LOCUS       pPSV39-UGI              6293 bp    DNA     circular SYN 10-MAR-2022
DEFINITION  Cloning vector pBR322, complete genome.
ACCESSION   urn.local...ab-etraq8p
KEYWORDS    ampicillin resistance, beta-lactamase, cloning vector, drug;
            resistance protein, origin of replication, plasmid, tetracycline;
            resistance.
SOURCE      
  ORGANISM  .
REFERENCE   [1]
  AUTHORS   Sutcliffe and J.G.
  TITLE     Nucleotide sequence of the ampicillin resistance gene of
            Escherichia coli plasmid pBR322.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 75, 8, 3737-3741, (1978) MEDLINE
            79012484
REFERENCE   [2]
  AUTHORS   Reed, R.R., Young, R.A., Steitz, J.A., Grindley, N.D. & Guyer and
            M.S.
  TITLE     Transposition of the Escherichia coli insertion element gamma
            generates a five-base-pair repeat.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 76, 10, 4882-4886, (1979) MEDLINE
            80056597
REFERENCE   [3]
  AUTHORS   Covarrubias, L., Cervantes, L., Covarrubias, A., Soberon, X.,
            Vichido, I., Blanco, A., Kupersztoch-Portnoy, Y.M. & Bolivar and F.
  TITLE     Construction and characterization of new cloning vehicles. V.
            Mobilization and coding properties of pBR322 and several deletion
            derivatives including pBR327 and pBR328.
  JOURNAL   Gene, 13, 1, 25-35, (1981) MEDLINE 81213464
REFERENCE   [4]
  AUTHORS   Marians, K.J., Soeller, W. & Zipursky and S.L.
  TITLE     Maximal limits of the Escherichia coli replication factor Y
            effector site sequences in pBR322 DNA.
  JOURNAL   J. Biol. Chem., 257, 10, 5656-5662, (1982) MEDLINE 82167416
REFERENCE   [5]
  AUTHORS   Brosius, J., Cate, R.L. & Perlmutter and A.P.
  TITLE     Precise location of two promoters for the beta-lactamase gene of
            pBR322. S1 mapping of ribonucleic acid isolated from Escherichia
            coli or synthesized in vitro.
  JOURNAL   J. Biol. Chem., 257, 15, 9205-9210, (1982) MEDLINE 82239419
REFERENCE   [6]
  AUTHORS   Van Dyke, M.W., Hertzberg, R.P. & Dervan and P.B.
  TITLE     Map of distamycin, netropsin, and actinomycin binding sites on
            heterogeneous DNA: DNA cleavage-inhibition patterns with
            methidiumpropyl-EDTA.Fe(II).
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 79, 18, 5470-5474, (1982) MEDLINE
            83039392
REFERENCE   [7]
  AUTHORS   Peden, K.W. & Nathans and D.
  TITLE     Local mutagenesis within deletion loops of DNA heteroduplexes.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 79, 23, 7214-7217, (1982) MEDLINE
            83117649
REFERENCE   [8]
  AUTHORS   Peden and K.W.
  TITLE     Revised sequence of the tetracycline-resistance gene of pBR322.
  JOURNAL   Gene, 22, 2-3, 277-280, (1983) MEDLINE 83263146
REFERENCE   [9]
  AUTHORS   Watabe, H., Iino, T., Kaneko, T., Shibata, T. & Ando and T.
  TITLE     A new class of site-specific endodeoxyribonucleases. Endo.Sce I
            isolated from a eukaryote, Saccharomyces cerevisiae.
  JOURNAL   J. Biol. Chem., 258, 8, 4663-4665, (1983) MEDLINE 83161053
REFERENCE   [10]
  AUTHORS   Livneh and Z.
  TITLE     Directed mutagenesis method for analysis of mutagen specificity:
            application to ultraviolet-induced mutagenesis.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 80, 1, 237-241, (1983) MEDLINE
            83117828
REFERENCE   [11]
  AUTHORS   Mascharak, P.K., Sugiura, Y., Kuwahara, J., Suzuki, T. & Lippard
            and S.J.
  TITLE     Alteration and activation of sequence-specific cleavage of DNA by
            bleomycin in the presence of the antitumor drug
            cis-diamminedichloroplatinum(II).
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 80, 22, 6795-6798, (1983) MEDLINE
            84070716
REFERENCE   [12]
  AUTHORS   Schultz, P.G. & Dervan and P.B.
  TITLE     Sequence-specific double-strand cleavage of DNA by
            penta-N-methylpyrrolecarboxamide-EDTA X Fe(II).
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 80, 22, 6834-6837, (1983) MEDLINE
            84070724
REFERENCE   [13]
  AUTHORS   Sutcliffe and J.G.
