LOCUS       pAP14                   8399 bp    DNA     circular SYN 09-MAY-2022
DEFINITION  Pbind_rbs34 wgaAB .
ACCESSION   JX560343
KEYWORDS    .
SOURCE      Cloning vector pSEVA531
  ORGANISM  Cloning vector pSEVA531
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de, las, Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R., de and
            Lorenzo,V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent
            platform for analysis and deployment of complex prokaryotic
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de, las, Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R., de and
            Lorenzo,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid,
            Madrid 28049, Spain
COMMENT     ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     misc_feature    1..8
                     /Recognition_pattern="TTAAT^TAA"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     misc_feature    35..38
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          35..61
                     /created_by="User"
                     /label="F_GG_Pbind"
     unsure          39..56
                     /created_by="User"
                     /label="El-222 binding site"
     promoter        39..91
                     /created_by="User"
                     /label="Pbind "
     primer          39..124
                     /created_by="User"
                     /label="Pbind_rbs34_overlap "
     misc_feature    92..97
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          98..145
                     /created_by="User"
                     /label="Rbs34_wgaAB overlap "
     primer          98..124
                     /created_by="User"
                     /label="rbs34"
     misc_feature    99..124
                     /Original_Bases="GTACTAGCGAACGTTTAAGAAGGAGATATACATTCTAGA"
                     /label="GTACTAGCGAACGTTTAAGAAGGAGATATACATTCTAGA"
                     /note="Geneious type: Editing History Replacement"
     CDS             <125..1597
                     /gene="wgaA"
                     /locus_tag="SM_b21319"
                     /old_locus_tag="SMb21319"
                     /note="Hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="putative membrane protein WgaA (formerly ExpA1)"
                     /protein_id="CAC49295.1"
                     /db_xref="GI:15140781"
                     /db_xref="EnsemblGenomes-Gn:SM_b21319"
                     /db_xref="EnsemblGenomes-Tr:CAC49295"
                     /db_xref="UniProtKB/TrEMBL:Q7ANQ0"
                     /translation="MSSNVRQSPAKRRLVVASGLDRENFEGSDIEHLVHFLEPDAALR
                     FPLHNAFPLIALAFSDEGEADLREGLASINALGTIPEIPLQRIDADGKAESLALVRSL
                     VEGGVGRLSRFTSSVTAELAILRRERETLLENYRALEDAFQARNWEPVAEIFAHDPYA
                     DPKDEGIGQLLATGCVEQLLPVSSLGVAGVALHLHSVPRDGGELVVMLGYVENGEGVA
                     EWTVPYAQLAANWNFLALPRACGGAARTLRLRISTTGTETVGLSLGYPIASERYTARS
                     ETPHPDLDLRPLAFRVFTGLPGVKPTRMPNMIAPTALLEGHFIEDYRLAVELLSQIVD
                     VSVTPVVPEFQTVRFLEHENAVVCHPLPSGISAGTIGRAVEPGTISFSASAIIDHPKG
                     APAAVSFLLAPANSNARSEVAELARKGAAKPSAFFSGWREVTSEQAVNVNFQLDKPVR
                     EPMDLMILSRAVTDTVDFSWLKVSGFRLVKQSAGASNVRK"
     source          <125..>4262
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     primer_bind     742..764
                     /Sequence="CATGCTGGGTTATGTCGAGAATG"
                     /Hairpin_Tm="None"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="47.8"
                     /modified_by="User"
                     /label="wgaAB seq2"
     primer_bind     1575..1590
                     /Sequence="GGGCGTCCAATGTCCG"
                     /Hairpin_Tm="None"
                     /Tm="57.6"
                     /Self_Dimer_Tm="None"
                     /%GC="68.8"
                     /modified_by="User"
                     /label="wgaAB seq3"
     CDS             1584..>4265
