LOCUS       Rtn2b-GFP                 6118 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2021..2815)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4045..4764)
                     /note="pLannotate"
                     /label="AcGFP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3340..3768
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2850..3207)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(347..650)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(143..346)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(3802..3923)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3209..3313)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(4795..6118,1..92))
                     /note="pLannotate"
                     /label="Rtn2"
                     /database="swissprot"
                     /identity="84.0"
                     /match_length="100.2"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(825..1413)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(1742..1789)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(1848..2000)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(93..120)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(4766..4792)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3946..4029)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcgtcgttcc ctccttctgt ggaatcagga gttgaggagg ctgtagacgg agcttctttg
       61 cagtgggcga agaccggcag gacctgcccc atctcgagat ctgagtccgg tagcgctagc
      121 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
      181 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      241 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
      301 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
      361 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
      421 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
      481 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
      541 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
      601 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
      661 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta atgcatggcg
      721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1561 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     1621 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     1681 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     1741 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     1801 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     1861 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     1921 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     1981 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     2041 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     2101 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     2161 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     2221 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     2281 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     2341 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     2401 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     2461 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     2521 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     2581 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     2641 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     2701 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     2761 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     2821 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     2881 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     2941 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     3001 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     3061 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     3121 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     3181 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
     3241 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3301 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     3361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3541 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3661 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3781 taacgcttac aatttacgcc ttaagataca ttgatgagtt tggacaaacc acaactagaa
     3841 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3901 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3961 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     4021 attatgatct agagtcgcgg ccgctcactt gtacagctca tccatgccgt gggtgatggc
     4081 ggcggcggtc acgaagccga agtagatcat gtgatcgcgc ttctcgttgg ggtccttgga
     4141 cagggcgctc tgggtggaca ggtagtggtt atcgggcagc agcacagggc catcgccgat
     4201 gggggtattc tgctggtagt ggtcggccag ctgcacgctg ccatcctcga tgttgtggcg
     4261 gatcttgaag ttcaccttga tgccattctt ggccttgtcg gtcatgatgt acacattgtg
     4321 ggcgttgtag ttgtactcca tcttattgcc caggatgttg ccatcctcct tgaaatcggt
     4381 gccggtcagc tcgatgcgat tcaccagggt atcgccctcg aacttcacct cggcgcgcga
     4441 cttgtagttg ccgtcatcct cgaagaagat ggtgcgctcc tggatgtagc cctcaggcat
     4501 ggcgctcttg aagaagtcgt gctgcttcat gtgatcgggg tagcgtgaga agcactgcac
     4561 gccgtagctc agggtggtca ccagggtggg ccagggcaca ggcagcttgc cggtggtgca
     4621 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cgctcacgct
     4681 gaacttgtgg ccattcacat cgccattcag ctcgatcagg atgggcacga tgccggtgaa
     4741 cagctcggcg cccttgctca ccatgaccgg tggatcccgg gcccgcggta cccgttcggc
     4801 tttggctttg gatccggaga ctgcggctgc tgcagaggcc agggctccgg tccctgggat
     4861 tttagctcgg atcttagctt tgatgtggct caactgattg gtcaccaacc ccacatattg
     4921 gtcgatctga gcctggtgct gccggtacag cagggggatg gtgaatagac caatcactcc
     4981 cagaatgaga agagtcaaac cattgaagat ggcacccacg aaggtcaaga tgtagaagag
     5041 gagggccagc ttgagggaat ccacgaggtc ttctaccagg aagaagtgcc gcagctgcgt
     5101 ggccgccgag accacgcggg aggtgatctg gtgggacaaa cgttccgtct gctcccgagt
     5161 cagggtgagg tccacatcca ggtaggcctg gaaagggttg gctccatccc cccggtgcac
     5221 ggcctgcagc actttgcggt aaaccctgag agagatggtg ccgcagagca gcaacagagc
     5281 caagtgcgcg gccacggaca cgatgctaaa gtgcaggagg cagaggaggg agaccatcag
     5341 gcctgtgaag accactcctg acgtcctcgt gtccttccag tacagcaggt ccgccactag
     5401 aaggcgtggc tccaccgtga attctaattg gtccgtgcta tcgaggcact gtccccttac
     5461 tggctctcgc tccagtggcc cccactgctt ttcttcctct tccagcaatg gctcttcggg
     5521 cccagagttc gaatctcgcg atcgggatgg ggacggagta ccggcccggg gtgtcccaga
     5581 gcccggactg agctggggag tcaagacctc gggcgatgag ggctgagcaa gtcggagtcg
     5641 taggtccagt tcttccccag cttctcctgt ctccaatctg ttgggttctt ggggttcttc
     5701 gtcttccagc ggggtggagc tgctggtgga agagtcctcc ccggatcccg ttccccgggc
     5761 cacccagccc agatggtcca accgaagcct caggtcttcc agagggcgct cggatggagg
     5821 cgcggtgtca ggatcacccc gtcgtccagg ctccggggat tggctcaggc tggggatgct
     5881 ctccaagctg tcgcccaggc tgggctgagg gtgctggtct cgtggttccg agactgagcg
     5941 gccctggggg cgggggcggc gggcagttga atccctgcgg cccccggagc ccactacacc
     6001 atcaaaggcg atgtaggaga aggtcagctc ccggggggtg ccccagtcct gcgacgtggt
     6061 ctcctcctcg tcctcctctg agaattcccg ggctgtgtgc agctctcgaa aatcagag
//
