LOCUS       Exported               10721 bp ds-DNA    circular SYN 09-5ÔÂ-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-Puro-KRAB-STAT3-3xFlag
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   xfbai1840
  TITLE     Direct Submission
  JOURNAL   Exported 2022Äê5ÔÂ9ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2144
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5347..5418
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /note="3xFLAG"
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    5424..5997
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6030..6629
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6643..7231
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7114..7125)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             7438..8071
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8199..8215)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8223..8239
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8247..8277)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8292..8313
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8601..9189)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9360..10220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10221..10325)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    10373..10507
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgggg
     2821 acactggtga ccttcaagga tgtgtttgtg gacttcacca gggaggagtg gaagctgctg
     2881 gacactgctc agcagatcct gtacagaaat gtgatgctgg agaactataa gaacctggtt
     2941 tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa gggagaagag
     3001 ccctggctgc tcgagagcgg ccgcactagt atggcccaat ggaatcagct acagcagctt
     3061 gacacacggt acctggagca gctccatcag ctctacagtg acagcttccc aatggagctg
     3121 cggcagtttc tggccccttg gattgagagt caagattggg catatgcggc cagcaaagaa
     3181 tcacatgcca ctttggtgtt tcataatctc ctgggagaga ttgaccagca gtatagccgc
     3241 ttcctgcaag agtcgaatgt tctctatcag cacaatctac gaagaatcaa gcagtttctt
     3301 cagagcaggt atcttgagaa gccaatggag attgcccgga ttgtggcccg gtgcctgtgg
     3361 gaagaatcac gccttctaca gactgcagcc actgcggccc agcaaggggg ccaggccaac
     3421 caccccacag cagccgtggt gacggagaag cagcagatgc tggagcagca ccttcaggat
     3481 gtccggaaga gagtgcagga tctagaacag aaaatgaaag tggtagagaa tctccaggat
     3541 gactttgatt tcaactataa aaccctcaag agtcaaggag acatgcaaga tctgaatgga
     3601 aacaaccagt cagtgaccag gcagaagatg cagcagctgg aacagatgct cactgcgctg
     3661 gaccagatgc ggagaagcat cgtgagtgag ctggcggggc ttttgtcagc gatggagtac
     3721 gtgcagaaaa ctctcacgga cgaggagctg gctgactgga agaggcggca acagattgcc
     3781 tgcattggag gcccgcccaa catctgccta gatcggctag aaaactggat aacgtcatta
     3841 gcagaatctc aacttcagac ccgtcaacaa attaagaaac tggaggagtt gcagcaaaaa
     3901 gtttcctaca aaggggaccc cattgtacag caccggccga tgctggagga gagaatcgtg
     3961 gagctgttta gaaacttaat gaaaagtgcc tttgtggtgg agcggcagcc ctgcatgccc
     4021 atgcatcctg accggcccct cgtcatcaag accggcgtcc agttcactac taaagtcagg
     4081 ttgctggtca aattccctga gttgaattat cagcttaaaa ttaaagtgtg cattgacaaa
     4141 gactctgggg acgttgcagc tctcagagga tcccggaaat ttaacattct gggcacaaac
     4201 acaaaagtga tgaacatgga agaatccaac aacggcagcc tctctgcaga attcaaacac
     4261 ttgaccctga gggagcagag atgtgggaat gggggccgag ccaattgtga tgcttccctg
     4321 attgtgactg aggagctgca cctgatcacc tttgagaccg aggtgtatca ccaaggcctc
     4381 aagattgacc tagagaccca ctccttgcca gttgtggtga tctccaacat ctgtcagatg
     4441 ccaaatgcct gggcgtccat cctgtggtac aacatgctga ccaacaatcc caagaatgta
     4501 aactttttta ccaagccccc aattggaacc tgggatcaag tggccgaggt cctgagctgg
     4561 cagttctcct ccaccaccaa gcgaggactg agcatcgagc agctgactac actggcagag
     4621 aaactcttgg gacctggtgt gaattattca gggtgtcaga tcacatgggc taaattttgc
     4681 aaagaaaaca tggctggcaa gggcttctcc ttctgggtct ggctggacaa tatcattgac
     4741 cttgtgaaaa agtacatcct ggccctttgg aacgaagggt acatcatggg ctttatcagt
     4801 aaggagcggg agcgggccat cttgagcact aagcctccag gcaccttcct gctaagattc
     4861 agtgaaagca gcaaagaagg aggcgtcact ttcacttggg tggagaagga catcagcggt
     4921 aagacccaga tccagtccgt ggaaccatac acaaagcagc agctgaacaa catgtcattt
     4981 gctgaaatca tcatgggcta taagatcatg gatgctacca atatcctggt gtctccactg
     5041 gtctatctct atcctgacat tcccaaggag gaggcattcg gaaagtattg tcggccagag
     5101 agccaggagc atcctgaagc tgacccaggt agcgctgccc catacctgaa gaccaagttt
     5161 atctgtgtga caccaacgac ctgcagcaat accattgacc tgccgatgtc cccccgcact
     5221 ttagattcat tgatgcagtt tggaaataat ggtgaaggtg ctgaaccctc agcaggaggg
     5281 cagtttgagt ccctcacctt tgacatggag ttgacctcgg agtgcgctac ctcccccatg
     5341 ggatccgact acaaggatga cgatgacaag gattacaaag acgacgatga taaggactat
     5401 aaggatgatg acgacaaatg acgcccctct ccctcccccc cccctaacgt tactggccga
     5461 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     5521 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5581 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5641 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5701 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5761 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5821 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5881 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5941 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     6001 tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     6061 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6121 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     6181 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6241 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6301 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6361 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6421 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6481 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6541 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6601 ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga acaatcaacc tctggattac
     6661 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6721 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6781 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6841 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6901 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6961 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7021 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     7081 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7141 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7201 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     7261 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     7321 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     7381 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     7441 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     7501 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7561 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7621 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7681 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7741 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7801 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7861 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7921 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7981 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8041 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8101 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     8161 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8221 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8281 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8341 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8401 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9001 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9241 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9301 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9361 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9421 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9481 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9541 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9601 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9661 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9721 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9781 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9841 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9901 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9961 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10021 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10081 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10141 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10201 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10261 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10321 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
    10381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10501 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10561 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10621 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa
    10681 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
