LOCUS       Exported                6221 bp DNA     circular SYN 19-MAY-2022
DEFINITION  An AAV genome that ubiquitously expresses the fluorescent protein 
            mRuby2 from the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-NLS-QPAS-VP16
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL, 
            Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
  TITLE     Engineered AAVs for efficient noninvasive gene delivery to the 
            central and peripheral nervous systems.
  JOURNAL   Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 
            2017 Jun 26.
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4593"; 
            journalName: "Nat Neurosci"; date: "2017-08"; volume: "20"; issue: 
            "8"; pages: "1172-1179"
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
            ORIGDB|GenBank
            LSOWNER|
            VNTNAME|Exported|
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FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /note="/vntifkey=98"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /label=AAV2\ITR\(alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
                     /note="/vntifkey=34"
                     /note="color: #000080"
     enhancer        210..513
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="/vntifkey=9"
                     /note="color: #000080; direction: RIGHT"
     promoter        515..792
                     /label=chicken beta-actin promoter
                     /label=chicken\beta-actin\promoter
                     /note="/vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     primer_bind     1122..1144
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     1206..1225
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    1268..2323
                     /label=NLS-QPAS-VP16
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2348..2936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     primer_bind     2401..2421
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     CDS             2819..2830
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /label=Factor\Xa\site
                     /note="/vntifkey=4"
                     /note="color: #3366cc"
                     /translation="SALN"
     polyA_signal    2968..3444
                     /label=hGH poly(A) signal
                     /label=hGH\poly(A)\signal
                     /note="human growth hormone polyadenylation signal"
                     /note="/vntifkey=25"
                     /note="color: #008000"
     primer_bind     3167..3186
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     repeat_region   3484..3624
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="/vntifkey=34"
                     /note="color: #000080"
     repeat_region   3484..3613
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="/vntifkey=34"
                     /note="color: #000080"
     rep_origin      3699..4154
                     /direction=RIGHT
                     /label=f1 ori
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="/vntifkey=33"
                     /note="color: #ff9966"
     primer_bind     3786..3805
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     3996..4017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     4171..4190
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     4290..4312
                     /label=pGEX 3'
                     /label=pGEX\3'
                     /note="pGEX vectors, reverse primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     4350..4368
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     promoter        4436..4540
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="/vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     CDS             4541..5401
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4759..4778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     rep_origin      5572..6160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/vntifkey=33"
                     /note="color: #ff9966"
     primer_bind     6061..6080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="/vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgcggc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagcgccggc aggaaggaaa tgggcgggga
     1021 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1081 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1141 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1201 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggcccaa
     1261 cggtaccatg tcgcggaggc ggcacagcta cgagaacgat ggtggacaac ctcacaaaag
     1321 gaggaagacg tctccaccgg gcaagaacat gcaggcggtc accgagctgc attcccggct
     1381 gatcgctgcg cagcaggcga tggagcgcga ctattggcgg ttgcgtgaat tggagactcg
     1441 ctaccgcctg gtgttcgacg ctgccgccga tgcggtgatg atcgtctccg ccggcgacat
     1501 gcgcatcgtc gaagccaacc gggcggcggt gaatgcgatc agccgcgtcg agcgcggcaa
     1561 tgacgacctt gcggggcgtg atttcctcgc cgaagtggcg gctgccgatc gcgatgcggt
     1621 gcgcgacatg ctggcccagg tgcgtcagcg cggcaccgca ctcagcgtcc tcgttcatct
     1681 cggccgttac gaccgcgcct ggatgctgcg cggttcgctg atgtcgtccg agcgtcgtca
     1741 ggttttcctg ctgcacttca ccccggtgac cacgactccc gcgatcgacg acgacgacaa
     1801 aggtgtcgtt gcttctgctg ccgacggtgc agagggcgcc tcagacgacg cagaggataa
     1861 gcttagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     1921 tggtccggac gccaccatgg cgtacagccg cgcgcgtacg aaaaacaatt acgggtctac
     1981 catcgagggc ctgctcgatc tcccggacga cgacgccccc gaagaggcgg ggctggcggc
     2041 tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac
     2101 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
     2161 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     2221 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     2281 gcagatgttt accgatgccc ttggaattga cgagtacggt ggggaattcg atatcaagct
     2341 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2401 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2461 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2521 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2581 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2641 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2701 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2761 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2821 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2881 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2941 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3001 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3061 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3121 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3181 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3241 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3301 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3361 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3421 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     3481 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3541 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3601 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3661 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3721 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     3781 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3841 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3901 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3961 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4021 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     4081 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4141 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4201 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4261 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4321 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4381 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5701 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6181 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
