LOCUS       Exported                6944 bp ds-DNA     circular SYN 22-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          225..231
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          232..259
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          1406..4882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(7..36)
                     /label=oBFC2045
     misc_feature    7..31
                     /label=ConS scar
     misc_feature    7..31
                     /label=ConS scar
     protein_bind    7..10
                     /label=BsmBI
     primer_bind     10..31
                     /label=oBFC1841
     primer_bind     30..59
                     /label=oBFC2036
     misc_feature    32..652
                     /label=gBlock 2 (guide RNA)
     misc_feature    32..39
                     /label=Not on PCR assembled transcript
     misc_feature    32..35
                     /label=BsmBI_OH
     primer_bind     38..66
                     /label=oBFC0910
     primer_bind     40..76
                     /label=oBFC0166
     misc_feature    40..43
                     /label=BsaI Junction Scar
     terminator      44..104
                     /label=L3S2P21 Terminator (Voigt)
                     /note="Strongest synthetic promoter from Voigt's Nature 
                     Methods paper
                     
                     Also used by Alex Nielsen as a dSpyCas9 sgRNA terminator in
                     his CRISPRi logic gates paper
                     "
     terminator      44..104
                     /label=L3S2P21 Terminator (Voigt)
                     /note="Strongest synthetic promoter from Voigt's Nature 
                     Methods paper
                     
                     Also used by Alex Nielsen as a dSpyCas9 sgRNA terminator in
                     his CRISPRi logic gates paper
                     "
     primer_bind     complement(81..108)
                     /label=oBFC0169
     protein_bind    105..108
                     /label=BsaI
     primer_bind     complement(109..137)
                     /label=oBFC1766
     misc_feature    109..133
                     /label=Con1 scar
     misc_feature    109..133
                     /label=Con1 scar
     protein_bind    109..112
                     /label=BsmBI
     primer_bind     complement(111..133)
                     /label=oBFC1305
     misc_feature    134..137
                     /label=BsaI Pt2 OH
     terminator      138..181
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     172..201
                     /label=oBA1606_crRNA_check_BFC_F
     primer_bind     188..209
                     /label=oBFC0271
     promoter        190..224
                     /label=J23119
                     /label=J23119 (SpeI) promoter
     primer_bind     complement(190..219)
                     /label=oBFC0971
     primer_bind     190..212
                     /label=oBFC0973
     primer_bind     190..212
                     /label=oBA1989_J23119_PCR_F
     primer_bind     191..212
                     /label=oBFC1338
     primer_bind     complement(193..219)
                     /label=oBFC0545
     primer_bind     complement(193..219)
                     /label=oBFC0201
     primer_bind     complement(193..219)
                     /label=oBFC0537
     primer_bind     complement(193..219)
                     /label=oBFC0553
     primer_bind     complement(193..219)
                     /label=oBFC0179
     primer_bind     complement(193..219)
                     /label=oBFC0375
     primer_bind     complement(193..219)
                     /label=oBFC0386
     primer_bind     complement(194..223)
                     /label=oBFC1913
     misc_feature    225..259
                     
     misc_feature    complement(230)
                     /label=Processing
     primer_bind     256..286
                     /label=Guide_F
     misc_feature    256..259
                     /label=BsaI
     primer_bind     complement(260..290)
                     /label=Guide_R
     misc_feature    260..285
                     /label=2xBsaI_stuffer
     misc_feature    complement(261..266)
                     /label=BsaI
     primer_bind     complement(267..285)
                     /label=oBFC1291_fixed
     primer_bind     complement(267..285)
                     /label=oBFC1154
     primer_bind     complement(268..285)
                     /label=oBFC1132
     misc_feature    280..285
                     /label=BsaI
     terminator      286..652
                     /label=TrrnB terminator (BBa_K1893035)
                     /label=TrrnB
     misc_feature    287..290
                     /label=BsaI
     misc_feature    287
                     /label=a>t removes HindIII
     primer_bind     complement(335..360)
                     /label=oBA1607_crRNA_check_BFC_R
     misc_feature    join(373,386)
                     /label=covariant muts to remove BsaI
     misc_feature    join(555,572)
                     /label=covariant muts to remove BsaI
     primer_bind     complement(575..601)
                     /label=Primer 1
     primer_bind     577..601
                     /label=oBFC1711
     primer_bind     578..609
                     /label=oBFC0174
     terminator      586..631
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     586..607
                     /label=oBFC0177
     primer_bind     595..626
                     /label=Primer 3
     primer_bind     complement(595..626)
                     /label=Primer 3
     primer_bind     595..621
                     /label=Primer 4
     primer_bind     595..618
                     /label=Primer 2
     misc_feature    597..600
                     /label=BsmBI OH?
