LOCUS       Exported               10679 bp ds-DNA     circular SYN 27-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Brady Cress
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 27, 2022 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    104..151
                     /label=lac Promoter (no lacO) Pt5
                     /note="color: #ff6600"
     promoter        104..134
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 104 .. 109 / #ffffff / -35
                        2: 110 .. 127 / #ffffff
                        3: 128 .. 134 / #ffffff / -10"
     RBS             135..146
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     CDS             153..845
                     /codon_start=1
                     /label=TnsA
                     /note="color: #993366"
                     /translation="MATSLPTPSAITTSALEYAFHTPARNLTKSRGKNIHRYVSVKMSK
                     RITVESTLECDACYHFDFEPSIVRFCAQPIRFLYYLNGQSHSYVPDFLVQFDTNEFVLY
                     EVKSAYAKNKPDFDVEWEAKVKAATELGLELELVEESDIRDTVVLNNLKRMHRYASKDE
                     LNNVHNSLLKIIKYNGAQSARCLGEQLGLKGRTVLPILCDLLSRCLLDTRLDKPLSLES
                     RFELASYG"
     CDS             838..2649
                     /codon_start=1
                     /label=TnsB
                     /note="color: #993366"
                     /translation="MAKKGFSSFHRKAVSSQDTLESIELVSSANCLESVTYQDISAFPE
                     TIAVEINFRLSILRFLARKCETIVAKSIEPHRVELQQNYSRKIPSAITIYRWWLAFRKS
                     DYNPISLAPNIKDRGNRETKVSTVVDSIMEQAVERVISGRKVNVSSAYKRVRRKVRQYN
                     LTHGTKYTYPKYESVRKRVKKKTPFELLAAGKGERVAKREFRRMGKKILTSSVLERVEI
                     DHTVVDLFAVHEEYRIPLGRPWLTQLVDCYSKAVIGFYLGFEPPSYVSVSLALKNAIQR
                     KDDLISSYESIENEWLCYGIPDLLVTDNGKEFLSKAFDQACESLLINVHQNKVETPDNK
                     PHVERNYGTINTSLLDDLPGKSFSQYLQREGYDSVGEATLTLNEIREIYLIWLVDIYHK
                     KPNQRGTNCPNVAWKKGCQEWEPEEFSGSKDELDFKFAIVDYKQLTKVGITVYKELSYS
                     NDRLAEYRGKKGNHKVQFKYNPECMAVIWVLDEDMNEYFTVNAIDYEYASRVSLWQHKY
                     NMKYQAELNSAEYDEDKEIDAEIKIEEIADRSIVKTNKIRARRRGARHQENSARAKSIS
                     NANPASIQKHEDEIVSADNDDWDIDYV"
     CDS             2661..3653
                     /codon_start=1
                     /label=TnsC
                     /note="color: #993366"
                     /translation="MSETREARISRAKRAFVSTPSVRKILSYMDRCRDLSDLESEPTCM
                     MVYGASGVGKTTVIKKYLNQNRRESEAGGDIIPVLHIELPDNAKPVDAARELLVEMGDP
                     LALYETDLARLTKRLTELIPAVGVKLIIIDEFQHLVEERSNRVLTQVGNWLKMILNKTK
                     CPIVIFGMPYSKVVLQANSQLHGRFSIQVELRPFSYQGGRGVFKTFLEYLDKALPFEKQ
                     AGLANESLQKKLYAFSQGNMRSLRNLIYQASIEAIDNQHETITEEDFVFASKLTSGDKP
                     NSWKNPFEEGVEVTEDMLRPPPKDIGWEDYLRHSTPRVSKPGRNKNFFE"
     CDS             3725..4909
                     /codon_start=1
                     /label=TniQ
                     /note="color: #993366"
                     /translation="MFLQRPKPYSDESLESFFIRVANKNGYGDVHRFLEATKRFLQDID
                     HNGYQTFPTDITRINPYSAKNSSSARTASFLKLAQLTFNEPPELLGLAINRTNMKYSPS
                     TSAVVRGAEVFPRSLLRTHSIPCCPLCLRENGYASYLWHFQGYEYCHSHNVPLITTCSC
                     GKEFDYRVSGLKGICCKCKEPITLTSRENGHEAACTVSNWLAGHESKPLPNLPKSYRWG
                     LVHWWMGIKDSEFDHFSFVQFFSNWPRSFHSIIEDEVEFNLEHAVVSTSELRLKDLLGR
                     LFFGSIRLPERNLQHNIILGELLCYLENRLWQDKGLIANLKMNALEATVMLNCSLDQIA
