LOCUS       Rtn2b-mCherry                 6109 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2018..2812)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4042..4752)
                     /note="pLannotate"
                     /label="mCherry"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3337..3765
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2847..3204)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(344..647)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(140..343)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(3799..3920)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3206..3310)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(4783..6109,1..89))
                     /note="pLannotate"
                     /label="Rtn2"
                     /database="swissprot"
                     /identity="84.2"
                     /match_length="100.2"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(822..1410)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(1739..1786)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(1845..1997)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(90..117)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(4754..4780)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3943..4026)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcgttccctc cttctgtgga atcaggagtt gaggaggctg tagacggagc ttctttgcag
       61 tgggcgaaga ccggcaggac ctgccccatc tcgagatctg agtccggtag cgctagcgga
      121 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      181 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
      241 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      301 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
      361 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
      421 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
      481 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
      541 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
      601 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
      661 atgggctatg aactaatgac cccgtaattg attactatta ataactaatg catggcggta
      721 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      781 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      841 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      901 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      961 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1021 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1081 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1141 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1201 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1261 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1321 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1381 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1441 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1501 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1561 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
     1621 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
     1681 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
     1741 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     1801 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
     1861 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     1921 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     1981 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     2041 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     2101 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     2161 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     2221 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     2281 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     2341 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     2401 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     2461 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     2521 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     2581 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
     2641 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     2701 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     2761 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     2821 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
     2881 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     2941 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     3001 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     3061 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     3121 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     3181 cacagctggt tctttccgcc tcaggactct tcctttttca atattattga agcatttatc
     3241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     3361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3781 cgcttacaat ttacgcctta agatacattg atgagtttgg acaaaccaca actagaatgc
     3841 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3901 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3961 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     4021 atgatctaga gtcgcggccg cctacttgta cagctcgtcc atgccgccgg tggagtggcg
     4081 gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
     4141 caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt
     4201 ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc
     4261 gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt
     4321 cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
     4381 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
     4441 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg
     4501 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat
     4561 gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc
     4621 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
     4681 ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc
     4741 cttgctcacc atgaccggtg gatcccgggc ccgcggtacc cgttcggctt tggctttgga
     4801 tccggagact gcggctgctg cagaggccag ggctccggtc cctgggattt tagctcggat
     4861 cttagctttg atgtggctca actgattggt caccaacccc acatattggt cgatctgagc
     4921 ctggtgctgc cggtacagca gggggatggt gaatagacca atcactccca gaatgagaag
     4981 agtcaaacca ttgaagatgg cacccacgaa ggtcaagatg tagaagagga gggccagctt
     5041 gagggaatcc acgaggtctt ctaccaggaa gaagtgccgc agctgcgtgg ccgccgagac
     5101 cacgcgggag gtgatctggt gggacaaacg ttccgtctgc tcccgagtca gggtgaggtc
     5161 cacatccagg taggcctgga aagggttggc tccatccccc cggtgcacgg cctgcagcac
     5221 tttgcggtaa accctgagag agatggtgcc gcagagcagc aacagagcca agtgcgcggc
     5281 cacggacacg atgctaaagt gcaggaggca gaggagggag accatcaggc ctgtgaagac
     5341 cactcctgac gtcctcgtgt ccttccagta cagcaggtcc gccactagaa ggcgtggctc
     5401 caccgtgaat tctaattggt ccgtgctatc gaggcactgt ccccttactg gctctcgctc
     5461 cagtggcccc cactgctttt cttcctcttc cagcaatggc tcttcgggcc cagagttcga
     5521 atctcgcgat cgggatgggg acggagtacc ggcctggggt gtcccagagc ccggactgag
     5581 ctggggagtc aagacctcgg gcgatgaggg ctgagcaagt cggagtcgta ggtccagttc
     5641 ttccccagct tctcctgtct ccaatctgtt gggttcttgg ggttcttcgt cttccagcgg
     5701 ggtggagctg ctggtggaag agtcctcccc ggatcccgtt ccccgggcca cccagcccag
     5761 atggtccaac cgaagcctca ggtcttccag agggcgctcg gatggaggcg cggtgtcagg
     5821 atcaccccgt cgtccaggct ccggggattg gctcaggctg gggatgctct ccaagctgtc
     5881 gcccaggctg ggctgagggt gctggtctcg tggttccgag actgagcggc cctgggggcg
     5941 ggggcggcgg gcagttgaat ccctgcggcc cccggagccc actacaccat caaaggcgat
     6001 gtaggagaag gtcagctccc ggggggtgcc ccagtcctgc gacgtggtct cctcctcgtc
     6061 ctcctctgag aattcccggg ctgtgtgcag ctctcgaaaa tcagagtcg
//