     primer_bind     complement(614..651)
                     /label=oBFC0177
     primer_bind     complement(625..654)
                     /label=oBFC2037
     primer_bind     648..677
                     /label=oBFC2038
     primer_bind     649..677
                     /label=oBFC0336
     misc_feature    649..652
                     /label=BsmBI_OH
     primer_bind     663..686
                     /label=oBFC1697
     CDS             complement(670..1293)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1186..1212
                     /label=KEW.A01
     primer_bind     1237..1259
                     /label=oBFC0419
     primer_bind     complement(1256..1293)
                     /label=oBFC0604
     primer_bind     1297..1340
                     /label=oBFC1701
     promoter        1312..1367
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     primer_bind     complement(1333..1367)
                     /label=oBFC0337
     primer_bind     complement(1333..1367)
                     /label=oBFC0338
     primer_bind     complement(1345..1369)
                     /label=oBFC1704
     primer_bind     1347..1365
                     /label=oBFC1899
     protein_bind    1348..1366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(1349..1366)
                     /label=oBFC1708
     primer_bind     complement(1363..1408)
                     /label=oBA1595_Cas13_BB_CommonGib_R
     primer_bind     complement(1363..1384)
                     /label=oBFC1698
     primer_bind     1375..1434
                     /label=oBA1598_Cas13a_p1_Common_F
     primer_bind     complement(1384..1398)
                     /label=oBFC0283
     RBS             1385..1396
                     /label=RBS (strong)
     primer_bind     1392..1431
                     /label=Primer 66
     primer_bind     1399..1424
                     /label=EJC078
     primer_bind     1404..1429
                     /label=EJC003
     primer_bind     1405..1429
                     /label=Primer 1a
     primer_bind     1405..1426
                     /label=Primer 26
     CDS             1406..4885
                     /codon_start=1
                     /label=Lbu_C2c2_WT
                     /label=WP_015770004.1
                     /translation="MKVTKVGGISHKKYTSEGRLVKSESEENRTDERLSALLNMRLDMY
                     IKNPSSTETKENQKRIGKLKKFFSNKMVYLKDNTLSLKNGKKENIDREYSETDILESDV
                     RDKKNFAVLKKIYLNENVNSEELEVFRNDIKKKLNKINSLKYSFEKNKANYQKINENNI
                     EKVEGKSKRNIIYDYYRESAKRDAYVSNVKEAFDKLYKEEDIAKLVLEIENLTKLEKYK
                     IREFYHEIIGRKNDKENFAKIIYEEIQNVNNMKELIEKVPDMSELKKSQVFYKYYLDKE
                     ELNDKNIKYAFCHFVEIEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLLNK
                     LDTYVRNCGKYNYYLQDGEIATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETENE
                     NDITGRMRGKTVKNNKGEEKYVSGEVDKIYNENKKNEVKENLKMFYSYDFNMDNKNEIE
                     DFFANIDEAISSIRHGIVHFNLELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLKIF
                     RQLNSANVFRYLEKYKILNYLKRTRFEFVNKNIPFVPSFTKLYSRIDDLKNSLGIYWKT
                     PKTNDDNKTKEIIDAQIYLLKNIYYGEFLNYFMSNNGNFFEISKEIIELNKNDKRNLKT
                     GFYKLQKFEDIQEKIPKEYLANIQSLYMINAGNQDEEEKDTYIDFIQKIFLKGFMTYLA
                     NNGRLSLIYIGSDEETNTSLAEKKQEFDKFLKKYEQNNNIKIPYEINEFLREIKLGNIL
                     KYTERLNMFYLILKLLNHKELTNLKGSLEKYQSANKEEAFSDQLELINLLNLDNNRVTE
                     DFELEADEIGKFLDFNGNKVKDNKELKKFDTNKIYFDGENIIKHRAFYNIKKYGMLNLL
                     EKIADKAGYKISIEELKKYSNKKNEIEKNHKMQENLHRKYARPRKDEKFTDEDYESYKQ
                     AIENIEEYTHLKNKVEFNELNLLQGLLLRILHRLVGYTSIWERDLRFRLKGEFPENQYI
                     EEIFNFENKKNVKYKGGQIVEKYIKFYKELHQNDEVKINKYSSANIKVLKQEKKDLYIR
                     NYIAHFNYIPHAEISLLEVLENLRKLLSYDRKLKNAVMKSVVDILKEYGFVATFKIGAD
                     KKIGIQTLESEKIVHLKNLKKKKLMTDRNSEELCKLVKIMFEYKMEEKKSEN"
     misc_feature    1406..2612
                     /label=WP_015770004.1_gB1
     primer_bind     1406..1429
                     /label=Primer 10
     primer_bind     complement(1458..1480)
                     /label=Primer 63
     primer_bind     complement(1459..1482)
                     /label=Primer 62
     primer_bind     complement(1459..1482)
                     /label=Primer 46