                     /gene="wgaB"
                     /locus_tag="SM_b21320"
                     /old_locus_tag="SMb21320"
                     /note="Hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional
                     glycosyltransferase,forming beta-glycosyl and
                     alpha-glycosyl linkages protein WgaB (formerly ExpA23)"
                     /protein_id="CAC49296.1"
                     /db_xref="GI:15140782"
                     /db_xref="EnsemblGenomes-Gn:SM_b21320"
                     /db_xref="EnsemblGenomes-Tr:CAC49296"
                     /db_xref="GOA:Q92V32"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="InterPro:IPR028098"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q92V32"
                     /translation="MSGNKENSDLIVVAMPLYGHAALVLEAIESVLASKLAGCRVAVV
                     VSVDGDPRQETFDQLLLYAAAHPAVHVLFGANAGPGGARNRAIDYVLANLPEAEAVYF
                     LDADNRVLPGTIETLYRQLRASGCGWIYTNIDTFSVSWRAHYGNRYSRLLHCITDNIC
                     DTGSMISIDVFRAGVRFDDDRQNGFEDWEFWLSCIEHGFVGAPCHDTTFEYRLRAESR
                     FKEANRDRAASVSFLRKRHRALFQRPMLVDFEHEECPRYLFARTEDAAISFFTDPTKT
                     PRRLRLDDIIPAFWASIGEPDNVHFPPFLIAGSGATLDLLLRSRMVPNVLSHLERLSE
                     KANVVFVQLGNDAAQRKIEPVFLEAGAQHNAPADLIFLSTSLVRDVIHNKALDWFASI
                     GNQQVWPTSAILKVRFPFPRSLPRRSLITPQQVMINCVNAIATSPLRRTAGKRWTWRP
                     ARLVPYSDLHKALRHEIGGSPVLPLGHSEGSRKTAALLVPNASFGGAEKVVYAASREL
                     KAAGYETHLFVLGTSRMDVIDEFDQSFDYIHFWDQGIPAWGGSGSFLGQDFIAEGHDV
                     DWAALKGQLSGFDLVINNHVMAVHPLIARLRSEGTRTACYLHVVDNTSFKRPAGQPFA
                     AIAHEHCYDAFLTCSEQLKIYLHSFGVPYEKIFAVPNGASFSVPPKVLAEVLSVRRIE
                     RKDDRLRVLYMGRLDQQKGIDRLAAAFAELRASRVPFDARAIGGEILADATLSWTDRL
                     KDLGVEVRPPVFASKDLIKALGWADVLLMPSRWEGAPLMIAEAQQLGCVPIATAVGAV
                     DELITNGEDGILIEAAADPQVVRDMAKAIEEVANNRQMLAPLMEGCLRTAARRSWTSS
                     FSEFLGWCDRSVNNSSLSRATVIRGREASNPGVAAVG"
     primer_bind     2369..2386
                     /Sequence="GGAAGATGCCGCCATTTC"
                     /Hairpin_Tm="None"
                     /Tm="56.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /modified_by="User"
                     /label="wgaAB seq4"
     primer_bind     3173..3193
                     /Sequence="GTTCGATCAGAGCTTCGACTA"
                     /Hairpin_Tm="None"
                     /Tm="57.3"
                     /Self_Dimer_Tm="4.8"
                     /%GC="47.6"
                     /modified_by="User"
                     /label="wgaAB seq5"
     misc_feature    4263..4326
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     gene            <4263..4265
                     /gene="wgaB"
                     /locus_tag="SM_b21320"
                     /old_locus_tag="SMb21320"
     misc_feature    complement(4270..4285)
                     /created_by="primer3"
                     /annotation_group="967296264008051bd2f6b9c-3281-4e2c-a450-
                     903d2b914b7a"
                     /%GC="56.3"
                     /Tm="54.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.1"
                     /Sequence="TTCTTGAAACCGGCGG"
                     /label="5,006 R"
                     /note="Geneious type: primer_bind_reverse"
     GG_intsert      4286..4289
                     /created_by="User"
                     /label="GG ins"
     source          join(<4286..>4326,<4286..>4326)
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     primer_bind     4290..4308
                     /created_by="primer3"
                     /annotation_group="1852208263494476543660a8-b56a-4978-bc21
                     -c5c332ead226"
                     /%GC="57.9"
                     /Tm="55.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.7"
                     /Sequence="GGGATCCTCTAGAGTCGAC"
                     /label="76 F"
