LOCUS       pQE-80L_2xHis-LRRK2_Arm 5375 bp ds-DNA     circular     04-MAY-2022
DEFINITION  .
KEYWORDS    "creator:Qiagen" "marker:AmpR, CmR" "organism:Escherichia coli"    
FEATURES             Location/Qualifiers
     promoter        10..24
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        25..30
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    30..46
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        31..47
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        48..54
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    62..78
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     RBS             101..106
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             115..117
                     /label="ATG"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             115..144
                     /label="Translation 115-144"
     CDS             145..162
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             145..162
                     /label="Translation 145-162"
     CDS             169..171
                     /label="Translation 169-171"
     misc_feature    172..780
                     /label="ARM domain"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             172..810
                     /label="Translation 172-810"
     terminator      832..926
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             970..1629
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      1694..1780
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1873..2955)
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(2956..3033)
                     /label="lacIq promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(3551..4139)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4310..5101)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5102..5170)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(5171..5275)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGCAT
      121 CACCATCACC ATCACAGAGG ATCGCATCAC CATCACCATC ACGGATCCAT GCTGGAAGCC
      181 TGTTACAAAG CATTAACGTG GCATAGAAAG AACAAGCACG TGCAGGAGGC CGCATGCTGG
      241 GCACTAAATA ATCTCCTTAT GTACCAAAAC AGTTTACATG AGAAGATTGG AGATGAAGAT
      301 GGCCATTTCC CAGCTCATAG GGAAGTGATG CTCTCCATGC TGATGCATTC TTCATCAAAG
      361 GAAGTTTTCC AGGCATCTGC GAATGCATTG TCAACTCTCT TAGAACAAAA TGTTAATTTC
      421 AGAAAAATAC TGTTATCAAA AGGAATACAC CTGAATGTTT TGGAGTTAAT GCAGAAGCAT
      481 ATACATTCTC CTGAAGTGGC TGAAAGTGGC TGTAAAATGC TAAATCATCT TTTTGAAGGA
      541 AGCAACACTT CCCTGGATAT AATGGCAGCA GTGGTCCCCA AAATACTAAC AGTTATGAAA
      601 CGTCATGAGA CATCATTACC AGTGCAGCTG GAGGCGCTTC GAGCTATTTT ACATTTTATA
      661 GTGCCTGGCA TGCCAGAAGA ATCCAGGGAG GATACAGAAT TTCATCATAA GCTAAATATG
      721 GTTAAAAAAC AGTGTTTCAA GAATGATATT CACAAACTGG TCCTAGCAGC TTTGAACAGG
      781 TAGTCTAGAA TTCCCTGCAG GTAATTAAAT AAGCTTAATT AGCTGAGCTT GGACTCCTGT
      841 TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT GTTCAGAACG CTCGGTTGCC
      901 GCCGGGCGTT TTTTATTGGT GAGAATCCAA GCTAGCTTGG CGAGATTTTC AGGAGCTAAG
      961 GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT
     1021 CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT
     1081 CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG
     1141 GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTTCG TATGGCAATG
     1201 AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG
     1261 CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA
     1321 CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG
     1381 TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT
     1441 TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT
     1501 ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTTTGTGAT
     1561 GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC
     1621 GGGGCGTAAT TTTTTTAAGG CAGTTATTGG TGCCCTTAAA CGCCTGGGGT AATGACTCTC
     1681 TAGCTTGAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT
     1741 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCCTCTA GATTACGTGC
     1801 AGTCGATGAT AAGCTGTCAA ACATGAGAAT TGTGCCTAAT GAGTGAGCTA ACTTACATTA
     1861 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
     1921 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCCAGGG TGGTTTTTCT
     1981 TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC GCCTGGCCCT GAGAGAGTTG
     2041 CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA TCCTGTTTGA TGGTGGTTAA
     2101 CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT CCCACTACCG AGATATCCGC
     2161 ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG CCCAGCGCCA TCTGATCGTT
     2221 GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC ATTTGCATGG TTTGTTGAAA
     2281 ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC GGCTGAATTT GATTGCGAGT
     2341 GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG ACAGAACTTA ATGGGCCCGC
     2401 TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC TCCACGCCCA GTCGCGTACC
     2461 GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG TCAGAGACAT CAAGAAATAA
     2521 CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA TCCTGGTCAT CCAGCGGATA
     2581 GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG TGCACCGCCG CTTTACAGGC
     2641 TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG GCACCCAGTT GATCGGCGCG
     2701 AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG GCCAGACTGG AGGTGGCAAC
     2761 GCCAATCAGC AACGACTGTT TGCCCGCCAG TTGTTGTGCC ACGCGGTTGG GAATGTAATT
     2821 CAGCTCCGCC ATCGCCGCTT CCACTTTTTC CCGCGTTTTC GCAGAAACGT GGCTGGCCTG
     2881 GTTCACCACG CGGGAAACGG TCTGATAAGA GACACCGGCA TACTCTGCGA CATCGTATAA
     2941 CGTTACTGGT TTCACATTCA CCACCCTGAA TTGACTCTCT TCCGGGCGCT ATCATGCCAT
     3001 ACCGCGAAAG GTTTTGCACC ATTCGATGGT GTCGGAATTT CGGGCAGCGT TGGGTCCTGG
     3061 CCACGGGTGC GCATGATCTA GAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC
     3121 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA
     3181 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG
     3241 TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC
     3301 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
     3361 TCAGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
     3421 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
     3481 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
     3541 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     3601 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
     3661 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
     3721 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
     3781 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
     3841 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
     3901 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
     3961 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA
     4021 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     4081 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
     4141 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
     4201 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     4261 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
     4321 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     4381 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     4441 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
     4501 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
     4561 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
     4621 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
     4681 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
     4741 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
     4801 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
     4861 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
     4921 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
     4981 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
     5041 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
     5101 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     5161 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
     5221 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
     5281 TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA
     5341 AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCAC
//