LOCUS       mNeon-Vamp2                 5040 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2568..3362)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(4978..5040,1..645))
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3887..4315
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(3397..3754)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(894..1197)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(690..893)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(4349..4470)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3756..3860)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(1372..1960)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(4603..4947)
                     /note="pLannotate"
                     /label="VAMP2"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="99.1"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(2289..2336)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(2395..2547)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(4959..4977)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4493..4577)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ctcggtcttg gagtgcttga gctccgtctt acggaacacg tacatcggct ggttcttcag
       61 atagttagcc gccattggct tggcaaaggt gtaggtggtc cgcgcagtgc tccggtagcg
      121 cttgccattt ccagtggtgt aactccactt aaaggtactg atgatggttt tgtcgttggg
      181 gtaagtcttc ttcgacctgc accagtccgc agcggtcagc gagttggtca tcacaggacc
      241 gtcagcaggg aaaccagtcc ccttcacctg ggcctctcct ttgatgtggc ttccctcgta
      301 ggtgtagcgg tagttaacag taagggaggc accatcttca aactgcattg tgcgatggac
      361 ttggtagccg gagccatcta ccatggcggc ctggaaaggc gacatcccgt cagggtaggg
      421 caggtactga tggaagccat acccgatatg agggaccaga atccaggggg agaactggag
      481 gtcacccttg gtggacttca ggtttaactc ctcataacca tcatttggat tgccggtgcc
      541 ctgacccacc atgtcaaagt ccacaccgtt gatggagcca aagatgtgta actcatgtgt
      601 cgctgggaga gaggccatgt tatcctcctc gcccttgctc accatggtgg cgaccggtag
      661 cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
      721 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      781 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      841 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      901 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      961 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     1021 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     1081 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     1141 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     1201 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactaatg
     1261 catggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2101 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag
     2161 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta
     2221 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc
     2281 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat
     2341 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg
     2401 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     2461 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     2521 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     2581 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     2641 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     2701 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     2761 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     2821 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     2881 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     2941 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     3001 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     3061 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     3121 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     3181 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     3241 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     3301 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     3361 atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa
     3421 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     3481 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     3541 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     3601 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     3661 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     3721 acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca atattattga
     3781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3841 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     3901 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3961 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4021 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4081 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4141 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4201 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4261 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4321 aaaatattaa cgcttacaat ttacgcgtta agatacattg atgagtttgg acaaaccaca
     4381 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4441 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4501 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4561 atggctgatt atgatcagtt atctagatcc ggtggatcct taagtgctga agtaaactat
     4621 gatgatgatg aggatgatgg cgcaaatcac tcccaagatg atcatcatct tgaggttttt
     4681 ccaccagtat ttgcgcttga gcttggctgc gcttgtttca aactgggagg cccccgcctg
     4741 gagtgcatct gcacggtcgt ccagctccga cagcttctgg tctcgctcca ggaccttgtc
     4801 cacgttcacc ctcatgatgt ccaccacctc atccacctgg gcctgggtct gctgcagtct
     4861 cctgttactg gtgaggtttg gagggggtgc agggggacca ccctccccag ccggggcagc
     4921 agggggggcc gtggcagcgg tagcagacct gtccatgcct cgagatctga gtccggactt
     4981 gtacagctcg tccatgccca tcacatcggt aaaggccttt tgccactcct tgaagttgag
//