     primer_bind     4290..4308
                     /Sequence="GGGATCCTCTAGAGTCGAC"
                     /Hairpin_Tm="None"
                     /Tm="55.4"
                     /Self_Dimer_Tm="7.7"
                     /%GC="57.9"
                     /created_by="primer3"
                     /label="PSEVFI"
     misc_feature    4291..4296
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    4297..4302
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    4309..4314
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    4321..4326
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     primer_bind     4327..4340
                     /created_by="primer3"
                     /annotation_group="10490641588861065718a1d6-5ca9-4b2f-ab35
                     -be17c1f2eb02"
                     /%GC="85.7"
                     /Tm="61.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="37.0"
                     /Sequence="GCGGCCGCGTCGTG"
                     /label="4,269 F"
     misc_feature    4327..4334
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    4359..4364
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     regulatory      4365..4467
                     /regulatory_class="terminator"
                     /note="T0"
     misc_feature    4494..4501
                     /Recognition_pattern="ATTT^AAAT"
                     /annotation_group="ATTTAAAT"
                     /label="SwaI"
                     /note="Geneious type: restriction site"
     primer_bind     4500..4523
                     /Sequence="ATTTGACAGCTTATCATCGATAAA"
                     /Hairpin_Tm="34.6"
                     /Tm="54.8"
                     /Self_Dimer_Tm="1.2"
                     /%GC="29.2"
                     /created_by="primer3"
                     /label="spiece2f"
     misc_feature    complement(4523..4544)
                     /created_by="primer3"
                     /annotation_group="18524938426575953ffa7088-00b3-4f13-8e9e
                     -7b701b865986"
                     /%GC="36.4"
                     /Tm="55.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATAAACTACCGCATTACAGT"
                     /label="330 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4523..4544)
                     /created_by="User"
                     /Sequence="TGATAAACTACCGCATTACAGT"
                     /label="PSEVRII"
                     /note="Geneious type: primer_bind_reverse"
     promoter        4545..4590
                     /created_by="User"
                     /label="LacIq"
     misc_feature    4545..4590
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          join(<4545..>4590,<4545..>4590)
                     /organism="Cloning vector pSEVA531"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246754"
     Insulator       4591..4605
                     /created_by="User"
                     /modified_by="User"
     misc_feature    4593..5301
                     /Original_Bases="GTTAAATTGCTAACGCAGTCAGGCACCGTG"
                     /label="GTTAAATTGCTAACGCAGTCAGGCACCGTG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4597..4602
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     RBS             4606..4617
                     /created_by="User"
                     /label="RBS34"
     Insulator       4618..4623
                     /created_by="User"
                     /modified_by="User"
                     /label="insulator2"
     gene            4624..5301
                     /created_by="User"
                     /label="EL222"
     primer_bind     4624..4645
                     /created_by="primer3"
                     /annotation_group="306772218253758353949d8-8327-41ef-8c96-
                     2757c38285f2"
                     /%GC="40.9"
                     /Tm="55.6"
                     /Hairpin_Tm="47.6"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGTTGGATATGGGACAAGATC"
