LOCUS       Rtn2-NTD1-60-mNeon                 4879 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2679..3473)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(4706..4879,1..534))
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3998..4426
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(3508..3865)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(1005..1308)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(801..1004)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(4460..4581)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3867..3971)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(1483..2071)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(2400..2447)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(2506..2658)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(571..750)
                     /note="pLannotate"
                     /label="Rtn2 (fragment)"
                     /database="swissprot"
                     /identity="96.7"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(751..778)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(542..568)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4604..4687)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ccggtagcgc ttgccatttc cagtggtgta actccactta aaggtactga tgatggtttt
       61 gtcgttgggg taagtcttct tcgacctgca ccagtccgca gcggtcagcg agttggtcat
      121 cacaggaccg tcagcaggga aaccagtccc cttcacctgg gcctctcctt tgatgtggct
      181 tccctcgtag gtgtagcggt agttaacagt aagggaggca ccatcttcaa actgcattgt
      241 gcgatggact tggtagccgg agccatctac catggcggcc tggaaaggcg acatcccgtc
      301 agggtagggc aggtactgat ggaagccata cccgatatga gggaccagaa tccaggggga
      361 gaactggagg tcacccttgg tggacttcag gtttaactcc tcataaccat catttggatt
      421 gccggtgccc tgacccacca tgtcaaagtc cacaccgttg atggagccaa agatgtgtaa
      481 ctcatgtgtc gctgggagag aggccatgtt atcctcctcg cccttgctca ccatggtggc
      541 gaccggtgga tcccgggccc gcggtacccg ccggggggtg ccccagtcct gcgacgtggt
      601 ctcctcctcg tcctcctctg agaattcccg ggctgtgtgc agctctcgaa aatcagagtc
      661 gtcgttccct ccttctgtgg aatcaggagt tgaggaggct gtagacggag cttctttgca
      721 gtgggcgaag accggcagga cctgccccat ctcgagatct gagtccggta gcgctagcgg
      781 atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg
      841 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
      901 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
      961 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     1021 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     1081 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     1141 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     1201 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     1261 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     1321 tatgggctat gaactaatga ccccgtaatt gattactatt aataactaat gcatggcggt
     1381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1921 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2221 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     2281 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     2341 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     2401 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     2461 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     2521 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     2581 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     2641 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     2701 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     2761 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     2821 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     2881 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     2941 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     3001 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     3061 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     3121 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     3181 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     3241 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     3301 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     3361 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     3421 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     3481 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     3541 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     3601 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     3661 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     3721 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     3781 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     3841 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     3901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     4021 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4081 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4141 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4201 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4261 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4321 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4381 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4441 acgcttacaa tttacgcctt aagatacatt gatgagtttg gacaaaccac aactagaatg
     4501 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4561 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     4621 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     4681 tatgatctag agtcgcggcc gcttacttgt acagctcgtc catgcccatc acatcggtaa
     4741 aggccttttg ccactccttg aagttgagct cggtcttgga gtgcttgagc tccgtcttac
     4801 ggaacacgta catcggctgg ttcttcagat agttagccgc cattggcttg gcaaaggtgt
     4861 aggtggtccg cgcagtgct
//
