LOCUS       pDYT002:_pPCT60_piggyBA 9159 bp ds-DNA     circular     10-MAY-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pdyt002-ppct60_piggybac_ucoe-ef1a-mch-pct62-ts1-1.gb
FEATURES             Location/Qualifiers
     misc_feature    1257..1276
                     /label="BII(R)"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
                     /note="ApEinfo_revcolor: #0080ff"
     misc_feature    1319..1381
                     /label="5'ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     misc_feature    1332..1362
                     /label="31bp spacer"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_fwdcolor="#0080c0"
                     /note="ApEinfo_revcolor: #0080c0"
     misc_feature    1557..1788
                     /label="Core Insulator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    1802..1814
                     /label="EF1 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    1815..1841
                     /label="MC_gbpr65"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     chromatin       1838..2078
                     /label="minUCOE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     misc_feature    complement(1852..1860)
                     /label="CTCF binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    2027..2040
                     /label="CTCF binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    2079..2517
                     /label="UCOE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     mRNA            2436..2517
                     /label="CBX3 mRNA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    2450..2458
                     /label="missing in actual plasmid"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    2519..2597
                     /label="EFS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    2598..3696
                     /label="hEF1alpha promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    3697..3716
                     /label="MC_gbpr64"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3828..3994
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    3846..3846
                     /label="gbPrimerEnd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    3996..4538
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    4556..4865
                     /label="Target Site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4577..4590
                     /label="intBC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="ApEinfo_revcolor: #b4abac"
     misc_feature    4651..4674
                     /label="site1:Bao_ADE2.d"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    4705..4705
                     /label="Bri1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    4706..4728
                     /label="site2:Bri1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    4759..4782
                     /label="site3:whiteB"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /note="ApEinfo_revcolor: #cc66ff"
     misc_feature    4843..4864
                     /label="10x capture site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    4866..4996
                     /label="SV40 polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    5209..5259
                     /label="DY007 gib"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    5244..5258
                     /label="bU6-2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    5259..5533
                     /label="bU6-2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    5468..5487
                     /label="PSE"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    5503..5510
                     /label="TATA box"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    5523..5533
                     /label="BstXI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="ApEinfo_revcolor: #ff0000"
     misc_feature    5534..5554
                     /label="whtB"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    5545..5545
                     /label="Mismatch #2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5555..5640
                     /label="sgRNA F + E constant region"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="ApEinfo_revcolor: #ff0000"
     PCR_primer      complement(5625..5640)
                     /label="BA100"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    5641..5647
                     /label="polyT-track"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="ApEinfo_revcolor: #ff0000"
     PCR_primer      complement(5648..5667)
                     /label="BA278"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    complement(5648..5667)
                     /label="triple-assembly-1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     promoter        complement(5668..5981)
                     /label="ANTIPARALLEL - mouse U6 - modified"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    5862..5865
                     /label="loxP"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #c7b0e3"
     misc_feature    5982..6001
                     /label="site1:Bao_ADE2.d"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6002..6007
                     /label="lower stem part 1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6002..6016
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6006..6006
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    6010..6016
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6017..6024
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6025..6031
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6025..6057
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6036..6041
                     /label="lower stem part 2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6042..6057
                     /label="Nexus"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     misc_feature    6058..6096
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6097..6115
                     /label="BA281"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    complement(6097..6115)
                     /label="BA280"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     promoter        complement(6116..6391)
                     /label="hU6 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    complement(6392..6392)
                     /label="ANTIPARALLEL eGFP-NT2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     misc_feature    6393..6413