                     /label="1 F"
     misc_feature    complement(5284..5301)
                     /created_by="primer3"
                     /annotation_group="912342675136928e0094ee1-e95f-4582-bcca-
                     875cc712affa"
                     /%GC="55.6"
                     /Tm="56.8"
                     /Hairpin_Tm="35.6"
                     /Self_Dimer_Tm="0.2"
                     /Sequence="TCAGATTCCGGCTTCGAC"
                     /label="678 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5302..5323
                     /created_by="primer3"
                     /annotation_group="185257830681239702423394-8d52-4bf1-8ac1
                     -fc8d1def6352"
                     /%GC="36.4"
                     /Tm="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.7"
                     /Sequence="TATGAAATCTAACAATGCGCTC"
                     /label="363 F"
     primer_bind     join(5302..5323,5302..5323)
                     /Sequence="TATGAAATCTAACAATGCGCTC"
                     /Hairpin_Tm="None"
                     /Tm="55.0"
                     /Self_Dimer_Tm="9.7"
                     /%GC="36.4"
                     /created_by="primer3"
                     /label="PSEVFII"
     primer_bind     complement(5323..5339)
                     /Sequence="TGCCGAGGATGACGATG"
                     /Hairpin_Tm="None"
                     /Tm="56.3"
                     /Self_Dimer_Tm="None"
                     /%GC="58.8"
                     /created_by="primer3"
                     /label="spiece2r"
     CDS             5375..6493
                     /gene="tetA"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux transporter"
                     /protein_id="AFV59807.1"
                     /db_xref="GI:410073724"
                     /translation="MPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSD
                     RFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDG
                     EDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQES
                     HKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFR
                     WSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRG
                     WMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAI
                     YAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            5375..6493
                     /gene="tetA"
     misc_feature    5446..5451
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    6494..6503
                     /Recognition_pattern="GACNN^NNGTC"
                     /annotation_group="GACNNNNGTC"
                     /label="PshAI"
                     /note="Geneious type: restriction site"
     misc_feature    6504..6749
                     /note="oriT"
     primer_bind     6730..6744
                     /created_by="primer3"
                     /annotation_group="1466438986464120000a739800f-3714-4d39-8
                     8dc-9174f0f746b6"
                     /%GC="73.3"
                     /Tm="56.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.8"
                     /Sequence="GCTCTGCGAGGCTGG"
                     /label="1,791 F"
     misc_feature    6750..6757
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI"
                     /note="Geneious type: restriction site"
     CDS             complement(6758..7420)
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AFV59808.1"
                     /db_xref="GI:410073725"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            complement(6758..7420)
                     /gene="rep"
     primer_bind     6758..6770
                     /created_by="primer3"
                     /annotation_group="1464795714538271000c0396fd5-97a1-4f32-b
                     9af-739427d4aec3"
                     /%GC="84.6"
                     /Tm="56.4"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="24.5"
                     /Sequence="CTACCGGCGCGGC"
