LOCUS       Copy_of_Copy_of_pACYCDu 7413 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
KEYWORDS    "creator:Novagen (EMD Millipore)" "marker:CmR"                     
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     CDS             complement(535..1617)
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(1618..1695)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1741..1759
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1760..1784
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     RBS             1815..1820
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1828..1830
                     /label="ATG"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             1840..1857
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1871..1876
                     /label="RBS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1884..5273
                     /label="C2c1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1884..5273
                     /label="Translation 1884-5273"
     CDS             1884..5273
                     /label="Translation 1884-5273"
     promoter        5376..5394
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    5395..5419
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     RBS             5448..5453
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5459..5600
                     /label="MCS-2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             5462..5464
                     /label="ATG"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             5528..5572
                     /label="S-Tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      5624..5671
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5891..6550)
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(6551..6653)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(join(7179..7413,1..310))
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ATGCACGAAC CCCCCGTTCA GTCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
       61 TCCAACCCGG AAAGACATGC AAAAGCACCA CTGGCAGCAG CCACTGGTAA TTGATTTAGA
      121 GGAGTTAGTC TTGAAGTCAT GCGCCGGTTA AGGCTAAACT GAAAGGACAA GTTTTGGTGA
      181 CTGCGCTCCT CCAAGCCAGT TACCTCGGTT CAAAGAGTTG GTAGCTCAGA GAACCTTCGA
      241 AAAACCGCCC TGCAAGGCGG TTTTTTCGTT TTCAGAGCAA GAGATTACGC GCAGACCAAA
      301 ACGATCTCAA GAAGATCATC TTATTAATCA GATAAAATAT TTCTAGATTT CAGTGCAATT
      361 TATCTCTTCA AATGTAGCAC CTGAAGTCAG CCCCATACGA TATAAGTTGT AATTCTCATG
      421 TTAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC
      481 GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG
      541 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
      601 GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG
      661 CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT
      721 GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA
      781 GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC GCAGCCCGGA
      841 CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT
      901 GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA
      961 GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC
     1021 AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG
     1081 GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT
     1141 AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA
     1201 GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT
     1261 GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC
     1321 TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC
     1381 AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG
     1441 TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC
     1501 TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC
     1561 GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT
     1621 CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG
     1681 CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAAT
     1741 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTGTA GAAATAATTT
     1801 TGTTTAACTT TAATAAGGAG ATATACCATG GGCAGCAGCC ATCACCATCA TCACCACAGC
     1861 CAGGATCCTT AGGAGGGCGC TAGatggccg tcaaatccat caaagtgaaa cttcgtctcg
     1921 acgatatgcc ggagattcgg gccggtctat ggaaacttca taaggaagtc aatgcggggg
     1981 ttcgatatta cacggaatgg ctcagtcttc tccgtcaaga gaacttgtat cgaagaagtc
     2041 cgaatgggga cggagagcaa gaatgtgata agactgcaga agaatgcaaa gccgaattgt
     2101 tggagcggct gcgcgcgcgt caagtggaga atggacaccg tggtccggcg ggatcggacg
     2161 atgaattgct gcagttggcg cgtcaactct atgagttgtt ggttccgcag gcgataggtg
     2221 cgaaaggcga cgcgcagcaa attgcccgca aatttttgag ccccttggcc gacaaggacg
     2281 cagttggtgg gcttggaatc gcgaaggcgg ggaacaaacc gcggtgggtt cgcatgcgcg
     2341 aagcggggga accaggctgg gaagaggaga aggagaaggc tgagacgagg aaatctgcgg
     2401 atcggactgc ggatgttttg cgcgcgctcg cggattttgg gttaaagcca ctgatgcgcg
     2461 tatacaccga ttctgagatg tcatcggtgg agtggaaacc gcttcggaag ggacaagccg
     2521 ttcggacgtg ggatagggac atgttccaac aagctatcga acggatgatg tcgtgggagt
     2581 cgtggaatca gcgcgttggg caagagtacg cgaaactcgt agaacaaaaa aatcgatttg
     2641 agcagaagaa tttcgtcggc caggaacatc tggtccatct cgtcaatcag ttgcaacaag
     2701 atatgaaaga agcatcgccc ggactcgaat cgaaagagca aaccgcgcac tatgtgacgg
     2761 gacgggcatt gcgcggatcg gacaaggtat ttgagaagtg ggggaaactc gcccccgatg
     2821 cacctttcga tttgtacgac gccgaaatca agaatgtgca gagacgtaac acgagacgat
     2881 tcggatcaca tgacttgttc gcaaaattgg cagagccaga gtatcaggcc ctgtggcgcg
     2941 aagatgcttc gtttctcacg cgttacgcgg tgtacaacag catccttcgc aaactgaatc
     3001 acgccaaaat gttcgcgacg tttactttgc cggatgcaac ggcgcacccg atttggactc
     3061 gcttcgataa attgggtggg aatttgcacc agtacacctt tttgttcaac gaatttggag
     3121 aacgcaggca cgcgattcgt tttcacaagc tattgaaagt cgagaatggt gtcgcaagag
     3181 aagttgatga tgtcaccgtg cccatttcaa tgtcagagca attggataat ctgcttccca
     3241 gagatcccaa tgaaccgatt gcgctatatt ttcgagatta cggagccgaa cagcatttca
     3301 caggtgaatt tggtggcgcg aagatccagt gccgccggga tcagctggct catatgcacc
     3361 gacgcagagg ggcgagggat gtttatctca atgtcagcgt acgtgtgcag agtcagtctg
     3421 aggcgcgggg agaacgtcgc ccgccgtatg cggcagtatt tcgtctggtc ggggacaacc
     3481 atcgcgcgtt tgtccatttc gataaactat cggattatct tgcggaacat ccggatgatg
     3541 ggaagctcgg gtcggagggg ttgctttccg ggctgcgggt gatgagtgtc gatctcggcc
     3601 ttcgcacatc tgcatcgatt tccgtttttc gcgttgcccg gaaggacgag ttgaagccga
     3661 actcaaaagg tcgtgtaccg tttttctttc cgataaaagg gaatgacaat ctcgtcgcgg
     3721 ttcatgagcg atcacaactc ttgaagctgc ctggcgaaac ggagtcgaag gacctgcgtg
     3781 ctatccgaga agaacgccaa cggacattgc ggcagttgcg gacgcaactg gcgtatttgc
     3841 ggctgctcgt gcggtgtggg tcggaagatg tggggcggcg tgaacggagt tgggcaaagc
     3901 ttatcgagca gccggtggat gcggccaatc acatgacacc ggattggcgc gaggcttttg
     3961 aaaacgaact tcagaagctt aagtcactcc atggtatctg tagcgacaag gaatggatgg
     4021 atgctgtcta cgagagcgtt cgccgcgtgt ggcgtcacat gggcaaacag gttcgcgatt
     4081 ggcgaaagga cgtacgaagc ggagagcggc ccaagattcg cggctatgcg aaagacgtgg
     4141 tcggtggaaa ctcgattgag caaatcgagt atctggaacg tcagtacaag ttcctcaaga
     4201 gttggagctt ctttggtaag gtgtcgggac aagtgattcg tgcggagaag ggatctcgtt
     4261 ttgcgatcac gctgcgcgaa cacattgatc acgcgaagga agatcggctg aagaaattgg
     4321 cggatcgcat cattatggag gctctcggct atgtgtacgc gttggatgag cgcggcaaag
     4381 gaaagtgggt tgcgaagtat ccgccgtgcc agctcatcct gctggaggaa ttgagcgagt
     4441 accagttcaa taacgacagg cctccgagcg aaaacaacca gttgatgcaa tggagtcatc
