LOCUS       Exported               18903 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     LOCUS EU530625 6355 bp DNA circular SYN 30-APR-2008 DEFINITION 
            Cloning vector pWSTK6, complete sequence. ACCESSION EU530625 VERSION
            EU530625.1 GI:186703054 KEYWORDS . SOURCE Cloning vector pWSTK6 
            ORGANISM Cloning vector pWSTK6 other sequences; artificial 
            sequences; vectors. REFERENCE 1 (bases 1 to 6355) AUTHORS Wu,S., 
            Ying,G., Wu,Q. and Capecchi,M.R. TITLE A protocol for constructing 
            gene targeting vectors: generating knockout mice for the cadherin 
            family and beyond JOURNAL Nat Protoc 3 (6), 1056-1076 (2008) PUBMED 
            18546598 REFERENCE 2 (bases 1 to 6355) AUTHORS Wu,S. and 
            Capecchi,M.R. TITLE Direct Submission JOURNAL Submitted 
            (29-FEB-2008) Howard Hughes Medical Institute, University of Utah, 
            15N 2030E, Salt Lake City, UT 84112, USA
FEATURES             Location/Qualifiers
     source          1..18903
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..830)
                     /direction=LEFT
                     /label=p15A origin of replication
                     /note="p15A origin of replication"
     gene            complement(975..1835)
                     /label=bla
     misc_feature    2895..2915
                     /label=Untitled
     misc_feature    complement(2928..4505)
                     /label=PGK-DTA-bpA
                     /note="PGK-DTA-bpA"
     misc_feature    complement(2928..3209)
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    complement(3326..3982)
                     /label=DTA
                     /note="DTA"
     misc_feature    3627..3646
                     /label=Untitled
     misc_feature    complement(3627..3646)
                     /label=Untitled
     misc_feature    3912..3931
                     /label=Untitled
     misc_feature    complement(3992..4505)
                     /label=PGK
                     /note="PGK"
     misc_feature    complement(4167..4186)
                     /label=Untitled
     misc_feature    complement(4482..4505)
                     /label=5' of pPGK
                     /note="5' of pPGK"
     misc_feature    4509..4604
                     /label=Untitled
     misc_feature    complement(4509..4524)
                     /label=HN365
                     /note="HN365"
     misc_feature    complement(4525..9070)
                     /label=3' arm
                     /note="3' arm"
     misc_feature    complement(4539..4558)
                     /label=R26-3ret-for
                     /note="R26-3ret-for"
     repeat_region   5000..5146
                     /label=B1_Mm
     repeat_region   complement(6012..6260)
                     /label=B4
     repeat_region   6318..6419
                     /label=PB1D9
     repeat_region   complement(6718..6739)
                     /label=AT_rich
     repeat_region   complement(7053..7197)
                     /label=B1_Mus1
     repeat_region   complement(7463..7573)
                     /label=MER3
     repeat_region   complement(7617..7784)
                     /label=B2_Mm2
     misc_feature    8840..9070
                     /label=R26-inv-5arm
                     /note="R26-inv-5arm"
     misc_feature    8840..8859
                     /label=R26-inv-5arm-for
                     /note="R26-inv-5arm-for"
     misc_feature    complement(8991..9070)
                     /label=Recombineering 5' arm
                     /note="Recombineering 5' arm"
     misc_feature    complement(9050..9070)
                     /label=R26-inv-5arm-rev
                     /note="R26-inv-5arm-rev"
     misc_feature    9071..9072
                     /label=EM7-kanR cassette
                     /note="EM7-kanR cassette"
     misc_feature    9098..9120
                     /label=Untitled
     misc_feature    complement(9098..9100)
                     /label=Untitled
     misc_feature    9101..9127
                     /label=Untitled
     gene            9129..9162
                     /label=FRT
                     /note="FRT"
     gene            9220..9734
                     /label=PGK promoter
                     /note="PGK promoter"
     misc_feature    complement(9558..9572)
                     /label=Untitled
     misc_feature    complement(9620..9636)
                     /label=Untitled
     gene            9737..9802
                     /label=EM7 promoter
                     /note="EM7 promoter"
     CDS             9803..10603
                     /label=Neo
                     /note="Neo"
     gene            10620..10922
                     /label=bGHpA
                     /note="bGHpA"
     gene            10923..10956
                     /label=FRT
                     /note="FRT"
     enhancer        10992..11369
                     /label=hCMV
                     /note="hCMV"
     promoter        11375..12716
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     intron          11744..12668
                     /note="intron"
     misc_feature    12677..12696
                     /label=Untitled
     gene            complement(12723..12756)
                     /label=loxP
                     /note="loxP"
     misc_feature    12757..12819
                     /label=Spacer
                     /note="Spacer"
     misc_feature    complement(12820..12853)
                     /label=loxP5171
                     /note="loxP5171"
     misc_feature    12854..12859
                     /label=Kozak ATG
                     /note="Kozak ATG"
     misc_feature    12854..12859
                     /label=Nco1 site?
