LOCUS       NpY-mNeon                 4947 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1953..2747)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3980..4687)
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3272..3700
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2782..3139)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(279..582)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(75..278)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(3734..3855)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3141..3245)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(4703..4947,1..46))
                     /note="pLannotate"
                     /label="NPY"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(757..1345)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(1674..1721)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(1780..1932)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3878..3961)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gggcgagggt cagtccggac agccccagtc gcttgttacc tagcatgcta gcggatctga
       61 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      121 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      181 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      241 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      301 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      361 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      421 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
      481 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
      541 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
      601 ctatgaacta atgaccccgt aattgattac tattaataac taatgcatgg cggtaatacg
      661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1501 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     1561 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     1621 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     1681 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     1741 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     1801 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     1861 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     1921 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     1981 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     2041 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     2101 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     2161 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     2221 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     2281 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     2341 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     2401 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     2461 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     2521 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     2581 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     2641 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     2701 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     2761 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     2821 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     2881 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     2941 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     3001 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     3061 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     3121 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     3181 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3241 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     3301 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3361 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3421 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3481 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3541 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3601 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3661 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3721 acaatttacg ccttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3781 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3841 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3901 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     3961 ctagagtcgc ggccgcttac ttgtacagct cgtccatgcc catcacatcg gtaaaggcct
     4021 tttgccactc cttgaagttg agctcggtct tggagtgctt gagctccgtc ttacggaaca
     4081 cgtacatcgg ctggttcttc agatagttag ccgccattgg cttggcaaag gtgtaggtgg
     4141 tccgcgcagt gctccggtag cgcttgccat ttccagtggt gtaactccac ttaaaggtac
     4201 tgatgatggt tttgtcgttg gggtaagtct tcttcgacct gcaccagtcc gcagcggtca
     4261 gcgagttggt catcacagga ccgtcagcag ggaaaccagt ccccttcacc tgggcctctc
     4321 ctttgatgtg gcttccctcg taggtgtagc ggtagttaac agtaagggag gcaccatctt
     4381 caaactgcat tgtgcgatgg acttggtagc cggagccatc taccatggcg gcctggaaag
     4441 gcgacatccc gtcagggtag ggcaggtact gatggaagcc atacccgata tgagggacca
     4501 gaatccaggg ggagaactgg aggtcaccct tggtggactt caggtttaac tcctcataac
     4561 catcatttgg attgccggtg ccctgaccca ccatgtcaaa gtccacaccg ttgatggagc
     4621 caaagatgtg taactcatgt gtcgctggga gagaggccat gttatcctcc tcgcccttgc
     4681 tcaccatggt ggcgaccggt gcccacattg cagggtcttc aagccgagtt ctgggaacat
     4741 tttctgtgct ttctctcatc aagaggtctg aaatcagtgt ctctgggctg gatcgttttc
     4801 catatctctg cctggtgatg aggttgatgt agtgtcgcag cgccgagtag tatctggcca
     4861 tgtcctccgc tggtgcgtcc tcgcccgggt tgtccggctt ggaggggtac gcctcggcca
     4921 gcgcacccag gcacacgagc agggaca
//