                     SMVEQRILKPNRKSKPNSPLDVTDYLFHFGDIFCLWLAEFQSDEFNRSFYVSRW"
     CDS             4910..6832
                     /codon_start=1
                     /label=Cas8
                     /note="color: #993366"
                     /translation="MQTLKELIASNPDDLTTELKRAFRPLTPHIAIDGNELDALTILVN
                     LTDKTDDQKDLLDRAKCKQKLRDEKWWASCINCVNYRQSHNPKFPDIRSEGVIRTQALG
                     ELPSFLLSSSKIPPYHWSYSHDSKYVNKSAFLTNEFCWDGEISCLGELLKDADHPLWNT
                     LKKLGCSQKTCKAMAKQLADITLTTINVTLAPNYLTQISLPDSDTSYISLSPVASLSMQ
                     SHFHQRLQDENRHSAITRFSRTTNMGVTAMTCGGAFRMLKSGAKFSSPPHHRLNSKRSW
                     LTSEHVQSLKQYQRLNKSLIPENSRIALRRKYKIELQNMVRSWFAMQDHTLDSNILIQH
                     LNHDLSYLGATKRFAYDPAMTKLFTELLKRELSNSINNGEQHTNGSFLVLPNIRVCGAT
                     ALSSPVTVGIPSLTAFFGFVHAFERNINRTTSSFRVESFAICVHQLHVEKRGLTAEFVE
                     KGDGTISAPATRDDWQCDVVFSLILNTNFAQHIDQDTLVTSLPKRLARGSAKIAIDDFK
                     HINSFSTLETAIESLPIEAGRWLSLYAQSNNNLSDLLAAMTEDHQLMASCVGYHLLEEP
                     KDKPNSLRGYKHAIAECIIGLINSITFSSETDPNTIFWSLKNYQNYLVVQPRSINDETT
                     DKSSL"
     CDS             6804..7862
                     /codon_start=1
                     /label=Cas7
                     /note="color: #993366"
                     /translation="MKLPTNLAYERSIDPSDVCFFVVWPDDRKTPLTYNSRTLLGQMEA
                     ASLAYDVSGQPIKSATAEALAQGNPHQVDFCHVPYGASHIECSFSVSFSSELRQPYKCN
                     SSKVKQTLVQLVELYETKIGWTELATRYLMNICNGKWLWKNTRKAYCWNIVLTPWPWNG
                     EKVGFEDIRTNYTSRQDFKNNKNWSAIVEMIKTAFSSTDGLAIFEVRATLHLPTNAMVR
                     PSQVFTEKESGSKSKSKTQNSRVFQSTTIDGERSPILGAFKTGAAIATIDDWYPEATEP
                     LRVGRFGVHREDVTCYRHPSTGKDFFSILQQAEHYIEVLSANKTPAQETINDMHFLMAN
                     LIKGGMFQHKGD"
     CDS             7865..8464
                     /codon_start=1
                     /label=Cas6
                     /note="color: #993366"
                     /translation="VKWYYKTITFLPELCNNESLAAKCLRVLHGFNYQYETRNIGVSFP
                     LWCDATVGKKISFVSKNKIELDLLLKQHYFVQMEQLQYFHISNTVLVPEDCTYVSFRRC
                     QSIDKLTAAGLARKIRRLEKRALSRGEQFDPSSFAQKEHTAIAHYHSLGESSKQTNRNF
                     RLNIRMLSEQPREGNSIFSSYGLSNSENSFQPVPLI"
     misc_feature    8483..8510
                     /label=V. cholerae CRISPR Repeat
                     /note="color: #000000; direction: BOTH"
     misc_feature    8506..8509
                     /label=Guide BsaI_OH
                     /note="color: #339966"
     misc_feature    8511..8535
                     /label=Vc_2xBsaI_NT (Guide Stuffer)
                     /note="color: #00ff00"
     misc_feature    complement(8511..8516)
                     /label=BsaI
                     /note="color: #000000; direction: LEFT"
     misc_feature    8530..8535
                     /label=BsaI
                     /note="color: #000000; direction: RIGHT"
     misc_feature    8536..8563
                     /label=V. cholerae CRISPR Repeat
                     /note="color: #000000; direction: BOTH"
     misc_feature    8537..8540
                     /label=Guide BsaI_OH
                     /note="color: #339966"
     misc_feature    complement(8589..8696)
                     /label=Tn7 Left End
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    8589..8596
                     /label=8 bp terminal end
                     /note="color: #ffff99"
     misc_feature    complement(8597..8616)
