LOCUS       Copy_of_Copy_of_pACYC-C 7593 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(535..1617)
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(1618..1695)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1741..1759
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1760..1784
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     RBS             1815..1820
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1828..1830
                     /label="ATG"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             1832..5734
                     /label="FnCp1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1832..5734
                     /label="Translation 1832-5734"
     misc_feature    1856..1857
                     /label="Del mut"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5780..5780
                     /label="MCS-2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      5804..5851
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(6071..6730)
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(6731..6833)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(join(7359..7593,1..310))
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ATGCACGAAC CCCCCGTTCA GTCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
       61 TCCAACCCGG AAAGACATGC AAAAGCACCA CTGGCAGCAG CCACTGGTAA TTGATTTAGA
      121 GGAGTTAGTC TTGAAGTCAT GCGCCGGTTA AGGCTAAACT GAAAGGACAA GTTTTGGTGA
      181 CTGCGCTCCT CCAAGCCAGT TACCTCGGTT CAAAGAGTTG GTAGCTCAGA GAACCTTCGA
      241 AAAACCGCCC TGCAAGGCGG TTTTTTCGTT TTCAGAGCAA GAGATTACGC GCAGACCAAA
      301 ACGATCTCAA GAAGATCATC TTATTAATCA GATAAAATAT TTCTAGATTT CAGTGCAATT
      361 TATCTCTTCA AATGTAGCAC CTGAAGTCAG CCCCATACGA TATAAGTTGT AATTCTCATG
      421 TTAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC
      481 GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG
      541 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
      601 GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG
      661 CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT
      721 GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA
      781 GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC GCAGCCCGGA
      841 CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT
      901 GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA
      961 GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC
     1021 AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG
     1081 GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT
     1141 AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA
     1201 GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT
     1261 GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC
     1321 TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC
     1381 AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG
     1441 TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC
     1501 TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC
     1561 GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT
     1621 CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG
     1681 CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAAT
     1741 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTGTA GAAATAATTT
     1801 TGTTTAACTT TAATAAGGAG ATATACCATG GATGTCAATT TATCAAGAAT TTGTTAATAA
     1861 ATATAGTTTA AGTAAAACTC TAAGATTTGA GTTAATCCCA CAGGGTAAAA CACTTGAAAA
     1921 CATAAAAGCA AGAGGTTTGA TTTTAGATGA TGAGAAAAGA GCTAAAGACT ACAAAAAGGC
