGGAAC AGTCAGGCTA AACTGGGCCT 
     3001 TCCTACCCAC TGCTTGCTGT TTCTCACTGG ACGCACAAGG GGATTTTCTT TCTACCTTCG 
     3061 GCTTGCCTCA CTGCGTTGGG GCTTCCCAAT GCAACTTCGT TCGTATGCAT ACAATCTTTT 
     3121 AGATATTATC TTTTAAAATT ATTTTAAAAC AATTTTAAAT GTATCTCATA TGCTTTTCTT 
     3181 CTGCTGTTGA AAAGGCTAAA CAAAGAAGAT CAATAAGATA AAGCGGCCGC AAAATGTCTA 
     3241 AGAATATCGT TGTCCTACCG GGTGATCACG TCGGTAAAGA AGTTACTGAC GAAGCTATTA 
     3301 AGGTCTTGAA TGCCATTGCT GAAGTCCGTC CAGAAATTAA GTTCAATTTC CAACATCACT 
     3361 TGATCGGGGG TGCTGCCATC GATGCCACTG GCACTCCTTT ACCAGATGAA GCTCTAGAAG 
     3421 CCTCTAAGAA AGCCGATGCT GTCTTACTAG GTGCTGTTGG TGGTCCAAAA TGGGGTACGG 
     3481 GCGCAGTTAG ACCAGAACAA GGTCTATTGA AGATCAGAAA GGAATTGGGT CTATACGCCA 
     3541 ACTTGAGACC ATGTAACTTT GCTTCTGATT CTTTACTAGA TCTTTCTCCT TTGAAGCCTG 
     3601 AATATGCAAA GGGTACCGAT TTCGTCGTCG TTAGAGAATT GGTTGGTGGT ATCTACTTTG 
     3661 GTGAAAGAAA AGAAGATGAA GGTGACGGAG TTGCTTGGGA CTCTGAGAAA TACAGTGTTC 
     3721 CTGAAGTTCA AAGAATTACA AGAATGGCTG CTTTCTTGGC ATTGCAACAA AACCCACCAT 
     3781 TACCAATCTG GTCTCTTGAC AAGGCTAACG TGCTTGCCTC TTCCAGATTG TGGAGAAAGA 
     3841 CTGTTGAAGA AACCATCAAG ACTGAGTTCC CACAATTAAC TGTTCAGCAC CAATTGATCG 
     3901 ACTCTGCTGC TATGATTTTG GTTAAATCAC CAACTAAGCT AAACGGTGTT GTTATTACCA 
     3961 ACAACATGTT TGGTGATATT ATCTCCGATG AAGCCTCTGT TATTCCAGGT TCTTTGGGTT 
     4021 TATTACCTTC TGCATCTCTA GCTTCCCTAC CTGACACTAA CAAGGCATTC GGTTTGTACG 
     4081 AACCATGTCA TGGTTCTGCC CCAGATTTAC CAGCAAACAA GGTTAACCCA ATTGCTACCA 
     4141 TCTTATCTGC AGCTATGATG TTGAAGTTAT CCTTGGATTT GGTTGAAGAA GGTAGGGCTC 
     4201 TTGAAGAAGC TGTTAGAAAT GTCTTGGATG CAGGTGTCAG AACCGGTGAC CTTGGTGGTT 
     4261 CTAACTCTAC CACTGAGGTT GGCGATGCTA TCGCCAAGGC TGTCAAGGAA ATCTTGGCTT 
     4321 AAAGAGTCTT TTGTAACGAC CCCGTCTCCA CCAACTTGGT ATGCTTGAAA TCTCAAGGCC 
     4381 ATTACACATT CAGTTATGTG AACGAAAGGT CTTTATTTAA CGTAGCATAA ACTAAATAAT 
     4441 ACAGGTTCCG GTTAGCCTGC GGGCCCAAAT CTAGATCTAA TTGGTTTAAT TAATAAATTT 
     4501 AATATTATTT TTAAATTTTT CTTTAAATAT ACAATAAATC TTTCATAACA TGTTAAATTC 
     4561 ATGATTAAGC GTAAATAAAG TGTAGTGGCA GAGTGCACGG GGTTTCCTGT GCCTTACAAA 
     4621 GTAGGTACCA ATTTGCGTAT TGCAGCGAGG GTTCCGGTTA CTATTTATAA TTACGTGTTA 
     4681 GTGTACTGTG ATTTTATTGA GGCTATAACA AGAAAAGGAT CTGTGAAGGT TTTGAGAGAA 
     4741 TGAGGCGAAA ACACAGGGTT ATTTTCATCA GCAACGCCGC TGTTTCAGCG GCACCCTGCC 
     4801 CTTTTCTTTT CGCAAGATGA GAGTAAAGAG TTGTACATCA GGTAAGAATG TTATTATTTA 
     4861 AATTCGAAGT GATAAATTCT TTTCATGATG AATCACTCGC TTATATGGGG TAGAATATAT 
     4921 ATATATGTGT GTGTGTGTGT GTGTTTGTGT ATGTAGGGAT GGTGCGCGTT TGTTGTGTGA 
     4981 CATTTGCTAC TCATTCTTTT CCTTTTCCTA CGACTGGCTT AACGGGAATA TTATCAATTT 
     5041 GCTGCATTCT TATGCTTCGG TCCGATGCTC AAGCTTAACT TCTTCGTGAG GAAGGAAAGT 
     5101 GTTGTCTCCT ACTTTTTTCA AATTTTCGAA TTGTATTTAT ATTTATTTAG TACTTCTTGA 
     5161 GTTTACATAT CCTTCGTAAA AATGCAACTT TTGTCGAAAA ACACTTCCAA AAAAAAATAA 
     5221 TAATGAATTT ATGAAGCATA CTAACGAGCG AGCACATCGC TGAGGTATCA TTACTTCATG 
     5281 AGATAAATTA ACTCGAGCTC GAGCCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA 
