LOCUS       YFP-Stim1-deltaCTD                 6543 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(135..929)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3968..4684)
                     /note="pLannotate"
                     /label="EYFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1454..1882
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(964..1321)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(5004..5307)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(4800..5003)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1916..2037)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(1323..1427)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2201..3934)
                     /note="pLannotate"
                     /label="STIM1 (fragment)"
                     /database="swissprot"
                     /identity="99.8"
                     /match_length="84.4"
                     /fragment="True"
                     /other="CDS"
     rep_origin      complement(5482..6070)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(3934..3958)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     polyA_signal    complement(6399..6446)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(join(6505..6543,1..114))
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2161..2197)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="69.8"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2060..2144)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
       61 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
      121 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
      181 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
      241 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
      301 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
      361 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
      421 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
      481 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
      541 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
      601 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
      661 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
      721 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
      781 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
      841 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
      901 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
      961 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     1021 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     1081 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     1141 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     1201 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     1261 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     1321 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1441 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1501 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1801 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     1861 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcgttaaga
     1921 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1981 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2041 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2101 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt
     2161 ggatcccggg cccgcggtac cgtcgactgc agaattccta cagctccatc aggctgtggg
     2221 ccttgtccag cccatggttc agcgccagta atgccttctt ggccagggca gggctgtcag
     2281 gcagtttctc caccagagac cctgggtgga ccccctcgat cagccggtgg ctgccattgg
     2341 aagtcatggc attgagagcc tcgtctgcag cacggctcat ctgaggaggt ttgggggcca
     2401 cacgctggcg gtcactcatg tggagggagg actccgaatc ggaatgggtc aaatccctct
     2461 gagatcccag gccatgctgt ggctccgtca ggcgctgccg aacactgctc tgcaggctag
     2521 gggactgcat ggacaaggga gacacaatct cctcatccat gtcatccacg tcgtcagtca
     2581 tgatgaagtg agcagggttg gggcgtgtac tgcccatcca gctggggtct atgttgaggg
     2641 cagccaccag tgagtggatg ccagggttgt tgacaatctg gaagccacag aggatctcga
     2701 tctgttgcca gcggtgcagg cgctcccgca atgctgctgt cgcctcgctc agtgcttgct
     2761 tagctgttag aattttatga tctacatcat ccagggaaga gctgtgggcc acgtggaagg
     2821 tgccaaagag tgtgtttctc ttctttttta tcttctcagc cccctccttg gccaccagca
     2881 gctgcttctc agcattttgc ttcttgatgt tgtaatattg cacctccacc tcatgtgtca
     2941 gctgcagcca cttctgaagg gcctctggag cataccatga gctgtgagat tctagctcct
     3001 tctctgcttt cctcaaggcc tcccgaacct gctccaactc ctcctcagca tatttttggc
     3061 ggctccgctc attctcagta ccctcccgca gctccttcag ccgctgggct tcctgcttag
     3121 caaggttgat ctcatcgcgc agcttctttt ccagatggac cttctccacc tccactgtgc
     3181 ggtgctcctc ctgggccttg tgcagccttt cctgaaggtc atgcagactc tgctcagctc
     3241 ggtgtaaccc ctccaagtcc ttcatcatct tcttcatgtg ctccttggag taacggttct
     3301 ggatataggc aaaccagcag ccgcccacac caataacgat agacaccacc agcatgaagt
     3361 ccttgaggtg attatggcga gtcaagagag gaggcccaaa gagcactgta tccagagcct
     3421 tcagctgcag cttctgccga tgactccggt ctgtcatctt cagcacagtc cctgtcatgg
     3481 tggtgttggt gacagccagc cttggcatgg catggccact gagctgcagc ttccggaagg
     3541 tctcctcata ctgaggcagc tccacatatg tgatcagcca ctgtaccacc tcatccacgg
     3601 tccaattgta tacttctgat gacttccatg ccttccacag gtcctccacg ctgatgagct
     3661 tatcctcacc atggaaggtg ctgtgtttca ctgttgggtc atggtaattg aggtcttccc
     3721 tcaggaactc atcactttct tccacatcca catcaccatt ggcatcatcg tccatcagtt
     3781 tgtggatgtt acggactgcc tcgaagctga gtttctcatc ctcactgtga cacaggggct
     3841 tgtcaattcg gcaaaactct gctgcagtgg actcctcaga gttggccccc gagctggttc
     3901 ctgtcgcctt ctcactgtga ctatggctga gggagcctgc ttttttgtac aaacttgttg
     3961 atccggactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     4021 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tggtagctca
     4081 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     4141 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     4201 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     4261 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     4321 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     4381 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     4441 gctgcttcat gtggtcgggg tagcgggcga agcactgcag gccgtagccg aaggtggtca
     4501 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     4561 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     4621 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     4681 ccatggtggc gaccggtgcg aggctctggc cctggtgcag gaggagtccc cagaggagcc
     4741 acagggcaag acggacgcat acatccatgg tgctagcgga tctgacggtt cactaaacca
     4801 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     4861 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     4921 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     4981 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     5041 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     5101 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     5161 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     5221 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     5281 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     5341 cccgtaattg attactatta ataactaatg catggcggta atacggttat ccacagaatc
     5401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     6241 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     6301 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     6361 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     6421 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     6481 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     6541 ctg
//
