LOCUS       pILGFP4BG6              7195 bp    DNA     circular     29-Mar-2019
FEATURES             Location/Qualifiers
     CDS             3628..4356
                     /label=yEGFP
                     /note=Detected CDS 242aa
     misc_feature    4363..4563
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(4979..5593)
                     /label=pUC
                     /note=pUC Origin
     CDS             complement(5753..6613)
                     /label=bla
                     /note=Ampicilin Resistance
     misc_feature    3042..3627
                     /label=TEF1P
                     /note=misc_feature
     misc_feature    4977..5071
                     /label=ori
                     /note=/SECDrawAs=Region
     rep_origin      4979..5598
                     /label=rep
                     /note=rep_origin
     misc_feature    4933..4968
                     /label=Gibson Assembly arm
                     /note=misc_feature
     CDS             complement(5753..6683)
                     /label=bla
                     /note=CDS
     rep_origin      4994..5582
                     /label=ori
                     /note=rep_origin
     misc_feature    complement(6842..6864)
                     /label=pGEX_3_primer
                     /note=misc_feature
     misc_feature    complement(7063..11)
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    7177..4
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        7192..13
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(4588..4606)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(4605..4627)
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(6655..6683)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     misc_feature    25..633
                     /label=SEC23 promoter region
                     /note=misc_feature
     CDS             970..1998
                     /label=HphMX
                     /note=CDS
     promoter        640..969
                     /label=AgTEF1 promoter
                     /note=promoter
     terminator      1999..2221
                     /label=AgTEF1 terminator
                     /note=terminator
     terminator      2230..2829
                     /label=SEC23 terminator
                     /note=terminator
     rep_origin      2841..2940
                     /label=ARS1max
                     /note=rep_origin
BASE COUNT     1894 a      1644 c      1639 g      2018 t 
ORIGIN
        1 AACGACGGCC AGTGAATTCA GTTTAAACTC TTCTGCTTCG TTCAGCTGCA GGTTAAAGTA 
       61 GTTGTCCGTG GACACGAGCG TACCTCTATA TTCGGTGCTG TTGAACTTAA GCTTGACGCC 
      121 TACACGATGG TTGACCAGGC CTTTGAGGAA CGGCTTCGGG TTCACCGGCT GCATCGCGCT 
      181 GATATCACTG CTCTCGCTCA TTGTCTTGTT GTGTTGTGAC GTAGTGTGTG GTGTGGTATA 
      241 GTACGCGGTG CTGTGTGTGC TGTGCTGTTT TTGCGTTGCT CATAGAACCG TTGTTTCTTG 
      301 CTCTTACCAT CTCATGTTCA TATATGTTGG GGAAAAGAGG TGGAACAAGA AAAGTTACCC 
      361 GGACGGCAGA GCGGTTGTTT TGCCTGCTTT GCCATAATAC AGGATGGGAT CACACACCCC 
      421 CCAAGCCATG CTACAACTTG GAAGAGTGCA ATTCAAGTTG GTTGTCCTCA GTAGTTTGAG 
      481 AGATTGCGCT AATTTTGTTT TATCACCGTT ATTTACTGTC AGTTGAGATT TGTCGCTTGC 
      541 GACGACTAGC CCGCCGCCTC TCGTTTGTTA CAAAGGAGGA AAGTTCTAGC AAGAATAAAA 
      601 GAAAAACACG TTTGAAAAAA GAAAAAAAGA TACGGGCCCG AATACCCTCC TTGACAGTCT 
      661 TGACGTGCGC AGCTCAGGGG CATGATGTGA CTGTCGCCCG TACATTTAGC CCATACATCC 
      721 CCATGTATAA TCATTTGCAT CCATACATTT TGATGGCCGC ACGGCGCGAA GCAAAAATTA 
      781 CGGCTCCTCG CTGCAGACCT GCGAGCAGGG AAACGCTCCC CTCACAGACG CGTTGAATTG 
      841 TCCCCACGCC GCGCCCCTGT AGAGAAATAT AAAAGGTTAG GATTTGCCAC TGAGGTTCTT 
      901 CTTTCATATA CTTCCTTTTA AAATCTTGCT AGGATACAGT TCTCACATCA CATCCGAACA 
      961 TAAACAACCA TGGGTAAAAA GCCTGAACTC ACCGCGACGT CTGTCGAGAA GTTTCTGATC 
