LOCUS       Exported                6797 bp DNA     circular SYN 19-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-NES-iGECInano
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     enhancer        210..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        515..792
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      1268..1277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    1274..1339
                     /label=NES
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     CDS             1340..2896
                     /codon_start=1
                     /label=iGECInano
                     /note="color: #993366"
                     /translation="NLDKMLNTTVTEVRKFLQADRVCVFKFEEDYSGTVSHEAVDDRWI
                     SILKTQVQDRYFMETRGEEYVHGRYQAIADIYTANLVECYRDLLIEFQVRAILAVPILQ
                     GKKLWGLLVAHQLAGPREWQTWEIDFLKQQAVVMGIAIQQPPSEEELSECFRIFDFDGN
                     GFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMVENVQPTCDDEP
                     IHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTI
                     ALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNS
                     AIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGSVIAEDRCAEVESKLGL
                     HYPASFIPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNH
                     LEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIGVM
                     EE"
     misc_feature    1340..1771
                     /label=miRFP670nano3
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1778..1984
                     /label=TnC
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1988..2896
                     /label=miRFP720-NTrun
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2924..3512
                     /label=WPRE
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     CDS             complement(3395..3406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3394"
                     /translation="IEGR"
     polyA_signal    3544..4020
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   4060..4200
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      4275..4730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5012..5116
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5117..5977
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5117 .. 5185 / #ccffcc / signal sequence
                        2: 5186 .. 5977 / #ccffcc
                     Cleavage site after base 5185"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6148..6736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgcggc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagcgccggc aggaaggaaa tgggcgggga
     1021 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1081 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1141 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1201 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggcccaa
     1261 cggtaccgcc accatgggca ttaacgagct tgctcttaag ttggctggac ttgatattaa
     1321 caagactgga ggaggttcta atctcgataa aatgctcaat acaacagtga ccgaggtgcg
     1381 caagttcctg caagcggaca gagtgtgcgt gttcaagttc gaggaagatt actccggcac
     1441 cgtctcgcac gaagccgtgg acgacagatg gattagcatc ctgaagaccc aggtgcagga
     1501 cagatacttc atggaaacca gaggcgagga atacgtccac ggcagatacc aggccatcgc
     1561 cgacatctac acagccaatc tggtcgagtg ctacagagac ctgctgatcg agtttcaggt
     1621 gcgggccatt ctggctgtcc ccatcctgca aggcaagaag ctgtggggcc tgctggtggc
     1681 ccaccaactg gccggccctc gggagtggca gacctgggaa atcgacttcc tgaaacagca
     1741 agctgtcgtc atgggaattg ctattcaaca gccgccaagc gaagaggaac tgagcgagtg
     1801 cttcagaatc ttcgacttcg acggcaacgg cttcatcgac agagaggagt ttggcgacat
     1861 catcagactg accggcgagc agctgaccga cgaggacgtg gacgagatct tcggcgactc
     1921 cgacaccgac aagaacggca gaatcgattt cgacgagttc ctgaagatgg tggaaaacgt
     1981 gcagccgacc tgcgacgatg agccgatcca tatccccggt gccatccaac cgcatggact
     2041 gctgctcgcc ctcgccgccg acatgacgat cgttgccggc agcgacaacc ttcccgaact
     2101 caccggactg gcgatcggcg ccctgatcgg ccgctctgcg gccgatgtct tcgactcgga
     2161 gacgcacaac cgtctgacga tcgccttggc cgagcccggg gcggccgtcg gagcaccgat
     2221 cactgtcggc ttcacgatgc gaaaggacgc aggcttcatc ggctcctggc atcgccatga
     2281 tcagctcatc ttcctcgagc tcgagcctcc ccagcgggac gtcgccgagc cgcaggcgtt
     2341 cttccgccgc accaacagcg ccatccgccg cctgcaggcc gccgaaacct tggaaagcgc
     2401 ctgcgccgcc gcggcgcaag aggtgcggaa gattaccggc ttcgatcggg tgatgatcta
     2461 tcgcttcgcc tccgacttca gcgggtccgt gatcgcagag gatcggtgcg ccgaggtcga
     2521 gtcaaaacta ggcctgcact atcctgcctc attcatcccg gcgcaggccc gtcggctcta
     2581 taccatcaac ccggtacgga tcattcccga tatcaattat cggccggtgc cggtcacccc
     2641 agacctcaat ccggtcaccg ggcggccgat tgatcttagc ttcgccatcc tgcgcagcgt
     2701 ctcgcccaac catctggagt tcatgcgcaa cataggcatg cacggcacga tgtcgatctc
     2761 gattttgcgc ggcgagcgac tgtggggatt gatcgtttgc catcaccgaa cgccgtacta
     2821 cgtcgatctc gatggccgcc aagcctgcaa gagggtcgcc gagaggctgg ccactcagat
     2881 cggcgtgatg gaagagtaag aattcgatat caagcttatc gataatcaac ctctggatta
     2941 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3001 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3061 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3121 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3181 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3241 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3301 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     3361 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3421 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3481 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     3541 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     3601 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     3661 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     3721 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     3781 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     3841 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     3901 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     3961 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4021 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     4081 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     4141 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     4201 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     4261 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4321 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     4381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     4501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     4621 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     4681 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     4741 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4801 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4861 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4921 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4981 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5041 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5101 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5161 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5221 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5281 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5341 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5401 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5461 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5521 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5581 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5641 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5701 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5761 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5821 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5881 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5941 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6781 gccttttgct cacatgt
//
