LOCUS       pPSV39-dddAI            6410 bp    DNA     circular SYN 17-MAY-2022
DEFINITION  Cloning vector pBR322, complete genome.
ACCESSION   urn.local...2u-etqufli
KEYWORDS    ampicillin resistance, beta-lactamase, cloning vector, drug;
            resistance protein, origin of replication, plasmid, tetracycline;
            resistance.
SOURCE      
  ORGANISM  .
REFERENCE   [1]
  AUTHORS   Sutcliffe and J.G.
  TITLE     Nucleotide sequence of the ampicillin resistance gene of
            Escherichia coli plasmid pBR322.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 75, 8, 3737-3741, (1978) MEDLINE
            79012484
REFERENCE   [2]
  AUTHORS   Reed, R.R., Young, R.A., Steitz, J.A., Grindley, N.D. & Guyer and
            M.S.
  TITLE     Transposition of the Escherichia coli insertion element gamma
            generates a five-base-pair repeat.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 76, 10, 4882-4886, (1979) MEDLINE
            80056597
REFERENCE   [3]
  AUTHORS   Covarrubias, L., Cervantes, L., Covarrubias, A., Soberon, X.,
            Vichido, I., Blanco, A., Kupersztoch-Portnoy, Y.M. & Bolivar and F.
  TITLE     Construction and characterization of new cloning vehicles. V.
            Mobilization and coding properties of pBR322 and several deletion
            derivatives including pBR327 and pBR328.
  JOURNAL   Gene, 13, 1, 25-35, (1981) MEDLINE 81213464
REFERENCE   [4]
  AUTHORS   Marians, K.J., Soeller, W. & Zipursky and S.L.
  TITLE     Maximal limits of the Escherichia coli replication factor Y
            effector site sequences in pBR322 DNA.
  JOURNAL   J. Biol. Chem., 257, 10, 5656-5662, (1982) MEDLINE 82167416
REFERENCE   [5]
  AUTHORS   Brosius, J., Cate, R.L. & Perlmutter and A.P.
  TITLE     Precise location of two promoters for the beta-lactamase gene of
            pBR322. S1 mapping of ribonucleic acid isolated from Escherichia
            coli or synthesized in vitro.
  JOURNAL   J. Biol. Chem., 257, 15, 9205-9210, (1982) MEDLINE 82239419
REFERENCE   [6]
  AUTHORS   Van Dyke, M.W., Hertzberg, R.P. & Dervan and P.B.
  TITLE     Map of distamycin, netropsin, and actinomycin binding sites on
            heterogeneous DNA: DNA cleavage-inhibition patterns with
            methidiumpropyl-EDTA.Fe(II).
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 79, 18, 5470-5474, (1982) MEDLINE
            83039392
REFERENCE   [7]
  AUTHORS   Peden, K.W. & Nathans and D.
  TITLE     Local mutagenesis within deletion loops of DNA heteroduplexes.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 79, 23, 7214-7217, (1982) MEDLINE
            83117649
REFERENCE   [8]
  AUTHORS   Peden and K.W.
  TITLE     Revised sequence of the tetracycline-resistance gene of pBR322.
  JOURNAL   Gene, 22, 2-3, 277-280, (1983) MEDLINE 83263146
REFERENCE   [9]
  AUTHORS   Watabe, H., Iino, T., Kaneko, T., Shibata, T. & Ando and T.
  TITLE     A new class of site-specific endodeoxyribonucleases. Endo.Sce I
            isolated from a eukaryote, Saccharomyces cerevisiae.
  JOURNAL   J. Biol. Chem., 258, 8, 4663-4665, (1983) MEDLINE 83161053
REFERENCE   [10]
  AUTHORS   Livneh and Z.
  TITLE     Directed mutagenesis method for analysis of mutagen specificity:
            application to ultraviolet-induced mutagenesis.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 80, 1, 237-241, (1983) MEDLINE
            83117828
REFERENCE   [11]
  AUTHORS   Mascharak, P.K., Sugiura, Y., Kuwahara, J., Suzuki, T. & Lippard
            and S.J.
  TITLE     Alteration and activation of sequence-specific cleavage of DNA by
            bleomycin in the presence of the antitumor drug
            cis-diamminedichloroplatinum(II).
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 80, 22, 6795-6798, (1983) MEDLINE
            84070716
REFERENCE   [12]
  AUTHORS   Schultz, P.G. & Dervan and P.B.
  TITLE     Sequence-specific double-strand cleavage of DNA by
            penta-N-methylpyrrolecarboxamide-EDTA X Fe(II).
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A., 80, 22, 6834-6837, (1983) MEDLINE
            84070724
REFERENCE   [13]
  AUTHORS   Sutcliffe and J.G.