     1021 GAAAAGTTCG ACAGCGTCTC CGACCTGATG CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT 
     1081 TTCAGCTTCG ATGTAGGAGG GCGTGGATAT GTCCTGCGGG TAAATAGCTG CGCCGATGGT 
     1141 TTCTACAAAG ATCGTTATGT TTATCGGCAC TTTGCATCGG CCGCGCTCCC GATTCCGGAA 
     1201 GTGCTTGACA TTGGGGAATT CAGCGAGAGC CTGACCTATT GCATCTCCCG CCGTGCACAG 
     1261 GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG CTGTTCTGCA GCCGGTCGCG 
     1321 GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA CGAGCGGGTT CGGCCCATTC 
     1381 GGACCGCAAG GAATCGGTCA ATACACTACA TGGCGTGATT TCATATGCGC GATTGCTGAT 
     1441 CCCCATGTGT ATCACTGGCA AACTGTGATG GACGACACCG TCAGTGCGTC CGTCGCGCAG 
     1501 GCTCTCGATG AGCTGATGCT TTGGGCCGAG GACTGCCCCG AAGTCCGGCA CCTCGTGCAC 
     1561 GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC GCATAACAGC GGTCATTGAC 
     1621 TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG CCAACATCTT CTTCTGGAGG 
     1681 CCGTGGTTGG CTTGTATGGA GCAGCAGACG CGCTACTTCG AGCGGAGGCA TCCGGAGCTT 
     1741 GCAGGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG GTCTTGACCA ACTCTATCAG 
     1801 AGCTTGGTTG ACGGCAATTT CGATGATGCA GCTTGGGCGC AGGGTCGATG CGACGCAATC 
     1861 GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG CCCGCAGAAG CGCGGCCGTC 
     1921 TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA ACCGACGCCC CAGCACTCGT 
     1981 CCGAGGGCAA AGGAATAATC AGTACTGACA ATAAAAAGAT TCTTGTTTTC AAGAACTTGT 
     2041 CATTTGTATA GTTTTTTTAT ATTGTAGTTG TTCTATTTTA ATCAAATGTT AGCGTGATTT 
     2101 ATATTTTTTT TCGCCTCGAC ATCATCTGCC CAGATGCGAA GTTAAGTGCG CAGAAAGTAA 
     2161 TATCATGCGT CAATCGTATG TGAATGCTGG TCGCTATACT GCTGTCGATT CGATACTAAC 
     2221 GGCGGCCGCG AGCAACGGCT TTCTTTTGTT TTATTTGATC GTTATTAGGC TTTTTGTATA 
     2281 ATATAAGGCC TGTTCAGTCC ATTGCGCGTG CAAGAATTTT CCTTTGGACC TGAACGAAAA 
     2341 GAGTAGAGGA TTTTATATAA TATATTCTCC GTCTTTTTTG TACTGCGTGG CCTTTACTAC 
     2401 GAATATCTGT ATCTAATTTC TTTTCTAACT TTTTTATTAC CATTACTACT ACTACTATTA 
     2461 TTATTATTAT TATTATTATT ATTATTATTA TTATTGTGTA TGACTATAAT GCTTTACTGC 
     2521 CTCCAATTAT GCTTTTTTTA TTATTTTTTT TGGCTGAAAC TACAGTATAG TAGGTATGTA 
     2581 TATATCTAAA ACTCAAAAAG TGGCATGTCT AATGTAATAC CATCAAAAAC AATGAAATTA 
     2641 TTTAATGGAG ATAGCCTTTT ACCTAGAATG TTAATGACAT CCTGCGCTAC AGCACCCCCT 
     2701 ATAATGGCCG CGACAGGAGC AAACTCGATA CCTTTCTGTT TGATAAACTG TTGTATGTAA 
     2761 TCGTCCTTTA CCACTGTTGC TGGTACACCC AGATTTTCAC ACCATACGGC CGCATCTCTT 
     2821 TTCATTTGTA TTTAAATCCA TTTCAAATTT TATGTTTAGT TCGAGATCCT CAGTTTTCGG 
     2881 CGCATAGGAA CCACGTACAT AATAACTAAA CATAAATCTA TAATAAATAA AAAACAACGA 
     2941 TGGGAGCTCG AGCCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT 
     3001 GCTCGACCAG AAAGCGACCA CCCAACTTTG GCTGATAATA GCGTATAAAC AATGCATACT 
     3061 TTGTACGTTC AAAATACAAT GCAGTAGATA TATTTATGCA TATTACATAT AATACATATC 
     3121 ACATAGGAAG CAACAGGCGC GTTGGACTTT TAATTTTCGA GGACCGCGAA TCCTTACATC 
     3181 ACACCCAATC CCCCACAAGT GATCCCCCAC ACACCATAGC TTCAAAATGT TTCTACTCCT 
     3241 TTTTTACTCT TCCAGATTTT CTCGGACTCC GCGCATCGCC GTACCACTTC AAAACACCCA 
     3301 AGCACAGCAT ACTAAATTTC CCCTCTTTCT TCCTCTAGGG TGTCGTTAAT TACCCGTACT 
     3361 AAAGGTTTGG AAAAGAAAAA AGAGACCGCC TCGTTTCTTT TTCTTCGTCG AAAAAGGCAA 
     3421 TAAAAATTTT TATCACGTTT CTTTTTCTTG AAAATTTTTT TTTTTGATTT TTTTCTCTTT 
     3481 CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT CAATTTCTCA AGTTTCAGTT 
     3541 TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC TCATTAGAAA GAAAGCATAG 
     3601 CAATCTAATC TAAGTTTTAA TTACAAAATG GGATCCTCTA AAGGTGAAGA ATTATTCACT 
     3661 GGTGTTGTCC CAATTTTGGT TGAATTAGAT GGTGATGTTA ATGGTCACAA ATTTTCTGTC 
     3721 TCCGGTGAAG GTGAAGGTGA TGCTACTTAC GGTAAATTGA CCTTAAAATT TATTTGTACT 
     3781 ACTGGTAAAT TGCCAGTTCC ATGGCCAACC TTAGTCACTA CTTTCGGTTA TGGTGTTCAA 
