LOCUS       pAP05                   4944 bp    DNA     circular SYN 09-MAY-2022
DEFINITION  YGFP under Pbind with  05_EL222.
ACCESSION   JX560343
KEYWORDS    .
SOURCE      Cloning vector pSEVA531
  ORGANISM  Cloning vector pSEVA531
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de, las, Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R., de and
            Lorenzo,V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent
            platform for analysis and deployment of complex prokaryotic
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de, las, Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R., de and
            Lorenzo,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid,
            Madrid 28049, Spain
COMMENT     ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     misc_feature    1..8
                     /Recognition_pattern="TTAAT^TAA"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     primer_bind     9..>30
                     /note="R24"
     misc_feature    complement(10..30)
                     /created_by="primer3"
                     /annotation_group="1464728312013310000a2781707-4dc7-486e-8
                     6c9-02cef1787ee8"
                     /%GC="42.9"
                     /Tm="55.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTGTGTGAAATTGTTATCCGC"
                     /label="30 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(10..30)
                     /Sequence="CTGTGTGAAATTGTTATCCGC"
                     /Hairpin_Tm="None"
                     /Tm="55.9"
                     /Self_Dimer_Tm="None"
                     /%GC="42.9"
                     /created_by="primer3"
                     /label="PSEVRI"
     misc_feature    31..34
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     motif           35..52
                     /created_by="User"
                     /modified_by="User"
                     /label="EL222 binding region"
     regulatory      35..87
                     /created_by="User"
                     /modified_by="User"
                     /label="EL222 binding site"
     motif           53..58
                     /created_by="User"
                     /label="-35"
     misc_feature    complement(61..87)
                     /created_by="primer3"
                     /annotation_group="82864832631847c7924f3-b4af-4569-bb6c-c5
                     3a602b5954"
                     /%GC="25.9"
                     /Tm="55.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTTATTCGACTATAACAAACCATTTTC"
                     /label="83 R"
                     /note="Geneious type: primer_bind_reverse"
     motif           74..79
                     /created_by="User"
                     /label="-10"
     Insulator       88..102
                     /created_by="User"
                     /modified_by="User"
                     /label="Insulator "
     misc_feature    complement(88..102)
                     /created_by="primer3"
                     /annotation_group="1356074501976748d4d8bc11-3173-420c-8dc4
                     -ef890b711a0f"
                     /%GC="33.3"
                     /Tm="40.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AATGAATTCTGTGTG"
                     /label="98 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    94..99
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     RBS             103..115
                     /created_by="User"
                     /label="RBSD"
     Insulator       116..121
                     /created_by="User"
     CDS             122..838
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
                     /modified_by="User"
                     /label="sfgfp"
     source          <122..>838
                     /organism="pSR59.4"
                     /mol_type="other DNA"
     primer_bind     122..140
                     /created_by="primer3"
                     /annotation_group="8680416279558fa244962-6897-4c5f-ad85-e7
                     4d47a9e08a"
                     /%GC="52.6"
                     /Tm="58.6"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGCGTAAAGGCGAAGAGC"
                     /label="118 F"
     misc_feature    283..288
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    302..331
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
                     /label="Y66 (EGFP)"
     misc_feature    350..355
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(626..642)
                     /Sequence="CCATCCTCCACGTTGTG"
                     /Hairpin_Tm="None"
                     /Tm="54.7"
                     /Self_Dimer_Tm="None"
                     /%GC="58.8"
                     /created_by="primer3"
                     /label="spiece1r"
     misc_feature    770..775
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     misc_feature    828..833
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="BsrGI"
                     /note="Geneious type: restriction site"