                     /label=20 bp putative TnsB binding site
                     /note="color: #527a9d; direction: LEFT"
     misc_feature    complement(8651..8670)
                     /label=20 bp putative TnsB binding site
                     /note="color: #527a9d; direction: LEFT"
     misc_feature    complement(8677..8696)
                     /label=20 bp putative TnsB binding site
                     /note="color: #527a9d; direction: LEFT"
     misc_feature    8701..8732
                     /label=2xLguI Cargo Stuffer
                     /note="This feature has 3 segments:
                        1: 8701 .. 8703 / #99cc00 / OH
                        2: 8704 .. 8729 / #00ff00 / 2xLguI Cargo Stuffer
                        3: 8730 .. 8732 / #99cc00 / OH"
     misc_feature    complement(8705..8711)
                     /label=LguI
                     /note="color: #000000; direction: LEFT"
     misc_feature    8722..8728
                     /label=LguI
                     /note="color: #000000; direction: RIGHT"
     promoter        8737..8755
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    8757..8760
                     /label=Barcode BsmBI_OH
                     /note="color: #ffcc00"
     misc_feature    8761..8784
                     /label=2xBsmBI Barcode Stuffer
                     /note="color: #ff9900"
     misc_feature    complement(8762..8767)
                     /label=BsmBI
                     /note="color: #000000; direction: LEFT"
     misc_feature    8778..8783
                     /label=BsmBI
                     /note="color: #000000; direction: RIGHT"
     misc_feature    8785..8788
                     /label=Barcode BsmBI_OH
                     /note="color: #ffcc00"
     misc_feature    8789..8859
                     /label=Tn7 Right End
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    8789..8808
                     /label=20 bp putative TnsB binding site
                     /note="color: #527a9d; direction: RIGHT"
     misc_feature    8812..8831
                     /label=20 bp putative TnsB binding site
                     /note="color: #527a9d; direction: RIGHT"
     misc_feature    8832..8851
                     /label=20 bp putative TnsB binding site
                     /note="color: #527a9d; direction: RIGHT"
     misc_feature    8852..8859
                     /label=8 bp terminal end
                     /note="color: #ffff99"
     misc_feature    8900..8907
                     /label=AsiSI for ET-Seq
                     /note="color: #ff00ff; direction: BOTH"
     misc_feature    8923..8930
                     /label=SbfI for ET-Seq
                     /note="color: #ff00ff; direction: BOTH"
     oriT            complement(8942..9051)
                     /direction=LEFT
                     /label=oriT (direction indicates 5' end leading transfer)
                     /note="incP origin of transferdirectionality deduced by BFC
                     from plasmid R751 and ""Complete Nucleotide Sequence of 
                     Birmingham IncPa plasmids"" paper"
                     /note="color: #a6acb3"
     misc_feature    8943..9186
                     /label=oriT
                     /note="color: #ff00ff"
     promoter        9244..9348
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     -35_signal      9279..9284
                     /label=bla promoter (counter-clockwise)
                     /label=bla promoter (counter-clockwise); TTCAAA