                     /note="Nco1 site?"
     CDS             complement(12857..12862)
                     /label=EGFP
                     /note="EGFP"
     misc_feature    complement(12863..13579)
                     /label=sfGFP
                     /note="sfGFP"
     misc_feature    12869..12890
                     /label=Untitled
     misc_feature    12875
                     /label=can be mutated to a stop codon
                     /note="can be mutated to a stop codon"
     misc_feature    12914
                     /label=Untitled
     misc_feature    12920
                     /label=can be mutated to a stop codon
                     /note="can be mutated to a stop codon"
     misc_feature    12923
                     /label=can be mutated to a stop codon
                     /note="can be mutated to a stop codon"
     misc_feature    12962
                     /label=Untitled
     misc_feature    12980
                     /label=Untitled
     misc_feature    12998
                     /label=Untitled
     misc_feature    13001
                     /label=Untitled
     misc_feature    13049
                     /label=Untitled
     misc_feature    13160
                     /label=Untitled
     misc_feature    13172
                     /label=Untitled
     misc_feature    13196
                     /label=Untitled
     misc_feature    13208
                     /label=Untitled
     misc_feature    13391
                     /label=Untitled
     misc_feature    13424
                     /label=Untitled
     misc_feature    13451
                     /label=Untitled
     misc_feature    13457
                     /label=Untitled
     misc_feature    13529
                     /label=Untitled
     misc_feature    13538
                     /label=Untitled
     misc_feature    complement(13556..13579)
                     /label=Untitled
     misc_feature    13562
                     /label=Untitled
     misc_feature    complement(13580..13585)
                     /label=Untitled
     misc_feature    13580..13582
                     /label=Nhe1
                     /note="Nhe1"
     misc_feature    13582..13587
                     /label=Nco1
                     /note="Nco1"
     CDS             complement(13586..13588)
                     /label=EGFP
                     /note="EGFP"
     gene            13589..13622
                     /label=loxP
                     /note="loxP"
     misc_feature    13623..13686
                     /label=Spacer
                     /note="Spacer"
     misc_feature    13623
                     /label=HN255
                     /note="HN255"
     misc_feature    13635..13654
                     /label=HN721
                     /note="HN721"
     misc_feature    13650
                     /label=BamH1
                     /note="BamH1"
     misc_feature    13651..13656
                     /label=EcoR5
                     /note="EcoR5"
     gene            13688..13721
                     /label=loxP5171
                     /note="loxP5171"
     misc_feature    13722..13753
                     /label=rox
                     /note="rox"
     misc_feature    13754..13764
                     /label=3x STOP
                     /note="3x STOP"
     misc_feature    13765
                     /label=WPRE
     misc_feature    13771..14356
                     /label=WPRE
     misc_feature    complement(14339..14367)
                     /label=HN260
                     /note="HN260"
     misc_feature    14353..14380
                     /label=HN261
                     /note="HN261"
     misc_feature    14357..14362
                     /label=Untitled
     gene            14363..14664
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    14476..14481
                     /label=Untitled
     misc_feature    14492..14494
                     /label=cleavage site
                     /note="cleavage site"
     misc_feature    complement(14495..14497)
                     /label=Untitled
     misc_feature    14497..14499
                     /label=cleavage site
                     /note="cleavage site"
     misc_feature    complement(14500..14502)
                     /label=Untitled
     misc_feature    14508..14523
                     /label=necessary for polyadenylation
                     /note="necessary for polyadenylation"
     misc_feature    14670..14897
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    complement(14683..14685)
                     /label=probably Taq error
                     /note="probably Taq error"
     misc_feature    14903..15130
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    15136..15363