  JOURNAL   Unpublished
REFERENCE   [14]
  AUTHORS   Fuller, R.S., Funnell, B.E. & Kornberg and A.
  TITLE     The dnaA protein complex with the E. coli chromosomal replication
            origin (oriC) and other DNA sites.
  JOURNAL   Cell, 38, 3, 889-900, (1984) MEDLINE 85024881
REFERENCE   [15]
  AUTHORS   Lathe, R., Kieny, M.P., Skory, S. & Lecocq and J.P.
  TITLE     Linker tailing: unphosphorylated linker oligonucleotides for
            joining DNA termini.
  JOURNAL   DNA, 3, 2, 173-182, (1984) MEDLINE 84207440
REFERENCE   [16]
  AUTHORS   Heusterspreute, M. & Davison and J.
  TITLE     Restriction site bank vectors. II. DNA sequence analysis of plasmid
            pJRD158.
  JOURNAL   DNA, 3, 3, 259-268, (1984) MEDLINE 84260952
REFERENCE   [17]
  AUTHORS   Abarzua, P., Soeller, W. & Marians and K.J.
  TITLE     Mutational analysis of primosome assembly sites. I. Distinct
            classes of mutants in the pBR322 Escherichia coli factor Y DNA
            effector sequences.
  JOURNAL   J. Biol. Chem., 259, 22, 14286-14292, (1984) MEDLINE 85054885
REFERENCE   [18]
  AUTHORS   Soeller, W., Abarzua, P. & Marians and K.J.
  TITLE     Mutational analysis of primosome assembly sites. II. Role of
            secondary structure in the formation of active sites.
  JOURNAL   J. Biol. Chem., 259, 22, 14293-14300, (1984) MEDLINE 85054886
REFERENCE   [19]
  AUTHORS   Sutcliffe and J.G.
  TITLE     Complete nucleotide sequence of the Escherichia coli plasmid
            pBR322.
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol., 43, 77-90, (1979) MEDLINE
            80002802
REFERENCE   [20]
  AUTHORS   Van Dyke, M.M. & Dervan and P.B.
  TITLE     Echinomycin binding sites on DNA.
  JOURNAL   Science, 225, 4667, 1122-1127, (1984) MEDLINE 84300294
REFERENCE   [21]
  AUTHORS   Watson and N.
  TITLE     A new revision of the sequence of plasmid pBR322.
  JOURNAL   Gene, 70, 2, 399-403, (1988) MEDLINE 89108024
REFERENCE   [22]
  AUTHORS   Gilbert and W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
COMMENT     Database Reference:                                                
              GENBANK    SYNPBR322                                             
              Accession:  J01749 K00005 L08654 M10282 M10283 M10286 M10356     
            M10784 M10785 M10786 M33694 V01119                                 
            ------------                                                       
             The circular sequence is numbered such that 0 is the middle of the
            unique EcoRI site and the count increases first through the tet    
            genes, the pMB1 material, and finally through the Tn3 region.      
            Plasmid pBR322 contains ampicillin and tetracycline resistance     
            genes.  The ampicillin resistance gene (amp-r) is a penicillin     
            beta-lactamase. Promoters P1 and P3 are for the beta-lactamase     
            gene.                                                              
            P3 is the natural promoter, and P1 is artificially created by the  
            ligation of two different DNA fragments to create pBR322. P2 is in 
            the same region as P1, but it is on the opposite strand and        
            initiates transcription in the direction of the tetracycline       
            resistance gene.                                                   
             Mutational studies in the primosome assembly sites indicate four  
            types of mutations:  Class I having no effect on the activities    
            elicited by the DNA site and the bases involved are probably       
            spacers
             Class II requiring higher Mg-2+ concentrations than the   
            wild-type to be fully activated as factor Y ATPase effectors
             Class III co-inactivating both the ATPase effector and DNA
            replication    template activity of the site, indicating that they
            probably        represent essential contact points between factor Y
            and the DNA
                Class IV having a replication template activity intermediate
            that   of                                                          
                   class III and class II mutant DNAs.                         
                    Specific sites within or near the origins of replication
            are       recognized by dnaA protein.  Without dnaA binding to the
            origin of  replication chromosomal replication is not possible
            [15]. pBR322    DNA                                                
                            contains two separate regions on opposite strands
            and close to the  origin of replication which, when in
            single-stranded form, can act  as effectors for the ATPase activity
            of E.coli replication factor Y [5].  Small fragments of DNA
            containing these sites when cloned in  an f1 phage vector act as
            origins of DNA replication allowing the   formation of
            complementary double-stranded DNA in                  
            rifampicin-resistant, dna[B,G,C]-dependent fashion in vitro [5].   
             The biological activity of echinomycin is thought to be related to
            the formation of complexes by intercalating with cellular DNA [20].