                     /label="bri1 (perfect)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6410..6410
                     /label="mimsatch I"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="ApEinfo_revcolor: #ff0000"
     misc_feature    6414..6419
                     /label="lower stem part 1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6414..6428
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6422..6428
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6429..6436
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6437..6443
                     /label="upper stem and bulge"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6437..6469
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6448..6453
                     /label="lower stem part 2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6454..6466
                     /label="Nexus"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     misc_feature    6467..6469
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    6470..6508
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6488..6488
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    6498..6498
                     /label="change"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    complement(6509..6561)
                     /label="DY008 gib"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    6517..6537
                     /label="oMJ0042"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    6553..6783
                     /label="Core Insulator"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
                     /note="ApEinfo_revcolor: #ff0080"
     misc_feature    7058..7092
                     /label="3'ITR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
                     /note="ApEinfo_revcolor: #0000ff"
     primer_bind     complement(7611..7630)
                     /label="pLac(F)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #84b0dc"
     CDS             complement(8747..445)
                     /label="Amp"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #84b0dc"
     CDS             complement(8747..445)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
ORIGIN
        1 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
       61 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
      121 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
      181 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
      241 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
      301 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
      361 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
      421 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
      481 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
      541 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA ATATTTTGTT
      601 AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG
      661 CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG
      721 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA
      781 TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG
      841 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA
      901 GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
      961 GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT
     1021 ACAGGGCGCG TCCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG
     1081 GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG
     1141 GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCCTC
     1201 GTTCATTCAC GTTTTTGAAC CCGTGGAGGA CGGGCAGACT CGCGGTGCAA ATGTGTTTTA
     1261 CAGCGTGATG GAGCAGATGA AGATGCTCGA CACGCTGCAG AACACGCAGC TAGATTAACC
     1321 CTAGAAAGAT AATCATATTG TGACGTACGT TAAAGATAAT CATGTGTAAA ATTGACGCAT
     1381 GTGTTTTATC GGTCTGTATA TCGAGGTTTA TTTATTAATT TGAATAGATA TTAAGTTTTA
     1441 TTATATTTAC ACTTACATAC TAATAATAAA TTCAACAAAC AATTTATTTA TGTTTATTTA
     1501 TTTATTAAAA AAAACAAAAA CTCAAAATTT CTTCTATAAA GTAACAAAAC TTTTATGAGG
     1561 GACAGCCCCC CCCCAAAGCC CCCAGGGATG TAATTACGTC CCTCCCCCGC TAGGGGGCAG
     1621 CAGCGAGCCG CCCGGGGCTC CGCTCCGGTC CGGCGCTCCC CCCGCATCCC CGAGCCGGCA
     1681 GCGTGCGGGG ACAGCCCGGG CACGGGGAAG GTGGCACGGG ATCGCTTTCC TCTGAACGCT
     1741 TCTCGCTGCT CTTTGAGCCT GCAGACACCT GGGGGGATAC GGGGAAAAGG CCTCCACGGC
     1801 CAAGGATCTG CGATatcgat aagcttgata tcgaattcgg gaggtggtcc ctgcagttac
     1861 gccaatgata acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc
     1921 caactcggcg gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt
     1981 attgttcacg ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta
     2041 caggagagtt cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc
     2101 cccctccccc agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc
     2161 ggagtccggt tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag
     2221 actccggtca ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc
     2281 cacgccgcga acggtaagtg ccgcggtcgt cggcgcctcc gccctccccc tagggcccca
     2341 attcccagcg ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc
     2401 ccttcgtggc cgcccggcgt gggcggtgcc acccctcccc ccggcggccc ccctgcagcc
     2461 gcgcgcagct cccggctccc tcccccttcg gatgtggctt gagctgtagg cgcggagggg
     2521 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     2581 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     2641 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     2701 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     2761 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     2821 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     2881 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     2941 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     3001 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     3061 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     3121 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     3181 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     3241 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     3301 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     3361 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     3421 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     3481 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     3541 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     3601 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     3661 ggttcaaagt ttttttcttc catttcaggt gtcgtggaat tctcacgcgt ctgcagCTAG