     4501 gcggcgtgtt ccaggagttg ataaatcagg cccaagtcca tgatttactc gttgggacga
     4561 tgtatgcagc gttctcgtcg cgattcgacg cgcgaactgg ggcaccgggt atccgctgtc
     4621 gccgggttcc ggcgcgttgc acccaggagc acaatccaga accatttcct tggtggctga
     4681 acaagtttgt ggtggaacat acgttggatg cttgtcccct acgcgcagac gacctcatcc
     4741 caacgggtga aggagagatt tttgtctcgc cgttcagcgc ggaggagggg gactttcatc
     4801 agattcacgc cgacctgaat gcggcgcaaa atctgcagca gcgactctgg tctgattttg
     4861 atatcagtca aattcggttg cggtgtgatt ggggtgaagt ggacggtgaa ctcgttctga
     4921 tcccaaggct tacaggaaaa cgaacggcgg attcatatag caacaaggtg ttttatacca
     4981 atacaggtgt cacctattat gagcgagagc gggggaagaa gcggagaaag gttttcgcgc
     5041 aagagaaatt gtcggaggaa gaggcggagt tgctcgtgga agcagacgag gcgagggaga
     5101 aatcggtcgt tttgatgcgt gatccgtctg gcatcatcaa tcggggaaat tggaccaggc
     5161 aaaaggaatt ttggtcgatg gtgaaccagc ggatcgaagg atacttggtc aagcagattc
     5221 gctcgcgcgt tccattacaa gatagtgcgt gtgaaaacac gggggatatt taaGAATTCG
     5281 AGCTCGGCGC GCCTGCAGGT CGACAAGCTT GCGGCCGCAT AATGCTTAAG TCGAACAGAA
     5341 AGTAATCGTA TTGTACACGG CCGCATAATC GAAATTAATA CGACTCACTA TAGGGGAATT
     5401 GTGAGCGGAT AACAATTCCC CATCTTAGTA TATTAGTTAA GTATAAGAAG GAGATATACA
     5461 TATGGCAGAT CTCAATTGGA TATCGGCCGG CCACGCGATC GCTGACGTCG GTACCCTCGA
     5521 GTCTGGTAAA GAAACCGCTG CTGCGAAATT TGAACGCCAG CACATGGACT CGTCTACTAG
     5581 CGCAGCTTAA TTAACCTAGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
     5641 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAACCT CAGGCATTTG AGAAGCACAC
     5701 GGTCACACTG CTTCCGGTAG TCAATAAACC GGTAAACCAG CAATAGACAT AAGCGGCTAT
     5761 TTAACGACCC TGCCCTGAAC CGACGACCGG GTCGAATTTG CTTTCGAATT TCTGCCATTC
     5821 ATCCGCTTAT TATCACTTAT TCAGGCGTAG CACCAGGCGT TTAAGGGCAC CAATAACTGC
     5881 CTTAAAAAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA
     5941 TTCTGCCGAC ATGGAAGCCA TCACAGACGG CATGATGAAC CTGAATCGCC AGCGGCATCA
     6001 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATAGTGAA AACGGGGGCG AAGAAGTTGT
     6061 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA
     6121 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA
     6181 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG
     6241 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA
     6301 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG
     6361 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA
     6421 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG
     6481 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT
     6541 TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG
     6601 GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC
     6661 ATTTTCGCCA AAAGTTGGCC CAGGGCTTCC CGGTATCAAC AGGGACACCA GGATTTATTT
     6721 ATTCTGCGAA GTGATCTTCC GTCACAGGTA TTTATTCGGC GCAAAGTGCG TCGGGTGATG
     6781 CTGCCAACTT ACTGATTTAG TGTATGATGG TGTTTTTGAG GTGCTCCAGT GGCTTCTGTT
     6841 TCTATCAGCT GTCCCTCCTG TTCAGCTACT GACGGGGTGG TGCGTAACGG CAAAAGCACC
     6901 GCCGGACATC AGCGCTAGCG GAGTGTATAC TGGCTTACTA TGTTGGCACT GATGAGGGTG
     6961 TCAGTGAAGT GCTTCATGTG GCAGGAGAAA AAAGGCTGCA CCGGTGCGTC AGCAGAATAT
     7021 GTGATACAGG ATATATTCCG CTTCCTCGCT CACTGACTCG CTACGCTCGG TCGTTCGACT
     7081 GCGGCGAGCG GAAATGGCTT ACGAACGGGG CGGAGATTTC CTGGAAGATG CCAGGAAGAT
     7141 ACTTAACAGG GAAGTGAGAG GGCCGCGGCA AAGCCGTTTT TCCATAGGCT CCGCCCCCCT
     7201 GACAAGCATC ACGAAATCTG ACGCTCAAAT CAGTGGTGGC GAAACCCGAC AGGACTATAA
     7261 AGATACCAGG CGTTTCCCCT GGCGGCTCCC TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT
     7321 TTACCGGTGT CATTCCGCTG TTATGGCCGC GTTTGTCTCA TTCCACGCCT GACACTCAGT
     7381 TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC TGT
//