LOCUS       Exported                3754 bp ds-DNA     circular SYN 09-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..135
                     /organism="Escherichia virus T4"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:10665"
     source          1146..2446
                     /organism="gbBA067_BBR1_mob_recoded"
     misc_feature    1..75
                     /label=pBA573_UP_Hom
     primer_bind     complement(1..55)
                     /label=oBA1762_T4Soc_DN52_R
     misc_feature    1..52
                     /label=T4_Soc_UP52
     gene            1..20
                     /gene="segF"
                     /gene_synonym="69"
                     /locus_tag="T4p030"
                     /label=segF
                     /db_xref="GeneID:1258803"
     CDS             1..20
                     /codon_start=1
                     /transl_table=11
                     /gene="segF"
                     /gene_synonym="69"
                     /locus_tag="T4p030"
                     /product="SegF homing endonuclease"
                     /label=segF
                     /note="site-specific endonuclease; relative of mobile 
                     endonucleases of filamentous fungi; GIY-YIG family"
                     /db_xref="GeneID:1258803"
                     /protein_id="NP_049645.1"
                     /translation="RKEDV"
     primer_bind     52..85
                     /label=oBA1769_T4Soc_OneCODON_F
     primer_bind     52..85
                     /label=oBA1771_T4Soc_SEED_F
     misc_feature    52..55
                     /label=4bp
     gene            53..135
                     /gene="soc"
                     /locus_tag="T4p029"
                     /label=soc
                     /db_xref="GeneID:1258783"
     CDS             53..135
                     /codon_start=1
                     /transl_table=11
                     /gene="soc"
                     /locus_tag="T4p029"
                     /product="Soc small outer capsid protein"
                     /label=soc
                     /note="small outer capsid head accessory protein, found on
                     T4 and a few related phages"
                     /db_xref="GeneID:1258783"
                     /protein_id="NP_049644.1"
                     /translation="MASTRGYVNIKTFEQKLDGNKKIEGKE"
     misc_feature    complement(53..83)
                     /label=T4_soc_CDS_1
     primer_bind     complement(56..89)
                     /label=oBA1770_T4Soc_OneCODON_R
     primer_bind     complement(56..89)
                     /label=oBA1772_T4Soc_SEED_R
     misc_feature    68..75
                     /label=SEED
     variation       68..70
                     /label=GGT-> GGC
     variation       71..73
                     /label=TAT-> TAC
     misc_feature    74..76
                     /label=GTT-> GTG
     misc_feature    84..135
                     /label=T4_Soc_DN52
     primer_bind     86..135
                     /label=oBA1763_T4Soc_UP52_F
     misc_feature    86..89
                     /label=4bp
     primer_bind     complement(90..139)
                     /label=oBA1764_T4Soc_DN52_R
     misc_feature    complement(95..125)
                     /label=T4_soc_CDS_2
     misc_feature    136..3754
                     /label=pBA707_p4
     primer_bind     136..184
                     /label=oBA1724_kanR_part4_gib_BBR1mob_R
     misc_feature    136..154
                     /label=Pt4_common_region_6>4
     primer_bind     136..151
                     /label=oBA1650_pBFC0924_Gib_F
     misc_feature    136..139
                     /label=6>4 OH
     misc_feature    complement(140..143)
                     /label=SMRT Gate OH Right
     misc_feature    complement(148..154)
                     /label=AarI
     terminator      complement(155..181)
                     /label=KanR Terminator
     CDS             complement(182..997)
                     /label=KanR
     primer_bind     complement(266..293)
                     /label=oBFC0064
     promoter        complement(998..1145)
                     /label=KanR Promoter
     primer_bind     complement(1120..1179)
                     /label=oBA1723_kanR_part4_gib_BBR1mob_F
     misc_feature    1146..2446
                     /label=gbBA067
     misc_feature    complement(1146..1185)
                     /label=post-Mob from pFAB5526
     primer_bind     1146..1167
                     /label=oBA1725_gbBA067_BBR1mob_R
     CDS             complement(1186..2184)
                     /codon_start=1
                     /label=pFAB5526_MOB_recoded
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLG"
     misc_feature    complement(2185..2446)
                     /label=pre-MOB from pFAB5526
     misc_feature    2252..2303
                     /label=oriT
     misc_feature    2314..2334
                     /label=ACTUAL Sequence
     primer_bind     complement(2413..2446)
                     /label=oBA1726_gbBA067_BBR1mob_F
     primer_bind     2417..2460
                     /label=oBA1727_BBR1ori_mob_R
     rep_origin      2447..3729
                     /direction=RIGHT
                     /label=BBR1 ori
     misc_feature    2447..3729
                     /label=From pBBR1 backbone
     misc_feature    2447..3729
                     /label=from pBFC0924
     CDS             3021..3683
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     complement(3179..3207)
                     /label=oBFC1872
     primer_bind     complement(3188..3207)
                     /label=oBFC1871
     primer_bind     3201..3225
                     /label=oBFC1870
     misc_feature    3203..3206