     1981 TAAACAAATA ATTGATAAAT ATCATCAGTT TTTTATAGAG GAGATATTAA GTTCGGTTTG
     2041 TATTAGCGAA GATTTATTAC AAAACTATTC TGATGTTTAT TTTAAACTTA AAAAGAGTGA
     2101 TGATGATAAT CTACAAAAAG ATTTTAAAAG TGCAAAAGAT ACGATAAAGA AACAAATATC
     2161 TGAATATATA AAGGACTCAG AGAAATTTAA GAATTTGTTT AATCAAAACC TTATCGATGC
     2221 TAAAAAAGGG CAAGAGTCAG ATTTAATTCT ATGGCTAAAG CAATCTAAGG ATAATGGTAT
     2281 AGAACTATTT AAAGCCAATA GTGATATCAC AGATATAGAT GAGGCGTTAG AAATAATCAA
     2341 ATCTTTTAAA GGTTGGACAA CTTATTTTAA GGGTTTTCAT GAAAATAGAA AAAATGTTTA
     2401 TAGTAGCAAT GATATTCCTA CATCTATTAT TTATAGGATA GTAGATGATA ATTTGCCTAA
     2461 ATTTCTAGAA AATAAAGCTA AGTATGAGAG TTTAAAAGAC AAAGCTCCAG AAGCTATAAA
     2521 CTATGAACAA ATTAAAAAAG ATTTGGCAGA AGAGCTAACC TTTGATATTG ACTACAAAAC
     2581 ATCTGAAGTT AATCAAAGAG TTTTTTCACT TGATGAAGTT TTTGAGATAG CAAACTTTAA
     2641 TAATTATCTA AATCAAAGTG GTATTACTAA ATTTAATACT ATTATTGGTG GTAAATTTGT
     2701 AAATGGTGAA AATACAAAGA GAAAAGGTAT AAATGAATAT ATAAATCTAT ACTCACAGCA
     2761 AATAAATGAT AAAACACTCA AAAAATATAA AATGAGTGTT TTATTTAAGC AAATTTTAAG
     2821 TGATACAGAA TCTAAATCTT TTGTAATTGA TAAGTTAGAA GATGATAGTG ATGTAGTTAC
     2881 AACGATGCAA AGTTTTTATG AGCAAATAGC AGCTTTTAAA ACAGTAGAAG AAAAATCTAT
     2941 TAAAGAAACA CTATCTTTAT TATTTGATGA TTTAAAAGCT CAAAAACTTG ATTTGAGTAA
     3001 AATTTATTTT AAAAATGATA AATCTCTTAC TGATCTATCA CAACAAGTTT TTGATGATTA
     3061 TAGTGTTATT GGTACAGCGG TACTAGAATA TATAACTCAA CAAATAGCAC CTAAAAATCT
     3121 TGATAACCCT AGTAAGAAAG AGCAAGAATT AATAGCCAAA AAAACTGAAA AAGCAAAATA
     3181 CTTATCTCTA GAAACTATAA AGCTTGCCTT AGAAGAATTT AATAAGCATA GAGATATAGA
     3241 TAAACAGTGT AGGTTTGAAG AAATACTTGC AAACTTTGCG GCTATTCCGA TGATATTTGA
     3301 TGAAATAGCT CAAAACAAAG ACAATTTGGC ACAGATATCT ATCAAATATC AAAATCAAGG
     3361 TAAAAAAGAC CTACTTCAAG CTAGTGCGGA AGATGATGTT AAAGCTATCA AGGATCTTTT
     3421 AGATCAAACT AATAATCTCT TACATAAACT AAAAATATTT CATATTAGTC AGTCAGAAGA
     3481 TAAGGCAAAT ATTTTAGACA AGGATGAGCA TTTTTATCTA GTATTTGAGG AGTGCTACTT
     3541 TGAGCTAGCG AATATAGTGC CTCTTTATAA CAAAATTAGA AACTATATAA CTCAAAAGCC
     3601 ATATAGTGAT GAGAAATTTA AGCTCAATTT TGAGAACTCG ACTTTGGCTA ATGGTTGGGA
     3661 TAAAAATAAA GAGCCTGACA ATACGGCAAT TTTATTTATC AAAGATGATA AATATTATCT
     3721 GGGTGTGATG AATAAGAAAA ATAACAAAAT ATTTGATGAT AAAGCTATCA AAGAAAATAA
     3781 AGGCGAGGGT TATAAAAAAA TTGTTTATAA ACTTTTACCT GGCGCAAATA AAATGTTACC
     3841 TAAGGTTTTC TTTTCTGCTA AATCTATAAA ATTTTATAAT CCTAGTGAAG ATATACTTAG
     3901 AATAAGAAAT CATTCCACAC ATACAAAAAA TGGTAGTCCT CAAAAAGGAT ATGAAAAATT
     3961 TGAGTTTAAT ATTGAAGATT GCCGAAAATT TATAGATTTT TATAAACAGT CTATAAGTAA
     4021 GCATCCGGAG TGGAAAGATT TTGGATTTAG ATTTTCTGAT ACTCAAAGAT ATAATTCTAT
     4081 AGATGAATTT TATAGAGAAG TTGAAAATCA AGGCTACAAA CTAACTTTTG AAAATATATC
     4141 AGAGAGCTAT ATTGATAGCG TAGTTAATCA GGGTAAATTG TACCTATTCC AAATCTATAA
     4201 TAAAGATTTT TCAGCTTATA GCAAAGGGCG ACCAAATCTA CATACTTTAT ATTGGAAAGC
     4261 GCTGTTTGAT GAGAGAAATC TTCAAGATGT GGTTTATAAG CTAAATGGTG AGGCAGAGCT
     4321 TTTTTATCGT AAACAATCAA TACCTAAAAA AATCACTCAC CCAGCTAAAG AGGCAATAGC
     4381 TAATAAAAAC AAAGATAATC CTAAAAAAGA GAGTGTTTTT GAATATGATT TAATCAAAGA
     4441 TAAACGCTTT ACTGAAGATA AGTTTTTCTT TCACTGTCCT ATTACAATCA ATTTTAAATC
     4501 TAGTGGAGCT AATAAGTTTA ATGATGAAAT CAATTTATTG CTAAAAGAAA AAGCAAATGA
     4561 TGTTCATATA TTAAGTATAG ATAGAGGTGA AAGACATTTA GCTTACTATA CTTTGGTAGA
     4621 TGGTAAAGGC AATATCATCA AACAAGATAC TTTCAACATC ATTGGTAATG ATAGAATGAA
     4681 AACAAACTAC CATGATAAGC TTGCTGCAAT AGAGAAAGAT AGGGATTCAG CTAGGAAAGA
     4741 CTGGAAAAAG ATAAATAACA TCAAAGAGAT GAAAGAGGGC TATCTATCTC AGGTAGTTCA
