LOCUS       YFP-Stim1-D76A                 6859 bp    DNA     circular SYN 21-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(135..929)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4284..5000)
                     /note="pLannotate"
                     /label="EYFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1454..1882
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(964..1321)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(5320..5623)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(5116..5319)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1916..2037)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(1323..1427)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2259..4250)
                     /note="pLannotate"
                     /label="STIM1"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="96.9"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(2161..2226)
                     /note="pLannotate"
                     /label="MCS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(5798..6386)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(4250..4274)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2230..2254
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     polyA_signal    complement(6715..6762)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(join(6821..6859,1..114))
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2060..2144)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
       61 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
      121 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
      181 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
      241 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
      301 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
      361 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
      421 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
      481 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
      541 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
      601 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
      661 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
      721 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
      781 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
      841 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
      901 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
      961 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     1021 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     1081 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     1141 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     1201 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     1261 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     1321 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1441 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1501 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1801 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     1861 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcgttaaga
     1921 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1981 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2041 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2101 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt
     2161 ggatcccggg cccgcggtac cgtcgactgc agaattcgaa gcttgagctc gagatctgag
     2221 tccggatcaa ccactttgta caagaaagct gggttctact tcttaagagg cttcttaaag
     2281 attttgaggg gaaacttctt ccggcctggg ctggagtctg tttcctcgcc aatagagcca
     2341 ttatcctcct cagccacagc cttcttgcca gccaggtggg gaatgcgtgt gtttcggctg
     2401 gcttgcaggg ctcggctgtc cccaactgga gatggtgtgt ctgggtctgg ggagctgggg
     2461 ctgtgagaac gggaagaatc caaatgtgga gagccaccag gtggggctga ggggctcagc
     2521 tccatcaggc tgtgggcctt gtccagccca tggttcagcg ccagtaatgc cttcttggcc
     2581 ggggcagggc tgtcaggcag tttctccacc agagaccccg ggtggacccc ctcgatcagc
     2641 cggtggctgc cattggaagt catggcattg agagcctcgt ctgcagcacg gctcatctga
     2701 ggaggtttgg gggccacacg ctggcggtca ctcatgtgga gggaggactc cgaatcggaa
     2761 tgggtcaaat ccctctgaga tcccaggcca tgctgtggct ccgtcaggcg ctgccgaaca
     2821 ctgctctgca ggctagggga ctgcatggac aagggagaca caatctcctc atccatgtca
     2881 tccacgtcgt cagtcatgat gaagtgagca gggttggggc gtgtactgcc catccagctg
     2941 gggtctatgt tgagggcagc caccagtgag tggatgccag ggttgttgac aatctggaag
     3001 ccacagagga tctcgatctg ttgccagcgg tgcaggcgct cccgcaatgc tgctgtcgcc
     3061 tcgctcagtg cttgcttagc tgttagaatt ttatgatcta catcatccag ggaagagctg
     3121 tgggccacgt ggaaggtgcc aaagagtgtg tttctcttct tttttatctt ctcagccccc
     3181 tccttggcca ccagcagctg cttctcagca ttttgcttct tgatgttgta atattgcacc
     3241 tccacctcat gtgtcagctg cagccacttc tgaagggcct ctggagcata ccatgagctg
     3301 tgagattcta gctccttctc tgctttcctc aaggcctccc gaacctgctc caactcctcc
     3361 tcagcatatt tttggcggct ccgctcattc tcagtaccct cccgcagctc cttcagccgc
     3421 tgggcttcct gcttagcaag gttgatctca tcgcgcagct tcttttccag atggaccttc
     3481 tccacctcca ctgtgcggtg ctcctcctgg gccttgtgca gcctttcctg aaggtcatgc
     3541 agactctgct cagctcggtg taacccctcc aagtccttca tcatcttctt catgtgctcc
     3601 ttggagtaac ggttctggat ataggcaaac cagcagccgc ccacaccaat aacgatagac
     3661 accaccagca tgaagtcctt gaggtgatta tggcgagtca agagaggagg cccaaagagc
     3721 actgtatcca gagccttcag ctgcagcttc tgccgatgac tccggtctgt catcttcagc
     3781 acagtccctg tcatggtggt gttggtgaca gccagccttg gcatggcatg gccactgagc
     3841 tgcagcttcc ggaaggtctc ctcatactga ggcagctcca catatgtgat cagccactgt
     3901 accacctcat ccacggtcca attgtatact tctgatgact tccatgcctt ccacaggtcc
     3961 tccacgctga tgagcttatc ctcaccatgg aaggtgctgt gtttcactgt tgggtcatgg
     4021 taattgaggt cttccctcag gaactcatca ctttcttcca catccacatc accattggca
     4081 tcatcggcca tcagtttgtg gatgttacgg actgcctcga agctgagttt ctcatcctca
     4141 ctgtgacaca ggggcttgtc aattcggcaa aactctgctg cagtggactc ctcagagttg
     4201 gcccccgagc tggttcctgt cgccttctca ctgtgactat ggctgaggga gcctgctttt
     4261 ttgtacaaac ttgttgatcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg
     4321 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     4381 gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     4441 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     4501 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     4561 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     4621 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     4681 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     4741 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg
     4801 tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     4861 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     4921 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     4981 tcctcgccct tgctcaccat ggtggcgacc ggtgcgaggc tctggccctg gtgcaggagg
     5041 agtccccaga ggagccacag ggcaagacgg acgcatacat ccatggtgct agcggatctg
     5101 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     5161 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     5221 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     5281 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     5341 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     5401 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     5461 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     5521 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     5581 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     5641 gctatgaact aatgaccccg taattgatta ctattaataa ctaatgcatg gcggtaatac
     5701 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6541 aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac
     6601 ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg
     6661 gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa
     6721 acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
     6781 ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca
     6841 gcatgagatc cccgcgctg
//