     3841 TGTTTTGCGA GATACCCAGA TCATATGAAA CAACATGACT TTTTCAAGTC TGCCATGCCA 
     3901 GAAGGTTATG TTCAAGAAAG AACTATTTTT TTCAAAGATG ACGGTAACTA CAAGACCAGA 
     3961 GCTGAAGTCA AGTTTGAAGG TGATACCTTA GTTAATAGAA TCGAATTAAA AGGTATTGAT 
     4021 TTTAAAGAAG ATGGTAACAT TTTAGGTCAC AAATTGGAAT ACAACTATAA CTCTCACAAT 
     4081 GTTTACATCA TGGCTGACAA ACAAAAGAAT GGTATCAAAG TTAACTTCAA AATTAGACAC 
     4141 AACATTGAAG ATGGTTCTGT TCAATTAGCT GACCATTATC AACAAAATAC TCCAATTGGT 
     4201 GATGGTCCAG TCTTGTTACC AGACAACCAT TACTTATCCA CTCAATCTGC CTTATCCAAA 
     4261 GATCCAAACG AAAAGAGAGA CCACATGGTC TTGTTAGAAT TTGTTACTGC TGCTGGTATT 
     4321 ACCCATGGTA TGGATGAATT GTACAAAAGA TCTTAAACTA GTAGGACTAT TTGCAAAGGG 
     4381 AAGGGATGCT AAGGTAGAGG GTGAACGTTA CAGAAAAGCA GGCTGGGAAG CATATTTGAG 
     4441 AAGATGCGGC CAGCAAAACT AAAAAACTGT ATTATAAGTA AATGCATGTA TACTAAACTC 
     4501 ACAAATTAGA GCTTCAATTT AATTATATCA GTTATTACCC GGGAATCTCG GTCGTAATGA 
     4561 TTTAAATGCA TGCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     4621 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     4681 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     4741 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     4801 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     4861 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     4921 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     4981 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     5041 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     5101 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     5161 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     5221 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     5281 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     5341 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     5401 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 
     5461 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     5521 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     5581 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     5641 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     5701 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     5761 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     5821 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     5881 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     5941 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     6001 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     6061 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     6121 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     6181 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     6241 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     6301 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     6361 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     6421 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     6481 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     6541 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     6601 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     6661 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     6721 CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT 
     6781 ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 
     6841 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 
     6901 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 
     6961 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 
     7021 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT 
     7081 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG 
     7141 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAA 
//