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    complement(15248..15253)
                     /label=Untitled
     misc_feature    15268..15273
                     /label=Untitled
     misc_feature    15297..15302
                     /label=Untitled
     misc_feature    complement(15340..15378)
                     /label=HN262
                     /note="HN262"
     misc_feature    15364..15417
                     /label=HN263
                     /note="HN263"
     misc_feature    15369
                     /label=Age1
                     /note="Age1"
     misc_feature    15375..15406
                     /label=rox
                     /note="rox"
     misc_feature    15407..15460
                     /label=T2A
                     /note="T2A"
     misc_feature    15461..16891
                     /label=tdTomato
                     /note="tdTomato"
     misc_feature    16124..16207
                     /label=linker
                     /note="linker"
     misc_feature    16856..16874
                     /label=HN722
                     /note="HN722"
     misc_feature    16892..16902
                     /label=3x STOP
                     /note="3x STOP"
     misc_feature    16903..17492
                     /label=WPRE
     misc_feature    17493..17794
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    17607..17612
                     /label=Untitled
     misc_feature    17623..17625
                     /label=cleavage site
                     /note="cleavage site"
     misc_feature    complement(17626..17628)
                     /label=Untitled
     misc_feature    17628..17630
                     /label=cleavage site
                     /note="cleavage site"
     misc_feature    complement(17631..17633)
                     /label=Untitled
     misc_feature    17639..17654
                     /label=necessary for polyadenylation
                     /note="necessary for polyadenylation"
     misc_feature    complement(17801..18877)
                     /label=5' arm
                     /note="5' arm"
     misc_feature    17801..17880
                     /label=Recombineering 3' arm
                     /note="Recombineering 3' arm"
     misc_feature    17915..17941
                     /label=ZFN
                     /note="ZFN"
     misc_feature    complement(17995..18014)
                     /label=R26-inv-3arm-rev
                     /note="R26-inv-3arm-rev"
     misc_feature    18614..18633
                     /label=R26-5ret-rev
                     /note="R26-5ret-rev"
     misc_feature    18798..18877
                     /label=Untitled
     misc_feature    complement(18858..18877)
                     /label=R26-5ret-for
                     /note="R26-5ret-for"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttcccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
      421 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
      481 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
      541 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
      601 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
      661 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
      721 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
      781 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaaggg gtctgacgct
      841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1261 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1561 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     1981 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     2041 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2101 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2161 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     2221 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     2281 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2341 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     2401 cagtcacgac gttgtaaaac gacggccagt gccaaaacga cggccagtgc caagctttcg
     2461 cgagctcgac ctgcagccaa gcttgggtcg tggacctgac cccacgcaac gcccaaaata
     2521 ataaccccca cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc
     2581 cagtggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     2641 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     2701 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     2761 acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
     2821 ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccgggcaa acgtgcgcgc
     2881 aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg agccccagct
     2941 ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc
     3001 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
     3061 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
     3121 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
     3181 cacagtcgag gctgatcagc gagctctagg atctgcattc caccactgct cccattcatc
     3241 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     3301 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     3361 ttatgtcaca ccacagaagt aaggttcctt cacaaagaga tcgcctgaca cgatttcctg
     3421 cacaggcttg agccatatac tcatacatcg catcttggcc acgttttcca cgggtttcaa
     3481 aattaatctc aagttctacg cttaacgctt tcgcctgttc ccagttatta atatattcaa
     3541 cgctagaact cccctcagcg aagggaaggc tgagcactac acgcgaagca ccatcaccga
     3601 accttttgat aaactcttcc gttccgactt gctccatcaa cggttcagtg agacttaaac
     3661 ctaactcttt cttaatagtt tcggcattat ccacttttag tgcgagaacc ttcgtcagtc
     3721 ctggatacgt cactttgacc acgcctccag cttttccaga gagcgggttt tcattatcta
     3781 cagagtatcc cgcagcgtcg tatttattgt cggtactata aaaccctttc caatcatcgt
     3841 cataatttcc ttgtgtacca gattttggct tttgtatacc tttttgaatg gaatctacat
     3901 aaccaggttt agtcccgtgg tacgaagaaa agttttccat cacaaaagat ttagaagaat
     3961 caacaacatc atcaggatcc atggcgagga cctgcaggtc gaaaggcccg gagatgagga
     4021 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
     4081 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
     4141 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
     4201 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg
     4261 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
     4321 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg
     4381 cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc
     4441 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
     4501 gtagaattcg ccaatgacaa gacggagcca tggcaatgtt caagcaggac caaatgtggt
     4561 gcagtgttga gggcaatctg ggaaggttcc ttaagaagtt atgttctgag accattctca
     4621 gtggctcaac aacacttggt caaaaatttt aattctcccc tcagagaaat ggagtagtta
     4681 ctccactttc aagttcctta taagcttacc atcaacctta tagtacactc tagatgtcct
     4741 gaaatatttc tatcagaaca aggtagtata aagctggtag gtatacaaaa cgctagacta
     4801 gtttctatcc ctgaccctta atctgctagt atatccgtag gaagttgctt aagtgccact
     4861 agtaccaaca gcctctgtta gagtgaacag tagattttaa tgaagggcca ataaagacca
     4921 cttaagacta ctgactgaat taaaaatcca cccagtggag ttagtattgg ttatagttat
     4981 ataacaaaat atccaactta gccaggcgtg gtggtgcacg cctttaatcc cggcactcag
     5041 gaggcagagg caggcggatt tctgagttcg aggccagcct ggtctacaaa gtgagttcca
     5101 ggacagccag ggctatacag agaaaccctg tctcgaaaac ccaaaaaaaa aaaaaaaaag
     5161 gaaaaaaaat atggcttgaa aacatgctat tgttgtcaaa aggagatcca aacaagtatt
     5221 tctttgaaca aaaattatgt agaaaacagg gtgataaaga acaagggctt tttaaaaaag
     5281 gccagcatta tttgagatat acaagtgact ccaattcaag cctaaaggcc actcaatgct
     5341 cactaacagt gctaatcaga atcaattcct tagcccagtc aaaaatagtc ctgaatatct
     5401 tctggataaa catgtcctcc aacatataaa ctagtataca ctaacaaaac gtctcaactt
     5461 caaggtgaaa tgcttgactc ctagacttgt gacccagcaa agtgctctat aggtagggtt
     5521 actaggtcag agagtcttgc ctgcaaacca caattataca attccaccaa atgtaagaca
     5581 tgtaaaaatg acttatttag ctcaggtttt gaaaattaaa aatcaaaccc acaaagacct
     5641 aactttcaca ttaagtacaa atgtttaaca tatataacat gttttgcctt gatatatgaa
     5701 atcatatgac atcacctgac aggaaactta agtttatttg aacaacaatc agcctaaggt
     5761 aggcctagca catgatcagg atagtgcagg gaaacccaaa gaagtgcttc tgagtataaa
     5821 ccaagagacc tcgaaatagc agctttgttc tgtatctcat gggctttaac tatcacttga
     5881 aaacaatttc acaaatcaca aaaccaacat ttgtttagtt tccaactctg cctattcacc
     5941 ggagaaatcc atgtcccaat ttgatggggg aaaacttgaa tgaaatatta tttggaatat
     6001 tttaatatcc ctgtgtatat gcagatggtt taaagacagg taaccttaac tgtagtttct
     6061 tggttgtctg ccttgttttt tgaaatatgc tctcaccagg agcctgccaa gtaactactc
     6121 ttgtgtgctc agcaagtcct agggatccct taagcatgct ctaacaggcc tggctttttt
     6181 ttttaaaaag taggttgcgg tgataataat tcaagttttc atcacttgaa acacatttta
     6241 ccaactatca cccaagctcc cttagtgtgt cccctataaa agaatctgac ctgcaagttc
     6301 caaaagtatt actacatttt aaccttagta ttcaggagac aaaagcaggc agaactttag
     6361 tctacatatc ttgttgcaga tcaggcagag atacatagtg agaccctgcc tcaggaaaag
     6421 ctatcatttt tatattcaca gtaagcaaat taacattaaa agtcagaaat tcttaatttg
     6481 actttggctg tgaagaattt ggattctctg gatttgaaac tagaaaatct gattaagcga
     6541 tgcaacagtt tctgtatact aaaactctaa gacccttggt tctaaagata ccacatttaa
     6601 aaaaaaagtt aagtataaga caaaaagcct taatagctct tttatagggg agggactcat
     6661 ttaatattag tccacctcac tcctcataac tattttatga ggtttattat ataacccatt
     6721 ttttaaaaaa aaaattaaag gacaatttta gtgtttgaaa gatttcccaa ccccaccctg