     primer_bind     1463..1506
                     /label=Primer 61
     primer_bind     1484..1506
                     /label=Primer 64
     primer_bind     1484..1506
                     /label=Primer 45
     primer_bind     1876..1903
                     /label=EJC039
     primer_bind     2019..2078
                     /label=Primer 32
     primer_bind     complement(2078..2107)
                     /label=Primer 29
     primer_bind     complement(2078..2107)
                     /label=EJC302
     primer_bind     complement(2078..2107)
                     /label=EJC300
     primer_bind     2166..2225
                     /label=Primer 31
     primer_bind     complement(2171..2194)
                     /label=Primer 69
     primer_bind     2174..2231
                     /label=Primer 68
     primer_bind     2193..2225
                     /label=Primer 65
     primer_bind     complement(2255..2281)
                     /label=EJC341
     primer_bind     2284..2312
                     /label=EJC348
     primer_bind     2284..2312
                     /label=EJC340
     primer_bind     2284..2312
                     /label=EJC339
     primer_bind     complement(2417..2446)
                     /label=EJC344
     primer_bind     2449..2486
                     /label=EJC349
     primer_bind     2449..2486
                     /label=Primer 18
     primer_bind     2449..2486
                     /label=EJC343
     primer_bind     2449..2486
                     /label=EJC342
     primer_bind     complement(2465..2489)
                     /label=Primer 23
     primer_bind     2468..2486
                     /label=EJC040
     primer_bind     complement(2561..2587)
                     /label=Primer 13
     misc_feature    2588..2590
                     /label=Feature 19
     primer_bind     2589..2617
                     /label=Primer 15
     primer_bind     complement(2596..2623)
                     /label=EJC394
     primer_bind     2616..2675
                     /label=Primer 33
     primer_bind     2626..2657
                     /label=EJC393
     primer_bind     complement(2669..2710)
                     /label=Primer 16
     primer_bind     2696..2727
                     /label=Primer 28
     primer_bind     2696..2727
                     /label=Primer 27
     misc_feature    2711..2713
                     /label=Feature 20
     primer_bind     2712..2753
                     /label=Primer 14
     primer_bind     complement(2796..2828)
                     /label=Primer 53
     primer_bind     complement(2821..2848)
                     /label=Primer 20
     misc_feature    2821..2833
                     /label=Active Site 1
     primer_bind     2825..2869
                     /label=Primer 52
     primer_bind     2851..2882
                     /label=Primer 19
     primer_bind     3006..3065
                     /label=Primer 34
     primer_bind     complement(3008..3035)
                     /label=Primer 48
     primer_bind     3037..3067
                     /label=Primer 47
     primer_bind     3082..3101
                     /label=EJC041
     CDS             3248..3295
                     /codon_start=1
                     /label=Feature 23
                     /translation="FFEISKEIIELNKNDK"
     primer_bind     complement(3312..3344)
                     /label=oBFC2039
     primer_bind     3324..3404
                     /label=Primer 39
     primer_bind     3324..3383
                     /label=Primer 38
     primer_bind     complement(3325..3353)
                     /label=Primer 44
     primer_bind     3341..3371
                     /label=oBFC2040
     primer_bind     3345..3425
                     /label=Primer 37
     primer_bind     3345..3404
                     /label=Primer 35
     CDS             3353..3400
                     /codon_start=1
                     /label=Feature 22
                     /translation="IPKEYLANIQSLYMIN"
     primer_bind     3355..3382
                     /label=Primer 43
     primer_bind     complement(3355..3375)
                     /label=Primer 50
     primer_bind     3366..3425
                     /label=Primer 36
     primer_bind     3513..3572
                     /label=Primer 40
     primer_bind     complement(3523..3556)
