LOCUS       Exported                3342 bp ds-DNA     circular SYN 19-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Doudna lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 10, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..54
                     /label=JW272
     primer_bind     complement(1..53)
                     /label=JW275
     CDS             24..680
                     /label=CmR
     primer_bind     24..42
                     /label=JW3
     primer_bind     362..385
                     /label=JW14
     terminator      701..795
                     /label=lambda t0 terminator
     misc_feature    798..819
                     /label=start cloning
     primer_bind     798..814
                     /label=JW222
     promoter        1038..1142
                     /label=AmpR promoter
     CDS             1143..1211
                     /label=AmpR
     CDS             1212..2003
                     /label=AmpR
     rep_origin      2174..2762
                     /label=ori
     primer_bind     2916..2934
                     /label=JW13
     primer_bind     complement(3032..3049)
                     /label=JW223
     misc_feature    3035..3049
                     /label=end cloning
     promoter        3075..3167
                     /label=cat promoter
     primer_bind     complement(3132..3187)
                     /label=JW273
     primer_bind     3137..3195
                     /label=JW274
     misc_feature    3149..3166
                     /label=end cloning pJW51
     misc_feature    join(3167..3342,1..23)
                     /label=47 array gblock
     misc_feature    join(3302..3342,1..23)
                     /label=cloning section
     misc_RNA        join(3330..3342,1..22)
                     /label=Repeats
                     /note="/created_by=User"
ORIGIN
        1 aaagccgaaa ggctgttaag acagagaaaa aaatcactgg atataccacc gttgatatat
       61 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      121 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      181 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      241 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      301 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      361 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      421 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      481 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      541 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      601 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
      661 agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt gatagatcca
      721 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
      781 tttattggtg agaatcctta agccagcccc gacacccgcc aacacccgct gacgcgccct
      841 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      901 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      961 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1381 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1681 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3001 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatc ctctagagga
     3061 tccaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     3121 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaaatc aacattaaaa
     3181 tatggtgcgg gaactatgaa aaacaaacaa aaacaaccat ccatacctca ccaaaatacc
     3241 cccggtaacc catcaaacac accccaaatc attctggtgc ggaaaatgca ttgcaactca
     3301 ttgaataagc agatagaatt tcaaccggag ttctagaaca cc
//
