LOCUS       Rtn2-NTD272-472-GFP                 5317 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(133..927)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2157..2876)
                     /note="pLannotate"
                     /label="AcGFP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1452..1880
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(962..1319)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(3776..4079)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(3572..3775)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1914..2035)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(1321..1425)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(4254..4842)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2907..3509)
                     /note="pLannotate"
                     /label="Rtn2 (fragment)"
                     /database="swissprot"
                     /identity="90.5"
                     /match_length="42.7"
                     /fragment="True"
                     /other="CDS"
     polyA_signal    complement(5171..5218)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(join(5277..5317,1..112))
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3522..3549)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(2878..2904)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2058..2141)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
       61 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
      121 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
      181 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
      241 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
      301 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
      361 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
      421 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
      481 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
      541 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
      601 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
      661 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
      721 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
      781 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
      841 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
      901 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat
      961 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     1021 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     1081 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     1141 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     1201 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     1261 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg
     1321 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1441 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1501 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1561 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1621 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1681 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1741 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1801 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1861 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg ccttaagata
     1921 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1981 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2041 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2101 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc ggccgctcac
     2161 ttgtacagct catccatgcc gtgggtgatg gcggcggcgg tcacgaagcc gaagtagatc
     2221 atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc tctgggtgga caggtagtgg
     2281 ttatcgggca gcagcacagg gccatcgccg atgggggtat tctgctggta gtggtcggcc
     2341 agctgcacgc tgccatcctc gatgttgtgg cggatcttga agttcacctt gatgccattc
     2401 ttggccttgt cggtcatgat gtacacattg tgggcgttgt agttgtactc catcttattg
     2461 cccaggatgt tgccatcctc cttgaaatcg gtgccggtca gctcgatgcg attcaccagg
     2521 gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt tgccgtcatc ctcgaagaag
     2581 atggtgcgct cctggatgta gccctcaggc atggcgctct tgaagaagtc gtgctgcttc
     2641 atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc tcagggtggt caccagggtg
     2701 ggccagggca caggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
     2761 gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt ggccattcac atcgccattc
     2821 agctcgatca ggatgggcac gatgccggtg aacagctcgg cgcccttgct caccatgacc
     2881 ggtggatccc gggcccgcgg tacccgttcg gctttggctt tggatccgga gactgcggct
     2941 gctgcagagg ccagggctcc ggtccctggg attttagctc ggatcttagc tttgatgtgg
     3001 ctcaactgat tggtcaccaa ccccacatat tggtcgatct gagcctggtg ctgccggtac
     3061 agcaggggga tggtgaatag accaatcact cccagaatga gaagagtcaa accattgaag
     3121 atggcaccca cgaaggtcaa gatgtagaag aggagggcca gcttgaggga atccacgagg
     3181 tcttctacca ggaagaagtg ccgcagctgc gtggccgccg agaccacgcg ggaggtgatc
     3241 tggtgggaca aacgttccgt ctgctcccga gtcagggtga ggtccacatc caggtaggcc
     3301 tggaaagggt tggctccatc cccccggtgc acggcctgca gcactttgcg gtaaaccctg
     3361 agagagatgg tgccgcagag cagcaacaga gccaagtgcg cggccacgga cacgatgcta
     3421 aagtgcagga ggcagaggag ggagaccatc aggcctgtga agaccactcc tgacgtcctc
     3481 gtgtccttcc agtacagcag gtccgccact ttactcccca tctcgagatc tgagtccggt
     3541 agcgctagcg gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta
     3601 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     3661 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     3721 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     3781 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     3841 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     3901 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     3961 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     4021 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     4081 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactaa
     4141 tgcatggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4981 taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg
     5041 agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg
     5101 tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc
     5161 ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc
     5221 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg
     5281 tgggcgaaga actccagcat gagatccccg cgctgga
//