                     /label=Primer 56
     primer_bind     3537..3596
                     /label=Primer 58
     primer_bind     3537..3596
                     /label=Primer 57
     primer_bind     3537..3592
                     /label=Primer 51
     primer_bind     3538..3597
                     /label=Primer 41
     primer_bind     3558..3617
                     /label=Primer 42
     primer_bind     3580..3639
                     /label=Primer 25
     misc_feature    3609..3614
                     /label=one off BamHI site
     primer_bind     complement(3636..3661)
                     /label=Primer 22
     primer_bind     3664..3697
                     /label=Primer 21
     primer_bind     3707..3729
                     /label=EJC042
     primer_bind     complement(3791..3823)
                     /label=oBA1599_Cas13a_p1_Common_R
     primer_bind     3791..3823
                     /label=oBA1600_Cas13a_p2_Common_F
     primer_bind     complement(3797..3829)
                     /label=Primer 60
     primer_bind     3810..3858
                     /label=Primer 59
     primer_bind     3864..3899
                     /label=Primer 17
     misc_feature    3998..4345
                     /label=Phyre aligned section
     primer_bind     complement(4037..4059)
                     /label=oBFC2041
     primer_bind     4057..4097
                     /label=oBFC2042
     primer_bind     4256..4273
                     /label=EJC043
     primer_bind     complement(4513..4557)
                     /label=Primer 55
     misc_feature    4550..4567
                     /label=Active Site 2
     primer_bind     4553..4595
                     /label=Primer 54
     primer_bind     complement(4804..4825)
                     /label=EJC347
     primer_bind     4828..4867
                     /label=EJC350
     primer_bind     4828..4867
                     /label=EJC346
     primer_bind     4828..4867
                     /label=EJC345
     primer_bind     complement(4845..4882)
                     /label=EJC004
     primer_bind     complement(4846..4889)
                     /label=oBFC2043
     primer_bind     complement(4850..4909)
                     /label=oBA1601_Cas13a_p2_Common_R
     primer_bind     4882..4919
                     /label=oBA1597_Cas13_BB_CommonGib_F
     misc_feature    4883..4885
                     /label=BsmBI_OH
     primer_bind     4884..4908
                     /label=oBFC2044
     primer_bind     4886..4920
                     /label=oBFC1103
     primer_bind     4886..4911
                     /label=oBFC1093
     primer_bind     4886..4908
                     /label=oBFC1105
     primer_bind     4888..4924
                     /label=oBFC0170
     primer_bind     4888..4917
                     /label=oBFC0902
     primer_bind     4888..4917
                     /label=oBFC0908
     primer_bind     4888..4908
                     /label=oBFC0811
     misc_feature    join(4889..6944,1..31)
                     /label=PCR from pBFC0335 or similar
     misc_feature    4889..4892
                     /label=BsaI Junction Scar
     terminator      4893..4949
                     /label=L3S2P55 Terminator (Voigt)
                     /note="A strong synthetic promoter from Voigt's Nature 
                     Methods paper
                     
                     Also used by Alex Nielsen as a dSpyCas9 sgRNA terminator in
                     his CRISPRi logic gates paper
                     "
     terminator      4893..4949
                     /label=L3S2P55 Terminator (Voigt)
                     /note="A strong synthetic promoter from Voigt's Nature 
                     Methods paper
                     
                     Also used by Alex Nielsen as a dSpyCas9 sgRNA terminator in
                     his CRISPRi logic gates paper
                     "
     primer_bind     4893..4924
                     /label=oBFC0370
     primer_bind     4893..4920
                     /label=oBFC0683
     primer_bind     4893..4920
                     /label=oBFC0423
     primer_bind     4893..4920
                     /label=oBFC0427
     primer_bind     4893..4920
                     /label=oBFC1193
     primer_bind     complement(4929..4953)
                     /label=oBFC0173
     primer_bind     complement(4950..4974)