     4801 TGAAATAGCT AAGCTAGTTA TAGAGTATAA TGCTATTGTG GTTTTTGAGG ATTTAAATTT
     4861 TGGATTTAAA AGAGGGCGTT TCAAGGTAGA GAAGCAGGTC TATCAAAAGT TAGAAAAAAT
     4921 GCTAATTGAG AAACTAAACT ATCTAGTTTT CAAAGATAAT GAGTTTGATA AAACTGGGGG
     4981 AGTGCTTAGA GCTTATCAGC TAACAGCACC TTTTGAGACT TTTAAAAAGA TGGGTAAACA
     5041 AACAGGTATT ATCTACTATG TACCAGCTGG TTTTACTTCA AAAATTTGTC CTGTAACTGG
     5101 TTTTGTAAAT CAGTTATATC CTAAGTATGA AAGTGTCAGC AAATCTCAAG AGTTCTTTAG
     5161 TAAGTTTGAC AAGATTTGTT ATAACCTTGA TAAGGGCTAT TTTGAGTTTA GTTTTGATTA
     5221 TAAAAACTTT GGTGACAAGG CTGCCAAAGG CAAGTGGACT ATAGCTAGCT TTGGGAGTAG
     5281 ATTGATTAAC TTTAGAAATT CAGATAAAAA TCATAATTGG GATACTCGAG AAGTTTATCC
     5341 AACTAAAGAG TTGGAGAAAT TGCTAAAAGA TTATTCTATC GAATATGGGC ATGGCGAATG
     5401 TATCAAAGCA GCTATTTGCG GTGAGAGCGA CAAAAAGTTT TTTGCTAAGC TAACTAGTGT
     5461 CCTAAATACT ATCTTACAAA TGCGTAACTC AAAAACAGGT ACTGAGTTAG ATTATCTAAT
     5521 TTCACCAGTA GCAGATGTAA ATGGCAATTT CTTTGATTCG CGACAGGCGC CAAAAAATAT
     5581 GCCTCAAGAT GCTGATGCCA ATGGTGCTTA TCATATTGGG CTAAAAGGTC TGATGCTACT
     5641 AGGTAGGATC AAAAATAATC AAGAGGGCAA AAAACTCAAT TTGGTTATCA AAAATGAAGA
     5701 GTATTTTGAG TTCGTGCAGA ATAGGAATAA CTAacccagA GAATTCGAGC TCGGCGCGCC
     5761 TGCAGGTCGA CAAGCTTGCG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
     5821 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAACCT CAGGCATTTG AGAAGCACAC
     5881 GGTCACACTG CTTCCGGTAG TCAATAAACC GGTAAACCAG CAATAGACAT AAGCGGCTAT
     5941 TTAACGACCC TGCCCTGAAC CGACGACCGG GTCGAATTTG CTTTCGAATT TCTGCCATTC
     6001 ATCCGCTTAT TATCACTTAT TCAGGCGTAG CACCAGGCGT TTAAGGGCAC CAATAACTGC
     6061 CTTAAAAAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA
     6121 TTCTGCCGAC ATGGAAGCCA TCACAGACGG CATGATGAAC CTGAATCGCC AGCGGCATCA
     6181 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATAGTGAA AACGGGGGCG AAGAAGTTGT
     6241 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA
     6301 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA
     6361 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG
     6421 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA
     6481 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG
     6541 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA
     6601 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG
     6661 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT
     6721 TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG
     6781 GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC
     6841 ATTTTCGCCA AAAGTTGGCC CAGGGCTTCC CGGTATCAAC AGGGACACCA GGATTTATTT
     6901 ATTCTGCGAA GTGATCTTCC GTCACAGGTA TTTATTCGGC GCAAAGTGCG TCGGGTGATG
     6961 CTGCCAACTT ACTGATTTAG TGTATGATGG TGTTTTTGAG GTGCTCCAGT GGCTTCTGTT
     7021 TCTATCAGCT GTCCCTCCTG TTCAGCTACT GACGGGGTGG TGCGTAACGG CAAAAGCACC
     7081 GCCGGACATC AGCGCTAGCG GAGTGTATAC TGGCTTACTA TGTTGGCACT GATGAGGGTG
     7141 TCAGTGAAGT GCTTCATGTG GCAGGAGAAA AAAGGCTGCA CCGGTGCGTC AGCAGAATAT
     7201 GTGATACAGG ATATATTCCG CTTCCTCGCT CACTGACTCG CTACGCTCGG TCGTTCGACT
     7261 GCGGCGAGCG GAAATGGCTT ACGAACGGGG CGGAGATTTC CTGGAAGATG CCAGGAAGAT
     7321 ACTTAACAGG GAAGTGAGAG GGCCGCGGCA AAGCCGTTTT TCCATAGGCT CCGCCCCCCT
     7381 GACAAGCATC ACGAAATCTG ACGCTCAAAT CAGTGGTGGC GAAACCCGAC AGGACTATAA
     7441 AGATACCAGG CGTTTCCCCT GGCGGCTCCC TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT
     7501 TTACCGGTGT CATTCCGCTG TTATGGCCGC GTTTGTCTCA TTCCACGCCT GACACTCAGT
     7561 TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC TGT
//