     primer_bind     843..861
                     /created_by="primer3"
                     /annotation_group="1852208263494476543660a8-b56a-4978-bc21
                     -c5c332ead226"
                     /%GC="57.9"
                     /Tm="55.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.7"
                     /Sequence="GGGATCCTCTAGAGTCGAC"
                     /label="76 F"
     primer_bind     843..861
                     /Sequence="GGGATCCTCTAGAGTCGAC"
                     /Hairpin_Tm="None"
                     /Tm="55.4"
                     /Self_Dimer_Tm="7.7"
                     /%GC="57.9"
                     /created_by="primer3"
                     /label="PSEVFI"
     misc_feature    844..849
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    850..855
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    862..867
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    874..879
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    880..887
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(888..911)
                     /note="F24"
     primer_bind     897..911
                     /created_by="primer3"
                     /annotation_group="1464728234526319000659a1140-3f36-442c-9
                     943-d22dd699f388"
                     /%GC="66.7"
                     /Tm="54.0"
                     /Hairpin_Tm="34.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGAAAACCCTGGCG"
                     /label="130 F"
     misc_feature    912..917
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     regulatory      918..1020
                     /regulatory_class="terminator"
                     /note="T0"
     misc_feature    1047..1054
                     /Recognition_pattern="ATTT^AAAT"
                     /annotation_group="ATTTAAAT"
                     /label="SwaI"
                     /note="Geneious type: restriction site"
     primer_bind     1053..1076
                     /Sequence="ATTTGACAGCTTATCATCGATAAA"
                     /Hairpin_Tm="34.6"
                     /Tm="54.8"
                     /Self_Dimer_Tm="1.2"
                     /%GC="29.2"
                     /created_by="primer3"
                     /label="spiece2f"
     misc_feature    complement(1076..1097)
                     /created_by="primer3"
                     /annotation_group="18524938426575953ffa7088-00b3-4f13-8e9e
                     -7b701b865986"
                     /%GC="36.4"
                     /Tm="55.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATAAACTACCGCATTACAGT"
                     /label="330 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1076..1097)
                     /created_by="User"
                     /Sequence="TGATAAACTACCGCATTACAGT"
                     /label="PSEVRII"
                     /note="Geneious type: primer_bind_reverse"
     primer          1102..1131
                     /created_by="User"
                     /label="J23105"
     source          <1102..>1842
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     source          join(<1102..>1131,<1102..>1131)
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     Insulator       1132..1146
                     /created_by="User"
                     /modified_by="User"
     misc_feature    1138..1143
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     RBS             1147..1158
                     /created_by="User"
                     /label="RBS34"
     Insulator       1159..1164
                     /created_by="User"
                     /modified_by="User"
                     /label="insulator2"
     gene            1165..1842
                     /created_by="User"
                     /label="EL222"
     primer_bind     1165..1186
                     /created_by="primer3"
                     /annotation_group="306772218253758353949d8-8327-41ef-8c96-
                     2757c38285f2"
                     /%GC="40.9"
                     /Tm="55.6"
                     /Hairpin_Tm="47.6"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGTTGGATATGGGACAAGATC"
                     /label="1 F"
     misc_feature    complement(1825..1842)
                     /created_by="primer3"
                     /annotation_group="912342675136928e0094ee1-e95f-4582-bcca-
                     875cc712affa"
                     /%GC="55.6"
                     /Tm="56.8"
                     /Hairpin_Tm="35.6"
                     /Self_Dimer_Tm="0.2"
                     /Sequence="TCAGATTCCGGCTTCGAC"
                     /label="678 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1847..1868
                     /created_by="primer3"
                     /annotation_group="185257830681239702423394-8d52-4bf1-8ac1
                     -fc8d1def6352"
                     /%GC="36.4"
                     /Tm="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.7"
                     /Sequence="TATGAAATCTAACAATGCGCTC"
                     /label="363 F"
