LOCUS       pLV-GFP-Sec61b                 7722 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             4497..5213
                     /note="pLannotate"
                     /label="AcGFP1"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    5526..6114
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      7009..7437
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3901..4204
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     CDS             5229..5519
                     /note="pLannotate"
                     /label="Sec61β"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    3033..3266
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             6201..6434
                     /note="pLannotate"
                     /label="3' LTR (ΔU3)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        1961..2187
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4205..4408
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             2188..2368
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     rep_origin      6667..6802
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    2415..2540
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    6506..6627
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        7490..7594
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(7595..7722,1..733)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      904..1492
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    1780..1801
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(6823..6841)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1915..1933
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1854..1870
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             6849..7004
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    3762..3878
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="98.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             2524..2887
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3643..3743
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     promoter        1820..1846
                     /note="pLannotate"
                     /label="lac promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="87.1"
                     /fragment="True"
                     /other="promoter"
     CDS             1675..1767
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             6131..6199
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="91.3"
                     /match_length="23.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
       61 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      121 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      181 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      241 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      301 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      361 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      421 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      481 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      541 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      601 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      661 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      721 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1801 ttaggcaccc caggctttcc actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1861 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     1921 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     1981 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     2041 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     2101 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     2161 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     2221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2341 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2401 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2461 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2521 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2581 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2641 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2701 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2761 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2821 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2881 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     2941 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3001 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3061 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3121 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3181 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3241 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     3301 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     3361 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     3421 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     3481 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     3541 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     3601 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     3661 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     3721 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     3781 attggggggt atagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     3841 aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt gggagttccg
     3901 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3961 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4021 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4081 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4141 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4201 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4261 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4321 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4381 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     4441 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc accggtatgg
     4501 tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg
     4561 atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca
     4621 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg
     4681 tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc
     4741 acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg
     4801 aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat accctggtga
     4861 atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg ggcaataaga
     4921 tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc aagaatggca
     4981 tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc
     5041 actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc
     5101 tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctact
     5161 tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtccggac
     5221 tcagatctat gcctggtccg acccccagtg gcactaacgt gggatcctca gggcgctctc
     5281 ccagcaaagc agtggccgcc cgggcggcgg gatccactgt ccggcagagg aaaaatgcca
     5341 gctgtgggac aaggagtgca ggccgcacaa cctcggcagg caccgggggg atgtggcgat
     5401 tctacacaga agattcacct gggctcaaag ttggccctgt tccagtattg gttatgagtc
     5461 ttctgttcat cgcttctgta tttatgttgc acatttgggg caagtacact cgttcgtagg
     5521 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     5941 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6121 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6181 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6241 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6421 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6481 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6601 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6661 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6721 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6781 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6841 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6901 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6961 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     7021 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7081 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7141 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7201 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7261 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7321 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7381 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7441 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7501 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7561 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7621 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7681 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg gg
//
