LOCUS       pQE80X_AP4_Autot        5918 bp    DNA        circular     09-MAY-2022
DEFINITION  Carb resistance.
ACCESSION   urn.local...1r5-ca4h6xq
VERSION     urn.local...1r5-ca4h6xq
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            7..88
                     /standard_name="T5 Promoter"
                     /locus_tag="T5 Promoter"
                     /label="T5 Promoter"
                     /ApEinfo_label="T5 Promoter"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(86..114)
                     /standard_name="TMW1003"
                     /locus_tag="TMW1003"
                     /label="TMW1003"
                     /ApEinfo_label="TMW1003"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     gene            115..192
                     /standard_name="PelB"
                     /locus_tag="PelB"
                     /label="PelB"
                     /ApEinfo_label="PelB"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    163..192
                     /standard_name="5' flanking for CC synthesis"
                     /locus_tag="5' flanking for CC synthesis"
                     /label="5' flanking for CC synthesis"
                     /ApEinfo_label="5' flanking for CC synthesis"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     163..177
                     /standard_name="TMW535"
                     /locus_tag="TMW535"
                     /label="TMW535"
                     /ApEinfo_label="TMW535"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(174..192)
                     /standard_name="TMW541"
                     /locus_tag="TMW541"
                     /label="TMW541"
                     /ApEinfo_label="TMW541"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(292..309)
                     /standard_name="TMW658"
                     /locus_tag="TMW658"
                     /label="TMW658"
                     /ApEinfo_label="TMW658"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     292..306
                     /standard_name="TMW540"
                     /locus_tag="TMW540"
                     /label="TMW540"
                     /ApEinfo_label="TMW540"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(303..321)
                     /standard_name="TMW536"
                     /locus_tag="TMW536"
                     /label="TMW536"
                     /ApEinfo_label="TMW536"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     328..345
                     /standard_name="TMW657"
                     /locus_tag="TMW657"
                     /label="TMW657"
                     /ApEinfo_label="TMW657"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     gene            334..1353
                     /standard_name="Autotransporter"
                     /locus_tag="Autotransporter"
                     /label="Autotransporter"
                     /ApEinfo_label="Autotransporter"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1354..1375
                     /standard_name="TMW1002"
                     /locus_tag="TMW1002"
                     /label="TMW1002"
                     /ApEinfo_label="TMW1002"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     terminator      complement(1370..1475)
                     /locus_tag="terminator"
                     /label="terminator"
                     /ApEinfo_label="terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            1513..2172
                     /standard_name="camR"
                     /locus_tag="camR"
                     /label="camR"
                     /ApEinfo_label="camR"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      2237..2308
                     /locus_tag="terminator(1)"
                     /label="terminator(1)"
                     /ApEinfo_label="terminator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(2416..3498)
                     /gene="lacI"
                     /gene_synonym="ECK0342; JW0336"
                     /db_xref="ECOCYC:EG10525; EcoGene:EG10525; GeneID:945007"
                     /standard_name="lacI"
                     /locus_tag="lacI"
                     /label="lacI"
                     /ApEinfo_label="lacI"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      4009..4828
                     /locus_tag="rep_origin"
                     /label="rep_origin"
                     /ApEinfo_label="rep_origin"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(4853..5713)
                     /gene="AmpR"
                     /standard_name="AmpR"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    193..291
                     /locus_tag="AP4"
                     /label="AP4"
                     /ApEinfo_label="AP4"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gtcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaaa
      121 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      181 atgagaggat cgTCTCCCGA GGATGAATTA GCAGCTAATG AAGAGGAACT GCAACAGAAC
      241 GAACAAAAGC TGGCTCAAAT TAAACAGAAG TTGCAGGCTA TCAAGTACGG Aggtagcggt
      301 agcggctctg gatcaggcag cggatcactc gagacaccta cgccgggtcc ggatctgaat
      361 gtggataatg acctgcgacc ggaggcgggt agctacattg cgaaccttgc agcagcgaat
      421 accatgttca ccacgcgtct gcatgagcgt ctgggtaata cgtactatac cgacatggtg
      481 acgggtgagc agaaacaaac cactatgtgg atgcgccatg aaggtggtca taataaatgg
      541 cgtgatggca gcggccagct gaaaacccaa agcaatcgct atgttctgca actgggaggc
      601 gatgtcgcgc agtggagcca aaacggcagc gaccgctggc atgttggggt catggcggga
      661 tatggcaaca gcgacagcaa aaccatttcc tcgcgaaccg gttatcgtgc aaaagcgagt
      721 gtgaacggat atagcacagg cctctatgcc acctggtatg ccgatgacga gtcgcgtaat
      781 ggcgcgtatc tcgacagttg ggcgcagtac agctggtttg ataacacagt gaaaggggat
      841 gacttacaaa gtgaatccta taaatcaaaa ggatttaccg cttcactgga agctggatac
      901 aaacacaaat tagctgaatt taatggcagc cagggaacgc gtaatgaatg gtatgttcag
      961 ccgcaagcac aggttacctg gatgggagtc aaagccgata agcaccgcga aagcaacgga
     1021 accctcgttc atagcaacgg tgatggcaat gttcaaaccc gacttggcgt aaaaacctgg
     1081 ctgaagagcc accataaaat ggatgacggt aaatcccgcg agttccagcc gtttgtagaa
     1141 gtgaactggc tacataacag taaggatttc agcaccagta tggatggcgt gtctgtcact
     1201 caggatggag cccgaaatat tgctgagata aaaaccgggg tggaaggaca gctaaatgcc
     1261 aacctgaatg tctgggggaa tgtgggcgtt caggttgccg ataggggata taatgacacc
     1321 tctgcaatgg ttggcattaa gtggcaattc taaaagctta attagctgag cttggactcc
     1381 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     1441 gccgccgggc gttttttatt ggtgagaatc caagctagct tggcgagatt ttcaggagct
     1501 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1561 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1621 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1681 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
     1741 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1801 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1861 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1921 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1981 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2041 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2101 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     2161 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ggtaatgact
     2221 ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2281 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccct ctagattacg
     2341 tgcagtcgat gataagctgt caaacatgag aattgtgcct aatgagtgag ctaacttaca
     2401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     2521 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     2581 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     2641 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
     2701 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     2761 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     2821 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     2881 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     2941 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     3001 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     3061 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     3121 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     3181 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     3241 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     3301 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     3361 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     3421 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     3481 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     3541 cataccgcga aaggttttgc accattcgat ggtgtcggaa tttcgggcag cgttgggtcc
     3601 tggccacggg tgcgcatgat ctagagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3661 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3721 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3781 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3841 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3901 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4501 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5881 ataaaaatag gcgtatcacg aggccctttc gtcttcac  
//