                     /label=oBFC1842
     protein_bind    4950..4953
                     /label=BsaI
     primer_bind     complement(4955..4978)
                     /label=oBFC1633
     misc_feature    4976..4995
                     /label=corrected after full plasmid sequencing
     primer_bind     5028..5075
                     /label=oBFC0058
     primer_bind     complement(5030..5055)
                     /label=oBFC0585
     primer_bind     5053..5080
                     /label=oBFC0631
     primer_bind     5053..5080
                     /label=oBFC0635
     primer_bind     5054..5080
                     /label=oBFC0404
     primer_bind     5054..5075
                     /label=oBFC1833
     misc_feature    complement(5055..5163)
                     /label=CamR Terminator
     misc_feature    complement(5055..5163)
                     /label=CamR Terminator
     primer_bind     5055..5080
                     /label=oBFC1615
     primer_bind     5055..5080
                     /label=oBFC0458
     primer_bind     5055..5074
                     /label=oBFC1058_fixed
     primer_bind     5055..5074
                     /label=oBFC1060_fixed
     primer_bind     5055..5074
                     /label=oBFC1062_fixed
     primer_bind     5055..5074
                     /label=oBFC1447
     primer_bind     complement(5076..5100)
                     /label=oBFC0402
     primer_bind     complement(5115..5144)
                     /label=oBFC0063
     CDS             complement(5164..5823)
                     /label=CamR
     CDS             complement(5164..5823)
                     /label=CamR
     primer_bind     5164..5188
                     /label=oBFC1541
     primer_bind     5164..5188
                     /label=oBFC1613
     primer_bind     complement(5564..5595)
                     /label=oBFC0581
     primer_bind     complement(5569..5593)
                     /label=oBFC1138_fixed
     primer_bind     5588..5619
                     /label=oBFC0580
     primer_bind     5589..5619
                     /label=oBFC1133
     misc_feature    5590..5593
                     /label=BbsI OH
     primer_bind     complement(5794..5835)
                     /label=oBFC1540
     promoter        complement(5824..5928)
                     /label=CamR Promoter
     promoter        complement(5824..5928)
                     /label=CamR Promoter
     primer_bind     5895..5920
                     /label=oBFC0401
     primer_bind     complement(5905..5931)
                     /label=oBFC0059
     primer_bind     complement(5905..5929)
                     /label=oBFC0630
     primer_bind     complement(5905..5928)
                     /label=oBFC1057_fixed
     primer_bind     complement(5905..5928)
                     /label=oBFC1059_fixed
     primer_bind     complement(5905..5928)
                     /label=oBFC1061_fixed
     primer_bind     complement(5905..5928)
                     /label=oBFC1446
     primer_bind     complement(5905..5928)
                     /label=oBFC1832
     primer_bind     complement(5909..5929)
                     /label=oBFC0457
     primer_bind     complement(5909..5929)
                     /label=oBFC0403
     primer_bind     complement(5909..5929)
                     /label=oBFC0634
     primer_bind     5922..5950
                     /label=oBFC0060
     primer_bind     5929..5950
                     /label=oBFC0584
     rep_origin      complement(6196..6741)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     rep_origin      complement(6196..6741)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     misc_feature    6297
                     /label=corrected after full plasmid sequencing
     primer_bind     6811..6837
                     /label=oBFC0066
     primer_bind     complement(6817..6896)
                     /label=oBFC0061
     primer_bind     complement(6817..6841)
                     /label=oBFC1614
     terminator      6842..6931
                     /label=ECK120029600 Terminator (Voigt)
                     /note="rho-independent terminator of spheroplast protein Y 
                     transcript"
     terminator      6842..6931
                     /label=ECK120029600 Terminator (Voigt)