     3721 CTATGGTGAG CAAGGGCGAC ACTGCTCGAT CCGCTCGCAC CTGACTGCTA CGTGACTACG
     3781 CTGATGTACA GATCCATACT GCATGTTCAT TGACGATCTA CCTAGCTatg gtgagcaagg
     3841 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     3901 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3961 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     4021 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4081 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4141 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4201 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4261 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4321 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4381 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4441 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4501 gccactccac cggcggcatg gacgagctgt acaagtaaTG AATTAATtaa GTCACGAATC
     4561 CAGCTAGCTG TGCAGCNNNN NNNNNNNNNN ATTCAACTGC AGTAATGCTA CCTCGTACTC
     4621 ACGCTTTCCA AGTGCTTGGC GTCGCATCTC GGTCCTTTGT ACGCCGAAAA ATGGCCTGAC
     4681 AACTAAGCTA CGGCACGCTG CCATGTTGGG TCATAACGAT ATCTCTGGTT CATCCGTGAC
     4741 CGAACATGTC ATGGAGTAGC AGGAGCTATT AATTCGCGGA GGACAATGCG GTTCGTAGTC
     4801 ACTGTCTTCC GCAATCGTCC ATCGCTCCTG CAGGTGTTAA CTGCTCACCT ATTAGCGGCT
     4861 AAGGAAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
     4921 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
     4981 TATCTTATCA TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTCATCT
     5041 GGTCTCCCTT CCGGGGGACA AGACATCCCT GTTTAATATT TAAACAGCAG TGTTCCCAAA
     5101 CTGGGTTCTT ATATCCCTTG CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG TCCTCACAGG
     5161 AACGAAGTCC CTAAAGAAAC AGTGGCAGCC AGGTTTAGCC CCGGAATTGA CTGGATTCCT
     5221 TTTTTAGGGC CCATTGGTct agaCGTGACC GAGCTTGTCT GCCCGCGCAG TCCACTAGAC
     5281 AGACGCGCAG AGGTCGGGCG GGCCTAGGGC CAATTTCCCA TGGTTCCCTT CATTTGCATA
     5341 TATGATGTAA TAAGGTTATG GAGACTATTA AACTTAGCCC TAATCAAACT ATATGATGAT
     5401 AATGTGTGTG GTACAAAAGG TCATAACTTA TTATATACTT TGAAACTTAA AAAAGGGTTA
     5461 CAGTTTAGTC ACCATAACTG TAAAATTTTT TCTATTCTTA GCTTTATATA GTTCTTGAGA
     5521 GGCCATGTTT ATGGCAGGAG CTATCAATTC GCGGGTTTaA GAGCTAaGCT GGAAACAGCA
     5581 TAGCAAGTTt AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC
     5641 TTTTTTTCCA GTCTGACTAT CACGATGgat ccgacgcgcc atctctaggc ccgcgccggc
     5701 cccctcgcac ggacttgtgg gagaagctcg gctactcccc tgccccggtt aatttgcata
     5761 taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata atctgttctt
     5821 tttaatacta gctacatttt acatgatagg cttggatttc tataaGAGAT ACAAATACTA
     5881 AATTATTATT TTAAAaaaca gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt
     5941 gttttgagac tataaGtatc ccttggagaa CCAcctTGTT GGTCCTTTGT ATGCCGAAAA
     6001 AGTTTGAGAG CTAAGCAGAA AGCTGCATAG CAAGTTCAAA TAAGGCTAGT CCGTACACAA
     6061 CTTGAAAAAG TGGCAGCCGA GTCGGCTGCT TTTTTTGCTC GAATCTACAC TCAGCTATGG
     6121 CGCGCCCCAA GGTCGGGCAG GAAGAGGgcc tatttcccat gattccttca tatttgcata
     6181 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     6241 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     6301 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     6361 ctttatatat cttgTGGAAA GccaGAAACA tGGTTGGGTC ATAACGATAC CTCGTTTCAG
     6421 AGCTAAGCAC AAGAGTGCAT AGCAAGTTGA AATAAGGCTA GTCCGTTTAC AACTTGAAAA
     6481 AGTGGCACCC GAGTCGGGTG CTTTTTTTCt cgagtactag gatccattag gcggccgCGT
     6541 TTAAACATGG CTTTTTCCCC GTATCCCCCC AGGTGTCTGC AGGCTCAAAG AGCAGCGAGA
     6601 AGCGTTCAGA GGAAAGCGAT CCCGTGCCAC CTTCCCCGTG CCCGGGCTGT CCCCGCACGC
     6661 TGCCGGCTCG GGGATGCGGG GGGAGCGCCG GACCGGAGCG GAGCCCCGGG CGGCTCGCTG
     6721 CTGCCCCCTA GCGGGGGAGG GACGTAATTA CATCCCTGGG GGCTTTGGGG GGGGGCTGTC
     6781 CCTGATATCT ATAACAAGAA AATATATATA TAATAAGTTA TCACGTAAGT AGAACATGAA
     6841 ATAACAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC ACGTAAAAGA TAATCATGCG
     6901 TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG ACACTTACCG CATTGACAAG
     6961 CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT AAATGCACAG CGACGGATTC
     7021 GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT GCGTCAATTT TACGCAGACT
     7081 ATCTTTCTAG GGTTAATCTA GCTGCATCAG GATCATATCG TCGGGTCTTT TTTCCGGCTC
     7141 AGTCATCGCC CAAGCTGGCG CTATCTGGGC ATCGGGGAGG AAGAAGCCCG TGCCTTTTCC
     7201 CGCGAGGTTG AAGCGGCATG GAAAGAGTTT GCCGAGGATG ACTGCTGCTG CATTGACGTT
     7261 GAGCGAAAAC GCACGTTTAC CATGATGATT CGGGAAGGTG TGGCCATGCA CGCCTTTAAC
     7321 GGTGAACTGT TCGTTCAGGC CACCTGGGAT ACCAGTTCGT CGCGGCTTTT CCGGACACAG
     7381 TTCCGGATGG TCAGCCCGAA GCGCATCAGC AACCCGAACA ATACCGGCGA CAGCCGGAAC
     7441 TGCCGTGCCG GTGTGCAGAT TAATGACAGC GGTGCGGCGC TGGGATATTA CGTCAGCGAG
     7501 GACGGGTATC CTGGCTGGAT GCCGCAGAAA TGGACATGGA TACCCCGTGA GTTACCCGGC
     7561 GGGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
     7621 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
     7681 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
     7741 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
     7801 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
     7861 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
     7921 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     7981 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     8041 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     8101 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     8161 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     8221 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     8281 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     8341 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     8401 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     8461 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     8521 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
     8581 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     8641 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
     8701 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
     8761 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
     8821 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
     8881 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
     8941 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
     9001 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
     9061 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
     9121 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAA
//