LOCUS       pLV-Kif5b-Myc                 9609 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             173..3061
                     /note="pLannotate"
                     /label="KIF5B"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    3101..3689
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      4584..5012
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        9198..9501
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    8330..8563
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             3776..4009
                     /note="pLannotate"
                     /label="3' LTR (ΔU3)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        7258..7484
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        join(9502..9609,1..96)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             7485..7665
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     rep_origin      4242..4377
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    7712..7837
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    4081..4202
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5065..5169
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             5170..6030
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      6201..6789
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    7077..7098
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(4398..4416)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        7212..7230
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    7151..7167
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4424..4579
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    9059..9175
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="98.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             7821..8184
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             8940..9040
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     promoter        7117..7143
                     /note="pLannotate"
                     /label="lac promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="87.1"
                     /fragment="True"
                     /other="promoter"
     CDS             6972..7064
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3706..3774
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="91.3"
                     /match_length="23.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
       61 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      121 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaatggcgga
      181 cctggccgag tgcaacatca aagtgatgtg tcgcttcaga cctctcaacg agtctgaagt
      241 gaaccgcggc gacaagtaca tcgccaagtt tcagggagaa gacacggtcg tgatcgcgtc
      301 caagccttat gcatttgatc gggtgttcca gtcaagcaca tctcaagagc aagtgtataa
      361 tgactgtgca aagaagattg ttaaagatgt acttgaagga tataatggaa caatatttgc
      421 atatggacaa acatcctctg ggaagacaca cacaatggag ggtaaacttc atgatccaga
      481 aggcatggga attattccaa gaatagtgca agatattttt aattatattt actccatgga
      541 tgaaaatttg gaatttcata ttaaggtttc atattttgaa atatatttgg ataagataag
      601 ggacctgtta gatgtttcaa agaccaacct ttcagttcat gaagacaaaa accgagttcc
      661 ctatgtaaag gggtgcacag agcgttttgt atgtagtcca gatgaagtta tggataccat
      721 agatgaagga aaatccaaca gacatgtagc agttacaaat atgaatgaac atagctctag
      781 gagtcacagt atatttctta ttaatgtcaa acaagagaac acacaaacgg aacaaaagct
      841 gagtggaaaa ctttatctgg ttgatttagc tggtagtgaa aaggttagta aaactggagc
      901 tgaaggtgct gtgctggatg aagctaaaaa catcaacaag tcactttctg ctcttggaaa
      961 tgttatttct gctttggctg agggtagtac atatgttcca tatcgagata gtaaaatgac
     1021 aagaatcctt caagattcat taggtggcaa ctgtagaacc actattgtaa tttgctgctc
     1081 tccatcatca tacaatgagt ctgaaacaaa atctacactc ttatttggcc aaagggccaa
     1141 aacaattaag aacacagttt gtgtcaatgt ggagttaact gcagaacagt ggaaaaagaa
     1201 gtatgaaaaa gaaaaagaaa aaaataagat cctgcggaac actattcagt ggcttgaaaa
     1261 tgagctcaac agatggcgta atggggagac ggtgcctatt gatgaacagt ttgacaaaga
     1321 gaaagccaac ttggaagctt tcacagtgga taaagatatt actcttacca atgataaacc
     1381 agcaaccgca attggagtta taggaaattt tactgatgct gaaagaagaa agtgtgaaga
     1441 agaaattgct aaattataca aacagcttga tgacaaggat gaagaaatta accagcaaag
     1501 tcaactggta gagaaactga agacgcaaat gttggatcag gaggagcttt tggcatctac
     1561 cagaagggat caagacaata tgcaagctga gctgaatcgc cttcaagcag aaaatgatgc
     1621 ctctaaagaa gaagtgaaag aagttttaca ggccctagaa gaacttgctg tcaattatga
     1681 tcagaagtct caggaagttg aagacaaaac taaggaatat gaattgctta gtgatgaatt
     1741 gaatcagaaa tcggcaactt tagcgagtat agatgctgag cttcagaaac ttaaggaaat
     1801 gaccaaccac cagaaaaaac gagcagctga gatgatggca tctttactaa aagaccttgc
     1861 agaaatagga attgctgtgg gaaataatga tgtaaagcag cctgagggaa ctggcatgat
     1921 agatgaagag ttcactgttg caagactcta cattagcaaa atgaagtcag aagtaaaaac
     1981 catggtgaaa cgttgcaagc agttagaaag cacacaaact gagagcaaca aaaaaatgga
     2041 agaaaatgaa aaggagttag cagcatgtca gcttcgtatc tctcaacatg aagccaaaat
     2101 caagtcattg actgaatacc ttcaaaatgt ggaacaaaag aaaagacagt tggaggaatc
     2161 tgtcgatgcc ctcagtgaag aactagtcca gcttcgagca caagagaaag tccatgaaat
     2221 ggaaaaggag cacttaaata aggttcagac tgcaaatgaa gttaagcaag ctgttgaaca
     2281 gcagatccag agccatagag aaactcatca aaaacagatc agtagtttga gagatgaagt
     2341 agaagcaaaa gcaaaactta ttactgatct tcaagaccaa aaccagaaaa tgatgttaga
     2401 gcaggaacgt ctaagagtag aacatgagaa gttgaaagcc acagatcagg aaaagagcag
     2461 aaaactacat gaacttacgg ttatgcaaga tagacgagaa caagcaagac aagacttgaa
     2521 gggtttggaa gagacagtgg caaaagaact tcagacttta cacaacctgc gcaaactctt
     2581 tgttcaggac ctggctacaa gagttaaaaa gagtgctgag attgattctg atgacaccgg
     2641 aggcagcgct gctcagaagc aaaaaatctc ctttcttgaa aataatcttg aacagctcac
     2701 taaagtgcac aaacagttgg tacgtgataa tgcagatctc cgctgtgaac ttcctaagtt
