LOCUS       Rtn2b-mNeon                 6115 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(3917..4711)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(5944..6115,1..536))
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      5236..5664
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(4746..5103)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(2243..2546)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(2039..2242)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(5698..5819)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(5105..5209)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(573..1988)
                     /note="pLannotate"
                     /label="Rtn2"
                     /database="swissprot"
                     /identity="84.2"
                     /match_length="100.2"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2721..3309)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3638..3685)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(3744..3896)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1989..2016)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(544..570)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5842..5925)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ctccggtagc gcttgccatt tccagtggtg taactccact taaaggtact gatgatggtt
       61 ttgtcgttgg ggtaagtctt cttcgacctg caccagtccg cagcggtcag cgagttggtc
      121 atcacaggac cgtcagcagg gaaaccagtc cccttcacct gggcctctcc tttgatgtgg
      181 cttccctcgt aggtgtagcg gtagttaaca gtaagggagg caccatcttc aaactgcatt
      241 gtgcgatgga cttggtagcc ggagccatct accatggcgg cctggaaagg cgacatcccg
      301 tcagggtagg gcaggtactg atggaagcca tacccgatat gagggaccag aatccagggg
      361 gagaactgga ggtcaccctt ggtggacttc aggtttaact cctcataacc atcatttgga
      421 ttgccggtgc cctgacccac catgtcaaag tccacaccgt tgatggagcc aaagatgtgt
      481 aactcatgtg tcgctgggag agaggccatg ttatcctcct cgcccttgct caccatggtg
      541 gcgaccggtg gatcccgggc ccgcggtacc cgttcggctt tggctttgga tccggagact
      601 gcggctgctg cagaggccag ggctccggtc cctgggattt tagctcggat cttagctttg
      661 atgtggctca actgattggt caccaacccc acatattggt cgatctgagc ctggtgctgc
      721 cggtacagca gggggatggt gaatagacca atcactccca gaatgagaag agtcaaacca
      781 ttgaagatgg cacccacgaa ggtcaagatg tagaagagga gggccagctt gagggaatcc
      841 acgaggtctt ctaccaggaa gaagtgccgc agctgcgtgg ccgccgagac cacgcgggag
      901 gtgatctggt gggacaaacg ttccgtctgc tcccgagtca gggtgaggtc cacatccagg
      961 taggcctgga aagggttggc tccatccccc cggtgcacgg cctgcagcac tttgcggtaa
     1021 accctgagag agatggtgcc gcagagcagc aacagagcca agtgcgcggc cacggacacg
     1081 atgctaaagt gcaggaggca gaggagggag accatcaggc ctgtgaagac cactcctgac
     1141 gtcctcgtgt ccttccagta cagcaggtcc gccactagaa ggcgtggctc caccgtgaat
     1201 tctaattggt ccgtgctatc gaggcactgt ccccttactg gctctcgctc cagtggcccc
     1261 cactgctttt cttcctcttc cagcaatggc tcttcgggcc cagagttcga atctcgcgat
     1321 cgggatgggg acggagtacc ggcctggggt gtcccagagc ccggactgag ctggggagtc
     1381 aagacctcgg gcgatgaggg ctgagcaagt cggagtcgta ggtccagttc ttccccagct
     1441 tctcctgtct ccaatctgtt gggttcttgg ggttcttcgt cttccagcgg ggtggagctg
     1501 ctggtggaag agtcctcccc ggatcccgtt ccccgggcca cccagcccag atggtccaac
     1561 cgaagcctca ggtcttccag agggcgctcg gatggaggcg cggtgtcagg atcaccccgt
     1621 cgtccaggct ccggggattg gctcaggctg gggatgctct ccaagctgtc gcccaggctg
     1681 ggctgagggt gctggtctcg tggttccgag actgagcggc cctgggggcg ggggcggcgg
     1741 gcagttgaat ccctgcggcc cccggagccc actacaccat caaaggcgat gtaggagaag
     1801 gtcagctccc ggggggtgcc ccagtcctgc gacgtggtct cctcctcgtc ctcctctgag
     1861 aattcccggg ctgtgtgcag ctctcgaaaa tcagagtcgt cgttccctcc ttctgtggaa
     1921 tcaggagttg aggaggctgt agacggagct tctttgcagt gggcgaagac cggcaggacc
     1981 tgccccatct cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag
     2041 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     2101 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     2161 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     2221 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     2281 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     2341 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     2401 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     2461 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     2521 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     2581 ccgtaattga ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca
     2641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2881 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3121 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
     3481 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
     3541 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
     3601 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
     3661 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
     3721 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
     3781 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     3841 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     3901 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3961 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     4021 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     4081 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4141 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     4201 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     4261 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     4321 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     4381 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     4441 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     4501 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     4561 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4621 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4681 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     4741 cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4801 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     4861 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     4921 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4981 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     5041 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     5101 caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5221 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5281 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5581 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5641 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tacgccttaa
     5701 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     5761 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     5821 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     5881 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
     5941 ttacttgtac agctcgtcca tgcccatcac atcggtaaag gccttttgcc actccttgaa
     6001 gttgagctcg gtcttggagt gcttgagctc cgtcttacgg aacacgtaca tcggctggtt
     6061 cttcagatag ttagccgcca ttggcttggc aaaggtgtag gtggtccgcg cagtg
//
