LOCUS       pNHT7::RPL15::sNluc1        2951 bp    DNA     circular UNA 28-JAN-2021
DEFINITION  pNHT7 with RPL15 5' and 3' UTRs flanking sNluc1 .
ACCESSION   urn.local...69-cq57s4i
VERSION     urn.local...69-cq57s4i
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>553,<1216..2951)
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     terminator      268..295
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /label="rrnB T2 terminator"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /label="Escherichia coli rrnB terminator"
     primer_bind     537..553
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 fwd primer bind"
     misc_feature    <550..553
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        550..553
                     /label="Ligation"
     source          join(complement(<1210..>1219),complement(<550..>572))
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /label="source Cloning vector pUC19"
     source          <550..>553
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     promoter        554..572
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     source          complement(<554..>572)
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    569..572
                     /created_by="richardhall"
                     /label="T1"
     ligation        569..572
                     /label="Ligation"
     5'UTR           573..659
                     /created_by="nhall"
                     /label="RPL15 5' UTR"
     CDS             660..1175
                     /created_by="richardhall"
                     /modified_by="nhall"
                     /label="sNluc1"
     misc_feature    660..1176
                     /Source="referenceToDocument: urn:local:.:1lz-c8ptu53"
                     /label="Digestion of pENTR_L1_smed-NanoLuc_L2 -
                     smed-NanoLuc - Fragment 2 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    660..>663
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        660..663
                     /label="Ligation"
     misc_feature    890
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1173..663
                     /Source="referenceToDocument: urn:local:.:1lu-c8ptu53"
                     /label="Digestion of pNHT7::RPL15 extraction - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <1173..1176
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        1173..1176
                     /label="Ligation"
     misc_feature    1176
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     3'UTR           1176..1209
                     /created_by="nhall"
                     /label="RPL15 3' UTR"
     misc_feature    1210..1215
                     /created_by="richardhall"
                     /label="HindIII"
     misc_feature    <1210..>1219
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        1210..1213
                     /label="Ligation"
     source          complement(<1210..>1215)
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /label="source Cloning vector pUC19"
     primer_bind     complement(1216..1232)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev primer bind"
     ligation        1216..1219
                     /label="Ligation"
     source          <1216..>1219
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             1345..2154
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418
                      (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="aph(3')-Ia CDS"
     rep_origin      2301..2889
                     /direction="RIGHT"
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori rep origin"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agttaatacg actcactata ggattttact aaaatcaaaa ttaattatcg
      601 gtaaacttaa actttgttta aataaataat ttcaaaatta taaaaaatat ttaaaaacaa
      661 tggtttttac attagaagat tttgttggag attggagaca aacagctgga tataatttag
      721 atcaagtttt agaacaagga ggagtttcat cattgttcca aaatttggga gtttcagtta
      781 caccaattca aagaattgtt ttgagtggag aaaatggatt aaagattgat attcatgtta
      841 ttattccata tgagggatta tcaggagatc aaatgggaca aattgaaaag attttcaaag
      901 ttgtttatcc agttgatgat catcatttca aagttatttt acattatgga acattagtta
      961 ttgatggagt tacaccaaat atgattgatt attttggaag accatatgag ggaattgctg
     1021 tttttgatgg aaagaagatt acagttacag gaacattatg gaatggaaac aaaattattg
     1081 acgaaagatt aattaatcca gatggatcat tattatttag agttacaatt aatggagtta
     1141 caggatggag attatgtgaa agaattttag cttaatgact aatattaaat aaaactaatt
     1201 ggaattgtta agcttgtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1261 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1321 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1381 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1441 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1501 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1561 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1621 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1681 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1741 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1801 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1861 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1921 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1981 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2041 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2101 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2161 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2221 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2941 gctcacatgt t
//