                     /label="1,819 F"
     primer_bind     8213..8227
                     /created_by="primer3"
                     /annotation_group="15350431664363580008b3f6190-8d99-4075-a
                     3b0-87cd80281742"
                     /%GC="66.7"
                     /Tm="57.7"
                     /Hairpin_Tm="40.4"
                     /Self_Dimer_Tm="2.9"
                     /Sequence="TCCACGGGCTGGCAA"
                     /label="8,152 F"
     misc_feature    complement(8259..8274)
                     /created_by="primer3"
                     /annotation_group="14664392394251400001a54adc0-9179-42f5-a
                     e63-c982159983f3"
                     /%GC="62.5"
                     /Tm="55.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TACGGGCTTGCTCTCC"
                     /label="3,335 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    8280..8287
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI"
                     /note="Geneious type: restriction site"
     regulatory      8294..8398
                     /regulatory_class="terminator"
                     /note="T1"
     primer_bind     8353..8370
                     /Sequence="CGAAAGGCCCAGTCTTTC"
                     /Hairpin_Tm="39.0"
                     /Tm="55.7"
                     /Self_Dimer_Tm="10.9"
                     /%GC="55.6"
                     /created_by="primer3"
                     /label="spiece1f"
ORIGIN      
        1 ttaattaaag cggataacaa tttcacacag agccaggagg tagcctttag tccatgttag
       61 cgaagaaaat ggtttgttat agtcgaataa acacacagaa ttcattaaag aggagaaagg
      121 taccatgtct tctaacgtga ggcagtcgcc ggcgaagcga aggctggtcg tggcatccgg
      181 tctggaccgg gaaaatttcg aagggagcga catcgagcat ctggttcatt ttctcgaacc
      241 ggatgcagct ttgcgatttc ccctgcataa cgcctttcct cttatcgcgc ttgccttctc
      301 ggatgagggt gaagccgatc tgcgggaagg gctcgcgtcc atcaatgctc ttggtaccat
      361 ccccgagata ccgcttcagc ggatcgacgc ggacggtaaa gcggaaagcc ttgcgctcgt
      421 gcggtcgctg gtggagggag gagtgggtcg gctctcccgt ttcacgtcgt cggtaaccgc
      481 cgagctcgcc attctccggc gtgagcgaga aacgctgctc gaaaattacc gcgcgctgga
      541 ggatgccttt caggcccgca attgggaacc ggtcgcggag atattcgccc acgaccctta
      601 tgccgacccg aaggacgagg gcatcgggca gctccttgcg accggctgcg tcgagcagct
      661 tcttccggtc tctagtcttg gcgttgcggg ggtcgcgctg caccttcatt ccgtgcccag
      721 ggacggcggc gaactggtcg tcatgctggg ttatgtcgag aatggcgagg gcgtggccga
      781 atggaccgtg ccctatgcgc agctcgcggc aaactggaat ttcctcgccc tgccgcgcgc
      841 ttgcggcggc gccgcgagaa cgctgcggct tagaatctca acgaccggca cggagactgt
      901 agggctttca ctcggctatc cgatcgcgag cgagcggtac acagcccgct cggaaacccc
      961 gcatcccgat ctcgatctcc ggcccctggc gttccgcgtc tttacgggac tgcccggcgt
     1021 caaaccgacg cgcatgccga acatgatcgc tccgaccgcc ttgctcgaag gccacttcat
     1081 cgaggattat cgtctggcgg tcgaattgct tagtcagatc gtcgacgttt cggtcacgcc
     1141 ggtcgttccc gaattccaga ccgtccgctt cctcgagcat gagaacgcgg tcgtttgcca
     1201 cccgctgccg agtggaatct ccgccggcac gatcggccgc gcagtggaac ccggcaccat
     1261 ctccttctcc gcaagcgcga tcatcgatca cccgaagggc gcacccgcgg ccgtgagctt
     1321 cctgctcgct ccggcgaact ccaatgcgcg ctcggaagtg gcggaactcg cgcgcaaggg
     1381 tgctgccaag ccctcggcct tcttcagcgg ctggcgcgag gtcacctccg agcaggcggt
     1441 caacgtgaac tttcaactgg acaagcccgt gcgcgagccg atggacctca tgattctcag
     1501 ccgcgcggtg acggatacgg tcgatttctc ctggctcaag gtctccggct tccgattggt
     1561 aaagcagtcc gcaggggcgt ccaatgtccg gaaataagga aaactcggac ctgatcgtgg
     1621 tggcaatgcc gctttatggc cacgcagccc tcgtcctgga ggcgatcgag tccgtgctcg
     1681 cttcgaagct tgccgggtgc cgggtggcag tggtggtgtc cgtcgatggt gatccgcgac
     1741 aggagacatt cgatcagttg ctgctctatg cggcggccca tcctgcggtt catgtgcttt
     1801 tcggggccaa tgccgggccg ggcggggcgc gcaatcgcgc gatcgactac gtgctcgcga
     1861 atctgccgga agcagaggcc gtctattttc tcgatgccga caatcgcgtg ctgcccggga