  JOURNAL   Unpublished
REFERENCE   [14]
  AUTHORS   Fuller, R.S., Funnell, B.E. & Kornberg and A.
  TITLE     The dnaA protein complex with the E. coli chromosomal replication
            origin (oriC) and other DNA sites.
  JOURNAL   Cell, 38, 3, 889-900, (1984) MEDLINE 85024881
REFERENCE   [15]
  AUTHORS   Lathe, R., Kieny, M.P., Skory, S. & Lecocq and J.P.
  TITLE     Linker tailing: unphosphorylated linker oligonucleotides for
            joining DNA termini.
  JOURNAL   DNA, 3, 2, 173-182, (1984) MEDLINE 84207440
REFERENCE   [16]
  AUTHORS   Heusterspreute, M. & Davison and J.
  TITLE     Restriction site bank vectors. II. DNA sequence analysis of plasmid
            pJRD158.
  JOURNAL   DNA, 3, 3, 259-268, (1984) MEDLINE 84260952
REFERENCE   [17]
  AUTHORS   Abarzua, P., Soeller, W. & Marians and K.J.
  TITLE     Mutational analysis of primosome assembly sites. I. Distinct
            classes of mutants in the pBR322 Escherichia coli factor Y DNA
            effector sequences.
  JOURNAL   J. Biol. Chem., 259, 22, 14286-14292, (1984) MEDLINE 85054885
REFERENCE   [18]
  AUTHORS   Soeller, W., Abarzua, P. & Marians and K.J.
  TITLE     Mutational analysis of primosome assembly sites. II. Role of
            secondary structure in the formation of active sites.
  JOURNAL   J. Biol. Chem., 259, 22, 14293-14300, (1984) MEDLINE 85054886
REFERENCE   [19]
  AUTHORS   Sutcliffe and J.G.
  TITLE     Complete nucleotide sequence of the Escherichia coli plasmid
            pBR322.
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol., 43, 77-90, (1979) MEDLINE
            80002802
REFERENCE   [20]
  AUTHORS   Van Dyke, M.M. & Dervan and P.B.
  TITLE     Echinomycin binding sites on DNA.
  JOURNAL   Science, 225, 4667, 1122-1127, (1984) MEDLINE 84300294
REFERENCE   [21]
  AUTHORS   Watson and N.
  TITLE     A new revision of the sequence of plasmid pBR322.
  JOURNAL   Gene, 70, 2, 399-403, (1988) MEDLINE 89108024
REFERENCE   [22]
  AUTHORS   Gilbert and W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
COMMENT     Database Reference:                                                
              GENBANK    SYNPBR322                                             
              Accession:  J01749 K00005 L08654 M10282 M10283 M10286 M10356     
            M10784 M10785 M10786 M33694 V01119                                 
            ------------                                                       
             The circular sequence is numbered such that 0 is the middle of the
            unique EcoRI site and the count increases first through the tet    
            genes, the pMB1 material, and finally through the Tn3 region.      
            Plasmid pBR322 contains ampicillin and tetracycline resistance     
            genes.  The ampicillin resistance gene (amp-r) is a penicillin     
            beta-lactamase. Promoters P1 and P3 are for the beta-lactamase     
            gene.                                                              
            P3 is the natural promoter, and P1 is artificially created by the  
            ligation of two different DNA fragments to create pBR322. P2 is in 
            the same region as P1, but it is on the opposite strand and        
            initiates transcription in the direction of the tetracycline       
            resistance gene.                                                   
             Mutational studies in the primosome assembly sites indicate four  
            types of mutations:  Class I having no effect on the activities    
            elicited by the DNA site and the bases involved are probably       
            spacers
             Class II requiring higher Mg-2+ concentrations than the   
            wild-type to be fully activated as factor Y ATPase effectors
             Class III co-inactivating both the ATPase effector and DNA
            replication    template activity of the site, indicating that they
            probably        represent essential contact points between factor Y
            and the DNA
                Class IV having a replication template activity intermediate
            that   of                                                          
                   class III and class II mutant DNAs.                         
                    Specific sites within or near the origins of replication
            are       recognized by dnaA protein.  Without dnaA binding to the
            origin of  replication chromosomal replication is not possible
            [15]. pBR322    DNA                                                
                            contains two separate regions on opposite strands
            and close to the  origin of replication which, when in
            single-stranded form, can act  as effectors for the ATPase activity
            of E.coli replication factor Y [5].  Small fragments of DNA
            containing these sites when cloned in  an f1 phage vector act as
            origins of DNA replication allowing the   formation of
            complementary double-stranded DNA in                  
            rifampicin-resistant, dna[B,G,C]-dependent fashion in vitro [5].   
