LOCUS       pDGB1alpha2_SulR        4404 bp    DNA     circular UNK 16-MAY-2022
DEFINITION  (GB0072,GB_UD_6A3B,GB0037)pDGB1_alpha2.
ACCESSION   GB_UA_4991
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        5..295
                     /vntifkey=29
                     /modified_by="kobercova"
                     /label="Pnos"
     misc_feature    185..194
                     /vntifkey=21
                     /label="CAAT\box"
     misc_feature    269..274
                     /vntifkey=21
                     /label="TATA"
     CDS             297..>1306
                     /modified_by="kobercova"
                     /label="sulI (CDS)"
     misc_feature    302
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     mutation        302
                     /created_by="kobercova"
                     /modified_by="kobercova"
                     /label="T->A"
     misc_feature    complement(1070..1085)
                     /Mismatches=0
                     /%GC="62.5"
                     /Hairpin_Tm="65.7"
                     /Self_Dimer_Tm="None"
                     /Tm="54.2"
                     /annotation_group="147273937479487aee363-9152-4951-87aa-96
                     ca22d6d40d"
                     /Sequence="CAATAGCGGAAGCCCC"
                     /created_by="primer3"
                     /label="SulR_PCR1_R  (binding)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1090..1105
                     /Mismatches=0
                     /%GC="62.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.1"
                     /Tm="55.5"
                     /annotation_group="1457013698120cd092336-cf6e-450c-b052-91
                     8a50e9a205"
                     /Sequence="CGGTGTCGCGGAAATC"
                     /created_by="primer3"
                     /label="SulR_PCR2_F (binding)"
     misc_feature    complement(1206..1221)
                     /Mismatches=0
                     /%GC="75.0"
                     /Hairpin_Tm="38.3"
                     /Self_Dimer_Tm="39.9"
                     /Tm="61.9"
                     /annotation_group="14728447091637acd7986-2694-4c26-b177-f1
                     f4c8b71a82"
                     /Sequence="CTCCAGGCGCGTGGGT"
                     /created_by="primer3"
                     /label="SulR_PCR2_R (binding)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1226..1241
                     /Mismatches=0
                     /%GC="62.5"
                     /Hairpin_Tm="52.7"
                     /Self_Dimer_Tm="11.5"
                     /Tm="56.6"
                     /annotation_group="14570765167014b1ad616-64dc-4e03-a19d-8b
                     80ba47cdc4"
                     /Sequence="GCGAAGCGCAATCACC"
                     /created_by="primer3"
                     /label="SulR_PCR3_F (binding)"
     modified_base   1307
                     /created_by="kobercova"
                     /label="Mismatch"
     misc_feature    1307
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     terminator      1308..1803
                     /vntifkey=43
                     /label="Tnos"
     mutation        1392
                     /vntifkey=62
                     /label="T>C"
     misc_feature    1804..1807
                     /vntifkey=21
                     /label="CGCT"
     repeat_region   1820..1844
                     /vntifkey=34
                     /label="RB"
     misc_feature    complement(1832..1852)
                     /Mismatches=0
                     /%GC="52.4"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="4.4"
                     /Tm="60.9"
                     /annotation_group="10509918323728f2967b3a-7e52-4b41-b656-5
                     cf905a1e2e6"
                     /Sequence="GCGACTTAGTTTACCCGCCAA"
                     /created_by="primer3"
                     /label="pDGB1_alpha R "
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(1870..2594)
                     /vntifkey=33
                     /label="pUC-ori"
     CDS             2690..3513
                     /vntifkey=4
                     /label="KanR"
     rep_origin      3795..4347
                     /vntifkey=33
                     /label="pSa"
     primer_bind     4318..4337
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="39.1"
                     /Self_Dimer_Tm="None"
                     /Tm="61.5"
                     /annotation_group="1050980787701045d0b557-3322-4b63-b1d4-7
                     6acfb45f9d4"
                     /Sequence="CAACCTCTCGGGCTTCTGGA"
                     /created_by="primer3"
                     /label="pDGB1_alpha F "
     misc_feature    4384..4387
                     /vntifkey=21
                     /label="TAAC"
     repeat_region   complement(4387..>4404)
                     /vntifkey=34
                     /label="LB"
     misc_feature    4401..4404