     6781 gaaatcaggc tgcaaatctc agcagctgcc cttagaaggc aaaaagccat gccatttaag
     6841 ccatgggaag ttagtagcaa acaagagacc acatggcagg aaggaggctt taaagaaagc
     6901 ccacagtgtc aaaagaccca accaacagca gagacagaaa aaataatacc caaatctcat
     6961 cagaaaggta gacggattta gccacatcca tagtggctca ttagggaatg cttcaattat
     7021 aattttatga aatctcagag cccccctccc caacagggtt tctgtgtgta ttcctggcta
     7081 tcctagatag aacttgatgt gtagaccagg ctgggctcaa actcagagat ctgcctgtct
     7141 ctgcctccag agtgctggga ttaaaggcat aaaggcaagc accaccactg gctggctaaa
     7201 ctctggccct acatttttga aatccaaaat tatcattact tgctttcatg tttagtaatg
     7261 gctgcagact tagctttcag ctttgtatag atgaagcaca aaagttaagt aaagctagca
     7321 gtttaaacaa ataggatact agaagttcaa tttttatttc tttattgctt agtgtaagag
     7381 aaaagacatt aaagtctgca ttggtcttct gtatcccaca agtctgcagt tatggctcct
     7441 ctgtccacag ttacacttca aataaaaatt agttccttgg taacatttcc agtgcttaaa
     7501 gagtaatttg attattggct accatattgg aacaaacaca aagtatttca ttacttcagt
     7561 aaattctact ggagacagta ttctctttaa aaaaaaaaaa aagaagaaga aggaggattt
     7621 atttatttaa tgtgaataca cttgtggtct tcagacacac cagaagaggg catcagatcc
     7681 cattacagat ggttgtgagc caccatgtgg ttgctgggaa ttgaactcag gacttctgga
     7741 agagcagtct gtgctcttga actccgagcc acctctccag cccctggata gtattattat
     7801 agagcacaag cacacacaac acaacaacct aaaaaccaag cttcacaaat acatttgttg
     7861 cttaatcatt ttacttgtgt ttggctacaa gtcaagcaaa attataggtc ctgaagaagc
     7921 ttggcaaaat cacatttaga ccagcaataa cgtgtagaat gccatgagtc aagccagtcc
     7981 aagagaaagc actgcttaaa tagaaaaaat acaagctcac aagaccttag gtcaggaaag
     8041 acaacaaaca cctgaacttt gcattccaaa aggaaccacc ttttacagat gtgtacaatg
     8101 acaaaatttt agtaagcagt aatcaatacc atgtggctca ataatgaaat atacctttta
     8161 atgtcttaaa atatcagcct ggcaatatgt aagatacatc aggtaatatt gggggaggag
     8221 acatccacct ggaaaccatt aatggttaat agctcagttt ataaatggag aaaaaggaga
     8281 gaggcattca tgggagtgga aagttaagct ttaggttgga ttctcaatac atctattgtt
     8341 gtaaaactcc ctggactgag aataggccca aatgtggaac accacctgac gggagaggtg
     8401 atagacactg aaaattaagg atcaaggcaa aggatcaaca aaaagtgtac taaggagtta
     8461 taaaagaact gcagtgttga ggccccagct acagcctcga tttgtggtgt atgtaactaa
     8521 tctgtctggt ttcatgagtc atcagacttc taagatcagg aaagggaaaa tgccaatgct
     8581 ctgtctaggg gttggataag ccagtataat aaatgaaaat ggggctaaaa tgagtgttct
     8641 aaaatacctt ttgataaggc tgcagaagga gcgggagaaa tggatatgaa gtactgggct
     8701 ctttaaaaat gattaaaatt ctgcttacat agtctaactc gcgacactgt aatttcatac
     8761 tgtagtaagg atctcaagca ggagagtata aaactcgggt gagcatgtct ttaatctacc
     8821 tcgatggaaa atactccgag gcggatcaca agcaataata acctgtagtt ttgctgcata
     8881 aaaccccaga tgactaccta tcctcccatt ttccttattt gcccctatta aaaaacttcc
     8941 cgacaaaacc gaaaatctgt gggaagtctt gtccctccaa ttttacacct gttcaattcc
     9001 cctgcaggac aacgcccaca caccaggtta gcctttaagc ctgcccagaa gactcccgcc
     9061 catcttctag ggcgcgccga tcttcgaaca tgttaattaa ggtatcgata agcttgatat
     9121 cgaattccga agttcctatt ctctagaaag tataggaact tcaggtctga agaggagttt
     9181 acgtccagcc aagctagctt ggctgcaggt cgtcgaaatt ctaccgggta ggggaggcgc
     9241 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     9301 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     9361 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     9421 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     9481 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     9541 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     9601 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     9661 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     9721 tttcgacctg cagcctgttg acaattaatc atcggcatag tatatcggca tagtataata
     9781 cgacaaggtg aggaactaaa ccatgggatc ggccattgaa caagatggat tgcacgcagg
     9841 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     9901 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     9961 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    10021 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    10081 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10141 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10201 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10261 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10321 gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga
    10381 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10441 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10501 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10561 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg atcaattctc