                     /note="rho-independent terminator of spheroplast protein Y 
                     transcript"
     primer_bind     complement(join(6929..6944,1..10))
                     /label=oBFC1293
     primer_bind     complement(join(6929..6944,1..10))
                     /label=oBFC1312
ORIGIN
        1 tctagactga actggccgat aattgcagac ggtttaaaca tccctcggta ccaaattcca
       61 gaaaagaggc ctcccgaaag gggggccttt tttcgttttg gtccgctgcc aaaccagatg
      121 tcaacacagc tacaacgatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      181 ctggaattct tgacagctag ctcagtccta ggtataatac tagtatttag accaccccaa
      241 aaatgaaggg gactaaaaca gagacctcgt ttacctatcg gtctcatgct tgggcccgaa
      301 caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca tcatcatcat
      361 tgagtttaaa cgctctccag cttgggtgtt ttggcggatg agagaagatt ttcagcctga
      421 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
      481 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
      541 gtagtgtggg gtctgcccat gcgagagtag gcaactgcca ggcatcaaat aaaacgaaag
      601 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa ctcgccagat
      661 atcgacgtct taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa
      721 ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg
      781 tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg
      841 atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata
      901 taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt
      961 ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact
     1021 tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc
     1081 ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag
     1141 caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg
     1201 ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct
     1261 gttaatcact ttacttttat ctaatctaga catcattaat tcctaatttt tgttgacact
     1321 ctatcgttga tagagttatt ttaccactcc ctatcagtga tagagaaaag aattcaaaag
     1381 atctaaagag gagaaaggat cttacatgaa agtgacgaag gtaggaggca tttcgcataa
     1441 gaagtacacg tccgaaggcc gcttagtgaa gtcagaatcg gaagaaaatc gcacagacga
     1501 acgtctgtcg gcgttgctta atatgcgcct tgacatgtat atcaagaatc ccagcagcac
     1561 ggaaaccaag gaaaatcaaa aacgcattgg gaaattaaag aaattcttct caaacaaaat
     1621 ggtctatctt aaagacaata ccttgagttt gaagaatggg aaaaaggaga acattgatcg
     1681 tgagtattct gagactgaca tccttgagag cgatgtccgt gacaagaaaa acttcgccgt
     1741 gttgaaaaag atctatctga atgaaaacgt gaactcggag gaattggaag tttttcgtaa
     1801 cgacattaag aagaaactga acaaaatcaa cagcctgaag tactcatttg aaaagaataa
     1861 ggcgaattat caaaagatta atgagaataa catcgagaag gttgaaggta agtcaaagcg
     1921 taacattatt tacgattatt atcgtgagtc agcgaaacgt gacgcttatg taagcaatgt
     1981 gaaagaagcc tttgataagc tttacaagga agaggacatt gcaaaacttg ttcttgaaat
     2041 tgagaacctt acgaagttag agaaatacaa gattcgcgag ttctaccacg aaattattgg
     2101 acgtaagaat gacaaggaaa actttgcaaa aatcatctac gaagaaatcc agaatgttaa
     2161 taacatgaaa gagttgatcg agaaggtacc ggacatgagt gagttgaaaa agagccaagt
     2221 attttacaag tattacttag acaaagaaga gttgaacgac aagaacatca aatacgcgtt
     2281 ttgtcatttc gtggaaatcg aaatgagtca gttgctgaag aactacgtat ataagcgctt
     2341 aagtaatatc tcgaatgaca aaattaagcg tatctttgaa taccagaact tgaaaaaatt
     2401 gatcgaaaat aagctgttaa acaaacttga cacgtacgtc cgtaattgtg gaaagtataa
     2461 ttattatttg caagacggcg aaattgccac ttcagatttc atcgcccgca accgtcagaa
     2521 tgaagcgttt cttcgcaaca tcattggggt gtcatctgtg gcctactttt ctcttcgcaa
     2581 cattcttgaa acggagaacg agaatgatat tactgggcgt atgcgcggca aaacagttaa
     2641 gaacaataaa ggtgaagaga agtacgtgtc cggagaagtt gataagatct ataatgaaaa
     2701 taagaagaac gaggttaagg agaacttaaa aatgttctat tcgtacgatt tcaatatgga
     2761 caacaagaat gaaatcgaag atttcttcgc caacatcgac gaggcgattt cttccatccg
     2821 tcacggtatt gtccacttca acttggaatt agaaggtaag gatatctttg cgttcaagaa
     2881 cattgcgcca tccgaaatct caaagaagat gtttcagaat gagattaacg agaaaaaact
     2941 gaaattgaag atctttcgtc aactgaactc tgccaacgtg ttccgctatc tcgaaaagta
     3001 taaaattctg aattacctta aacgtacacg cttcgagttt gtcaataaaa atatcccatt
     3061 cgtcccgtct ttcaccaaat tatattcgcg cattgatgac ctgaagaata gtcttgggat
     3121 ttactggaaa actccgaaaa caaacgacga caataagact aaggagatta ttgatgccca
     3181 aatctatttg cttaaaaaca tctattacgg ggagttcctg aattatttca tgtcgaacaa