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /note="color: #ffc0cb"
     -10_signal      9300..9305
                     /label=bla promoter (counter-clockwise)
                     /label=bla promoter (counter-clockwise); GAGACA
                     /note="bla promoter (counter-clockwise); GAGACA"
                     /note="color: #ffc0cb"
     CDS             9349..10209
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampR (confers resistance to ampicillin)"
                     /note="color: #ffc0cb"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    10219..10637
                     /label=R6K origin of replication
                     /label=R6K origin of replication_pJW20
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
                     /note="This feature has 3 segments:
                        1: 10219 .. 10244 / #00ff00 / also annotated on R6K
                        2: 10245 .. 10633 / #ffff00 / R6K
                        3: 10634 .. 10637 / #00ff00 / also annotated on R6K"
ORIGIN
        1 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
       61 taacgtgagt tttcgttcca ctgacacctg cccgaaccat atctttacac tttatgcttc
      121 cggctcgtat gttgaaagag gagaaaggat caatggcgac aagtttacct acgccctcag
      181 caattacgac ttcggcgtta gagtatgcat tccatactcc cgctcgcaat ctaacgaaat
      241 ctcgcggaaa aaacattcat cgttatgtca gtgtaaagat gagtaagagg attacggtag
      301 aatctactct agagtgtgat gcctgctatc actttgattt tgagccaagt attgttcgct
      361 tttgcgctca accgattcga tttttatatt atctcaatgg tcagtctcac tcctatgttc
      421 ctgactttct agttcaattt gataccaacg agtttgttct atatgaagta aagtcagctt
      481 atgctaagaa caaacctgat tttgatgttg aatgggaggc gaaagtaaaa gcagcaactg
      541 aactagggct agaattggag cttgttgaag agagtgatat tagggatacg gttgtattaa
      601 ataatcttaa gcgcatgcat cgttatgctt cgaaagatga gctgaataac gtacataact
      661 ctctcttaaa aataataaag tacaatggcg cccaatctgc aagatgcttg ggagaacagt
      721 tgggtttaaa aggccgaact gttttaccaa ttttgtgcga tttgctgtca aggtgtttac
      781 tcgatacacg tttggataag cctctatctc ttgaatctcg atttgagttg gccagttatg
      841 gctaagaaag ggttctcaag tttccataga aaagcagtct cttcccaaga tacgcttgaa
      901 tcgatagagc ttgtctctag cgctaattgt ctagaaagtg ttacgtatca agatatatca
      961 gcatttcccg aaacaattgc ggtagagatt aatttccgat taagcattct tcgcttttta
     1021 gcgcgaaagt gcgaaaccat tgtggccaaa tcaattgaac cacatcgtgt agagctacag
     1081 caaaactata gtagaaaaat acccagtgca ataacgatat atcgatggtg gcttgctttt
     1141 cgaaaatcag actacaaccc cattagctta gcacctaata tcaaggatag aggtaataga
     1201 gagacaaaag tgtcaacagt tgttgattct attatggaac aggcagttga aagagttata
     1261 tctggacgaa aagtcaatgt tagctctgca tataaacgtg ttcgacgaaa agttcgtcaa
     1321 tacaatctaa ctcatggaac gaaatacacg tatcctaagt acgaatctgt aagaaagcga
     1381 gtaaaaaaga aaaccccatt tgagttatta gccgcaggga aaggggagag agtagctaag
     1441 agagagtttc gccgaatggg aaaaaagatc ctcacgtcta gcgtgctaga gagggttgaa
     1501 atagatcaca ctgtcgttga cctttttgca gtacacgaag agtatcgaat cccattgggc
     1561 cgaccttggc ttactcaatt ggttgattgt tacagtaaag ctgtaatcgg tttttattta
     1621 ggtttcgagc ctcctagcta tgtgtcggtt tcccttgcac ttaagaatgc aatacaacgc
     1681 aaagatgact taatctcctc gtatgaatcg atcgagaatg aatggctatg ttatggcatc
     1741 ccagacctac tcgtaactga taatggtaaa gagtttttgt cgaaagcatt tgatcaagca
     1801 tgtgaatcac tattgatcaa tgtgcatcaa aataaagttg aaacgcccga caacaaacct
     1861 catgttgaac gtaactacgg gactattaat acttctctgt tagacgattt acctgggaaa