     5341 TATTAAGGGT TGCTCGACCA GAAAGCGACC ACCCAACTTT GGCTGATAAT AGCGTATAAA 
     5401 CAATGCATAC TTTGTACGTT CAAAATACAA TGCAGTAGAT ATATTTATGC ATATTACATA 
     5461 TAATACATAT CACATAGGAA GCAACAGGCG CGTTGGACTT TTAATTTTCG AGGACCGCGA 
     5521 ATCCTTACAT CACACCCAAT CCCCCACAAG TGATCCCCCA CACACCATAG CTTCAAAATG 
     5581 TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC CGTACCACTT 
     5641 CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG GTGTCGTTAA 
     5701 TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT TTTCTTCGTC 
     5761 GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT TTTTTTGATT 
     5821 TTTTTCTCTT TCGATGACCT CCCATTGATA TTTAAGTTAA TAAACGGTCT TCAATTTCTC 
     5881 AAGTTTCAGT TTCATTTTTC TTGTTCTATT ACAACTTTTT TTACTTCTTG CTCATTAGAA 
     5941 AGAAAGCATA GCAATCTAAT CTAAGTTTTA ATTACAAAAT GGGATCCTCT AAAGGTGAAG 
     6001 AATTATTCAC TGGTGTTGTC CCAATTTTGG TTGAATTAGA TGGTGATGTT AATGGTCACA 
     6061 AATTTTCTGT CTCCGGTGAA GGTGAAGGTG ATGCTACTTA CGGTAAATTG ACCTTAAAAT 
     6121 TTATTTGTAC TACTGGTAAA TTGCCAGTTC CATGGCCAAC CTTAGTCACT ACTTTCGGTT 
     6181 ATGGTGTTCA ATGTTTTGCG AGATACCCAG ATCATATGAA ACAACATGAC TTTTTCAAGT 
     6241 CTGCCATGCC AGAAGGTTAT GTTCAAGAAA GAACTATTTT TTTCAAAGAT GACGGTAACT 
     6301 ACAAGACCAG AGCTGAAGTC AAGTTTGAAG GTGATACCTT AGTTAATAGA ATCGAATTAA 
     6361 AAGGTATTGA TTTTAAAGAA GATGGTAACA TTTTAGGTCA CAAATTGGAA TACAACTATA 
     6421 ACTCTCACAA TGTTTACATC ATGGCTGACA AACAAAAGAA TGGTATCAAA GTTAACTTCA 
     6481 AAATTAGACA CAACATTGAA GATGGTTCTG TTCAATTAGC TGACCATTAT CAACAAAATA 
     6541 CTCCAATTGG TGATGGTCCA GTCTTGTTAC CAGACAACCA TTACTTATCC ACTCAATCTG 
     6601 CCTTATCCAA AGATCCAAAC GAAAAGAGAG ACCACATGGT CTTGTTAGAA TTTGTTACTG 
     6661 CTGCTGGTAT TACCCATGGT ATGGATGAAT TGTACAAAAG ATCTTAAACT AGTAGGACTA 
     6721 TTTGCAAAGG GAAGGGATGC TAAGGTAGAG GGTGAACGTT ACAGAAAAGC AGGCTGGGAA 
     6781 GCATATTTGA GAAGATGCGG CCAGCAAAAC TAAAAAACTG TATTATAAGT AAATGCATGT 
     6841 ATACTAAACT CACAAATTAG AGCTTCAATT TAATTATATC AGTTATTACC CGGGAATCTC 
     6901 GGTCGTAATG ATTTGCATGC TGCGATACTG CCGTAGCGGG CCTTCGTATA GCTCGGCCGA 
     6961 GCTCGTACAA AAGGCAAGCA GTGTATCGGA CAGAGCTGAT ATAACACAAT ACGCTCGTAG 
     7021 TCGATGCATG CCGTGGCTGC TCTCGGTCGG GTATAAGTCT TAGACAATAG TCTTACCTCG 
     7081 CATGTATAAT AAATCTTTTG TATTTAATCT ATTATATGTT TCTATGCTTT TTTTTCCTAT 
     7141 TGTTGTTTGC TTTTCCTTTT CCTTATTTCT TTCTAGCTTC TAATTTTCTT TCTTTTTTTT 
     7201 TTTTTTTTCA TTGAAAATTA TATATATATA TATATATCAG AACAATTGTC CAGTATTGAA 
     7261 CAATACAGGT TATTTCGAAC AATTGAAAAA AAAAAATCAC AGAAAAACAT ATCGAGAAAA 
     7321 GGGTCATGGC TCCATCTGGT ATGTGAACTG CAATATTAAT AGCACGAGAA AATTGAGAGG 
     7381 AAGATAGATG GGAACTAGTA GAGTTGATAT TGATGAGATA CGAAAACCAC ACGTAAATAA 
     7441 ACTATCCGAC GACAAGAATA GTGCATTAAG GACTTGATTA AGATATGGTG AGCAACGTAA 
     7501 TTATCGGGCT CAACAGTTTA TTAGCAATCG TTTTGATAGA AGCGTTGATG CTGTGGAAGT 
     7561 TGTTCTTTTT ACTACCAACA GCCATTAACA AATCCATTAG AGTGTTCGTT CGTTTTTCTG 
     7621 CTCAGATTCA AGAAAATTAT TCCATCCTCA TTATACTTTT TTCTTCTATT TCGTGCTCCA 
     7681 CGTCGAGGTA TCAAGGAACA TAGTTTAAA 
//