LOCUS       Exported                6745 bp ds-DNA     circular SYN 28-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Fra
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 23, 2022 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    123..177
                     /label=PpsbA2*
     misc_feature    184..196
                     /label=RBS B0032
     misc_feature    197..202
                     /label=Scar
     CDS             203..1564
                     /codon_start=1
                     /label=CYP110D1
                     /translation="MTVTQNLPNGPRIPRLLRLFKFITQPIQYVEDFAKVYGDNFTIWG
                     SGESYFVYFSHPQALEQIFTNVSCFESSGGGSPLLELLLGKNSLILLEGDRHQRQRQLL
                     TPPFHGERMRAYGQTIREITQQVTQAWQMGKPFNIRASMQEITMRVILRVVFGVDEGEL
                     FQELRQLLTTLLDFMGSPLMSSTFFFSFTQKDYGAWSPWGRMVRLIKKIDQLIYALIAQ
                     RRAEFGENRQDILSLLISARYDDGQPMSDVELRDELMTMLVAGHETTASALTWAFYWID
                     SVPEVREKLFQELDTLNDDSEPSIIAKLPYLTAVCQETLRFYPIVLNAFFRRTKNPMEI
                     MGYKLPKATLVVPSIYLAHHREEVYPQSKQFRPERFLEKQFSPYEYLPFGGGNRRCIGL
                     AFAQYEMKIVLATILSQFQVSRLSKRPVQPVRRGLTLAAPGGMKMVANKRMRNS"
     primer_bind     complement(1538..1573)
                     /label=Primer fastcloning 1
     primer_bind     1573..1607
                     /label=fast cloning 2
     terminator      1625..1719
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1851..2666
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2833..2942
                     /note="incP origin of transfer"
     CDS             complement(2952..3803)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3790..4629)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5140..6111)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     promoter        complement(6135..6165)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     rep_origin      6221..6615
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      6653..6739
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccgggcta attagggggt gtcgcccttt gaattcgcgg ccgcttctag
      121 agagctttac aaaactctca ttaatccttt agactaagtt tagtcagttc caatctgtac
      181 tagtcacaca ggaaagtact agatgacagt cactcaaaac ctacccaacg gccccagaat
      241 accccggttg ttacggttgt ttaagtttat tactcaacca atacagtatg tggaagattt
      301 tgccaaagtc tacggtgaca acttcactat ctgggggagt ggagaaagtt attttgtcta
      361 ctttagccat ccccaagcat tggaacagat ttttactaat gttagttgtt ttgagagtag
      421 tggtggaggt agtccgcttt tagaactttt attgggtaag aattctttaa ttttactaga
      481 aggcgatcgc catcagcgcc aacgtcaact attaacccca cctttccacg gtgaaaggat
      541 gcgggcttat ggtcaaacta ttcgtgaaat aactcaacaa gtcactcaag catggcaaat
      601 gggtaagccc ttcaatatcc gggcttccat gcaggaaatt accatgcgtg tcattttgcg
      661 ggtagtgttt ggtgttgatg agggggaatt gtttcaagaa ctgcggcaat tgctaactac
      721 cctgttagat ttcatgggtt ctcctctaat gtccagcacc ttctttttta gtttcacgca
      781 aaaggactat ggtgcatgga gtccttgggg gcgaatggtg cgcctcatca aaaaaattga
      841 ccaactgatt tacgctttga ttgctcaacg tcgggctgag tttggcgaaa atcgtcaaga
      901 tatccttagt ttattaattt cggcacgtta tgacgacgga cagccaatgt cagacgtaga
      961 gttacgggat gaattaatga caatgttggt tgcaggacat gaaaccactg cttcagcctt
     1021 gacttgggcg ttttattgga ttgatagcgt accagaagta cgtgaaaagt tatttcagga
     1081 attagataca ctgaatgacg attcagaacc cagtattatt gccaaattgc cttatttaac
     1141 agcagtttgc caagaaaccc tacgctttta tcctattgtg ctgaatgctt tcttccggcg
     1201 aacaaaaaac cctatggaaa ttatgggcta caaactgccg aaagcaacat tagttgttcc
     1261 tagtatttat ttagcccatc atcgagaaga agtttatccg caatccaagc aattccgacc
     1321 agaacgcttt ttagaaaagc aattctcccc ttacgaatac ttaccctttg gcggcggtaa
     1381 tcggcgctgc atcggtctag catttgccca atatgaaatg aaaattgtct tagcaacaat
     1441 tttgtcacaa ttccaagtat ctagactcag taagcgtccg gtgcagcctg tgcgtcgggg
     1501 attaactttg gcagcaccag gaggaatgaa gatggtggct aataaaagaa tgcgtaattc
     1561 gtaactgcag gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt
     1621 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1681 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
     1741 gatttaaatt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1801 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1861 ttcagcgtga aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt
     1921 atggctataa atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt
     1981 atggcaaacc ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg
     2041 atgtgaccga tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca
     2101 ttaaacattt tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca
     2161 aaaccgcgtt tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc
     2221 tggccgtgtt tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg
     2281 atcgtgtgtt tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga
     2341 gcgattttga tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata
     2401 aactgctgcc gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc
     2461 tgattttcga tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg
     2521 atcgttatca ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc
     2581 aaaaacgtct gtttcagaaa tatggcattg ataatccgga tatgaacaaa ctgcaatttc
     2641 atctgatgct ggatgaattt ttctaataat taattggacc gcggtccgcg cgttgtcctt
     2701 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     2761 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     2821 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     2881 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     2941 gtaggccggc ctcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac
     3001 tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg
     3061 cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct
     3121 atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg
     3181 cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg
     3241 ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc
     3301 tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg
     3361 atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca
     3421 ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccacatgg
     3481 cattcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct
     3541 tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct
     3601 tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg
     3661 ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg
     3721 gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gccggacgtg gctgaggctg
     3781 tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc tctccagcac
     3841 ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc
     3901 atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc
     3961 ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc ggatgttatc
     4021 gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca tcatggccgc
     4081 gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg cgatggcctc
     4141 catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga tgtggaaccg
     4201 gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa
     4261 ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca gcaggccgtc
     4321 agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg
     4381 aatggcggtg ggcgggtctt cggcgggcag gtagatcacc gggccggtgg gcagttcgcc
     4441 cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga
     4501 tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca tgttgggcaa
     4561 aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga taggcttatg
     4621 ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc ggggtcacta
     4681 cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg
     4741 tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc
     4801 gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc cttttggtct
     4861 ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga
     4921 caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc ggccagcctc
     4981 ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt cacttttgat
     5041 caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt ctgtgcgtga
     5101 gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga aatctggccc
     5161 gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag
     5221 ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg
     5281 gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc cgatttcctc
     5341 ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact tgctgaggtc
     5401 atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg
     5461 ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg gccagcctgc gggccttctc
     5521 ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg ccgggccagc
     5581 ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac cggcgcgggt
     5641 ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc
     5701 gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc cccgaagttc
     5761 accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc tggtttccac
     5821 taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact cgctgaggtc
     5881 gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca cgtcattgcc
     5941 ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg ctgaccattc
     6001 ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc gctcacagcc
     6061 catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca tcgggccacc
     6121 aagcgattcc cacacattat acgagccgga agcataaagt gtaaagccta gatccgaagg
     6181 atgagccggg ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac gcgcccccac
     6241 ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg
     6301 ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc agcggtgggg
     6361 cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg ggcatattgg
     6421 gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat tattcttaga
     6481 taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg gtttgcaggt
     6541 ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt
     6601 tattgcaggg gttcgtgaca gttagggcgc gcccagctgt ctagggcggc ggatttgtcc
     6661 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
     6721 gagcctttcg ttttatttga tgcct
//
