LOCUS       pDYT001:_pPCT60_piggyBA 9159 bp ds-DNA     circular     10-MAY-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    812..831
                     /label="BII(R)"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    874..936
                     /label="5'ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    887..917
                     /label="31bp spacer"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_fwdcolor="#0080c0"
     misc_feature    1357..1369
                     /label="EF1 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1370..1396
                     /label="MC_gbpr65"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     chromatin       1393..1633
                     /label="minUCOE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1407..1415)
                     /label="CTCF binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1582..1595
                     /label="CTCF binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2005..2013
                     /label="missing in actual plasmid"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2072..2073
                     /label="origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2074..2152
                     /label="origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        2074..2152
                     /label="EF-1ÃÂÃÂÃÂÃÂ± promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2074..2152
                     /label="EFS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2153..3251
                     /label="hEF1alpha promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3252..3271
                     /label="MC_gbpr64"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3383..3549
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3401..3401
                     /label="gbPrimerEnd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3551..4093
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4098..4105
                     /label="Pac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4132..4145
                     /label="intBC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4206..4206
                     /label="G-clamp"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    4206..4229
                     /label="site1:Bao_ADE2.d"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4227..4229
                     /label="PAM"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    4260..4260
                     /label="G-clamp"
                     /ApEinfo_revcolor="#804000"
                     /ApEinfo_fwdcolor="#804000"
     misc_feature    4260..4260
                     /label="Bri1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4261..4283
                     /label="site2:Bri1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4281..4283
                     /label="PAM"
                     /ApEinfo_revcolor="#804000"
                     /ApEinfo_fwdcolor="#804000"
     misc_feature    4314..4314
                     /label="G-clamp"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    4314..4337
                     /label="site3:whiteB"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_fwdcolor="#cc66ff"
     misc_feature    4335..4337
                     /label="PAM"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    4391..4396
                     /label="HpaI"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_fwdcolor="#808080"
     misc_feature    4398..4419
                     /label="10x capture site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4421..4551
                     /label="SV40 polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4794..4798
                     /label="XbaI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4799..5077
                     /label="bU6-2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4869..4886
                     /label="SPH"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4887..4894
                     /label="OCT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5023..5042
                     /label="PSE"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5058..5065
                     /label="TATA box"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5078..5088
                     /label="BstXI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5078..5088
                     /label="bU6-2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5089..5109
                     /label="whtB (perfect)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5093..5093
                     /label="mis-match III"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5110..5195
                     /label="sgRNA F + E constant region"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5114..5114
                     /label="A-U flip"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5118..5124
                     /label="BlpI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5145..5145
                     /label="A-U flip"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     PCR_primer      complement(5180..5195)
                     /label="BA100"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5196..5202
                     /label="polyT-track"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    complement(5203..5222)
                     /label="triple-assembly-1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     PCR_primer      complement(5203..5222)
                     /label="BA278"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(5223..5536)
                     /label="ANTIPARALLEL - mouse U6 - modified"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5417..5420
                     /label="loxP"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5537..5556
                     /label="site1:Bao_ADE2.d"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5547..5547
                     /label="mis-match II"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5557..5562
                     /label="lower stem part 1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5557..5571
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5561..5561
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5565..5571