                     /vntifkey=21
                     /label="GTCA"
ORIGIN      
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcaatgg
      301 catctatgat ttcttcttca gctgtgacta cagtcagccg tgcttctacg gtgcaatcgg
      361 ccgctgtggc tccattcggc ggcctcaaat ccatgactgg attcccagtt aagaaggtca
      421 acactgacat tacttccatt acaagcaatg gtggaagagt aaagtgcatg gtgacggtgt
      481 tcggcattct gaatctcacc gaggactcct tcttcgatga gagccggcgg ctagaccccg
      541 ccggcgctgt cacggctgcg atcgaaatgc tgcgagtcgg atcagacgtc gtggatgtcg
      601 gaccggccgc cagccatccg gacgcgaggc cagtatcgcc ggccgatgag atcagacgta
      661 ttgcgccgct cttagacgcc ctgtccgatc agatgcaccg tgtttcaatc gacagcttcc
      721 aaccggaaac ccagcgctat gcgctcaagc gcggcgtggg ctacctgaac gacatccaag
      781 gatttcctga ccctgcgctc tatcccgata ttgctgaggc ggactgtagg ctggtggtta
      841 tgcactcagc gcagcgggat ggcatcgcca cccgcaccgg tcaccttcga cccgaagacg
      901 cgctcgacga gattgtgcgg ttcttcgagg cgcgggtttc cgccttgcga cggagcgggg
      961 tcgctgccga ccggctcatc ctcgatccgg ggatgggatt tttcttgagc cccgcaccgg
     1021 aaacatcgct gcacgtgctg tcgaaccttc aaaagctgaa gtcggcgttg gggcttccgc
     1081 tattggtatc ggtgtcgcgg aaatccttct tgggcgccac cgttggcctt cctgtaaagg
     1141 atctgggtcc agcgagcctt gcggcggaac ttcacgcgat cggcaatggc gctgactacg
     1201 tccgcaccca cgcgcctgga gatctgcgaa gcgcaatcac catctcggaa accctcgcga
     1261 aatttcgcag tcgcgacgcc agagatcgag ggttagatca tgcctaagct tggaatggat
     1321 cttcgatccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     1381 cggtcttgcg acgattatca tataatttct gttgaattac gttaagcatg taataattaa
     1441 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     1501 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcdc
     1561 ggtgtcatct atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc
     1621 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1681 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1741 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac
     1801 tcgcgcttga gacgaagctt gacaggatat attggcgggt aaactaagtc gctgtatgtg
     1861 tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1921 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1981 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2041 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2101 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2161 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2221 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2281 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2341 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2401 ctgaagccag ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc
     2461 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2521 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2581 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2641 aaatgaagtt ttaaatcaat ctaaagtata tatgtgtaac attggtctag tgattagaaa
     2701 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     2761 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     2821 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     2881 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     2941 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     3001 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     3061 gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     3121 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     3181 aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga
     3241 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     3301 accatctcat ctgtaacaac attggcaacg ctacctttgc catgtttcag aaacaactct
     3361 ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg
     3421 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag
     3481 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     3541 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     3601 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     3661 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     3721 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     3781 ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggcttt tttatccccg
     3841 gaagcctgtg gatagagggt agttatccac gtgaaaccgc taatgccccg caaagccttg
     3901 attcacgggg ctttccggcc cgctccaaaa actatccacg tgaaatcgct aatcagggta
     3961 cgtgaaatcg ctaatcggag tacgtgaaat cgctaataag gtcacgtgaa atcgctaatc
     4021 aaaaaggcac gtgagaacgc taatagccct ttcagatcaa cagcttgcaa acacccctcg
     4081 ctccggcaag tagttacagc aagtagtatg ttcaattagc ttttcaatta tgaatatata
     4141 tatcaattat tggtcgccct tggcttgtgg acaatgcgct acgcgcaccg gctccgcccg
     4201 tggacaaccg caagcggttg cccaccgtcg agcgcctttg cccacaaccc ggcggccggc
     4261 cgcaacagat cgttttataa attttttttt ttgaaaaaga aaaagcccga aaggcggcaa
     4321 cctctcgggc ttctggattt ccgatccccg gaattagaga tcttggcagg atatattgtg
     4381 gtgtaacaag cttcgtctca gtca
//