     2761 ggaaaagcga cttcgagcta cagctgagag agtgaaagct ttggaatcag cactgaaaga
     2821 agctaaagaa aatgcatctc gtgatcgcaa acgctatcag caagaagtag atcgcataaa
     2881 ggaagcagtc aggtcaaaga atatggccag aagagggcat tctgcacaga ttgctaaacc
     2941 tattcgtccc gggcaacatc cagcagcttc tccaactcac ccaagtgcaa ttcgtggagg
     3001 aggtgcattt gttcagaaca gccagccagt ggcagtgcga ggtggaggag gcaaacaagt
     3061 ggaacaaaaa cttatttctg aagaagatct gtaagtcgac aatcaacctc tggattacaa
     3121 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3181 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3241 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3301 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3361 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3421 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3481 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     3541 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3601 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3661 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca
     3721 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3781 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     3841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     4021 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     4081 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4141 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4201 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     4261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4321 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4381 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     4441 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4501 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4561 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4621 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4681 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4741 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4801 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4861 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4921 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4981 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     5041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5161 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5221 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5281 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5341 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5401 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5461 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5521 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5581 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5641 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5701 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5761 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5821 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5881 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5941 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6001 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6961 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7021 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7081 gtgagttagc tcactcatta ggcaccccag gctttccact ttatgcttcc ggctcgtatg
     7141 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7201 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     7261 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     7321 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     7381 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     7441 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     7501 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7561 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7621 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7681 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     7741 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     7801 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     7861 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     7921 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     7981 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8041 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8101 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8161 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     8221 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     8281 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     8341 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     8401 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8461 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8521 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8581 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8641 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8701 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8761 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     8821 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     8881 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     8941 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     9001 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     9061 ttaaaagaaa aggggggatt ggggggtata gtgcagggga aagaatagta gacataatag
     9121 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga
     9181 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     9241 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     9301 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     9361 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     9421 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     9481 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     9541 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     9601 tggcaccaa
//
