LOCUS       pQE80X_Z17_Autot        5942 bp    DNA        circular     09-MAY-2022
DEFINITION  Carb resistance.
ACCESSION   urn.local...1r5-ca4h6xq
VERSION     urn.local...1r5-ca4h6xq
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            7..88
                     /standard_name="T5 Promoter"
                     /locus_tag="T5 Promoter"
                     /label="T5 Promoter"
                     /ApEinfo_label="T5 Promoter"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(86..114)
                     /standard_name="TMW1003"
                     /locus_tag="TMW1003"
                     /label="TMW1003"
                     /ApEinfo_label="TMW1003"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     gene            115..192
                     /standard_name="PelB"
                     /locus_tag="PelB"
                     /label="PelB"
                     /ApEinfo_label="PelB"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    163..192
                     /standard_name="5' flanking for CC synthesis"
                     /locus_tag="5' flanking for CC synthesis"
                     /label="5' flanking for CC synthesis"
                     /ApEinfo_label="5' flanking for CC synthesis"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     163..177
                     /standard_name="TMW535"
                     /locus_tag="TMW535"
                     /label="TMW535"
                     /ApEinfo_label="TMW535"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(174..192)
                     /standard_name="TMW541"
                     /locus_tag="TMW541"
                     /label="TMW541"
                     /ApEinfo_label="TMW541"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(316..333)
                     /standard_name="TMW658"
                     /locus_tag="TMW658"
                     /label="TMW658"
                     /ApEinfo_label="TMW658"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     316..330
                     /standard_name="TMW540"
                     /locus_tag="TMW540"
                     /label="TMW540"
                     /ApEinfo_label="TMW540"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(327..345)
                     /standard_name="TMW536"
                     /locus_tag="TMW536"
                     /label="TMW536"
                     /ApEinfo_label="TMW536"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     352..369
                     /standard_name="TMW657"
                     /locus_tag="TMW657"
                     /label="TMW657"
                     /ApEinfo_label="TMW657"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     gene            358..1377
                     /standard_name="Autotransporter"
                     /locus_tag="Autotransporter"
                     /label="Autotransporter"
                     /ApEinfo_label="Autotransporter"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1378..1399
                     /standard_name="TMW1002"
                     /locus_tag="TMW1002"
                     /label="TMW1002"
                     /ApEinfo_label="TMW1002"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     terminator      complement(1394..1499)
                     /locus_tag="terminator"
                     /label="terminator"
                     /ApEinfo_label="terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            1537..2196
                     /standard_name="camR"
                     /locus_tag="camR"
                     /label="camR"
                     /ApEinfo_label="camR"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      2261..2332
                     /locus_tag="terminator(1)"
                     /label="terminator(1)"
                     /ApEinfo_label="terminator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(2440..3522)
                     /gene="lacI"
                     /gene_synonym="ECK0342; JW0336"
                     /db_xref="ECOCYC:EG10525; EcoGene:EG10525; GeneID:945007"
                     /standard_name="lacI"
                     /locus_tag="lacI"
                     /label="lacI"
                     /ApEinfo_label="lacI"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      4033..4852
                     /locus_tag="rep_origin"
                     /label="rep_origin"
                     /ApEinfo_label="rep_origin"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(4877..5737)
                     /gene="AmpR"
                     /standard_name="AmpR"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    193..315
                     /locus_tag="Z18"
                     /label="Z18"
                     /ApEinfo_label="Z18"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gtcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaaa
      121 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      181 atgagaggat cgTCGATCGC TGCTACGTTA GAAAATGACT TAGCGCGTCT GGAAAATGAA
      241 AATGCTCGTC TGGAAAAGGA CATTGCGAAT CTGGAGCGTG ATTTGGCGAA ATTGGAGCGT
      301 GAGGAGGCTT ATTTCggtag cggtagcggc tctggatcag gcagcggatc actcgagaca
      361 cctacgccgg gtccggatct gaatgtggat aatgacctgc gaccggaggc gggtagctac
      421 attgcgaacc ttgcagcagc gaataccatg ttcaccacgc gtctgcatga gcgtctgggt
      481 aatacgtact ataccgacat ggtgacgggt gagcagaaac aaaccactat gtggatgcgc
      541 catgaaggtg gtcataataa atggcgtgat ggcagcggcc agctgaaaac ccaaagcaat
      601 cgctatgttc tgcaactggg aggcgatgtc gcgcagtgga gccaaaacgg cagcgaccgc
      661 tggcatgttg gggtcatggc gggatatggc aacagcgaca gcaaaaccat ttcctcgcga
      721 accggttatc gtgcaaaagc gagtgtgaac ggatatagca caggcctcta tgccacctgg
      781 tatgccgatg acgagtcgcg taatggcgcg tatctcgaca gttgggcgca gtacagctgg
      841 tttgataaca cagtgaaagg ggatgactta caaagtgaat cctataaatc aaaaggattt
      901 accgcttcac tggaagctgg atacaaacac aaattagctg aatttaatgg cagccaggga
      961 acgcgtaatg aatggtatgt tcagccgcaa gcacaggtta cctggatggg agtcaaagcc
     1021 gataagcacc gcgaaagcaa cggaaccctc gttcatagca acggtgatgg caatgttcaa
     1081 acccgacttg gcgtaaaaac ctggctgaag agccaccata aaatggatga cggtaaatcc
     1141 cgcgagttcc agccgtttgt agaagtgaac tggctacata acagtaagga tttcagcacc
     1201 agtatggatg gcgtgtctgt cactcaggat ggagcccgaa atattgctga gataaaaacc
     1261 ggggtggaag gacagctaaa tgccaacctg aatgtctggg ggaatgtggg cgttcaggtt
     1321 gccgataggg gatataatga cacctctgca atggttggca ttaagtggca attctaaaag
     1381 cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1441 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagct
     1501 agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1561 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1621 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1681 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1741 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1801 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1861 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1921 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1981 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2041 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2101 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2161 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     2221 ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac gaaaggctca
     2281 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     2341 gacaaatccg ccctctagat tacgtgcagt cgatgataag ctgtcaaaca tgagaattgt
     2401 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2461 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2521 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     2581 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     2641 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     2701 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     2761 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     2821 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     2881 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     2941 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     3001 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     3061 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     3121 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     3181 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     3241 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     3301 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     3361 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     3421 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     3481 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     3541 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc
     3601 ggaatttcgg gcagcgttgg gtcctggcca cgggtgcgca tgatctagag ctgcctcgcg
     3661 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3721 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3781 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     3841 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3901 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     3961 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4801 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4861 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4921 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4981 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5041 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5101 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5161 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5221 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5281 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5341 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5401 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5461 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5521 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5581 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5641 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5701 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5761 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5821 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5881 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     5941 ac     
//
