LOCUS       Rtn2-NTD272-472-mNeon                 5314 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(63..857)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2090..2797)
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1382..1810
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(892..1249)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(3703..4006)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(3499..3702)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1844..1965)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(1251..1355)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(4181..4769)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2834..3436)
                     /note="pLannotate"
                     /label="Rtn2 (fragment)"
                     /database="swissprot"
                     /identity="90.5"
                     /match_length="42.7"
                     /fragment="True"
                     /other="CDS"
     polyA_signal    complement(5098..5145)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(join(5204..5314,1..42))
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3449..3476)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(2805..2831)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1988..2071)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
       61 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
      121 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
      181 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
      241 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
      301 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
      361 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
      421 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
      481 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
      541 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
      601 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
      661 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
      721 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
      781 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
      841 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
      901 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
      961 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     1021 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     1081 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     1141 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     1201 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     1261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1381 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     1681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     1741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     1801 aacgcgaatt ttaacaaaat attaacgctt acaatttacg ccttaagata cattgatgag
     1861 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1921 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1981 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2041 ctctacaaat gtggtatggc tgattatgat ctagagtcgc ggccgcttac ttgtacagct
     2101 cgtccatgcc catcacatcg gtaaaggcct tttgccactc cttgaagttg agctcggtct
     2161 tggagtgctt gagctccgtc ttacggaaca cgtacatcgg ctggttcttc agatagttag
     2221 ccgccattgg cttggcaaag gtgtaggtgg tccgcgcagt gctccggtag cgcttgccat
     2281 ttccagtggt gtaactccac ttaaaggtac tgatgatggt tttgtcgttg gggtaagtct
     2341 tcttcgacct gcaccagtcc gcagcggtca gcgagttggt catcacagga ccgtcagcag
     2401 ggaaaccagt ccccttcacc tgggcctctc ctttgatgtg gcttccctcg taggtgtagc
     2461 ggtagttaac agtaagggag gcaccatctt caaactgcat tgtgcgatgg acttggtagc
     2521 cggagccatc taccatggcg gcctggaaag gcgacatccc gtcagggtag ggcaggtact
     2581 gatggaagcc atacccgata tgagggacca gaatccaggg ggagaactgg aggtcaccct
     2641 tggtggactt caggtttaac tcctcataac catcatttgg attgccggtg ccctgaccca
     2701 ccatgtcaaa gtccacaccg ttgatggagc caaagatgtg taactcatgt gtcgctggga
     2761 gagaggccat gttatcctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg
     2821 cccgcggtac ccgttcggct ttggctttgg atccggagac tgcggctgct gcagaggcca
     2881 gggctccggt ccctgggatt ttagctcgga tcttagcttt gatgtggctc aactgattgg
     2941 tcaccaaccc cacatattgg tcgatctgag cctggtgctg ccggtacagc agggggatgg
     3001 tgaatagacc aatcactccc agaatgagaa gagtcaaacc attgaagatg gcacccacga
     3061 aggtcaagat gtagaagagg agggccagct tgagggaatc cacgaggtct tctaccagga
     3121 agaagtgccg cagctgcgtg gccgccgaga ccacgcggga ggtgatctgg tgggacaaac
     3181 gttccgtctg ctcccgagtc agggtgaggt ccacatccag gtaggcctgg aaagggttgg
     3241 ctccatcccc ccggtgcacg gcctgcagca ctttgcggta aaccctgaga gagatggtgc
     3301 cgcagagcag caacagagcc aagtgcgcgg ccacggacac gatgctaaag tgcaggaggc
     3361 agaggaggga gaccatcagg cctgtgaaga ccactcctga cgtcctcgtg tccttccagt
     3421 acagcaggtc cgccacttta ctccccatct cgagatctga gtccggtagc gctagcggat
     3481 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     3541 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     3601 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     3661 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     3721 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     3781 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     3841 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     3901 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     3961 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     4021 tgggctatga actaatgacc ccgtaattga ttactattaa taactaatgc atggcggtaa
     4081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4621 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4921 agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt
     4981 cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat
     5041 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa
     5101 caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc
     5161 cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact
     5221 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     5281 agcccaacct ttcatagaag gcggcggtgg aatc
//