             The biological activity of echinomycin is thought to be related to
            the formation of complexes by intercalating with cellular DNA [20].
            Complete source information:                                       
             Plasmid pBR322 from E.coli [2],[1],[3],[6],[11],[8],[5],[7],[12], 
            [13],[10],[9],[14],[18],[19],[15],[20],[16]
             pBR322 DNA in pXf3     [4].                                       
                                     The following data and their annotation
            were supplied by Will      Gilbert under the auspices of the
            Curator Program.                    CROSSREFERENCE                 
                                                              #parent          
                                                               
            GenBank(50):pSC101C, GenBank(50):Trn3                            
            #offspring                                                         
              VecBase(3):pBR325, VecBase(3):pBR327,            
            VecBase(3):pBR328,                                                 
                              VecBase(3):pAT153, VecBase(3):pUC7,              
            VecBase(3):pJRD158,                                                
                              VecBase(3):PiVX, VecBase(3):PiAN7,               
            VecBase(3):pSP64,                                                  
                              VecBase(3):pSP65, VecBase(3):pGEM1,              
            VecBase(3):pGEM2,                                                  
                              VecBase(3):pGEM3, VecBase(3):pGEM4,              
            VecBase(3):pKK223,                                                 
                              VecBase(3):pLBU3, VecBase(3):pTrS3,              
            VecBase(3):pRSVNeo,                                                
                              VecBase(3):pSV2Cat, VecBase(3):M13mp9,           
            VecBase(3):pHC79,                                                  
                              VecBase(3):pV34, VecBase(3):pKTH601,             
            VecBase(3):pKTH604,                                                
                              VecBase(3):pKTH605, VecBase(3):pKTH606,          
            VecBase(3):YEp24,                                                  
                              VecBase(3):YIp5, VecBase(3):YRp17,               
            VecBase(3):pSP18,                                                  
                         VecBase(3):pSP19,                                     
                              VecBase(3):pSP6T3, VecBase(3):pSP6T719,          
            VecBase(3):pT712,                                                  
                              VecBase(3):pT713, VecBase(3):pT7T318,            
            VecBase(3):pT7T319,                                                
                              VecBase(3):pT7T3A18, VecBase(3):pT7T3A19,        
            VecBase(3):pEX1,                                                   
                              VecBase(3):pEX2, VecBase(3):pEX3,                
            VecBase(3):pCKSP6,                                                 
                              VecBase(3):pACYC177, VecBase(3):pKO1,            
            VecBase(3):pKO2,                                                   
                         VecBase(3):pKM1,                                      
                              VecBase(3):pKM2, VecBase(3):pMBL1,               
            VecBase(3):pMBL604,                                                
                              VecBase(3):pMC1511, VecBase(3):pMC1871,          
            VecBase(3):pAA37X,                                                 
                              VecBase(3):pUR278, VecBase(3):pUR288,            
            VecBase(3):pUR289,                                                 
                              VecBase(3):pUR290, VecBase(3):pUR291,            
            VecBase(3):pUR292,                                                 
                              VecBase(3):pUR222.
FEATURES             Location/Qualifiers
     promoter        11..41
                     /created_by="larry"
                     /label="lacUV5 promoter"
     gene            95..349
                     /created_by="fo"
                     /Color="#ff77ff"
                     /modified_by="larry"
                     /label="ugi"
     gene            1002..2084
                     /gene="lacI"
                     /note="[Rev]"
                     /Color="#ff7400"
                     /modified_by="larry"
                     /label="lacI"
     gene            2345..2878
                     /note="aacC1* (gentR up mutant #2) [Rev]"
                     /Color="#5500cc"
                     /modified_by="larry"
                     /label="GmR"
     oriT            3005..3114
                     /created_by="larry"
                     /modified_by="larry"
     repeat_region   complement(3443..3480)
                     /note="[Rev]"
     rep_origin      3490..4109
                     /created_by="larry"
                     /modified_by="larry"
                     /label="ori pMB1"
     gene            complement(4522..4713)
                     /note="ROP protein [Rev]"
                     /Color="#bbffff"
                     /modified_by="larry"
                     /label="rop"
     repeat_region   5211..5218
                     /note="[Rev]"
     repeat_region   complement(5222..5229)
                     /note="[Rev]"
     gene            complement(5231..5896)
                     /note="repA [Rev]"
                     /Color="#cdeb8b"
                     /modified_by="larry"
                     /label="repA"
     repeat_region   complement(5660..5669)
                     /note="[Rev]"
     repeat_region   complement(5704..5713)
                     /note="[Rev]"
     protein_bind    complement(6078..6085)
                     /bound_moiety="DnaA protein; note=/function=Replication"
                     /note="[Rev]"
     rep_origin      6078..9
                     /created_by="larry"
                     /Color="#ddffff"
                     /modified_by="larry"
                     /label="oriV"