            Complete source information:                                       
             Plasmid pBR322 from E.coli [2],[1],[3],[6],[11],[8],[5],[7],[12], 
            [13],[10],[9],[14],[18],[19],[15],[20],[16]
             pBR322 DNA in pXf3     [4].                                       
                                     The following data and their annotation
            were supplied by Will      Gilbert under the auspices of the
            Curator Program.                    CROSSREFERENCE                 
                                                              #parent          
                                                               
            GenBank(50):pSC101C, GenBank(50):Trn3                            
            #offspring                                                         
              VecBase(3):pBR325, VecBase(3):pBR327,            
            VecBase(3):pBR328,                                                 
                              VecBase(3):pAT153, VecBase(3):pUC7,              
            VecBase(3):pJRD158,                                                
                              VecBase(3):PiVX, VecBase(3):PiAN7,               
            VecBase(3):pSP64,                                                  
                              VecBase(3):pSP65, VecBase(3):pGEM1,              
            VecBase(3):pGEM2,                                                  
                              VecBase(3):pGEM3, VecBase(3):pGEM4,              
            VecBase(3):pKK223,                                                 
                              VecBase(3):pLBU3, VecBase(3):pTrS3,              
            VecBase(3):pRSVNeo,                                                
                              VecBase(3):pSV2Cat, VecBase(3):M13mp9,           
            VecBase(3):pHC79,                                                  
                              VecBase(3):pV34, VecBase(3):pKTH601,             
            VecBase(3):pKTH604,                                                
                              VecBase(3):pKTH605, VecBase(3):pKTH606,          
            VecBase(3):YEp24,                                                  
                              VecBase(3):YIp5, VecBase(3):YRp17,               
            VecBase(3):pSP18,                                                  
                         VecBase(3):pSP19,                                     
                              VecBase(3):pSP6T3, VecBase(3):pSP6T719,          
            VecBase(3):pT712,                                                  
                              VecBase(3):pT713, VecBase(3):pT7T318,            
            VecBase(3):pT7T319,                                                
                              VecBase(3):pT7T3A18, VecBase(3):pT7T3A19,        
            VecBase(3):pEX1,                                                   
                              VecBase(3):pEX2, VecBase(3):pEX3,                
            VecBase(3):pCKSP6,                                                 
                              VecBase(3):pACYC177, VecBase(3):pKO1,            
            VecBase(3):pKO2,                                                   
                         VecBase(3):pKM1,                                      
                              VecBase(3):pKM2, VecBase(3):pMBL1,               
            VecBase(3):pMBL604,                                                
                              VecBase(3):pMC1511, VecBase(3):pMC1871,          
            VecBase(3):pAA37X,                                                 
                              VecBase(3):pUR278, VecBase(3):pUR288,            
            VecBase(3):pUR289,                                                 
                              VecBase(3):pUR290, VecBase(3):pUR291,            
            VecBase(3):pUR292,                                                 
                              VecBase(3):pUR222.
FEATURES             Location/Qualifiers
     promoter        11..41
                     /created_by="larry"
                     /label="lacUV5 promoter"
     gene            95..466
                     /locus_tag="I35_RS34175"
                     /modified_by="larry"
                     /label="dddAI"
     gene            1119..2201
                     /gene="lacI"
                     /note="[Rev]"
                     /Color="#ff7400"
                     /modified_by="larry"
                     /label="lacI"
     gene            2462..2995
                     /note="aacC1* (gentR up mutant #2) [Rev]"
                     /Color="#5500cc"
                     /modified_by="larry"
                     /label="GmR"
     oriT            3122..3231
                     /created_by="larry"
                     /modified_by="larry"
     repeat_region   complement(3560..3597)
                     /note="[Rev]"
     rep_origin      3607..4226
                     /created_by="larry"
                     /modified_by="larry"
                     /label="ori pMB1"
     gene            complement(4639..4830)
                     /note="ROP protein [Rev]"
                     /Color="#bbffff"
                     /modified_by="larry"
                     /label="rop"
     repeat_region   5328..5335
                     /note="[Rev]"
     repeat_region   complement(5339..5346)
                     /note="[Rev]"
     gene            complement(5348..6013)
                     /note="repA [Rev]"
                     /Color="#cdeb8b"
                     /modified_by="larry"
                     /label="repA"
     repeat_region   complement(5777..5786)
                     /note="[Rev]"
     repeat_region   complement(5821..5830)
                     /note="[Rev]"
     protein_bind    complement(6195..6202)
                     /bound_moiety="DnaA protein; note=/function=Replication"
                     /note="[Rev]"
     rep_origin      6195..9
                     /created_by="larry"
                     /Color="#ddffff"
                     /modified_by="larry"
                     /label="oriV"
     repeat_region   complement(6232..6248)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   complement(6254..6270)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   complement(6276..6292)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   complement(6298..6314)
                     /note="[Rev]"