     3241 tggtaatttc tttgagattt ctaaagaaat catcgaattg aacaagaacg ataaacgcaa
     3301 cttaaagact gggttttaca agctgcaaaa gtttgaagac atccaggaga agattccaaa
     3361 ggaatacttg gcgaatatcc agtccctgta catgattaat gccggtaatc aggacgaaga
     3421 agaaaaggac acttatattg atttcattca aaagatcttc ttaaagggat ttatgacgta
     3481 tcttgctaat aacggtcgtt taagtctgat ttacatcggc tcggatgaag aaacaaatac
     3541 gtcattagca gaaaagaagc aagagtttga caagttcttg aagaagtacg agcagaacaa
     3601 taatatcaag atcccctatg agatcaatga attcctgcgt gagatcaaac tgggaaacat
     3661 cctgaagtat actgagcgtt taaacatgtt ctaccttatc ttaaagcttt tgaatcacaa
     3721 ggagctgaca aatctgaagg gtagtcttga aaaatatcag tctgccaata aggaagaagc
     3781 gttctctgac caattggagt taattaacct gcttaacctt gacaacaacc gcgtgacgga
     3841 agacttcgaa ttagaggccg acgagattgg aaaatttctt gatttcaatg gcaacaaagt
     3901 taaggataac aaggaactga aaaagttcga tacaaacaag atctactttg acggcgagaa
     3961 cattatcaaa caccgtgcct tctacaatat taagaaatat ggcatgttaa acttactgga
     4021 gaaaattgcc gacaaggctg gatacaagat ctcgatcgaa gagctgaaga aatactccaa
     4081 taaaaagaat gagatcgaga agaaccataa gatgcaggaa aatctgcacc gcaaatacgc
     4141 tcgtccccgt aaagacgaga agtttacaga tgaggactat gaaagttaca agcaagctat
     4201 tgagaatatt gaggagtaca cccaccttaa gaacaaggta gaattcaatg agctgaattt
     4261 actgcagggc ctgttgctgc gcattttaca tcgtttagtc ggatatacct caatttggga
     4321 acgcgatctg cgcttccgcc ttaaaggtga gttcccagaa aaccaataca tcgaagagat
     4381 cttcaacttt gaaaataaga agaacgtgaa gtacaaaggg ggtcagattg tagagaaata
     4441 cattaaattc tacaaggaat tacatcaaaa tgatgaagtt aagatcaaca agtacagttc
     4501 cgcgaatatc aaggtgttga agcaagaaaa gaaggacctt tatattcgca attacatcgc
     4561 ccacttcaat tatattcctc acgccgagat ctcactgctg gaagtccttg aaaatttgcg
     4621 taaattgctg tcctacgatc gcaaactgaa aaatgccgta atgaaatcag tagttgatat
     4681 ccttaaggag tatggttttg tagccacatt caaaatcggg gcggacaaga agatcggtat
     4741 tcagacactg gagagcgaaa aaatcgtgca tcttaagaat cttaagaaga agaagttaat
     4801 gactgaccgc aattccgagg aactttgcaa attggtgaag attatgtttg aatacaaaat
     4861 ggaagagaaa aagtctgaaa actaataaat ccctcggtac caaagacgaa caataagacg
     4921 ctgaaaagcg tcttttttcg ttttggtccg ctgagcagtt acagagatgt tacgaaccac
     4981 tagtgcactg cagtacacct ttggtcgaaa aaaaaagccc gcactgtcag gtgcgggctt
     5041 ttttctgtgt ttccaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     5101 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     5161 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     5221 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     5281 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     5341 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
     5401 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     5461 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     5521 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     5581 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     5641 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     5701 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     5761 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     5821 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     5881 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaagc gctagcggag
     5941 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     6001 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     6061 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     6121 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     6181 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     6241 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6301 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     6361 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     6421 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     6481 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     6541 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     6601 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     6661 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     6721 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     6781 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     6841 tttcagccaa aaaacttaag accgccggtc ttgtccacta ccttgcagta atgcggtgga
     6901 caggatcggc ggttttcttt tctcttctca accctgaatt cgca
//