                     /label=BsaI OH
     misc_feature    3203
                     /label=silent c>t to remove BbsI
     primer_bind     complement(3705..3754)
                     /label=oBA1728_BBR1ori_mob_F
     misc_feature    3728..3729
                     /label=BsaI OH
     misc_feature    3730..3754
                     /label=Pt4_common_region_4>5
     misc_feature    3730..3736
                     /label=AarI
     primer_bind     complement(3736..3754)
                     /label=oBA1651_pBFC0924_Gib_R
     misc_feature    3741..3744
                     /label=SMRT Gate OH Left
     misc_feature    3745..3746
                     /label=spacer in case extra trimming during AarI GG
     misc_feature    3747..3750
                     /label=pBBR1 Backbone Index (GACT)
     primer_bind     join(3751..3754,1..51)
                     /label=oBA1761_T4Soc_UP52_F
     misc_feature    3751..3754
                     /label=4>5 OH
                     /label=4b(3)
     misc_feature    3751..3754
                     /label=BsaI OH
ORIGIN
        1 tcagaaaaga agatgtataa ataatcatgt aatttaaata aaggagaatt acatggctag
       61 tactcgcggc tacgtgaata tcaaaacatt tgagcagaaa ttagatggaa ataagaaaat
      121 tgaaggaaag gaaataggag gactttggca ggtggtgtta caaccaatta accaattctg
      181 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      241 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      301 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      361 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      421 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
      481 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
      541 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
      601 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
      661 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
      721 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
      781 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
      841 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
      901 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
      961 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     1021 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     1081 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     1141 atcagcttgc gttttccctt gtccagatag cccagtagct gacatttagc ctaaatgaca
     1201 tttctccgga aagtgcgcac ggccacgctc ttgttgacgt tgagcttgct tttgacgttg
     1261 aacctcgcgt tgctcggtta ataatttctc accaaccgct ttaataatgg ccgccgcttt
     1321 cgcttgcatg tcacggttca gaggaccgag tgcatctaat accggtttca aacgttcgcg
     1381 taaatcgcgc gcagtttcct gtgcctggtc tgctttctta cgcatttcgc gggcgccggc
     1441 agccgcttgc agtgctggtt cgtaaccttg acggactgcc ttagttaagc gatccgcgac
     1501 tgcctcaggg gtttccacac gtttagaaat gcctaatttc tccgccagac cttgtggagc
     1561 gtaagcacgg ggttccacgg cctgaggcga aatcgtaaca tgtcctacag gcgggcgttc
     1621 aagcgcttca taaaatgctt ggatacgagt atggcgcgct ttactccctt caatcccacg
     1681 ctgaaggccc aggtccgcaa ccgcagcagc gaaggtcgtt tgatcacgtg tcatttgggc
     1741 cttattacca ataaattctt tagcactcaa gcgaccatct tgggtcaagg ggactacaaa
     1801 ggccgtcata tgtggggatg tctcatcgcg atgaatcgaa gctgttacaa tacggtcagc
     1861 gccatattta tcggctaacc atttatgggc tttctcaaag aatgctgctt gttgctcctg
     1921 tgacgcgctt ttccaccact cgggcgaagc ggtcatcaca tattctacgg caagtactgc
     1981 atctttacgg cgcttttcag gcaacagttc acgcaaacga cccatggcct cgtccgtact
     2041 actcgcggcc caatgttcat tttccggagt acgagatgca tccgcattcg gtgtttcacg
     2101 ttcacgatac gcatgtttca gcgacgcagc aacattaccc atcttggcga gtttcttaca
     2161 acgcataatt gcataagcgg ccatgcctgc ccctcccttt tggtgtccaa ccggctcgac
     2221 gggggcagcg caaggcggtg cctccggcgg gccactcaat gcttgagtat actcactaga
     2281 ctttgcttcg caaagtcgtg accgcctacg gcggctgcgc ctgcccacga gcttgaccac
     2341 agggattgcc caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg
     2401 cctgcggcct tgccctatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg
     2461 gcctgcgcgc ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag
     2521 cgaccgcgca gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg
     2581 gcagtcgaag ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg
     2641 gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc
     2701 cccggagggg ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc
     2761 aggggtgccc ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa
     2821 aaatcaacaa cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag
     2881 ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt
     2941 gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc
     3001 cctaccgcat ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga
     3061 acaagcccgg tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta
     3121 ttgcgaggaa acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc
     3181 agaacgccgt ggtggtcagc caaaagacac tttccaagct catcggacgt tctttgcgga
     3241 cggtccaata cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca
     3301 acggccccgg caccgtgttg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc
     3361 gcgaccagtt gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg
     3421 acgaatcgct gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc
     3481 agcaactacc gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg
     3541 aaccagacct gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc
     3601 tgccgatgcc cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac
     3661 gggtcacgct gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc
     3721 cagactatcc acctgcccga accagagact tatc
//