                     /label="BlpI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5565..5571
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5572..5579
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5572..5579
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5572..5579
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5580..5586
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5580..5612
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5591..5596
                     /label="lower stem part 2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5592..5592
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5597..5612
                     /label="Nexus"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5610..5612
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5613..5651
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5631..5631
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5641..5641
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5652..5670
                     /label="BA281"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(5652..5670)
                     /label="BA280"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(5652..5670)
                     /label="triple-assembly-2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        complement(5671..5946)
                     /label="hU6 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5937..5947)
                     /label="BstXI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5948..5968
                     /label="bri1 (perfect)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5953..5953
                     /label="mis-match III"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5969..5974
                     /label="lower stem part 1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5969..5983
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5973..5973
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5977..5983
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5977..5983
                     /label="BlpI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    5984..5991
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5984..5991
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5984..5991
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5992..5998
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5992..6024
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6003..6008
                     /label="lower stem part 2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6004..6004
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6009..6021
                     /label="Nexus"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    6022..6024
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6025..6063
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6043..6043
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6053..6053
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(6064..6069)
                     /label="XhoI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    6075..6080
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    6086..6092
                     /label="NotI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    6613..6647
                     /label="3'ITR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     primer_bind     complement(7166..7185)
                     /label="pLac(F)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(8302..9159)
                     /label="Amp"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC ACGGCCAAGG ATCTGCGATa tcgataagct
     1381 tgatatcgaa ttcgggaggt ggtccctgca gttacgccaa tgataacccc cgccagaaaa
     1441 atcttagtag ccttcccttt ttgttttccg tgccccaact cggcggattg actcggcccc
     1501 ttccggaaac acccgaatca acttctagtc aaattattgt tcacgccgca atgacccacc
     1561 cctggcccgc gtctgtggaa ctgacccctg gtgtacagga gagttcgctg ctgaaagtgg
     1621 tcccaaaggg gtactagttt ttaagctccc aactccccct cccccagcgt ctggaggatt
     1681 ccacaccctc gcaccgcagg ggcgaggaag tgggcggagt ccggttttgg cgccagccgc
     1741 tgaggctgcc aagcagaaaa gccaccgctg aggagactcc ggtcactgtc ctcgccccgc
     1801 ctcccccttc cctccccttg gggaccaccg ggcgccacgc cgcgaacggt aagtgccgcg
     1861 gtcgtcggcg cctccgccct ccccctaggg ccccaattcc cagcgggcgc ggcgcgcggc
     1921 ccctcccccc gccgggcgcg cgcccgctgc cccgcccttc gtggccgccc ggcgtgggcg
     1981 gtgccacccc tccccccggc ggcccccctg cagccgcgcg cagctcccgg ctccctcccc
     2041 cttcggatgt ggcttgagct gtaggcgcgg aggggctccg gtgcccgtca gtgggcagag
     2101 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2161 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2221 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2281 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     2341 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     2401 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2461 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     2521 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2581 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     2641 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2701 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2761 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2821 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2881 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     2941 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3001 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3061 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3121 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3181 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3241 caggtgtcgt ggaattctca cgcgtctgca gCTAGCTATG GTGAGCAAGG GCGACACTGC
     3301 TCGATCCGCT CGCACCTGAC TGCTACGTGA CTACGCTGAT GTACAGATCC ATACTGCATG
     3361 TTCATTGACG ATCTACCTAG CTatggtgag caagggcgag gaggataaca tggccatcat
     3421 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     3481 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3541 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     3601 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3661 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     3721 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     3781 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     3841 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     3901 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3961 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     4021 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     4081 gctgtacaag taaTGAATTA ATtaaGTCAC GAATCCAGCT AGCTGTGCAG CNNNNNNNNN