    10621 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    10681 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    10741 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    10801 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    10861 ctctatggct tctgaggcgg aaagaaccag ctggggctcg actagagctt gcggaaccct
    10921 tcgaagttcc tattctctag aaagtatagg aacttcatca gtcaggtaca taatggatcc
    10981 ggcgcgccgt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    11041 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    11101 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    11161 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    11221 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    11281 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11341 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
    11401 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
    11461 ttgtgcagcg atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg
    11521 cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct
    11581 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
    11641 gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc
    11701 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
    11761 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
    11821 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
    11881 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
    11941 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
    12001 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
    12061 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
    12121 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
    12181 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
    12241 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc
    12301 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
    12361 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
    12421 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
    12481 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
    12541 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
    12601 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
    12661 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
    12721 tcataacttc gtatagcata cattatacga agttattggg ttgacggtga cgcttggcag
    12781 ttcggttcta tcgttaaata ctggtcttct tacttcgaca taacttcgta tagtacacat
    12841 tatacgaagt tataccatgg ctggtaccct tgtacagctc gtccatgccg tgggtgatgc
    12901 cagcggcggt cacaaattcc agcagcacca tgtggtccct cttctcgttg gggtccttgc
    12961 tcagcacgct ctgggtgctc aggtagtggt tgtcaggcag cagcacaggg ccgtcgccga
    13021 taggggtgtt ctgctggtag tggtcggcca gctgcacgct gccatcttcc acgttgtgcc
    13081 tgatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgatg tacacgttgt
    13141 ggctgttgaa gttgtactcc agcttgtggc ccaggatgtt gccgtcctct ttgaagtcga
    13201 tgcccttcag ctcgattctg ttcacgaggg tgtcgccctc gaacttcact tcggctctgg
    13261 tcttgtaggt gccgtcgtcc ttgaagctga tggttctttc ctgcacgtag ccctcgggca
    13321 tggcgctctt gaagaagtcg tgcctcttca tatggtcggg gtatctgctg aagcactgca
    13381 cgccgtaggt cagtgtggtc acgagggtag gccaaggcac gggcagcttg ccggtggtgc
    13441 agatgaactt cagggtcagc tttccgtttg tggcgtcgcc ttcgccctct ccccgcacag
    13501 agaacttgtg gccgttcacg tcgccatcca gttccaccag gatgggcacc acgccggtga
    13561 acagttcctc gcccttgcta gccatggtat aacttcgtat aatgtatgct atacgaagtt
    13621 atatccaagt acgaggacca gtgcttcacc gatatccccc aactgccagg cctccccctc
    13681 cacccatata acttcgtata atgtgtacta tacgaagtta ttaactttaa ataatgccaa
    13741 ttatttaaag ttataactag ataaggatcc aacctctgga ttacaaaatt tgtgaaagat
    13801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
    13861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
    13921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
    13981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
    14041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
    14101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
    14161 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
    14221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
    14281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
    14341 gggccgcctc cccgcatccg gatagagctc gctgatcagc ctcgactgtg ccttctagtt
    14401 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
    14461 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    14521 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    14581 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
    14641 cgactagagc ttgcggaacc cttagctaga atcataatca gccttaccac atttgtagag
