LOCUS       Exported                6344 bp DNA     circular SYN 04-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 4, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..212
                     /label=ITR
                     /note="ITR"
     promoter        237..1171
                     /note="CAG promoter"
     misc_feature    1180..1213
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    1276..1309
                     /label=loxP
                     /note="loxP"
     CDS             complement(1320..2636)
                     /label=jGCaMP8s
     CDS             complement(2589..2630)
                     /codon_start=1
                     /label=NES
                     /label=jGCaMP8m (var 686)
                     /translation="NLNELALKLAGLDI"
     misc_feature    complement(2653..2686)
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    complement(2757..2790)
                     /label=loxP
                     /note="loxP"
     misc_feature    2832..3420
                     /label=WPRE
                     /note="WPRE"
     primer_bind     complement(2856..2872)
                     /label=r.WPRE.41rev.02.722
     misc_feature    3448..3680
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3708..3874)
                     /label=ITR
     misc_feature    complement(4112..4700)
                     /label=ori
                     /label=nonstandard type: Origin
     gene            complement(4874..5731)
                     /label=Amp
                     /note="Amp"
     misc_feature    complement(5862..6317)
                     /label=f1-ori
                     /label=nonstandard type: Origin
ORIGIN
        1 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc
       61 tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag
      121 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt
      181 taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag agtaccattg
      241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      481 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      541 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      601 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      661 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      721 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgacgctg
      781 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      841 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      901 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      961 ggagggccct ttgtgcgggg ggagcggctc ggggctgtcc gcggggggac ggctgccttc
     1021 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1081 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     1141 ttgtgctgtc tcatcatttt ggcaaagaat tggatcccca taacttcgta taaagtatcc
     1201 tatacgaagt tatatcaaaa taggaagacc aatgcttcac catcgacccg aattgccaag
     1261 catcaccatc gacccataac ttcgtataat gtatgctata cgaagttata ctagagcttt
     1321 cacttcgctg tcatcatttg tacaaactct tcgtagttta cctgaccatc cccatcgatg
     1381 tctgcttccc tgatcatttc atcaacctct tcatctgtta acttctctcc aaggtttgtc
     1441 atcacgtggc gaagctctgc tgcactgatg tagccattgc catccttatc aaacacaccg
     1501 aacgcttctc taatttcttc ttccgtgtcc ctgtatttca tttttcttgc catcattgtc
     1561 aggaactcag ggaagtcgat tgtgccgtca ccgtcggcat ctacttcatt gatcatgtcc
     1621 tgcagctctg cttctgtggg gttgtgtccg agagaccgca tcaccgtccc cagctccttg
     1681 gttgttattg tcccatcccc gtccttgtca aatagggaga aagcctcttt gtattctgcg
     1741 atctgctctt cagtcagttg gtccggcagg ttgtactcca gcttgtgccc caggatgttg
     1801 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     1861 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     1921 tggatgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     1981 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     2041 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     2101 tcaccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     2161 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatgctccc tccggtaccg
     2221 cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     2281 accatgtgat cgcgcttctc gttggggtct ttcgaaagtt tggactccac gctcaggtag
     2341 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtag
     2401 gcgagctgca cgccgccgtc ctcgatgttg tggcggatgt ggaagttcgc cttgatgccg
     2461 ttcttctgct tgtcggcctt gatatagacg ttgatcttca ggcccatcag catggcgatg
     2521 atcttcacgg cggtggccac ctccttgaag gtcttcttgc gacgcgtgtg atgatggtga
     2581 tgatgcatga tgtccaaacc ggccaacttc aatgccagtt cgttcaggtt acccatggtg
     2641 gcggatccta gtataacttc gtataggata ctttatacga agttatcatt gggattcttc
     2701 ctattttgat ccaagcatca ccatcgaccc tctagtccag atctcaccat cgacccataa
     2761 cttcgtatag catacattat acgaagttat gtccctcgaa gaggttcgaa ttcgatatca
     2821 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2881 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2941 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3001 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3061 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3121 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3181 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3241 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3301 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3361 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3421 atcgataccg tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt
     3481 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3541 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3601 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     3661 tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag
     3721 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     3781 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     3841 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgca ttaatgaatc ggccaacgcg
     3901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     5881 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     5941 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6001 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6061 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6121 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6181 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6241 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6301 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aact
//