     1921 ccatcgagac tctctaccgg caactgcgcg ccagcggctg cggctggatc tataccaata
     1981 tcgacacgtt ttcggtgagc tggcgcgccc attacggcaa tcgctactcg cggcttctgc
     2041 actgcatcac ggacaatatc tgcgacaccg gttcgatgat ctcgatcgac gttttccggg
     2101 cgggggtccg cttcgacgac gacaggcaga acggtttcga ggattgggag ttctggctgt
     2161 cctgcatcga gcacggcttc gtgggggcgc cgtgccacga cacgaccttc gaataccggc
     2221 tgagggcgga aagccgcttc aaggaggcga accgcgatcg cgccgcctcg gtgagcttcc
     2281 tcagaaagcg ccaccgggcg ctgttccagc ggccaatgct ggtcgatttc gagcacgagg
     2341 aatgcccgcg ctatcttttc gcacggacgg aagatgccgc catttcgttc tttacggatc
     2401 cgaccaagac gcccaggcgt cttcgcctcg acgatatcat tccagccttc tgggcgagca
     2461 tcggcgagcc tgacaacgtt cacttcccgc ccttcctgat cgccggaagc ggtgccacgc
     2521 tcgatctgct gctgcgctcc cggatggtgc cgaacgtgct ctcccatctg gagcgcctga
     2581 gcgagaaggc caacgtggtc ttcgtccagc tcggcaacga tgcggctcag cgcaagatcg
     2641 agccggtctt cctggaggcc ggcgcccagc acaacgcacc ggccgacctc atcttcctgt
     2701 ccacttcgct cgtgcgcgac gtcattcata acaaggcgct ggactggttt gcctccatcg
     2761 gcaaccagca ggtctggccg acatcggcaa ttctgaaagt ccgcttccct ttcccgagaa
     2821 gcctgccgcg ccgctcgctc atcacgcccc agcaggtgat gatcaattgc gtcaatgcca
     2881 tcgcgaccag tccgctgcgg cggaccgccg gcaaacgctg gacctggcgt ccggcacggc
     2941 tcgttcccta ttccgacctc cacaaggcgc ttcgccacga gatcggtggc tcgcccgtgt
     3001 tgccgctcgg tcatagcgag ggaagcagga agaccgcagc cctgcttgtt ccgaatgcat
     3061 ccttcggcgg tgccgagaag gtcgtatatg cggcctcgcg cgagctgaag gccgccggct
     3121 acgagaccca tctcttcgtg ctcggcacat ccaggatgga tgtgatcgac gagttcgatc
     3181 agagcttcga ctatattcac ttctgggacc aaggcattcc cgcctggggc ggctccggct
     3241 cgttcctggg gcaggacttc atcgccgagg gccacgacgt cgattgggct gcgctcaagg
     3301 gtcagctttc cggcttcgac ctcgtgatca acaatcacgt catggcggtg catccgctta
     3361 tcgcgcggtt acgctcggag ggaacgcgca ccgcctgcta ccttcacgtg gtcgacaata
     3421 cctccttcaa aaggcccgcc ggtcagccct tcgcggcgat tgcccacgag cattgctacg
     3481 atgccttcct gacctgctcg gagcagctca agatctatct gcacagcttc ggcgttccct
     3541 atgaaaagat cttcgccgtc cccaacggcg cgagcttctc cgtgccgccc aaggttctcg
     3601 cggaggtgct gtcggtcagg cgcatcgaac gcaaggacga tcggcttcgg gtcctctata
     3661 tgggtcggct cgatcagcag aaggggatcg accgtctcgc tgcggcgttc gccgaattgc
     3721 gggcttcgcg cgtgccattc gatgcccggg cgatcggcgg ggaaatcctc gcggatgcca
     3781 ccttgtcgtg gacggatcgc ctgaaggacc tcggcgtcga ggtgcgcccg ccggtgttcg
     3841 caagcaagga cctgatcaag gccctcggtt gggcggatgt ccttctgatg ccgtcgcgct
     3901 gggagggcgc tcccttgatg atcgccgagg cgcagcagct cggctgcgtc ccgatcgcga
     3961 cggcagtcgg tgccgtcgac gaactcataa ccaacggcga ggacggcatc ctcatcgagg
     4021 ccgccgccga tccccaggtg gtccgggaca tggcaaaggc catcgaggag gtggccaaca
     4081 accgccagat gctcgcgccc ctcatggagg ggtgcctgag aacggcggca cgccgctcat
     4141 ggacgtcctc cttttccgag ttcctcggct ggtgcgaccg ttccgtgaac aattcatcgc
     4201 tttcgcgcgc aacggtcatc cgtgggcgcg aagcatccaa tcccggagtt gcggccgtgg
     4261 gctgaggccc cgccggtttc aagaaacacg ggatcctcta gagtcgacct gcaggcatgc
     4321 aagcttgcgg ccgcgtcgtg actgggaaaa ccctggcgac tagtcttgga ctcctgttga
     4381 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
     4441 gggcgttttt tattggtgag aatccagggg tccccaataa ttacgattta cgtatttaaa
     4501 tttgacagct tatcatcgat aaactgtaat gcggtagttt atcacgaatg gtgcaaaacc
     4561 tttcgcggta tggcatgata gcgcccggaa cacacagaat tcattaaaga ggagaaaggt
     4621 accatgttgg atatgggaca agatcggccg atcgatggaa gtggggcacc cggggcagac
     4681 gacacacgcg ttgaggtgca accgccggcg cagtgggtcc tcgacctgat cgaggccagc
     4741 ccgatcgcat cggtcgtgtc cgatccgcgt ctcgccgaca atccgctgat cgccatcaac
     4801 caggccttca ccgacctgac cggctattcc gaagaagaat gcgtcggccg caattgccga
     4861 ttcctggcag gttccggcac cgagccgtgg ctgaccgaca agatccgcca aggcgtgcgc
     4921 gagcacaagc cggtgctggt cgagatcctg aactacaaga aggacggcac gccgttccgc
     4981 aatgccgtgc tcgttgcacc gatctacgat gacgacgacg agcttctcta tttcctcggc