     1921 tccttcagcc agtaccttca aagagaaggg tacgactctg tgggagaagc tacccttaca
     1981 ctcaatgaga ttagagaaat ttacttaatt tggttggtgg atatttatca taaaaaaccc
     2041 aatcagagag gcactaattg tcctaatgtt gcgtggaaaa agggttgtca agaatgggaa
     2101 ccagaggagt tctctggttc taaagacgaa ttagacttta aatttgctat tgttgattac
     2161 aaacaactta ctaaagtagg gataactgtc tacaaagaac tgagttatag caatgaccgt
     2221 ttagctgaat atagagggaa gaaaggaaac cataaagttc agttcaagta taaccctgag
     2281 tgtatggcag ttatttgggt gttggatgag gatatgaatg agtactttac agttaatgcg
     2341 attgactacg aatatgcaag tagagtatca ctttggcaac ataaatataa catgaaatat
     2401 caagcagaac taaattcagc agaatatgat gaggacaagg aaattgatgc agaaataaaa
     2461 attgaagaaa tcgcagatcg ttcaattgtt aagactaaca aaatcagagc tcggaggcgt
     2521 ggcgctaggc atcaagagaa tagcgcaagg gctaagtcaa tcagtaatgc gaacccggcc
     2581 tcgatacaaa aacatgaaga tgaaatcgtt agtgcagata atgacgattg ggatattgat
     2641 tatgtctgag aaatcgtcaa atgagtgaaa cgcgtgaggc tcgtatatca agagctaaaa
     2701 gggcatttgt atccacaccg agcgttagga aaatcttgag ttacatggat agatgtagag
     2761 atctatcaga cctagagtct gagcctacat gcatgatggt ctatggtgct tcgggtgtag
     2821 gtaaaacgac cgtcatcaag aaatacttaa atcaaaacag aagagagtcc gaagccgggg
     2881 gcgatataat accggttttg catattgagt tgccagacaa tgcgaagcca gtagatgcag
     2941 caagggaatt gctggttgaa atgggtgacc cgctagcact ttatgaaact gacttagcta
     3001 gattgacgaa aagactgact gaattaatcc ctgcggtcgg cgtgaagctg attattatcg
     3061 atgagttcca acatttggtg gaagaaaggt caaatcgggt tcttacccaa gtaggtaatt
     3121 ggctaaaaat gatacttaac aaaacgaaat gtccaattgt tatatttggt atgccatact
     3181 caaaagttgt actgcaagca aactcgcaac ttcacgggcg attttccatt caggttgaac
     3241 tgcgcccctt tagctaccag ggaggtagag gtgtatttaa aacttttttg gaataccttg
     3301 ataaagccct accttttgaa aaacaggctg gcttagccaa cgaaagtttg cagaaaaaat
     3361 tgtatgcatt ctctcaggga aacatgcgtt cgttgagaaa ccttatttat caagcatcta
     3421 tcgaagcaat tgataatcag catgaaacga taaccgaaga agatttcgtt tttgcatcga
     3481 agttgacatc gggcgataaa cccaactcat ggaaaaatcc ttttgaggag ggtgttgagg
     3541 taacagaaga tatgttacga ccgccaccaa aagatattgg ttgggaggac tatttgagac
     3601 attcaacccc gagagtgagt aaaccaggta gaaataaaaa ctttttcgaa taacctaggc
     3661 tgctgccacc gctgagcaat aaccatctta gtatattagt taagtataag aaggagatat
     3721 acatatgttt ttgcaaagac ctaaacctta cagcgatgaa agtttagaaa gtttctttat
     3781 ccgagtggct aacaaaaatg gctacggtga tgtccatcgc ttcctagaag ccactaaacg
     3841 attccttcaa gacattgacc ataatggcta tcaaaccttt ccgactgata taactcggat
     3901 aaacccatac tcagctaaaa acagttccag cgcacgaact gcgtcattcc tgaaacttgc
     3961 acaattgaca tttaatgaac cgccagagct acttgggttg gcaattaaca gaacaaacat
     4021 gaaatactcg ccgtcaacta gcgcggttgt tagaggtgca gaagtctttc ctcgcagttt
     4081 actacggacg cactccatcc cctgctgtcc tttgtgtctg cgagaaaatg gctacgcctc
     4141 ctacctttgg cactttcagg ggtacgaata ctgccacagc cataacgtac ctttaattac
     4201 cacttgtagc tgtggtaagg agtttgacta ccgagtatct gggttaaagg gcatttgctg
     4261 caaatgcaag gagcctatca ccttaaccag cagggagaac ggtcatgagg cagcgtgtac
     4321 tgtttcaaac tggcttgctg gccatgaatc taaacctctg ccaaatcttc ctaaaagcta
     4381 ccgatggggt ttagttcatt ggtggatggg tattaaagat agcgagttcg atcacttttc
     4441 gttcgttcaa tttttctcaa actggccaag gtcattccac tcgataatcg aagatgaagt
     4501 agagttcaac cttgagcatg ctgttgtcag cacgtctgaa ttacgactaa aagatcttct
     4561 tggtcgattg tttttcggtt caattcggtt acctgagcgg aatcttcaac acaatatcat
