LOCUS       Exported                5576 bp DNA     circular SYN 19-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pBAD-iGECInano
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..5576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        87..965
                     /label=AraC
                     /note="color: #ffffff"
     CDS             complement(87..965)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        992..1276
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1319..1336
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             1358..2917
                     /codon_start=1
                     /label=iGECInano
                     /note="color: #993366"
                     /translation="ANLDKMLNTTVTEVRKFLQADRVCVFKFEEDYSGTVSHEAVDDRW
                     ISILKTQVQDRYFMETRGEEYVHGRYQAIADIYTANLVECYRDLLIEFQVRAILAVPIL
                     QGKKLWGLLVAHQLAGPREWQTWEIDFLKQQAVVMGIAIQQPPSEEELSECFRIFDFDG
                     NGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMVENVQPTCDDE
                     PIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLT
                     IALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTN
                     SAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGSVIAEDRCAEVESKLG
                     LHYPASFIPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPN
                     HLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIGV
                     MEE"
     misc_feature    1358..1792
                     /label=miRFP670nano3
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1799..2005
                     /label=TnC
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2009..2917
                     /label=miRFP720-NTrun
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     terminator      3138..3224
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      3316..3343
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3362..3453
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    3454..4314
                     /label=bla
                     /note="color: #a6acb3"
     CDS             3454..4314
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3454 .. 3522 / #ccffcc / signal sequence
                        2: 3523 .. 4314 / #ccffcc
                     Cleavage site after base 3522"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4485..5073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    5259..5399
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
ORIGIN
        1 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
       61 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
      121 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
      181 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
      241 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
      301 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
      361 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
      421 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
      481 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
      541 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
      601 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
      661 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
      721 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
      781 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
      841 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
      901 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
      961 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
     1021 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt
     1081 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     1141 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
     1201 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
     1261 tctactgttt ctccataccc gttttttggg ctaacaggag gaattaacca tggggagcca
     1321 ccatcaccat caccatggca gatctgcagc tggtaccgcc aatctcgata aaatgctcaa
     1381 tacaacagtg accgaggtgc gcaagttcct gcaagcggac agagtgtgcg tgttcaagtt
     1441 cgaggaagat tactccggca ccgtctcgca cgaagccgtg gacgacagat ggattagcat
     1501 cctgaagacc caggtgcagg acagatactt catggaaacc agaggcgagg aatacgtcca
     1561 cggcagatac caggccatcg ccgacatcta cacagccaat ctggtcgagt gctacagaga
     1621 cctgctgatc gagtttcagg tgcgggccat tctggctgtc cccatcctgc aaggcaagaa
     1681 gctgtggggc ctgctggtgg cccaccaact ggccggccct cgggagtggc agacctggga
     1741 aatcgacttc ctgaaacagc aagctgtcgt catgggaatt gctattcaac agccgccaag
     1801 cgaagaggaa ctgagcgagt gcttcagaat cttcgacttc gacggcaacg gcttcatcga
     1861 cagagaggag tttggcgaca tcatcagact gaccggcgag cagctgaccg acgaggacgt
     1921 ggacgagatc ttcggcgact ccgacaccga caagaacggc agaatcgatt tcgacgagtt
     1981 cctgaagatg gtggaaaacg tgcagccgac ctgcgacgat gagccgatcc atatccccgg
     2041 tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc gacatgacga tcgttgccgg
     2101 cagcgacaac cttcccgaac tcaccggact ggcgatcggc gccctgatcg gccgctctgc
     2161 ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg atcgccttgg ccgagcccgg
     2221 ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg cgaaaggacg caggcttcat
     2281 cggctcctgg catcgccatg atcagctcat cttcctcgag ctcgagcctc cccagcggga
     2341 cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc gccatccgcc gcctgcaggc
     2401 cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa gaggtgcgga agattaccgg
     2461 cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc agcgggtccg tgatcgcaga
     2521 ggatcggtgc gccgaggtcg agtcaaaact aggcctgcac tatcctgcct cattcatccc
     2581 ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg atcattcccg atatcaatta
     2641 tcggccggtg ccggtcaccc cagacctcaa tccggtcacc gggcggccga ttgatcttag
     2701 cttcgccatc ctgcgcagcg tctcgcccaa ccatctggag ttcatgcgca acataggcat
     2761 gcacggcacg atgtcgatct cgattttgcg cggcgagcga ctgtggggat tgatcgtttg
     2821 ccatcaccga acgccgtact acgtcgatct cgatggccgc caagcctgca agagggtcgc
     2881 cgagaggctg gccactcaga tcggcgtgat ggaagagtag ctgaattcga agcttggctg
     2941 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     3001 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     3061 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     3121 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3181 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     3241 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     3301 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     3361 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3421 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3481 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3541 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3601 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3661 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     3721 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3781 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3841 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3901 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3961 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4021 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4081 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4141 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4201 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4261 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4321 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4381 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4621 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     5281 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5341 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5401 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5461 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5521 caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgca
//
