LOCUS       Exported                5500 bp DNA     circular SYN 19-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piGECI-N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..5500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      595..604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             601..2163
                     /codon_start=1
                     /label=iGECInano
                     /note="color: #993366"
                     /translation="MANLDKMLNTTVTEVRKFLQADRVCVFKFEEDYSGTVSHEAVDDR
                     WISILKTQVQDRYFMETRGEEYVHGRYQAIADIYTANLVECYRDLLIEFQVRAILAVPI
                     LQGKKLWGLLVAHQLAGPREWQTWEIDFLKQQAVVMGIAIQQPPSEEELSECFRIFDFD
                     GNGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMVENVQPTCDD
                     EPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRL
                     TIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRT
                     NSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGSVIAEDRCAEVESKL
                     GLHYPASFIPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSP
                     NHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIG
                     VMEE"
     misc_feature    601..1038
                     /label=miRFP670nano3
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1045..1251
                     /label=TnC
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1255..2163
                     /label=miRFP720-NTrun
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     polyA_signal    2288..2409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(2416..2871)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2898..3002
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3004..3361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3212..3347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3396..4190
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4422..4469
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     rep_origin      4798..5386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggccaatc tcgataaaat gctcaataca acagtgaccg aggtgcgcaa gttcctgcaa
      661 gcggacagag tgtgcgtgtt caagttcgag gaagattact ccggcaccgt ctcgcacgaa
      721 gccgtggacg acagatggat tagcatcctg aagacccagg tgcaggacag atacttcatg
      781 gaaaccagag gcgaggaata cgtccacggc agataccagg ccatcgccga catctacaca
      841 gccaatctgg tcgagtgcta cagagacctg ctgatcgagt ttcaggtgcg ggccattctg
      901 gctgtcccca tcctgcaagg caagaagctg tggggcctgc tggtggccca ccaactggcc
      961 ggccctcggg agtggcagac ctgggaaatc gacttcctga aacagcaagc tgtcgtcatg
     1021 ggaattgcta ttcaacagcc gccaagcgaa gaggaactga gcgagtgctt cagaatcttc
     1081 gacttcgacg gcaacggctt catcgacaga gaggagtttg gcgacatcat cagactgacc
     1141 ggcgagcagc tgaccgacga ggacgtggac gagatcttcg gcgactccga caccgacaag
     1201 aacggcagaa tcgatttcga cgagttcctg aagatggtgg aaaacgtgca gccgacctgc
     1261 gacgatgagc cgatccatat ccccggtgcc atccaaccgc atggactgct gctcgccctc
     1321 gccgccgaca tgacgatcgt tgccggcagc gacaaccttc ccgaactcac cggactggcg
     1381 atcggcgccc tgatcggccg ctctgcggcc gatgtcttcg actcggagac gcacaaccgt
     1441 ctgacgatcg ccttggccga gcccggggcg gccgtcggag caccgatcac tgtcggcttc
     1501 acgatgcgaa aggacgcagg cttcatcggc tcctggcatc gccatgatca gctcatcttc
     1561 ctcgagctcg agcctcccca gcgggacgtc gccgagccgc aggcgttctt ccgccgcacc
     1621 aacagcgcca tccgccgcct gcaggccgcc gaaaccttgg aaagcgcctg cgccgccgcg
     1681 gcgcaagagg tgcggaagat taccggcttc gatcgggtga tgatctatcg cttcgcctcc
     1741 gacttcagcg ggtccgtgat cgcagaggat cggtgcgccg aggtcgagtc aaaactaggc
     1801 ctgcactatc ctgcctcatt catcccggcg caggcccgtc ggctctatac catcaacccg
     1861 gtacggatca ttcccgatat caattatcgg ccggtgccgg tcaccccaga cctcaatccg
     1921 gtcaccgggc ggccgattga tcttagcttc gccatcctgc gcagcgtctc gcccaaccat
     1981 ctggagttca tgcgcaacat aggcatgcac ggcacgatgt cgatctcgat tttgcgcggc
     2041 gagcgactgt ggggattgat cgtttgccat caccgaacgc cgtactacgt cgatctcgat
     2101 ggccgccaag cctgcaagag ggtcgccgag aggctggcca ctcagatcgg cgtgatggaa
     2161 gagtgagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     2221 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2281 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2401 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2461 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2521 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2581 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2641 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2701 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2761 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2821 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     2881 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2941 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3001 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3181 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3301 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3361 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     3421 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3481 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3541 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     3601 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3661 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3721 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3781 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3841 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3901 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     3961 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4021 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4081 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4141 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4201 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4261 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4321 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     4381 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     4441 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4501 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4561 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4621 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     4681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4921 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5461 atcccctgat tctgtggata accgtattac cgccatgcat
//