     4621 ccttggtgag cttctctgct atttagaaaa tcgtttatgg caagacaagg gattaatcgc
     4681 caacctcaaa atgaacgcgt tagaggcgac tgtaatgtta aattgtagcc tcgatcagat
     4741 tgcatcaatg gttgaacaac gcatcttgaa gccaaatcga aaaagcaagc ccaacagccc
     4801 tcttgatgtt accgattatc tatttcattt cggcgatatt ttctgtcttt ggttagctga
     4861 gttccaaagc gatgagttta accgttcgtt ttatgtgtcg aggtggtaaa tgcaaactct
     4921 gaaagaacta atcgcatcca atcctgacga cttaacaact gaacttaaga gagcatttcg
     4981 tccactcaca ccgcatattg caattgatgg taatgaactt gacgcactga cgatattagt
     5041 caatttaacc gataagactg atgatcagaa agacctgctc gatcgagcca aatgcaagca
     5101 aaaacttcga gatgaaaaat ggtgggctag ctgcataaat tgcgttaatt acagacaaag
     5161 ccataaccca aaattcccgg atatacgttc tgaaggcgtg attcgaaccc aagccctggg
     5221 tgaattaccg agtttcctac tctcgtcctc aaaaatccca ccataccatt ggtcatatag
     5281 ccatgattca aaatacgtca acaaaagcgc attcctcacc aatgagtttt gttgggatgg
     5341 tgagatctca tgtttgggtg agcttcttaa agatgcagat cacccacttt ggaatacttt
     5401 gaaaaagtta ggttgttctc aaaaaacttg caaagcaatg gcaaaacaac tagctgatat
     5461 tactctcacg actatcaatg tcacactcgc accaaattac ctgactcaaa tatctctccc
     5521 cgatagtgat acatcctaca tttcactctc tcctgttgca tcgctatcga tgcaaagcca
     5581 ctttcatcag aggctccaag atgaaaatcg gcatagcgcg ataacgcggt ttagccgaac
     5641 taccaacatg ggggtcactg cgatgacatg tggcggcgca tttagaatgt taaagtctgg
     5701 cgctaagttt tccagccccc ctcatcaccg attaaacagt aaacgaagtt ggttgacgtc
     5761 agagcatgtt cagtcattaa aacagtacca gcgcctcaac aaaagcctca tacctgaaaa
     5821 ctctcggatt gcactccgta gaaaatacaa aatcgagctt caaaatatgg tcagatcttg
     5881 gtttgcaatg caagaccata cccttgattc gaacatactt atccaacatt tgaatcatga
     5941 cctatcttac ttaggagcca caaaacgttt tgcatacgat ccagcgatga ccaagctctt
     6001 tactgagctt ttgaaacgag agttatcaaa ttcaatcaat aatggtgagc aacacactaa
     6061 tggatcgttt ttagtcctac cgaatatcag ggtttgtggc gcaacagctt taagctcccc
     6121 ggtaacggtg gggattccat cacttacagc tttctttggc ttcgttcacg catttgaacg
     6181 gaatataaat cgcaccacct catcgtttcg tgttgaatcc tttgcgatat gcgtccatca
     6241 actacatgtc gagaagcgag gtttgacagc agagtttgtg gaaaaaggcg acgggactat
     6301 atccgctccc gcgacccggg atgactggca gtgtgatgtc gtatttagcc ttattttgaa
     6361 caccaacttt gctcaacata ttgaccaaga tacgttagtt acatcactac caaagcgatt
     6421 ggctcggggt tcagcaaaaa ttgcgattga tgactttaaa catatcaact cattctcgac
     6481 attagaaaca gcgatcgaat ctctgccaat agaagctggt aggtggttat cactttacgc
     6541 acagtcaaac aataatctaa gtgatctatt agcagccatg acagaggacc atcagctcat
     6601 ggcaagctgc gtcggttacc acttgttaga agaacccaaa gataaaccaa actccctcag
     6661 aggttacaaa cacgctatcg ccgagtgcat cattggactc attaactcaa tcacctttag
     6721 ctcagagact gatcccaaca caatcttttg gtcgctaaag aactatcaaa actacctagt
     6781 ggtacagcca aggagtatca acgatgaaac taccgacaaa tctagcctat gagcgctcta
     6841 tcgacccatc agatgtctgt ttttttgtcg tctggcccga tgatagaaaa acacctttaa
     6901 cctacaattc tcgtactctg ctcgggcaaa tggaagcggc atcattagcc tatgatgtct
     6961 caggtcaacc aataaaaagt gccaccgctg aggcgttagc tcaagggaac cctcatcaag
     7021 ttgatttctg ccacgttcca tacggtgcga gtcatattga atgcagtttc tccgtttcgt
     7081 tttcttctga actacgtcaa ccatataagt gtaactcaag caaagttaaa caaacgctag
     7141 tgcaattagt cgagctctac gaaacgaaaa tcggctggac tgagctagca acccgatatt
     7201 tgatgaacat ttgcaacggt aaatggctgt ggaaaaatac ccgtaaagcc tattgctgga
     7261 acattgtact tacaccttgg ccttggaacg gggaaaaggt tggatttgaa gatattcgta
