LOCUS       pLV-mCherry-Stim1-wt                 9462 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    7290..7878
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      8773..9201
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3925..4228
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    3057..3290
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             7965..8198
                     /note="pLannotate"
                     /label="3' LTR (ΔU3)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        1985..2211
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4229..4432
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             2212..2392
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     rep_origin      8431..8566
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    2439..2564
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    8270..8391
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        9254..9358
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             5295..7280
                     /note="pLannotate"
                     /label="STIM1"
                     /database="swissprot"
                     /identity="98.6"
                     /match_length="96.6"
                     /fragment="False"
                     /other="CDS"
     CDS             join(9359..9462,1..757)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             4587..5294
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      928..1516
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    1804..1825
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(8587..8605)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1939..1957
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1878..1894
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             8613..8768
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    3786..3902
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="98.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             2548..2911
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3667..3767
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     promoter        1844..1870
                     /note="pLannotate"
                     /label="lac promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="87.1"
                     /fragment="True"
                     /other="promoter"
     CDS             1699..1791
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             7895..7963
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="91.3"
                     /match_length="23.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1801 aattaatgtg agttagctca ctcattaggc accccaggct ttccacttta tgcttccggc
     1861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1921 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     1981 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     2041 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     2101 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     2161 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     2221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2401 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2461 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2521 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2581 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2641 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2701 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     2761 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     2821 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     2881 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     2941 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     3001 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     3061 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     3121 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     3181 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     3241 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     3301 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3361 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3421 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3481 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3541 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3601 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     3661 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     3721 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     3781 ttaactttta aaagaaaagg ggggattggg gggtatagtg caggggaaag aatagtagac
     3841 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     3901 ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3961 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4021 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4081 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4141 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4201 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     4261 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4321 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     4381 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     4441 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     4501 actctagagg atccgccacc atggatgtat gcgtccgtct tgccctgtgg ctcctctggg
     4561 gactcctcct gcaccagggc cagagcctcg tgagcaaggg cgaggaggat aacatggcca
     4621 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     4681 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     4741 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     4801 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     4861 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     4921 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     4981 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     5041 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     5101 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     5161 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     5221 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     5281 acgagctgta caagagccat agtcacagtg agaaggcgac aggaaccagc tcgggggcca
     5341 actctgagga gtccactgca gcagagtttt gccgaattga caagcccctg tgtcacagtg
     5401 aggatgagaa actcagcttc gaggcagtcc gtaacatcca caaactgatg gacgatgatg
     5461 ccaatggtga tgtggatgtg gaagaaagtg atgagttcct gagggaagac ctcaattacc
     5521 atgacccaac agtgaaacac agcaccttcc atggtgagga taagctcatc agcgtggagg
     5581 acctgtggaa ggcatggaag tcatcagaag tatacaattg gaccgtggat gaggtggtac
     5641 agtggctgat cacatatgtg gagctgcctc agtatgagga gaccttccgg aagctgcagc
     5701 tcagtggcca tgccatgcca aggctggctg tcaccaacac caccatgaca gggactgtgc
     5761 tgaagatgac agaccggagt catcggcaga agctgcagct gaaggctctg gatacagtgc
     5821 tctttgggcc tcctctcttg actcgccata atcacctcaa ggacttcatg ctggtggtgt
     5881 ctatcgttat tggtgtgggc ggctgctggt ttgcctatat ccagaaccgt tactccaagg
     5941 agcacatgaa gaagatgatg aaggacttgg aggggttaca ccgagctgag cagagtctgc
     6001 atgaccttca ggaaaggctg cacaaggccc gggaggagca ccgcacagtg gaggtggaga
     6061 aggtccatct ggaaaagaag ctgcgcgatg agatcaacct tgctaagcag gaagcccagc
     6121 ggctgaagga gctgcgggag ggtactgaga atgagcggag ccgccaaaaa tatgctgagg
     6181 aggagttgga gcaggttcgg gaggccttga ggaaagcaga gaaggagcta gaatctcaca
     6241 gctcatggta tgctccagag gcccttcaga agtggctgca gctgacacat gaggtggagg
     6301 tgcaatatta caacatcaag aagcaaaatg ctgagaagca gctgctggtg gccaaggagg
     6361 gggctgagaa gataaaaaag aagagaaaca cactctttgg caccttccac gtggcccaca
     6421 gctcttccct ggatgatgta gatcataaaa ttctaacagc taagcaagca ctgagcgagg
     6481 cgacagcagc attgcgggag cgcctgcacc gctggcaaca gatcgagatc ctctgtggct
     6541 tccagattgt caacaaccct ggcatccact cactggtggc tgccctcaac atagacccca
     6601 gctggatggg cagtacacgc cccaaccctg ctcacttcat catgactgac gacgtggatg
     6661 acatggatga ggagattgtg tctcccttgt ccatgcagtc ccctagcctg cagagcagtg
     6721 ttcggcagcg cctgacggag ccacagcatg gcctgggatc tcagagggat ttgacccatt
     6781 ccgattcgga gtcctccctc cacatgagtg accgccagcg tgtggccccc aaacctcctc
     6841 agatgagccg tgctgcagac gaggctctca atgccatgac ttccaatggc agccaccggc
     6901 tgatcgaggg ggtccacccg gggtctctgg tggagaaact gcctgacagc cctgccccgg
     6961 ccaagaaggc attactggcg ctgaaccatg ggctggacaa ggcccacagc ctgatggagc
     7021 tgagcccctc agccccaccc ggtggctctc cacatttgga ttcttcccgt tctcacagcc
     7081 ccagctcccc agacccagac acaccatctc cagttgggga cagccgagcc ctgcaagcca
     7141 gccgaaacac acgcattccc cacctggctg gcaagaaggc tgtggctgag gaggataatg
     7201 gctctattgg cgaggaaaca gactccagcc caggccggaa gaagtttccc ctcaaaatct
     7261 ttaagaagcc tcttaagaag taggtcgaca atcaacctct ggattacaaa atttgtgaaa
     7321 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7381 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7441 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7501 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7561 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7621 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7681 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7741 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7801 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7861 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     7921 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7981 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8041 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8101 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8161 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8221 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8401 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8461 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8521 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     8581 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     8641 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8701 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8761 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8821 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8881 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8941 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9001 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9061 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9121 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9181 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     9241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9361 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9421 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct ga
//