     primer_bind     join(1847..1868,1847..1868)
                     /Sequence="TATGAAATCTAACAATGCGCTC"
                     /Hairpin_Tm="None"
                     /Tm="55.0"
                     /Self_Dimer_Tm="9.7"
                     /%GC="36.4"
                     /created_by="primer3"
                     /label="PSEVFII"
     primer_bind     complement(1868..1884)
                     /Sequence="TGCCGAGGATGACGATG"
                     /Hairpin_Tm="None"
                     /Tm="56.3"
                     /Self_Dimer_Tm="None"
                     /%GC="58.8"
                     /created_by="primer3"
                     /label="spiece2r"
     CDS             1920..3038
                     /gene="tetA"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux transporter"
                     /protein_id="AFV59807.1"
                     /db_xref="GI:410073724"
                     /translation="MPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSD
                     RFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDG
                     EDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQES
                     HKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFR
                     WSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRG
                     WMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAI
                     YAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            1920..3038
                     /gene="tetA"
     misc_feature    1991..1996
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    3039..3048
                     /Recognition_pattern="GACNN^NNGTC"
                     /annotation_group="GACNNNNGTC"
                     /label="PshAI"
                     /note="Geneious type: restriction site"
     misc_feature    3049..3294
                     /note="oriT"
     primer_bind     3275..3289
                     /created_by="primer3"
                     /annotation_group="1466438986464120000a739800f-3714-4d39-8
                     8dc-9174f0f746b6"
                     /%GC="73.3"
                     /Tm="56.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.8"
                     /Sequence="GCTCTGCGAGGCTGG"
                     /label="1,791 F"
     misc_feature    3295..3302
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI"
                     /note="Geneious type: restriction site"
     CDS             complement(3303..3965)
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AFV59808.1"
                     /db_xref="GI:410073725"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            complement(3303..3965)
                     /gene="rep"
     primer_bind     3303..3315
                     /created_by="primer3"
                     /annotation_group="1464795714538271000c0396fd5-97a1-4f32-b
                     9af-739427d4aec3"
                     /%GC="84.6"
                     /Tm="56.4"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="24.5"
                     /Sequence="CTACCGGCGCGGC"
                     /label="1,819 F"
     misc_feature    complement(4804..4819)
                     /created_by="primer3"
                     /annotation_group="14664392394251400001a54adc0-9179-42f5-a
                     e63-c982159983f3"
                     /%GC="62.5"
                     /Tm="55.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TACGGGCTTGCTCTCC"
                     /label="3,335 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4825..4832
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI"
                     /note="Geneious type: restriction site"
     regulatory      4839..4943
                     /regulatory_class="terminator"
                     /note="T1"
     primer_bind     4898..4915
                     /Sequence="CGAAAGGCCCAGTCTTTC"
                     /Hairpin_Tm="39.0"
                     /Tm="55.7"
                     /Self_Dimer_Tm="10.9"
                     /%GC="55.6"
                     /created_by="primer3"
                     /label="spiece1f"
ORIGIN      
        1 ttaattaaag cggataacaa tttcacacag agccggtagc ctttagtcca tgttagcgaa
       61 gaaaatggtt tgttatagtc gaataaacac acagaattca ttaagaagga gatatggtac
      121 catgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg
      181 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg
      241 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct
      301 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca
      361 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt
      421 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt
      481 aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa
      541 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg
      601 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga
      661 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta
      721 tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct
      781 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatgaac
      841 acgggatcct ctagagtcga cctgcaggca tgcaagcttg cggccgcgtc gtgactggga
      901 aaaccctggc gactagtctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      961 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccag
     1021 gggtccccaa taattacgat ttacgtattt aaatttgaca gcttatcatc gataaactgt
     1081 aatgcggtag tttatcaagg aggctagctc agtcctaggt actatgctag ccacacagaa
     1141 ttcattaaag aggagaaagg taccatgttg gatatgggac aagatcggcc gatcgatgga
     1201 agtggggcac ccggggcaga cgacacacgc gttgaggtgc aaccgccggc gcagtgggtc
     1261 ctcgacctga tcgaggccag cccgatcgca tcggtcgtgt ccgatccgcg tctcgccgac
     1321 aatccgctga tcgccatcaa ccaggccttc accgacctga ccggctattc cgaagaagaa
     1381 tgcgtcggcc gcaattgccg attcctggca ggttccggca ccgagccgtg gctgaccgac
     1441 aagatccgcc aaggcgtgcg cgagcacaag ccggtgctgg tcgagatcct gaactacaag
     1501 aaggacggca cgccgttccg caatgccgtg ctcgttgcac cgatctacga tgacgacgac
     1561 gagcttctct atttcctcgg cagccaggtc gaagtcgacg acgaccagcc caacatgggc
     1621 atggcgcgcc gcgaacgcgc cgcggaaatg ctcaagacgc tgtcgccgcg ccagctcgag
     1681 gttacgacgc tggtggcatc gggcttgcgc aacaaggaag tggcggcccg gctcggcctg
     1741 tcggagaaaa ccgtcaagat gcaccgcggg ctggtgatgg aaaagctcaa cctgaagacc
     1801 agcgccgatc tggtgcgcat tgccgtcgaa gccggaatct gacttctatg aaatctaaca
     1861 atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta
     1921 tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact
     1981 atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag
     2041 cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta
     2101 tcgactacgc gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg
     2161 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     2221 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     2281 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cctgcacgca ccattccttg
     2341 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     2401 ataagggaga gcgccgtccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     2461 gggcgcgggg catgaccatt gtggccgcac ttatgactgt cttctttatc atgcaactcg
     2521 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     2581 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     2641 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     2701 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     2761 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     2821 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     2881 gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
     2941 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
     3001 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaga caattgtcct tttccgctgc
     3061 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     3121 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     3181 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     3241 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     3301 ccctaccggc gcggcagcgt tacccgtgtc ggcggctcca acggctcgcc atcgtccaga
     3361 aaacacggct catcgggcat cggcaggcgc tgctgcccgc gccgttccca ttcctccgtt
     3421 tcggtcaagg ctggcaggtc tggttccatg cccggaatgc cgggctggct gggcggctcc
     3481 tcgccggggc cggtcggtag ttgctgctcg cccggataca gggtcgggat gcggcgcagg
     3541 tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac ggcggcactg
     3601 aacaccgaca ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc attgaccacg
     3661 taggccgaca cggtgccggg gccgttgagc ttcacgacgg agatccagcg ctcggccacc
     3721 aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt ggaaagtgtc
     3781 ttctggctga ccaccacggc gttctggtgg cccatctgcg ccacgaggtg atgcagcagc
     3841 attgccgccg tgggtttcct cgcaataagc ccggcccacg cctcatgcgc tttgcgttcc
     3901 gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc cgatttctct ggactgcgtg
     3961 gccatgctta tctccatgcg gtaggggtgc cgcacggttg cggcaccatg cgcaatcagc
     4021 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
     4081 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
     4141 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
     4201 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
     4261 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
     4321 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
     4381 cgggcgggct tcgcccttcg actgccccca ctcgcatagg cttgggtcgt tccaggcgcg
     4441 tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga cccgccttcc acttggtgtc
     4501 caaccggcaa gcgaagcgcg caggccgcag gccggaggct tttccccaga gaaaattaaa
     4561 aaaattgatg gggcaaggcc gcaggccgcg cagttggagc cggtgggtat gtggtcgaag
     4621 gctgggtagc cggtgggcaa tccctgtggt caagctcgtg ggcaggcgca gcctgtccat
     4681 cagcttgtcc agcagggttg tccacgggcc gagcgaagcg agccagccgg tggccgctcg
     4741 cggccatcgt ccacatatcc acgggctggc aagggagcgc agcgaccgcg cagggcgaag
     4801 cccggagagc aagcccgtag ggggggcgcg cccagctgtc tagggcggcg gatttgtcct
     4861 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     4921 agcctttcgt tttatttgat gcct
//