     4141 NNNNNATTCA ACTGCAGTAA TGCTACCTCG TACTCACGCT TTCCAAGTGC TTGGCGTCGC
     4201 ATCTCGGTCC TTTGTACGCC GAAAAATGGC CTGACAACTA AGCTACGGCA CGCTGCCATG
     4261 TTGGGTCATA ACGATATCTC TGGTTCATCC GTGACCGAAC ATGTCATGGA GTAGCAGGAG
     4321 CTATTAATTC GCGGAGGACA ATGCGGTTCG TAGTCACTGT CTTCCGCAAT CGTCCATCGC
     4381 TCCTGCAGGT GTTAACTGCT CACCTATTAG CGGCTAAGGA AACTTGTTTA TTGCAGCTTA
     4441 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
     4501 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGAATTGACT
     4561 CAAATGATGT CAATTAGTCT ATCAGAAGCT CATCTGGTCT CCCTTCCGGG GGACAAGACA
     4621 TCCCTGTTTA ATATTTAAAC AGCAGTGTTC CCAAACTGGG TTCTTATATC CCTTGCTCTG
     4681 GTCAACCAGG TTGCAGGGTT TCCTGTCCTC ACAGGAACGA AGTCCCTAAA GAAACAGTGG
     4741 CAGCCAGGTT TAGCCCCGGA ATTGACTGGA TTCCTTTTTT AGGGCCCATT GGTctagaCG
     4801 TGACCGAGCT TGTCTGCCCG CGCAGTCCAC TAGACAGACG CGCAGAGGTC GGGCGGGCCT
     4861 AGGGCCAATT TCCCATGGTT CCCTTCATTT GCATATATGA TGTAATAAGG TTATGGAGAC
     4921 TATTAAACTT AGCCCTAATC AAACTATATG ATGATAATGT GTGTGGTACA AAAGGTCATA
     4981 ACTTATTATA TACTTTGAAA CTTAAAAAAG GGTTACAGTT TAGTCACCAT AACTGTAAAA
     5041 TTTTTTCTAT TCTTAGCTTT ATATAGTTCT TGAGAGGCCA TGTTTATGGC AGAAGCTATT
     5101 AATTCGCGGG TTTaAGAGCT AaGCTGGAAA CAGCATAGCA AGTTtAAATA AGGCTAGTCC
     5161 GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTCCAGTCTG ACTATCACGA
     5221 TGgatccgac gcgccatctc taggcccgcg ccggccccct cgcacggact tgtgggagaa
     5281 gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta actatagagg
     5341 cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac attttacatg
     5401 ataggcttgg atttctataa GAGATACAAA TACTAAATTA TTATTTTAAA aaacagcaca
     5461 aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa Gtatcccttg
     5521 gagaaCCAcc tTGTTGGTCC TTTGTATGCC GAAAAAGTTT GAGAGCTAAG CAGAAAGCTG
     5581 CATAGCAAGT TCAAATAAGG CTAGTCCGTA CACAACTTGA AAAAGTGGCA GCCGAGTCGG
     5641 CTGCTTTTTT TGCTCGAATC TACACTCAGC TATGGCGCGC CCCAAGGTCG GGCAGGAAGA
     5701 GGgcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     5761 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     5821 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     5881 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgT GGAAAGccaG
     5941 AAACAtgGTT GGATCATAAC GATATCTCGT TTCAGAGCTA AGCACAAGAG TGCATAGCAA
     6001 GTTGAAATAA GGCTAGTCCG TTTACAACTT GAAAAAGTGG CACCCGAGTC GGGTGCTTTT
     6061 TTTCtcgagt actaggatcc attaggcggc cgCGTTTAAA CATGGCTTTT TCCCCGTATC
     6121 CCCCCAGGTG TCTGCAGGCT CAAAGAGCAG CGAGAAGCGT TCAGAGGAAA GCGATCCCGT
     6181 GCCACCTTCC CCGTGCCCGG GCTGTCCCCG CACGCTGCCG GCTCGGGGAT GCGGGGGGAG
     6241 CGCCGGACCG GAGCGGAGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT
     6301 AATTACATCC CTGGGGGCTT TGGGGGGGGG CTGTCCCTGA TATCTATAAC AAGAAAATAT
     6361 ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA TCGTATGAGT
     6421 TAAATCTTAA AAGTCACGTA AAAGATAATC ATGCGTCATT TTGACTCACG CGGTCGTTAT
     6481 AGTTCAAAAT CAGTGACACT TACCGCATTG ACAAGCACGC CTCACGGGAG CTCCAAGCGG
     6541 CGACTGAGAT GTCCTAAATG CACAGCGACG GATTCGCGCT ATTTAGAAAG AGAGAGCAAT
     6601 ATTTCAAGAA TGCATGCGTC AATTTTACGC AGACTATCTT TCTAGGGTTA ATCTAGCTGC
     6661 ATCAGGATCA TATCGTCGGG TCTTTTTTCC GGCTCAGTCA TCGCCCAAGC TGGCGCTATC
     6721 TGGGCATCGG GGAGGAAGAA GCCCGTGCCT TTTCCCGCGA GGTTGAAGCG GCATGGAAAG
     6781 AGTTTGCCGA GGATGACTGC TGCTGCATTG ACGTTGAGCG AAAACGCACG TTTACCATGA
     6841 TGATTCGGGA AGGTGTGGCC ATGCACGCCT TTAACGGTGA ACTGTTCGTT CAGGCCACCT
     6901 GGGATACCAG TTCGTCGCGG CTTTTCCGGA CACAGTTCCG GATGGTCAGC CCGAAGCGCA
     6961 TCAGCAACCC GAACAATACC GGCGACAGCC GGAACTGCCG TGCCGGTGTG CAGATTAATG
     7021 ACAGCGGTGC GGCGCTGGGA TATTACGTCA GCGAGGACGG GTATCCTGGC TGGATGCCGC
     7081 AGAAATGGAC ATGGATACCC CGTGAGTTAC CCGGCGGGCG CGCTTGGCGT AATCATGGTC
     7141 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG
     7201 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT
     7261 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG
     7321 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA
     7381 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT
     7441 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
     7501 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     7561 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     7621 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     7681 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     7741 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     7801 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     7861 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     7921 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     7981 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     8041 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     8101 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     8161 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     8221 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     8281 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     8341 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     8401 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC
     8461 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     8521 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     8581 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     8641 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     8701 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     8761 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     8821 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     8881 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     8941 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     9001 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     9061 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     9121 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCAT
//