                     /label="Iteron; note=/rpt_type=direct"
     repeat_region   6322..6334
                     /note="[Rev]"
     repeat_region   complement(6340..6352)
                     /note="repeat_region [Rev]"
ORIGIN      
        1 cactacgtgc tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat
       61 aacaatttca gaattcgagc tcacgggagg aaagatgtac gcagacgatt tcgacggcga
      121 aattgagatc gatgaggttg atagcttggt cgagttcctc agcagaagac ctgcatttga
      181 cgcaaataat tttgttttga cgtttgagga aagtggtttt cctcaattga atatttttgc
      241 aaaaaacgat atcgccgttg tttattatat ggacatcggc gagaatttcg tttcgaaagg
      301 caattctgcg tcaggtggta cggagaagtt ttacgagaat aagcttggcg gggaagtaga
      361 cctgtcgaaa gattgcgttg tgagtaaaga gcaaatgatt gaagcggcaa agcagttttt
      421 tgctacgaag caacggcccg agcaactgac atggagcgag ttgtaatcta gatatacaga
      481 tattgaaatg aatagattag gaaaatgagc tagagtcgac ctgcaggcat gcaagcttta
      541 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgatcgc ccttcccaac
      601 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      661 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      721 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      781 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      841 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      901 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      961 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1021 aataaccctg ataaatgctt caataatatt aatggagcaa aacctttcgc ggtatggcat
     1081 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
     1141 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     1201 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     1261 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     1321 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     1381 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     1441 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     1501 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     1561 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     1621 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     1681 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     1741 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     1801 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     1861 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     1921 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgtcaacca
     1981 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     2041 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     2101 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2161 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2221 agaatattac gcgtaattct cgaattgaca taagcctgtt cggttcgtaa actgtaatgc
     2281 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2341 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg
     2401 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     2461 gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg
     2521 tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat
     2581 gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct actcccaaca
     2641 tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc
     2701 tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaagtttga
     2761 gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc accggaggca
     2821 gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc ttggtgctta
     2881 tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt
     2941 gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaacaatt
     3001 cgttcaagcc gagatcggct tcccggccga cgcgtcaatt ccgtgatagg tgggctgccc
     3061 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     3121 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     3181 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     3241 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     3301 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     3361 gaaaacggaa ttggacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4261 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4321 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4381 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4441 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     4501 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     4561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4621 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     4681 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     4741 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     4801 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     4861 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     4921 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     4981 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     5041 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     5101 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     5161 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     5221 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     5281 gatcatgcgc acccgtggaa attaattaat tgcagaaagg caggccgggc cgtggtggcc
     5341 acggcctcta ggccagatcc agcggcatct gggttagtcg agcgcgggcc gcttcccatg
     5401 tctcaccagg gcgagcctgt ttcgcgatct cagcatctga aatcttcccg gccttgcgct
     5461 tcgctggggc cttacccacc gccttggcgg gcttcttcgg tccaaaactg aacaacagat
     5521 gtgtgacctt gcgcccggtc tttcgctgcg cccactccac ctgtagcggg ctgtgctcgt
     5581 tgatctgcgt cacggctgga tcaagcactc gcaacttgaa gtccttgatc gagggatacc
     5641 ggccttccag ttgaaaccac tttcgcagct ggtcaatttc tatttcgcgc tggccgatgc
     5701 tgtcccattg catgagcagc tcgtaaagcc tgatcgcgtg ggtgctgtcc atcttggcca
     5761 cgtcagccaa ggcgtatttg gtgaactgtt tggtgagttc cgtcaggtac ggcagcatgt
     5821 ctttggtgaa cctgagttct acacggccct caccctcccg gtagatgatt gtttgcaccc
     5881 agccggtaat catcacactc ggtcttttcc ccttgccatt gggctcttgg gttaaccgga
     5941 cttcccgccg tttcaggcgc agggccgctt ctttgagctg gttgtaggaa gattcgatag
     6001 ggacacccgc catcgtcgct atgtcctccg ccgtcactga atacatcact tcatcggtga
     6061 caggctcgct cctcttcacc tggctaatac aggccagaac gatccgctgt tcctgaacac
     6121 tgaggcgata cgcggcctcg accagggcat tgcttttgta aaccattggg ggtgaggcca
     6181 cgttcgacat tccttgtgta taaggggaca ctgtatctgc gtcccacaat acaacaaatc
     6241 cgtcccttta caacaacaaa tccgtccctt cttaacaaca aatccgtccc ttaatggcaa
     6301 caaatccgtc cctttttaaa ctctacaggc cacggattac gtggcctgta gacgtcctaa
     6361 aaggtttaaa agggaaaagg aagaaaaggg tggaaacgca aaaaacgcac
//
