LOCUS       pLV-Kif5c-Myc                 9603 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    2806..3394
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      4289..4717
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        8903..9206
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    8035..8268
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             3481..3714
                     /note="pLannotate"
                     /label="3' LTR (ΔU3)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        6963..7189
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        9207..9410
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             7190..7370
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     CDS             join(9499..9603,1..2766)
                     /note="pLannotate"
                     /label="KIF5C"
                     /database="swissprot"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3947..4082
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    7417..7542
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    3786..3907
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4770..4874
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4875..5735
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      5906..6494
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    6782..6803
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(4103..4121)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        6917..6935
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    6856..6872
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4129..4284
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    8764..8880
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="98.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             7526..7889
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             8645..8745
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     promoter        6822..6848
                     /note="pLannotate"
                     /label="lac promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="87.1"
                     /fragment="True"
                     /other="promoter"
     CDS             6677..6769
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3411..3479
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="91.3"
                     /match_length="23.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gatgagaccg tggtgatcgg gcaagggaag ccatatgtct tcgacagagt gctacctccc
       61 aacacgaccc aagagcaggt ttacaatgca tgtgcgaagc aaattgtcaa agatgtcctt
      121 gaaggttata acgggacgat ttttgcgtat gggcagactt catcaggaaa aacccacacc
      181 atggagggga agctgcatga cccccagctc atggggatca tcccacgaat tgcccatgat
      241 atctttgacc atatctactc catggatgag aacctggagt ttcacataaa ggtttcctat
      301 tttgagatct acttggacaa aataagggac ttacttgatg tatccaagac caacttggct
      361 gttcatgaag ataaaaacag agtcccgtat gtaaaggggt gcactgagcg gtttgtgtcg
      421 agccccgagg aagtcatgga tgtaatagat gaaggcaaag caaaccgaca cgtggctgtg
      481 acaaacatga atgaacacag ctctagaagt cacagtatct tcctgataaa tattaaacaa
      541 gagaatgtag agactgaaaa aaaactcagt gggaaacttt atttggttga tttggctggg
      601 agcgaaaagg tcagcaaaac tggtgccgag ggagctgttc ttgacgaagc taaaaatatc
      661 aataagtctt tgtctgctct tggaaatgtg atctctgctt tggcagaagg gacaaaaaca
      721 catgtgccat accgggacag caagatgact cggattcttc aggactcttt gggtgggaac
      781 tgcagaacca ccatcgtcat ttgctgttct ccttctgtct tcaatgaggc tgagaccaag
      841 tccacactga tgttcggaca gagagctaag accatcaaga atacagtctc tgtgaaccta
      901 gaactgacag cagaagaatg gaagaagaaa tatgaaaaag agaaagagaa aaacaagact
      961 ttgaagaatg ttatccagca tctggagatg gagctaaaca ggtggaggaa tggagaagct
     1021 gtgcctgagg atgaacagat cagtgccaag gaccagaaga acccggagcc ttgtgataac
     1081 acccccatca tagacaatat tgctcctgtt gttgctggca tctctacaga ggagaaagag
     1141 aagtacgatg aggagatctc cagtctctac agacaactgg atgacaagga tgatgaaatt
     1201 aaccagcaga gccagctggc tgaaaagctg aagcaacaga tgttggatca ggatgagctt
     1261 ttagcttcca caagaagaga ctatgagaag atacaggagg agctgacacg tctccagatt
     1321 gaaaatgagg cagccaagga tgaggtgaaa gaagttctcc aggccctgga ggagctggct
     1381 gtcaattatg accagaaatc acaggaagtg gaggataaga cccgggccaa tgagcagctg
     1441 acagacgagc tggcccagaa aacgactaca ttgacaacca cacagagaga gctgagccag
     1501 ctacaagagc ttagcaacca ccagaagaaa agggcaactg agatcctgaa tttgctgttg
     1561 aaagatctgg gggagatagg tggaattatt ggcaccaatg atgtgaaaac tttggcagat
     1621 gtgaatggag tcattgagga ggagtttacc atggcccgcc tgtacatcag caagatgaag
     1681 tcagaggtca agtccctggt gaaccgcagc aaacagctcg agagcgccca gatggactcc
     1741 aacaggaaga tgaatgccag cgagcgggag ctggcagcct gccagctgct catctcccag
     1801 cacgaagcca agatcaagtc tctgacagac tacatgcaga acatggaaca gaagaggagg
     1861 cagctagaag agtcccagga ctcgctcagc gaagagctgg caaagctccg agcccaggaa
     1921 aaaatgcacg aagtcagctt ccaggataag gagaaggaac atctgacgcg gttgcaggat
     1981 gctgaagaaa tgaagaaggc gctggagcag cagatggaga gccaccggga agctcaccag
     2041 aagcagctgt ccagactccg agacgaaatt gaggagaagc agaaaatcat tgatgagatt
     2101 cgggatttga atcagaaact gcaactggaa caggagaagc ttagttctga ttataacaag
     2161 ctgaaaatag aggaccaaga gagagaaatg aagctggaaa agctcttatt gctcaacgat
     2221 aaaagggaac aagccagaga agacctcaaa gggctggagg agacagtgtc tagagaattg
     2281 cagacactgc acaaccttcg gaaactcttt gtccaggatc tgaccacccg agttaaaaaa
     2341 agtgtggagt tggacaacga tgatggaggg ggcagtgctg cccagaagca gaaaatttcc
     2401 ttcttggaga ataacctgga gcagctcacc aaagttcaca agcagctggt ccgggacaac
     2461 gcagacctgc gctgtgaact gcccaagctg gagaagcggc tgcgtgccac ggcggagcgc
     2521 gtcaaggctc tggagagcgc gctgaaggag gccaaggaga acgccatgcg ggaccgtaag
     2581 cgctaccagc aggaggtgga tcgtatcaag gaggccgtgc gggccaagaa catggccaga
     2641 agggcccatt cagcccagat cgccaagccc atccgccccg gacactaccc ggcctcatct
     2701 ccaacggccg tccatgccat tcgaggggga ggaggcagct cttcaaattc cactcactac
     2761 cagaaagaac aaaaacttat ttctgaagaa gatctgtaag tcgacaatca acctctggat
     2821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3181 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3361 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa
     3421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3481 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     3721 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3781 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3901 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3961 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4021 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4081 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4261 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4501 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4621 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4681 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4741 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4861 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4921 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4981 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5041 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5101 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5161 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5221 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5281 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5341 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5401 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5461 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5521 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5581 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5641 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5701 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6661 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6721 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6781 ttaatgtgag ttagctcact cattaggcac cccaggcttt ccactttatg cttccggctc
     6841 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6901 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6961 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7021 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7081 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7141 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7381 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7441 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7501 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7561 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7621 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7681 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7741 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7801 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7861 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7921 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7981 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8041 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8101 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8161 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8221 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8281 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8341 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8401 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8461 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8521 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8581 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8641 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8701 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     8761 aacttttaaa agaaaagggg ggattggggg gtatagtgca ggggaaagaa tagtagacat
     8821 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     8881 atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8941 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     9001 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     9061 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     9121 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     9181 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     9241 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     9301 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     9361 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     9421 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
     9481 tctagaggat ccaccggtat ggcggatcca gccgaatgca gcatcaaagt gatgtgccgg
     9541 ttccggcccc tcaacgaagc ggagatcctc cgcggggaca aattcatccc caaatttaaa
     9601 ggc
//
