LOCUS       Exported                6315 bp DNA     circular SYN 14-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSYn-DIO-Scramble-miR-IRES-mCitrine
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Chandra R, Francis TC, Konkalmatt P, Amgalan A, Gancarz AM, Dietz 
            DM, Lobo MK.
  TITLE     Opposing role for Egr3 in nucleus accumbens cell subtypes in cocaine
            action.
  JOURNAL   J Neurosci 2015 May 20;35:7927-37
  PUBMED    25995477
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Ramesh Chandra, Mary Kay Lobo
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2022 from SnapGene 6.0.4
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0548-15.2015"; journalName: "J Neurosci"; date: 
            "2015-05-20"; volume: "35"; pages: "7927-37"
COMMENT     Ligation of : AAV-DIO-hM3D(Gq)-IRES-mCitrine-direct [6136  nt] : 
            (StuI[5816] / NheI[4017]) to PCR(Egr3miR5D-NheI-EcoRV) [175  nt] : 
            (NheI[5] / EcoRV[182])
FEATURES             Location/Qualifiers
     source          1..6315
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=R-ITR
     promoter        156..640
                     /label=human Synapsin1 promoter
     protein_bind    671..704
                     /label=lox2272
                     /bound_moiety="Cre"
     protein_bind    755..788
                     /label=loxP
                     /bound_moiety="Cre"
     CDS             complement(800..1516)
                     /codon_start=1
                     /label=mCitrine
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      complement(1582..2073)
                     /label=IRES
                     /regulatory_class="ribosome_binding_site"
     3'UTR           complement(2140..2186)
                     /label=3'miR flanking region
     misc_feature    complement(2187..2250)
                     /label=miRScramble
     5'UTR           2251..2273
                     /label=5'miR flanking [*]
     protein_bind    complement(2300..2333)
                     /label=lox2272
                     /bound_moiety="Cre"
     protein_bind    complement(2384..2417)
                     /label=loxP
                     /bound_moiety="Cre"
     regulatory      2432..3040
                     /label=WPRE
                     /regulatory_class="other"
     polyA_signal    3060..3538
                     /label=hGH polyA
     misc_feature    3578..3718
                     /label=L-ITR
     misc_feature    3810..4110
                     /label=f1Origin
     misc_feature    4635..5492
                     /label=AmpR
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt
      661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
      781 gaagttatgg cgcgcctcac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      841 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagtttgg
      901 actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      961 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     1021 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     1081 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     1141 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     1201 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     1261 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcacatc aggccgtagc
     1321 cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     1381 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     1441 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     1501 cgcccttgct caccatggtg gcttatcatc gtgtttttca aaggaaaacc acgtccccgt
     1561 ggttcggggg gcctagacgt ttttttaacc tcgactaaac acatgtaaag catgtgcacc
     1621 gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt
     1681 tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc
     1741 actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc
     1801 acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc
     1861 ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt
     1921 tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg
     1981 ccctcacatt gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc
     2041 ggccttattc caagcggctt cggccagtaa cgttaggggg gggggaggga gaggggcgat
     2101 atcgcggccg cctacaagga tctcgagtgc ggccagatct gggccatttg ttccatgtga
     2161 gtgctagtaa caggccttgt gtcctgctga gttagctggt agtgcgtcag tcagtggcca
     2221 aaacgcacta ccagagctaa ctcagcagca tacagccttc agcaagcctc caggatccac
     2281 tggtcgactc actgctagca taacttcgta taaagtatcc tatacgaagt tatttgcctt
     2341 aacccagaaa ttatcactgt tattctttag aatggtgcaa agaataactt cgtataatgt
     2401 atgctatacg aagttatgaa ttcgatatca agcttatcga taatcaacct ctggattaca
     2461 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2521 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2581 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2641 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2701 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2761 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2821 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     2881 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2941 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3001 gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta
     3061 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     3121 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     3181 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     3241 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     3301 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     3361 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     3421 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     3481 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     3541 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     3601 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     3661 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     3721 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     3781 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     4021 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4081 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     4141 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4201 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     4261 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4321 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4381 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4441 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4501 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4561 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4621 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4681 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4741 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4801 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4861 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4921 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4981 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5041 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5101 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5161 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5221 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5281 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5341 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5401 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5461 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5521 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5581 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5641 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5701 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5761 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5821 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5881 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5941 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6001 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6061 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6241 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6301 cttttgctca catgt
//