     5041 agccaggtcg aagtcgacga cgaccagccc aacatgggca tggcgcgccg cgaacgcgcc
     5101 gcggaaatgc tcaagacgct gtcgccgcgc cagctcgagg ttacgacgct ggtggcatcg
     5161 ggcttgcgca acaaggaagt ggcggcccgg ctcggcctgt cggagaaaac cgtcaagatg
     5221 caccgcgggc tggtgatgga aaagctcaac ctgaagacca gcgccgatct ggtgcgcatt
     5281 gccgtcgaag ccggaatctg atatgaaatc taacaatgcg ctcatcgtca tcctcggcac
     5341 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
     5401 ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc
     5461 gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg
     5521 cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac
     5581 acccgtcctg tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     5641 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     5701 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     5761 gttgggcgcc atctccctgc acgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     5821 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gtccgatgcc
     5881 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ccattgtggc
     5941 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
     6001 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     6061 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     6121 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     6181 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     6241 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     6301 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct
     6361 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     6421 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     6481 cacctcgacc tgagacaatt gtccttttcc gctgcataac cctgcttcgg ggtcattata
     6541 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     6601 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     6661 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     6721 gggaatcctg ctctgcgagg ctggccgtag gccggcccta ccggcgcggc agcgttaccc
     6781 gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg ggcatcggca
     6841 ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc aggtctggtt
     6901 ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc ggtagttgct
     6961 gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac agcgattcgt
     7021 cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc aactggtcgc
     7081 ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg ccggggccgt
     7141 tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg tattggaccg
     7201 tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc acggcgttct
     7261 ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt ttcctcgcaa
     7321 taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga ccgggcttgt
     7381 tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc atgcggtagg
     7441 ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac
     7501 aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt tctttaacct acgcaatgag
     7561 ctattgcggg gggtgccgca atgagctgtt gcgtaccccc cttttttaag ttgttgattt
     7621 ttaagtcttt cgcatttcgc cctatatcta gttctttggt gcccaaagaa gggcacccct
     7681 gcggggttcc cccacgcctt cggcgcggct ccccctccgg caaaaagtgg cccctccggg
     7741 gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc cccttggaac
     7801 ccccgcactc gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc cttcgactgc
     7861 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg
     7921 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc
     7981 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg
     8041 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct
     8101 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac
     8161 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg
     8221 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggggg
     8281 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     8341 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