     7321 ctaactacac ctcacggcaa gactttaaaa ataataaaaa ttggtctgct atagttgaaa
     7381 tgatcaaaac cgcattttct agtactgatg ggctggcgat atttgaagtc agggccacct
     7441 tgcacttgcc aacgaatgct atggtgcggc caagccaagt tttcacagaa aaagaaagtg
     7501 gcagtaaaag taaatctaaa actcaaaaca gtcgagtttt tcagagtaca actattgatg
     7561 gtgaacgatc gccaatacta ggggccttta aaacgggagc agctattgca accattgacg
     7621 actggtatcc tgaagccact gagccactaa gggtcggacg gtttggggtt catcgcgaag
     7681 atgtcacttg ctaccgtcat ccgtctaccg gaaaagattt tttctcgata ttacaacaag
     7741 cagagcacta tattgaagtg ttgagcgcca acaaaactcc cgctcaagaa actatcaacg
     7801 acatgcactt tttaatggct aacctgatta agggtgggat gttccagcat aaaggagact
     7861 gactgtgaaa tggtattata agacaatcac ctttctgcca gagttgtgca acaacgagtc
     7921 actggctgca aagtgtctcc gcgttctgca tggatttaac tatcagtatg agacacgaaa
     7981 tatcggcgtt tcatttccgc tttggtgtga tgcaacggtt ggaaaaaaga tttcatttgt
     8041 cagcaagaac aagatagaac tcgacttact acttaaacaa cactatttcg tccaaatgga
     8101 acaacttcaa tactttcata tatccaacac tgttctcgtc ccagaagatt gtacatacgt
     8161 ttcctttaga cgctgtcaat ctatagataa gctcacagca gcagggctgg caaggaaaat
     8221 cagacgcctg gagaaacgtg ctctatctag aggcgagcaa tttgacccat catcttttgc
     8281 tcaaaaagag catactgcaa tagcgcacta ccactcactt ggggagtcca gcaaacagac
     8341 gaaccgcaac tttcgactca atatcaggat gctctcggag caaccccgtg aggggaactc
     8401 gatttttagt agctatggct tatcaaattc agaaaactcg tttcagcctg tacccttaat
     8461 ctgacctagg ctgctgccac cggtgaactg ccgagtaggt agctgataac gagacctcgt
     8521 ttacctatcg gtctcgtgaa ctgccgagta ggtagctgat aacccaaacc agatgtcaac
     8581 acagctactg ttgatgcaac cataaagtga tatttaataa ttatttataa tcagcaactt
     8641 aaccacaaaa caaccatata ttgatatctc acaaaacaac cataagttga tatttttcag
     8701 aaaggaagag cgagatctcg agctcttcgg ctgctgtaat acgactcact ataggggata
     8761 agagacggct caacgcccgt ctcacagcgc aatatcaatt tatgggtgtg ataattatca
     8821 atttatgggt gtaattatca ttttatggtt gtatcaacaa ggaggacttt ggcaggtgca
     8881 gcacaaaagg aaactcaccg cgatcgccat tactcgcatc agcctgcagg attcttgctt
     8941 atcggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     9001 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     9061 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     9121 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     9181 gaaaagaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     9241 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9421 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9481 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9541 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9661 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9721 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9781 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    10021 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt
    10081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10201 cattggtaac tgtcagaccc ggccacgatg cgtccggcgt agaggatctg aagatcagca
    10261 gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
    10321 taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct
    10381 catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa
    10441 aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa
    10501 agaatatata aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga
    10561 tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac
    10621 ttaacggctg acatgggcaa gtttactcat atatacttta gattgattta aaacttcat
//