     repeat_region   complement(6115..6131)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   complement(6137..6153)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   complement(6159..6175)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   complement(6181..6197)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   6205..6217
                     /note="[Rev]"
     repeat_region   complement(6223..6235)
                     /note="repeat_region [Rev]"
ORIGIN      
        1 cactacgtgc tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat
       61 aacaatttca gaattcgagc tcacgggagg aaagatgacg aatctcagcg acatcatcga
      121 gaaagaaacg gggaaacaac tggtgatcca ggaaagcatc ctgatgctcc ccgaggaggt
      181 cgaggaagtc atcggcaata aaccggagtc ggatatcctg gtccataccg cgtatgacga
      241 aagcacggac gagaacgtca tgctcctcac gtcggatgcg ccggaatata aaccgtgggc
      301 cctcgtgatc caagatagca atggcgagaa caaaatcaag atgctctaat ctagatatac
      361 agatattgaa atgaatagat taggaaaatg agctagagtc gacctgcagg catgcaagct
      421 ttagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgat cgcccttccc
      481 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
      541 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
      601 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      661 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      721 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      781 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      901 gacaataacc ctgataaatg cttcaataat attaatggag caaaaccttt cgcggtatgg
      961 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
     1021 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     1081 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     1141 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     1201 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     1261 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     1321 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     1381 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     1441 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     1501 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     1561 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     1621 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     1681 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     1741 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     1801 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgtcaa
     1861 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     1921 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     1981 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2041 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     2101 gtgagaatat tacgcgtaat tctcgaattg acataagcct gttcggttcg taaactgtaa
     2161 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2221 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct
     2281 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     2341 aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2401 aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc
     2461 catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca
     2521 acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct
     2581 tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt
     2641 tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag
     2701 gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc
     2761 ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa
     2821 gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca
     2881 attcgttcaa gccgagatcg gcttcccggc cgacgcgtca attccgtgat aggtgggctg
     2941 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     3001 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     3061 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     3121 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     3181 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     3241 gcggaaaacg gaattggacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4261 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4321 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4381 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     4441 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4501 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     4561 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     4621 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     4681 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     4741 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     4801 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     4861 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     4921 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     4981 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     5041 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     5101 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     5161 cacgatcatg cgcacccgtg gaaattaatt aattgcagaa aggcaggccg ggccgtggtg
     5221 gccacggcct ctaggccaga tccagcggca tctgggttag tcgagcgcgg gccgcttccc
     5281 atgtctcacc agggcgagcc tgtttcgcga tctcagcatc tgaaatcttc ccggccttgc
     5341 gcttcgctgg ggccttaccc accgccttgg cgggcttctt cggtccaaaa ctgaacaaca
     5401 gatgtgtgac cttgcgcccg gtctttcgct gcgcccactc cacctgtagc gggctgtgct
     5461 cgttgatctg cgtcacggct ggatcaagca ctcgcaactt gaagtccttg atcgagggat
     5521 accggccttc cagttgaaac cactttcgca gctggtcaat ttctatttcg cgctggccga
     5581 tgctgtccca ttgcatgagc agctcgtaaa gcctgatcgc gtgggtgctg tccatcttgg
     5641 ccacgtcagc caaggcgtat ttggtgaact gtttggtgag ttccgtcagg tacggcagca
     5701 tgtctttggt gaacctgagt tctacacggc cctcaccctc ccggtagatg attgtttgca
     5761 cccagccggt aatcatcaca ctcggtcttt tccccttgcc attgggctct tgggttaacc
     5821 ggacttcccg ccgtttcagg cgcagggccg cttctttgag ctggttgtag gaagattcga
     5881 tagggacacc cgccatcgtc gctatgtcct ccgccgtcac tgaatacatc acttcatcgg
     5941 tgacaggctc gctcctcttc acctggctaa tacaggccag aacgatccgc tgttcctgaa
     6001 cactgaggcg atacgcggcc tcgaccaggg cattgctttt gtaaaccatt gggggtgagg
     6061 ccacgttcga cattccttgt gtataagggg acactgtatc tgcgtcccac aatacaacaa
     6121 atccgtccct ttacaacaac aaatccgtcc cttcttaaca acaaatccgt cccttaatgg
     6181 caacaaatcc gtcccttttt aaactctaca ggccacggat tacgtggcct gtagacgtcc
     6241 taaaaggttt aaaagggaaa aggaagaaaa gggtggaaac gcaaaaaacg cac
//
