LOCUS       Exported                2709 bp DNA     circular SYN 04-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..60
                     /label=Pr promoter
     RBS             70..81
     CDS             88..807
                     /codon_start=1
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      819..905
                     /label=rrnB T1 terminator
     primer_bind     983..1002
                     /label=Primer 1
     rep_origin      complement(1075..1620)
                     /direction=LEFT
                     /label=p15A ori
     terminator      1734..1828
                     /label=lambda t0 terminator
     CDS             complement(1849..2508)
                     /codon_start=1
                     /label=CmR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2509..2611)
                     /label=cat promoter
ORIGIN
        1 ctcgataaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg ttgcatgtac
       61 gaattcatta aagaggagaa aggtaccatg gtgagcaagg gcgaggagct gttcaccggg
      121 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      181 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      241 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      301 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      361 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      481 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      541 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      601 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      661 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
      721 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      781 ctcggcatgg acgagctgta caagtaatct agaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      901 caaatccgcc gccctagacc taggggatat attccgcttc ctcgctcact gactcgctac
      961 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     1021 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     1081 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     1141 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     1201 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     1261 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     1321 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1381 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     1441 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     1501 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     1561 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     1621 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
     1681 aatgtagcac ctgaagtcag ccccatacga tataagttgt tactagtgct tggattctca
     1741 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1801 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1861 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1921 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1981 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2041 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     2101 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2161 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     2221 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2281 tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2341 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2401 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2461 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     2521 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2581 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc
     2641 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     2701 cgtcttcac
//