    14701 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    14761 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    14821 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    14881 ctcatcaatg tatcttaact agaatcataa tcagccatac cacatttgta gaggttttac
    14941 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    15001 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    15061 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    15121 atgtatctta actagaatca taatcagcca taccacattt gtagaggttt tacttgcttt
    15181 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    15241 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    15301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    15361 ttaactagac cggataactt taaataatgc caattattta aagttagagg gcagaggaag
    15421 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atggtgagca agggcgagga
    15481 ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
    15541 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
    15601 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
    15661 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
    15721 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
    15781 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
    15841 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
    15901 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
    15961 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
    16021 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
    16081 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg
    16141 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa
    16201 catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
    16261 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
    16321 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
    16381 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
    16441 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
    16501 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
    16561 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
    16621 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
    16681 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
    16741 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
    16801 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
    16861 cctgtacggc atggacgagc tgtacaagta ataactagat aaaagcttcc tctggattac
    16921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    16981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    17041 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    17101 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    17161 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    17221 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    17281 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    17341 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    17401 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    17461 agtcggatct ccctttgggc cgcctccccg cactagagct cgctgatcag cctcgactgt
    17521 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    17581 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    17641 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    17701 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    17761 cagctggggc tcgactagag cttgcggaac ccttaattaa aaagactgga gttgcagatc
    17821 acgagggaag agggggaagg gattctccca ggcccagggc ggtcctcaga agccaggagg
    17881 cagcagagaa ctcccagaaa ggtattgcaa cactcccctc ccccctccgg agaagggtgc
    17941 ggccttctcc ccgcctactc cactgcagct cccttactga taacaactca gagcgacttt
    18001 gggagagcaa gtgcttcctg cctccaaaac agcccaactg agccctcgct ccttccctcc
    18061 actccccgga gtgcgcgatg gaggtctggc tcagcacgcc cctcttgagg caactcaagt
    18121 cggaaacgtg cttgcacccg ccccgcagcc gctcagccct actgcccgtc cccgccccca
    18181 gcgcgcgctt cctgccacgt tgcgcagggg cgcgcggcca gactctgcgg cgcggggccg
    18241 aggggagggc cggaacctgg gagcgcctcc tcgccgcccc cgctggccgg cggatggact
    18301 caacttgcac gaacacgagc caatggcaag ggccagtttt ctgggccccg agagccaatc
    18361 agacgacgag gcccggccgg cggcgggtaa aacgactccc ccagaggaag gggagggtgg
    18421 gcggccgctc gcgcgcgagc tactttcgct gaccctccct cccctccccc gccccgccag
    18481 aggccgaccg cgcccgcacg tccagctcgc ctcaccccac ctacctcccg ccccacccag
    18541 tgggcagagc gaggctgccg gcggctgcgc actccggctg ccgttaactg acaggcgcct
    18601 tacgccaacc aaaacacgcc atttgtgttt tcacacacgg cgggaggaaa agaagccaat
    18661 cagcgacgag acgtcggccg gaagcgctcc tccgctgccc cccccccccc gagccatggc
    18721 cgcgtccggt ggagactttt ccgctccctt ctccctcccc ctccggttgc tgcagggcgg
    18781 accgcattcc tgcccaccac ccgcttgccc cttccagcgt cacgactcgt acccggctgt
    18841 ctcacagaac ggctccacca cgctcggagg gcctgccatt taaatatttc gataagccag
    18901 gtt
//
